Histopathological and immunohistochemical differentiation of epithelial alterations in vocal nodule comparing to polyps and to laryngeal edema

AIM: To evaluate by histological and immunohistochemical methods the epithelial lesions in vocal nodule and correlate with polyp, laryngeal edema and vocal folds without macroscopic lesions. STUDY DESIGN: Chart review. MATERIAL AND METHOD: In a retrospective analysis of medical records, twenty-six patients, who underwent microsurgical excision of laryngeal inflammatory lesions (nodules, polyps and laryngeal edema), were identified. Vocal folds without macroscopic lesions were obtained from autopsy. To evaluate epithelial lesions, specimens were stained with H&E, PAS, and with antibodies against laminin and collagen type IV. These specimens were analyzed by light microscopy. RESULTS: Lesions diagnosed as nodules showed more collagen type IV and laminin deposition when compared to polyps (p=0,034 and p=0,036, respectively) and to vocal folds without macroscopic lesions (p=0,019 and p=0,021, respectively). Nodules showed basement membranes thicker than polyps on PAS stain (p=0,102). Laryngeal edema could not be differentiated from nodules, polyps or vocal folds without macroscopic lesions by PAS, laminin or collagen type IV stain (p>0,10). CONCLUSION: By histological and immunohistochemical techniques to evaluate epithelium of laryngeal inflammatory lesions we conclude that vocal fold nodule differentiate from polyps on the three techniques used to stain basement membrane (PAS, laminin, collagen IV) and from vocal folds without macroscopic lesions on two techniques used (laminin, collagen IV). Laryngeal edema cannot be differentiated from the others laryngeal lesions, neither from vocal folds without macroscopic lesions, when the techniques before described are used.OBJETIVO: Avaliar, por meio de técnicas histológicas e imunoistoquímicas, o epitélio nos nódulos vocais em relação aos pólipos, ao edema de laringe e às pregas vocais sem lesões macroscópicas. FORMA DE ESTUDO: Clínico retrospectivo. MATERIAL E MÉTODO: Por meio de levantamento de prontuário, foram identificados vinte e seis pacientes com lesões laríngeas inflamatórias (nódulos, pólipos e edema de laringe), que haviam sido submetidos à cirurgia. Pregas vocais sem alterações macroscópicas foram obtidas a partir de autópsia de cadáveres. Para análise do epitélio, foram realizadas colorações pela técnica da hematoxilina-eosina e do ácido periódico de Schiff e técnicas de imunoistoquímica com anticorpos dirigidos contra laminina e colágeno IV. A leitura das lâminas foi realizada por meio de microscopia óptica. RESULTADOS: Houve maior imunoexpressão de laminina e colágeno IV nos nódulos quando comparados aos pólipos (p=0,034 e p=0, 036, respectivamente), assim como quando comparados às pregas vocais sem lesões macroscópicas (p=0,019 e p=0, 021, respectivamente). Nódulos tendem a apresentar maior espessura da membrana basal, quando utilizamos coloração pela técnica do PAS, em relação aos pólipos (p=0,102). O edema de laringe não se diferenciou das demais nas técnicas utilizadas (p> 0,10). CONCLUSÕES: O Nódulo Vocal diferencia-se dos pólipos, nas três técnicas utilizadas para detecção da membrana basal (PAS, laminina, colágeno IV), e das pregas vocais sem lesões macroscópicas em duas das técnicas utilizadas (laminina e colágeno IV). Edema de laringe não se diferencia das demais lesões, nem de pregas vocais sem lesões macroscópicas, quando utilizadas as técnicas anteriormente descritas, para estudo da membrana basal.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Otorrinolaringologia e Distúrbios da Comunicação HumanaUniversidade de São Paulo Faculdade de OdontologiaUNIFESP, EPM, Depto. de Otorrinolaringologia e Distúrbios da Comunicação HumanaSciEL

Competitive adsorption of wellbores corrosion inhibitor onto steel, esmectite and sandstone

A commercial corrosion inhibitor used in petroleum production was characterized by means of infrared spectroscopy and energy dispersive spectroscopy (EDS). Predicting the adsorption behavior of corrosion inhibitor onto steel, sandstone and esmectite is the key to improve working conditions. In this study, the adsorption kinetics of inhibitor formulations in HCl 15% or in Mud Acid (HCl 13,5% and ammonium bifluoride) onto steel, sandstone and esmectite was determined by means of spectrophotometry. Kinetic parameters indicated that adsorption of inhibitor in the presence of bifluoride was favored. Moreover, the adsorption constant rate was the largest when the substrate was esmectite.FAPESPCNP

