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Impacts of parameterized orographic drag on the Northern Hemisphere winter circulation
A recent intercomparison exercise proposed by the Working Group for Numerical Experimentation
(WGNE) revealed that the parameterized, or unresolved, surface stress in weather forecast models is
highly model-dependent, especially over orography. Models of comparable resolution differ over land by as
much as 20% in zonal mean total subgrid surface stress (Ttot). The way Ttot is partitioned between the different
parameterizations is also model-dependent. In this study, we simulated in a particular model an increase in
Ttot comparable with the spread found in the WGNE intercomparison. This increase was simulated in two
ways, namely by increasing independently the contributions to Ttot of the turbulent orographic form drag
scheme (TOFD) and of the orographic low-level blocking scheme (BLOCK). Increasing the parameterized orographic
drag leads to significant changes in surface pressure, zonal wind and temperature in the Northern
Hemisphere during winter both in 10 day weather forecasts and in seasonal integrations. However, the magnitude
of these changes in circulation strongly depends on which scheme is modified. In 10 day forecasts, stronger
changes are found when the TOFD stress is increased, while on seasonal time scales the effects are of
comparable magnitude, although different in detail. At these time scales, the BLOCK scheme affects the lower
stratosphere winds through changes in the resolved planetary waves which are associated with surface
impacts, while the TOFD effects are mostly limited to the lower troposphere. The partitioning of Ttot between
the two schemes appears to play an important role at all time scales
Análise faunística de cochonilhas (hemiptera: sternorrhyncha) na cultura da videira no município de Pinto Bandeira, Rio Grande do Sul, Brasil.
O Rio Grande do Sul é o principal estado produtor de uvas e vinhos do país, com destaque para a região da Serra Gaúcha. Um dos principais problemas enfrentados pelos viticultores é a presença de cochonilhas que ocorrem na parte aérea da cultura e que, dependendo da severidade dos danos causam a morte da planta. Nesse trabalho foi realizada uma análise faunística das cochonilhas presentes na parte aérea de videiras, no município de Pinto Bandeira, RS. As amostragens foram realizadas quinzenalmente em vinhedo comercial de 1 ha de Vitis vinifera L. cultivar Merlot. Foram coletadas amostras de cochonilhas presentes no ritidoma e brotações, de janeiro de 2011 a janeiro de 2013. A triagem e identificação dos espécimes foram realizadas em laboratório com o auxílio de microscópio óptico e bibliografia especializada. Os dados obtidos foram analisados a partir da determinação da frequência, constância e dominância. Foram identificadas duas espécies de cochonilhas incluídas em Diaspididae - Hemiberlesia latanie e Pseudaonidia marquesi; e uma em Monophlebidae - Icerya schrottkyi. Hemiberlesia latanie foi a espécie que apresentou 89,72% de frequência, I. schrottkyi 7,13% e P. marquesi 3,14%. Hemiberlesia latanie foi a cochonilha dominante, mais frequente e constante no vinhedo
Impacts of orography on large-scale atmospheric circulation
Some of the largest and most persistent circulation errors in global numerical weather prediction and climate models are attributable to the inadequate representation of the impacts of orography on the atmospheric flow. Existing parametrization approaches attempting to account for unresolved orographic processes, such as turbulent form drag, low-level flow blocking or mountain waves, have been successful to some extent. They capture the basic impacts of the unresolved orography on atmospheric circulation in a qualitatively correct way and have led to significant progress in both numerical weather prediction and climate modelling. These approaches, however, have apparent limitations and inadequacies due to poor observational evidence, insufficient fundamental knowledge and an ambiguous separation between resolved and unresolved orographic scales and between different orographic processes. Numerical weather prediction and climate modelling has advanced to a stage where these inadequacies have become critical and hamper progress by limiting predictive skill on a wide range of spatial and temporal scales. More physically-based approaches are needed to quantify the relative importance of apparently disparate orographic processes and to account for their combined effects in a rational and accurate way in numerical models. We argue that, thanks to recent advances, significant progress can be made by combining theoretical approaches with observations, inverse modelling techniques and high-resolution and idealized numerical simulations
