191 research outputs found
Characterization of aging hair and its influence in quality of life
There are few studies that characterize the non-pigmented hair compared to pigmented hair. Furthermore, it is important to evaluate how the natural process of aging hair influences quality of life in women. For this purpose, a questionnaire was applied to evaluate the impact of greying hair and the appearance alterations on womenÂŽs self-esteem. The satisfaction level of volunteers regarding their hair structure and conditions such as shine, strength and smoothness were also evaluated. Mechanical properties, such as elasticity and traction to rupture were measured using Universal test equipment. Scanning electron microscopy (SEM) was used to measure the hair surface morphological conditions. A large majority of the volunteers indicated that the beginning of greying hair can negatively influence their quality of life. They also reported that the primary changes of their hair were the frizziness, dryness and loss of shine, increased loss and breakage and thinning. Morphological evaluation showed that the non-pigmented hair cuticles were thinner than pigmented hair. The adherence of the cuticle scales was more pronounced in the non-pigmented hair .Finally, this study showed the influence of the appearance of grey hair on quality of life in women as well as the morphological alterations resulting from the greying processes
FERM Domain Interaction Promotes FAK Signaling
From the results of deletion analyses, the FERM domain of FAK has been proposed to inhibit enzymatic activity and repress FAK signaling. We have identified a sequence in the FERM domain that is important for FAK signaling in vivo. Point mutations in this sequence had little effect upon catalytic activity in vitro. However, the mutant exhibits reduced tyrosine phosphorylation and dramatically reduced Src family kinase binding. Further, the abilities of the mutant to transduce biochemical signals and to promote cell migration were severely impaired. The results implicate a FERM domain interaction in cell adhesion-dependent activation of FAK and downstream signaling. We also show that the purified FERM domain of FAK interacts with full-length FAK in vitro, and mutation of this sequence disrupts the interaction. These findings are discussed in the context of models of FAK regulation by its FERM domain
Euclid preparation. XXX. Performance assessment of the NISP red grism through spectroscopic simulations for the wide and deep surveys
This work focusses on the pilot run of a simulation campaign aimed at investigating the spectroscopic capabilities of the Euclid Near-Infrared Spectrometer and Photometer (NISP), in terms of continuum and emission line detection in the context of galaxy evolutionary studies. To this purpose, we constructed, emulated, and analysed the spectra of 4992 star-forming galaxies at 0.3 †z †2.5 using the NISP pixel-level simulator. We built the spectral library starting from public multi-wavelength galaxy catalogues, with value-added information on spectral energy distribution (SED) fitting results, and stellar population templates from Bruzual & Charlot (2003, MNRAS, 344, 1000). Rest-frame optical and near-IR nebular emission lines were included using empirical and theoretical relations. Dust attenuation was treated using the Calzetti extinction law accounting for the differential attenuation in line-emitting regions with respect to the stellar continuum. The NISP simulator was configured including instrumental and astrophysical sources of noise such as the dark current, read-out noise, zodiacal background, and out-of-field stray light. In this preliminary study, we avoided contamination due to the overlap of the slitless spectra. For this purpose, we located the galaxies on a grid and simulated only the first order spectra. We inferred the 3.5Ï NISP red grism spectroscopic detection limit of the continuum measured in the H band for star-forming galaxies with a median disk half-light radius of 0.âł4 at magnitude H = 19.5 ± 0.2 AB mag for the Euclid Wide Survey and at H = 20.8 ± 0.6 AB mag for the Euclid Deep Survey. We found a very good agreement with the red grism emission line detection limit requirement for the Wide and Deep surveys. We characterised the effect of the galaxy shape on the detection capability of the red grism and highlighted the degradation of the quality of the extracted spectra as the disk size increased. In particular, we found that the extracted emission line signal-to-noise ratio (S/N) drops by ~45% when the disk size ranges from 0.âł25 to 1âł. These trends lead to a correlation between the emission line S/N and the stellar mass of the galaxy and we demonstrate the effect in a stacking analysis unveiling emission lines otherwise too faint to detect