From Closed-Loop to Sustainable Supply Chains: The WEEE case

The primary objective of closed-loop supply chains (CLSC) is to reap the maximum economic benefit from end-of-use products. Nevertheless, literature within this stream of research advocates that closing the loop helps to mitigate the undesirable footprint of supply chains. In this paper we assess the magnitude of such environmental gains for Electric and Electronic Equipments (EEE), based on a single environmental metric of Cumulative Energy Demand. We detail our analysis for the different phases of the CLSC, i.e. manufacturing, usage, transportation and end-of-life activities. According to our literature review, within the same group of EEE, results greatly vary. Furthermore, based on the environmental hot-spots, we propose extensions of the existing CLSC models to incorporate the CED

Alterations on the evapotranspiration of sugarcane cultivars under distinct salinity levels applied in the fertigation

The aim of this research was to evaluate the effect of distinct levels of salinity on the evapotranspiration of the RB867515, RB855453, RB92579 and RB928064 sugarcane cultivars. The evapotranspiration was monitored during daytime period under meteorological conditions influenced, mainly by cloud variations. The salinity values were established by addition of 0, 50, 100 and 150 mM of Sodium Chloride (NaCl) to the nutrient solution, which was applied by fertigation, in such way that the electrical conductivity (EC) of the leached solution was near 3, 6, 10 and 13 dS m -1 , respectively. The increase of the NaCl concentration in the nutrient solution affected, significantly and in a linear manner, the evapotranspiration of all sugarcane cultivars, such that the days with low cloud provided the highest evapotranspiration values and the greater reduction among salinity levels. Analysis of the evapotranspiration accumulated during the daytime period showed that there were no significant differences among sugarcane cultivars and that, under the highest atmospheric water demand, the evapotranspiration decreased 48.5 g plant -1 EC -1 . This value represents the average reduction of 5.1% EC -1 when compared to the treatment without NaCl, considering data of all cultivars

A Methodology for Assessing Eco-efficiency in Logistics Networks

Recent literature on sustainable logistics networks points to two important questions: (i) How to spot the preferred solution(s) balancing environmental and business concerns? (ii) How to improve the understanding of the trade-offs between these two dimensions? We posit that a complete exploration of the efficient frontier and trade-offs between profitability and environmental impacts are particularly suitable to answer these two questions. In order to deal with the exponential number of basic efficient points in the frontier, we propose a formulation that performs in exponential time for the number of objective functions only. We illustrate our findings by designing a complex recycling logistics network in Germany

Search for bioindicators of pollution in the Guanabara Bay: integrations of ecologic patterns

Guanabara Bay, since its discovery, has largely changed with the human occupation causing large amounts of deposited sediment and waste, as well as domestic and industry sewage. Surface sediment was analysed for foraminifera and ostracoda distribution, diversity and dominance studies. These results were compared with TOC analyses aiming the determination of pollution bioindicators. In general, foraminifera dominant species were Ammonia tepida, Buliminella elegantissima and Quinqueloculina seminulum. The foraminifera assemblages presented distinct abundance and diversity values in different regions of the bay. The diversity was higher in the entrance (south) and in the central region than in the north region of the bay. The dominant species, that are characteristic of stressed environments, presented higher values of abundance in the north region. The TOC values increased from south to north regions, and were inversely proportional to foraminifera diversity. The very high TOC values in very polluted areas suggest sediment deposition in anoxic-dysoxic environment. The ostracoda Gen. Cyprideis was dominant and its occurrence increased from south to north region. Occurrence of Callistocythere sigmocostata, Xestoleberis sp., Aurila sp., and Paracypris sp. were restricted to the entrance and central area, indicating a preference for less restricted conditions, like marine conditions. Foraminifera and ostracoda characteristic responses to the environment conditions related high TOC values showed their importance as bioindicators of stressed environments caused by anthropogenic pollution, in the Guanabara Bay.A Baía de Guanabara, desde o seu descobrimento, tem sido bastante modificada pela ocupação humana, causando o acúmulo de grande quantidade de sedimento depositado e lixo, bem como lançamento de esgotos domésticos e industriais. Sedimentos superficiais foram analisados visando o estudo da distribuição, diversidade e dominância de foraminíferos e ostracodes. Estes estudos foram comparados com análises de COT objetivando a determinação de bioindicadores de poluição. Em geral as espécies dominantes de foraminíferos foram Ammonia tepida,Buliminella elegantissima and Quinqueloculina seminulum. As associações de foraminíferos apresentam distintos valores de abundância e diversidade em diferentes regiões da Baía. A diversidade foi mais elevada na entrada (sul) e na região central do que na região norte da Baía. As espécies dominantes, que são características de ambientes sob estresse, apresentaram valores altos de abundância na região norte. Os valores de COT aumentaram da região sul para o norte e foram inversamente proporcionais à diversidade de foraminíferos. O elevados valores de COT em áreas muito poluídas sugerem deposição sedimentar em ambiente anóxico-desóxico. O Gen. Cyprideis, de ostracode, foi dominante e sua ocorrência aumentou da região sul para o norte. A ocorrência de Callistocythere sigmocostata, Xestoleberis sp., Aurila sp. e Paracypris sp. foi estrita à entrada e região central, indicando uma preferência por condições menos restritas, como condições marinhas. As respostas características dos foraminíferos e ostracodes às condições ambientais relacionadas aos elevados valores de COT mostraram sua importância como bioindicadores de ambientes sob estresse causado por poluição antropogênica na Baía de Guanabara

