4,963 research outputs found

    Sensitivity of an Ultrasonic Technique for Axial Stress Determination

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    In machine assembly it is often required that bolts used to fasten machine parts be installed with specific design preloads. Because it is inconvenient to measure preload directly, preload specifications are usually based on some more easily measured quantity with which the level of preload may be correlated. Most often this quantity is the torque to be applied to the bolt at installation. Studies by Blake and Kurtz [1] and Heyman [2] have shown that when bolts are torqued into place, the fraction of applied torque which translates into useful preload is small and widely variable. This is so because the large majority of applied torque is absorbed in overcoming friction in the bolt’s threads and at the underside of the bolt’s head. Consequently, even though the torque to install different bolts may be identical, small variations in frictional conditions from one installation to the next can result in large variations in preload. The unreliability of torque as an indicator of preload has been the motivating factor behind the development of a number of alternate methods of measurement [2–5]

    Measurement of Thermal Stress in Railroad Rails Using Ultrasonic SH Waves

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    The use of welded joints in railroad tracks has led to problems of rail buckling brought about by the development of large compressive stresses during hot days. On cold days, tensile stresses can actually fracture the rail. In order to prevent this source of derailments, it is desirable to develop an easily used instrument to measure the level of stress in an arbitrary section of track in the field. Ultrasonic birefringence, acoustic emission and certain magnetic phenomena have all been used to attack this problem but they all suffer from the necessity for calibrating the sensor under stress-free conditions in order to correct for metallurgical structure variations. A new ultrasonic technique based on using surface skimming shear horizontal ultrasonic waves generated and detected by EMATs was investigated here because it rigorously eliminates the effects of metallurgical texture as well as unreliable coupling of the transducer to the part. Tests on sections of rail mounted in a 200,000 pound testing machine at the University of New Mexico demonstrated that the theory for the basic phenomenon is correct and that the stress level can be measured in spite of the presence of considerable texture in the rail microstructure

    Acoustoelastic Measurement of Second- and Third-Order Elastic Constants in Silicon Carbide and Alumina Particulate-Reinforced Aluminum Metal Matrix Composites

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    Metal Matrix Composites (MMCs) can offer increased specific strength and stiffness, increased toughness, and an ability to operate at high temperatures. However, the mechanical properties of MMCs can be greatly influenced by residual stresses that result during fabrication from the differences in coefficients of thermal expansion of the constituents [1]. There are a number of non-destructive techniques for measuring residual stresses in these types of composites. The two most widely used techniques are x-ray and neutron diffraction. The x-ray diffraction technique is limited in that it has small penetration depths and beam sizes which are generally much greater than the reinforcement diameter. Although the neutron diffraction technique can provide through-thickness measurements, the availability of the specialized test equipment is a limitation, due to the necessity for using a neutron source [2]. An alternative technique for measuring residual stresses is using ultrasound

    Acoustoelastic Wave Velocity in Metal Matrix Composite under Thermal Loading

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    It is well known that microstresses are developed in a composite subjected to a temperature change due to the mismatch in thermal expansion between the fibers and the matrix. The stresses in the matrix can be large enough to cause the matrix to yield and deform plastically. The nonlinear thermal behavior is evidenced by experimentally observed thermal hysteresis in a metal matrix composite under thermal cycling [1]. Obviously, the thermal hysteresis plays an important role on the dimensional stability of the metal matrix composites, especially for graphite fiber reinforced composites

    Dimeric structures of quinol-dependent nitric oxide reductases (qNORs) revealed by cryo–electron microscopy

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    Quinol-dependent nitric oxide reductases (qNORs) are membrane-integrated, iron-containing enzymes of the denitrification pathway, which catalyze the reduction of nitric oxide (NO) to the major ozone destroying gas nitrous oxide (N2O). Cryo–electron microscopy structures of active qNOR from Alcaligenes xylosoxidans and an activity-enhancing mutant have been determined to be at local resolutions of 3.7 and 3.2 Å, respectively. They unexpectedly reveal a dimeric conformation (also confirmed for qNOR from Neisseria meningitidis) and define the active-site configuration, with a clear water channel from the cytoplasm. Structure-based mutagenesis has identified key residues involved in proton transport and substrate delivery to the active site of qNORs. The proton supply direction differs from cytochrome c–dependent NOR (cNOR), where water molecules from the cytoplasm serve as a proton source similar to those from cytochrome c oxidase