High expression of focal adhesion kinase (p125(FAK)) in node-negative breast cancer is related to overexpression of HER-2/neu and activated Akt kinase but does not predict outcome
INTRODUCTION: Focal adhesion kinase (FAK) regulates multiple cellular processes including growth, differentiation, adhesion, motility and apoptosis. In breast carcinoma, FAK overexpression has been linked to cancer progression but the prognostic relevance remains unknown. In particular, with regard to lymph node-negative breast cancer it is important to identify high-risk patients who would benefit from further adjuvant therapy. METHODS: We analyzed 162 node-negative breast cancer cases to determine the prognostic relevance of FAK expression, and we investigated the relationship of FAK with major associated signaling pathways (HER2, Src, Akt and extracellular regulated kinases) by immunohistochemistry and western blot analysis. RESULTS: Elevated FAK expression did not predict patient outcome, in contrast to tumor grading (P = 0.005), Akt activation (P = 0.0383) and estrogen receptor status (P = 0.0033). Significant positive correlations were observed between elevated FAK expression and HER2 overexpression (P = 0.001), as well as phospho-Src Tyr-215 (P = 0.021) and phospho-Akt (P < 0.001), but not with phospho-ERK1/2 (P = 0.108). Western blot analysis showed a significant correlation of FAK Tyr-861 activation and HER2 overexpression (P = 0.01). CONCLUSIONS: Immunohistochemical detection of FAK expression is of no prognostic significance in node-negative breast cancer but provides evidence that HER2 is involved in tumor malignancy and metastatic ability of breast cancer through a novel signaling pathway participating FAK and Src
FAK alters invadopodia and focal adhesion composition and dynamics to regulate breast cancer invasion
Focal adhesion kinase (FAK) is important for breast cancer progression and invasion and is necessary for the dynamic turnover of focal adhesions. However, it has not been determined whether FAK also regulates the dynamics of invasive adhesions formed in cancer cells known as invadopodia. In this study, we report that endogenous FAK functions upstream of cellular Src (c-Src) as a negative regulator of invadopodia formation and dynamics in breast cancer cells. We show that depletion of FAK induces the formation of active invadopodia but impairs invasive cell migration. FAK-deficient MTLn3 breast cancer cells display enhanced assembly and dynamics of invadopodia that are rescued by expression of wild-type FAK but not by FAK that cannot be phosphorylated at tyrosine 397. Moreover, our findings demonstrate that FAK depletion switches phosphotyrosine-containing proteins from focal adhesions to invadopodia through the temporal and spatial regulation of c-Src activity. Collectively, our findings provide novel insight into the interplay between FAK and Src to promote invasion
Euclid preparation: XXX. Performance assessment of the NISP red grism through spectroscopic simulations for the wide and deep surveys
This work focusses on the pilot run of a simulation campaign aimed at investigating the spectroscopic capabilities of the Euclid Near-Infrared Spectrometer and Photometer (NISP), in terms of continuum and emission line detection in the context of galaxy evolutionary studies. To this purpose, we constructed, emulated, and analysed the spectra of 4992 star-forming galaxies at 0:3 â„ z â„ 2:5 using the NISP pixel-level simulator. We built the spectral library starting from public multi-wavelength galaxy catalogues, with value-added information on spectral energy distribution (SED) fitting results, and stellar population templates from Bruzual & Charlot (2003, MNRAS, 344, 1000). Rest-frame optical and near-IR nebular emission lines were included using empirical and theoretical relations. Dust attenuation was treated using the Calzetti extinction law accounting for the differential attenuation in line-emitting regions with respect to the stellar continuum. The NISP simulator was configured including instrumental and astrophysical sources of noise such as the dark current, read-out noise, zodiacal background, and out-of-field stray light. In this preliminary study, we avoided contamination due to the overlap of the slitless spectra. For this purpose, we located the galaxies on a grid and simulated only the first order spectra.We inferred the 3.5ÎŽ NISP red grism spectroscopic detection