The use of digital photography for the definition of coastal biotopes in Azores.

Copyright © Springer Science+Business Media B.V. 2007.Sampling benthic communities usually requires intensive field and lab work which is generally performed by skilled staff. In algal dominated communities, like those on the shores of the Azores, biotope characterization studies focused on the more conspicuous algae categories, thus reducing the skills required for species identification. The present study compares in situ quadrat quantifications done by a skilled reader, with computer based quadrat quantifications using digital photographic records of the same areas read in situ, accomplished by skilled and non-skilled readers. The study was conducted inter- and subtidally at various shore heights/depths. Quantification of algal coverage, both in situ and computer based, used the point to point method with quadrats of 0.25 m × 0.25 m for the intertidal, and 0.50 m × 0.50 m for the subtidal surveys, both subdivided into 36 intersection points. Significant differences were found between in situ readings and computer based readings of photographic records conducted both by experienced and inexperienced readers. Biotopes identified using in situ data and image based data differ both for the subtidal and intertidal

Modeling the quantum evolution of the universe through classical matter

It is well known that the canonical quantization of the Friedmann-Lema\^itre-Robertson-Walker (FLRW) filled with a perfect fluid leads to nonsingular universes which, for later times, behave as their classical counterpart. This means that the expectation value of the scale factor $(t)$ never vanishes and, as $t\to\infty$, we recover the classical expression for the scale factor. In this paper, we show that such universes can be reproduced by classical cosmology given that the universe is filled with an exotic matter. In the case of a perfect fluid, we find an implicit equation of state (EoS). We then show that this single fluid with an implict EoS is equivalent to two non-interacting fluids, one of them representing stiff matter with negative energy density. In the case of two non-interacting scalar fields, one of them of the phantom type, we find their potential energy. In both cases we find that quantum mechanics changes completely the configuration of matter for small values of time, by adding a fluid or a scalar field with negative energy density. As time passes, the density of negative energy decreases and we recover the ordinary content of the classical universe. The more the initial wave function of the universe is concentrated around the classical big bang singularity, the more it is necessary to add negative energy, since this type of energy will be responsible for the removal of the classical singularity.Comment: updated version as accepted by Gen. Relativ. Gravi

Subgingival Microbiome Colonization and Cytokine Production during Early Dental Implant Healing

Little is known about longitudinal development of the peri-implant subgingival microbiome and cytokine production as a new sulcus forms after dental implant placement. Therefore, the purpose of this observational study was to evaluate simultaneous longitudinal changes in the oral microbiome and cytokine production in the developing peri-implant sulcus compared to control natural teeth. Four and 12 weeks after implant placement and abutment connection, a dental implant and a natural tooth were sampled in 25 patients for subgingival plaque and gingival crevicular fluid (GCF [around teeth] and peri-implant crevicular fluid [PICF] around implants). DNA from plaque samples was extracted and sequenced using Illumina-based 16S rRNA sequencing. GCF and PICF samples were analyzed using a customized Milliplex human cytokine and chemokine magnetic bead panel. Beta diversity analysis revealed that natural teeth and implants had similar subgingival microbiomes, while teeth had greater alpha diversity than implants. At the genus level, however, few differences were noted between teeth and dental implants over 12 weeks. Specifically, Actinomyces and Selenomonas were significantly elevated around teeth versus dental implants at both 4 weeks and 12 weeks, while Corynebacterium and Campylobacter were significantly elevated only at 4 weeks around teeth. The only difference between PICF and GCF biomarkers was significantly elevated granulocyte-macrophage colony-stimulating factor levels around teeth versus dental implants at the 4-week visit. The subgingival microbiome and cytokine production were similar between teeth and implants during early healing, suggesting that these profiles are driven by the patient following dental implant placement and are not determined by anatomical niche