AKT1 and MYC induce distinctive metabolic fingerprints in human prostate cancer
Cancer cells may overcome growth factor dependence by deregulating oncogenic and/or tumor-suppressor pathways that affect their metabolism, or by activating metabolic pathways de novo with targeted mutations in critical metabolic enzymes. It is unknown whether human prostate tumors develop a similar metabolic response to different oncogenic drivers or a particular oncogenic event results in its own metabolic reprogramming. Akt and Myc are arguably the most prevalent driving oncogenes in prostate cancer. Mass spectrometry-based metabolite profiling was performed on immortalized human prostate epithelial cells transformed by AKT1 or MYC, transgenic mice driven by the same oncogenes under the control of a prostate-specific promoter, and human prostate specimens characterized for the expression and activation of these oncoproteins. Integrative analysis of these metabolomic datasets revealed that AKT1 activation was associated with accumulation of aerobic glycolysis metabolites, whereas MYC overexpression was associated with dysregulated lipid metabolism. Selected metabolites that differentially accumulated in the MYC-high versus AKT1-high tumors, or in normal versus tumor prostate tissue by untargeted metabolomics, were validated using absolute quantitation assays. Importantly, the AKT1/MYC status was independent of Gleason grade and pathologic staging. Our fi ndings show how prostate tumors undergo a metabolic reprogramming that refl ects their molecular phenotypes, with implications for the development of metabolic diagnostics and targeted therapeutics.Instituto de Investigaciones Bioquímicas de La PlataFacultad de Ciencias Médica
Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe
The picornavirus named ‘Ljungan virus’ (LV, species Parechovirus B) has been detected in a dozen small mammal species from across Europe, but detailed information on its genetic diversity and host specificity is lacking. Here, we analyze the evolutionary relationships of LV variants circulating in free-living mammal populations by comparing the phylogenetics of the VP1 region (encoding the capsid protein and associated with LV serotype) and the 3Dpol region (encoding the RNA polymerase) from 24 LV RNA-positive animals and a fragment of the 5′ untranslated region (UTR) sequence (used for defining strains) in sympatric small mammals. We define three new VP1 genotypes: two in bank voles (Myodes glareolus) (genotype 8 from Finland, Sweden, France, and Italy, and genotype 9 from France and Italy) and one in field voles (Microtus arvalis) (genotype 7 from Finland). There are several other indications that LV variants are host-specific, at least in parts of their range. Our results suggest that LV evolution is rapid, ongoing and affected by genetic drift, purifying selection, spillover and host evolutionary history. Although recent studies suggest that LV does not have zoonotic potential, its widespread geographical and host distribution in natural populations of well-characterized small mammals could make it useful as a model for studying RNA virus evolution and transmission
Evolutionary Relationships of Ljungan Virus Variants Circulating in Multi-Host Systems across Europe
The picornavirus named ‘Ljungan virus’ (LV, species Parechovirus B) has been detected in a dozen small mammal species from across Europe, but detailed information on its genetic diversity and host specificity is lacking. Here, we analyze the evolutionary relationships of LV variants circulating in free-living mammal populations by comparing the phylogenetics of the VP1 region (encoding the capsid protein and associated with LV serotype) and the 3Dpol region (encoding the RNA polymerase) from 24 LV RNA-positive animals and a fragment of the 5′ untranslated region (UTR) sequence (used for defining strains) in sympatric small mammals. We define three new VP1 genotypes: two in bank voles (Myodes glareolus) (genotype 8 from Finland, Sweden, France, and Italy, and genotype 9 from France and Italy) and one in field voles (Microtus arvalis) (genotype 7 from Finland). There are several other indications that LV variants are host-specific, at least in parts of their range. Our results suggest that LV evolution is rapid, ongoing and affected by genetic drift, purifying selection, spillover and host evolutionary history. Although recent studies suggest that LV does not have zoonotic potential, its widespread geographical and host distribution in natural populations of well-characterized small mammals could make it useful as a model for studying RNA virus evolution and transmission