limit of the continuum measured in the H band for star-forming galaxies with a median disk half-light radius of 0: 004 at magnitude H = 19:5 = 0:2ABmag for the Euclid Wide Survey and at H = 20:8 = 0:6ABmag for the Euclid Deep Survey. We found a very good agreement with the red grism emission line detection limit requirement for the Wide and Deep surveys. We characterised the effect of the galaxy shape on the detection capability of the red grism and highlighted the degradation of the quality of the extracted spectra as the disk size increased. In particular, we found that the extracted emission line signal-to-noise ratio (S/N) drops by 45% when the disk size ranges from 0: 0025 to 100. These trends lead to a correlation between the emission line S/N and the stellar mass of the galaxy and we demonstrate the effect in a stacking analysis unveiling emission lines otherwise too faint to detect
Carcinoma Matrix Controls Resistance to Cisplatin through Talin Regulation of NF-kB
Extracellular matrix factors within the tumor microenvironment that control resistance to chemotherapeutics are poorly understood. This study focused on understanding matrix adhesion pathways that control the oral carcinoma response to cisplatin. Our studies revealed that adhesion of HN12 and JHU012 oral carcinomas to carcinoma matrix supported tumor cell proliferation in response to treatment with cisplatin. Proliferation in response to 30 ”M cisplatin was not observed in HN12 cells adherent to other purified extracellular matrices such as Matrigel, collagen I, fibronectin or laminin I. Integrin ÎČ1 was important for adhesion to carcinoma matrix to trigger proliferation after treatment with cisplatin. Disruption of talin expression in HN12 cells adherent to carcinoma matrix increased cisplatin induced proliferation. Pharmacological inhibitors were used to determine signaling events required for talin deficiency to regulate cisplatin induced proliferation. Pharmacological inhibition of NF-kB reduced proliferation of talin-deficient HN12 cells treated with 30 ”M cisplatin. Nuclear NF-kB activity was assayed in HN12 cells using a luciferase reporter of NF-kB transcriptional activity. Nuclear NF-kB activity was similar in HN12 cells adherent to carcinoma matrix and collagen I when treated with vehicle DMSO. Following treatment with 30 ”M cisplatin, NF-kB activity is maintained in cells adherent to carcinoma matrix whereas NF-kB activity is reduced in collagen I adherent cells. Expression of talin was sufficient to trigger proliferation of HN12 cells adherent to collagen I following treatment with 1 and 30 ”M cisplatin. Talin overexpression was sufficient to trigger NF-kB activity following treatment with cisplatin in carcinoma matrix adherent HN12 cells in a process disrupted by FAK siRNA. Thus, adhesions within the carcinoma matrix create a matrix environment in which exposure to cisplatin induces proliferation through the function of integrin ÎČ1, talin and FAK pathways that regulate NF-kB nuclear activity
Euclid preparation. TBD. The effect of linear redshift-space distortions in photometric galaxy clustering and its cross-correlation with cosmic shear
Cosmological surveys planned for the current decade will provide us with
unparalleled observations of the distribution of galaxies on cosmic scales, by
means of which we can probe the underlying large-scale structure (LSS) of the
Universe. This will allow us to test the concordance cosmological model and its
extensions. However, precision pushes us to high levels of accuracy in the
theoretical modelling of the LSS observables, in order not to introduce biases
in the estimation of cosmological parameters. In particular, effects such as
redshift-space distortions (RSD) can become relevant in the computation of
harmonic-space power spectra even for the clustering of the photometrically
selected galaxies, as it has been previously shown in literature studies. In
this work, we investigate the contribution of linear RSD, as formulated in the
Limber approximation by arXiv:1902.07226, in forecast cosmological analyses
with the photometric galaxy sample of the Euclid survey, in order to assess
their impact and quantify the bias on the measurement of cosmological
parameters that neglecting such an effect would cause. We perform this task by
producing mock power spectra for photometric galaxy clustering and weak
lensing, as expected to be obtained from the Euclid survey. We then use a
Markov chain Monte Carlo approach to obtain the posterior distributions of
cosmological parameters from such simulated observations. We find that