    Graphene-VP40 interactions and potential disruption of the Ebola virus matrix filaments

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    Ebola virus infections cause hemorrhagic fever that often results in very high fatality rates. In addition to exploring vaccines, development of drugs is also essential for treating the disease and preventing the spread of the infection. The Ebola virus matrix protein VP40 exists in various conformational and oligomeric forms and is a potential pharmacological target for disrupting the virus life-cycle. Here we explored graphene-VP40 interactions using molecular dynamics simulations and graphene pelleting assays. We found that graphene sheets associate strongly with VP40 at various interfaces. We also found that the graphene is able to disrupt the C-terminal domain (CTD-CTD) interface of VP40 hexamers. This VP40 hexamer-hexamer interface is crucial in forming the Ebola viral matrix and disruption of this interface may provide a method to use graphene or similar nanoparticle based solutions as a disinfectant that can significantly reduce the spread of the disease and prevent an Ebola epidemic

    Issues in the High Resolution Acoustoelastic Measurement of Stress

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    The acoustoelastic measurement of stress is a topic with a rich history and the basic principles are well known [1]. In summary, one takes advantage of various nonlinearities which govern the elastic response of a solid, including but not limited to anharmonicities in interatomic forces, which lead to a stress dependence of the ultrasonic velocity. The basic idea, then is to precisely measure the velocity and to infer stress from a relation of the form V=Vo+Kσ where V is the measured velocity in the presence of a stress σ, Vo is the value that would have been observed in the absence of that stress, and K is known as the acoustoelastic constant.</p

    Explicit Formulae Showing the Effects of Texture on Acoustoelastic Coefficients

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    It is well known that crystallographic texture not only modifies the elastic constants of polycrystalline aggregates at (unstressed) natural states but also affects their acoustoelastic coefficients when the aggregates are stressed. While exact knowledge about the effects of texture on acoustoelastic coefficients has hitherto remained wanting, such effects are usually assumed to be negligible and are ignored in practical applications of acoustoelasticity (cf. [1] for example). Concerning this common practice, Thompson et al. [2] have urged caution: Care must be taken when [this] assumption is made since the influence of texture on acoustoelastic constants is stronger than its influence on elastic moduli or velocities

    Embryological staging of the Zebra Finch, Taeniopygia guttata

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    Zebra Finches (Taeniopygia guttata) are the most commonly used laboratory songbird species, yet their embryological development has been poorly characterized. Most studies to date apply Hamburger and Hamilton stages derived from chicken development; however, significant differences in development between precocial and altricial species suggest that they may not be directly comparable. We provide the first detailed description of embryological development in the Zebra Finch under standard artificial incubation. These descriptions confirm that some of the features used to classify chicken embryos into stages are not applicable in an altricial bird such as the Zebra Finch. This staging protocol will help to standardize future studies of embryological development in the Zebra Finch. J. Morphol. 274:1090-1110, 2013. (c) 2013 Wiley Periodicals, Inc

    An Ultrasonic Study on Anelasticity in Metals

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    Ultrasonic waves are highly sensitive to microstructural variations in materials and have been used extensively to investigate anharmonic effects in various metals and alloys[1–3]. A major focus of these studies is on the higher order elastic constants and their relation to the microstructure of the material. Ultrasonic techniques have also proven quite useful for characterizing the stress state of a material [4–6]. Recently, while using the magnetoacoustic (MAC) method to investigate the residual stress in various steel samples, a time dependent change in the results was observed. It became apparent that the measurements were exhibiting anelastic effects due to some intrinsic properties of the samples.</p
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