Molecular genetic analysis of severe coagulation factor XI deficiency in six Italian patients
BACKGROUND AND OBJECTIVES: Factor XI (FXI) deficiency is a rare autosomal recessive coagulopathy which is, however, frequent among Ashkenazi Jews. Two mutations, type II (Glu117stop) and type III (Phe283Leu), account for the majority of abnormal alleles in this population. The aim of this study was to analyze the molecular basis of FXI deficiency in six unrelated Italian probands with severe deficiency, a population hitherto largely unexplored. DESIGN AND METHODS: All patients showed unmeasurable functional FXI levels in plasma. Mutational screening was performed by sequencing. Haplotype analysis was performed using intragenic polymorphisms. Expression studies were performed by transient transfection in COS-1 cells. RESULTS: Sequencing identified two novel mutations: a nonsense mutation (Cys118stop) in exon 5 in two probands, and a 6-bp deletion (643-648delATCGAC) in exon 7 in one proband. The Cys118stop is predicted to cause FXI deficiency by a secretion defect and/or by increased mRNA degradation. The 6-bp deletion causes the loss of residues Ile197 and Asp198. There was a remarkable secretion impairment of the deleted FXI protein. In four of the six probands, the type II mutation was found. Haplotype analysis in patients carrying the type II mutation revealed that they share a common haplotype, perhaps derived from a Jewish ancestor. INTERPRETATION AND CONCLUSIONS: The identification and characterization of two novel mutations widen the mutational spectrum of FXI deficiency. Haplotype analysis is compatible with a Jewish origin of the type II mutation. The high occurrence of the type II mutation among Italian patients will be helpful to direct future genetic screenings
Shallow whole-genome sequencing of Aedes japonicus and Aedes koreicus from Italy and an updated picture of their evolution based on mitogenomics and barcoding
Aedes japonicus and Aedes koreicus are two invasive mosquitoes native to East Asia that are quickly establishing in temperate regions of Europe. Both species are vectors of arboviruses, but we currently lack a clear understanding of their evolution. Here, we present new short-read, shallow genome sequencing of A. japonicus and A. koreicus individuals from northern Italy, which we used for downstream phylogenetic and barcode analyses. We explored associated microbial DNA and found high occurrences of Delftia bacteria in both samples, but neither Asaia nor Wolbachia. We then assembled complete mitogenomes and used these data to infer divergence times estimating the split of A. japonicus from A. koreicus in the Oligocene, which was more recent than that previously reported using mitochondrial markers. We recover a younger age for most other nodes within Aedini and other Culicidae. COI barcoding and phylogenetic analyses indicate that A. japonicus yaeyamensis, A. japonicus amamiensis, and the two A. koreicus sampled from Europe should be considered as separate species within a monophyletic species complex. Our studies further clarify the evolution of A. japonicus and A. koreicus, and indicate the need to obtain whole-genome data from putative species in order to disentangle their complex patterns of evolution
On a Testing Methodology for the Mechanical Property Assessment of a New Low-Cost Titanium Alloy Derived from Synthetic Rutile
Mechanical property data of a low-cost titanium alloy derived directly from synthetic rutile is reported. A small-scale testing approach comprising consolidation via field-assisted sintering technology, followed by axisymmetric compression testing, has been designed to yield mechanical property data from small quantities of titanium alloy powder. To validate this approach and provide a benchmark, Ti-6Al-4V powder has been processed using the same methodology and compared with material property data generated from thermo-physical simulation software. Compressive yield strength and strain to failure of the synthetic rutile-derived titanium alloy were revealed to be similar to that of Ti-6Al-4V
Economic values for milk production and quality traits of Guzerá cattle in Brazil.
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