neglecting the linear RSD leads to significant biases both when using galaxy
correlations alone and when these are combined with cosmic shear, in the
so-called 32pt approach. Such biases can be as large as
-equivalent when assuming an underlying CDM cosmology. When
extending the cosmological model to include the equation-of-state parameters of
dark energy, we find that the extension parameters can be shifted by more than
.Comment: 15 pages, 5 figures. To be submitted in A&
Euclid preparation. XXXI. Performance assessment of the NISP Red-Grism through spectroscopic simulations for the Wide and Deep surveys
This work focuses on the pilot run of a simulation campaign aimed at
investigating the spectroscopic capabilities of the Euclid Near-Infrared
Spectrometer and Photometer (NISP), in terms of continuum and emission line
detection in the context of galaxy evolutionary studies. To this purpose we
constructed, emulated, and analysed the spectra of 4992 star-forming galaxies
at using the NISP pixel-level simulator. We built the
spectral library starting from public multi-wavelength galaxy catalogues, with
value-added information on spectral energy distribution (SED) fitting results,
and from Bruzual and Charlot (2003) stellar population templates. Rest-frame
optical and near-IR nebular emission lines were included using empirical and
theoretical relations. We inferred the 3.5 NISP red grism spectroscopic
detection limit of the continuum measured in the band for star-forming
galaxies with a median disk half-light radius of \ang{;;0.4} at magnitude ABmag for the Euclid Wide Survey and at ABmag for the Euclid Deep Survey. We found a very good
agreement with the red grism emission line detection limit requirement for the
Wide and Deep surveys. We characterised the effect of the galaxy shape on the
detection capability of the red grism and highlighted the degradation of the
quality of the extracted spectra as the disk size increases. In particular, we
found that the extracted emission line signal to noise ratio (SNR) drops by
45 when the disk size ranges from \ang{;;0.25} to \ang{;;1}. These
trends lead to a correlation between the emission line SNR and the stellar mass
of the galaxy and we demonstrate the effect in a stacking analysis unveiling
emission lines otherwise too faint to detect.Comment: 23 pages, 21 figure
Euclid preparation. XXIX. Water ice in spacecraft part I: The physics of ice formation and contamination
Molecular contamination is a well-known problem in space flight. Water is the
most common contaminant and alters numerous properties of a cryogenic optical
system. Too much ice means that Euclid's calibration requirements and science
goals cannot be met. Euclid must then be thermally decontaminated, a long and
risky process. We need to understand how iced optics affect the data and when a
decontamination is required. This is essential to build adequate calibration
and survey plans, yet a comprehensive analysis in the context of an
astrophysical space survey has not been done before.
In this paper we look at other spacecraft with well-documented outgassing
records, and we review the formation of thin ice films. A mix of amorphous and
crystalline ices is expected for Euclid. Their surface topography depends on
the competing energetic needs of the substrate-water and the water-water
interfaces, and is hard to predict with current theories. We illustrate that
with scanning-tunnelling and atomic-force microscope images.
Industrial tools exist to estimate contamination, and we must understand
their uncertainties. We find considerable knowledge errors on the diffusion and
sublimation coefficients, limiting the accuracy of these tools. We developed a
water transport model to compute contamination rates in Euclid, and find
general agreement with industry estimates. Tests of the Euclid flight hardware
in space simulators did not pick up contamination signals; our in-flight
calibrations observations will be much more sensitive.
We must understand the link between the amount of ice on the optics and its
effect on Euclid's data. Little research is available about this link, possibly
because other spacecraft can decontaminate easily, quenching the need for a
deeper understanding. In our second paper we quantify the various effects of
iced optics on spectrophotometric data.Comment: 35 pages, 22 figures, A&A in press. Changes to previous version:
language edits, added Z. Bolag as author in the arxiv PDF (was listed in the
ASCII author list and in the journal PDF, but not in the arxiv PDF). This
version is identical to the journal versio
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