Avaliação sócio-ambiental da integração tecnológica Embrapa Pecuária Sudeste para produção leiteira na agricultura familiar.

Duas características da pecuária leiteira podem ser destacadas na atualidade: a) o baixo valor do leite, que dificulta a adoção de tecnologias que favoreçam a produtividade e b) a concentração da produção leiteira em estabelecimentos pequenos, nos quais predomina a atividade em nível de subsistência, com mão-de-obra familiar e renda mensal que não ultrapassa um salário mínimo. Um projeto de pesquisa e transferência de tecnologia da Embrapa Pecuária Sudeste (São Carlos, Estado de São Paulo) vem sendo desenvolvido desde 1998 para melhoria desse cenário. Uma vez implantado o projeto em uma série de estabelecimentos na região de Votuporanga, Estado de São Paulo, desenvolveu-se este estudo, para avaliar os impactos sócio-ambientais da adoção da tecnologia. Os resultados indicam que o desempenho dos estabelecimentos melhora em função do tempo desde a adoção e que a tecnologia contribui positivamente para o desenvolvimento sustentável, sendo recomendada para transferência

Time-Based Partitioning Model for Predicting Neurologically Favorable Outcome among Adults with Witnessed Bystander Out-of-Hospital CPA

Background：Optimal acceptable time intervals from collapse to bystander cardiopulmonary resuscitation (CPR) for neurologically favorable outcome among adults with witnessed out-of-hospital cardiopulmonary arrest (CPA) have been unclear. Our aim was to assess the optimal acceptable thresholds of the time intervals of CPR for neurologically favorable outcome and survival using a recursive partitioning model.Methods and Findings：From January 1, 2005 through December 31, 2009, we conducted a prospective population-based observational study across Japan involving consecutive out-of-hospital CPA patients (N = 69,648) who received a witnessed bystander CPR. Of 69,648 patients, 34,605 were assigned to the derivation data set and 35,043 to the validation data set. Time factors associated with better outcomes: the better outcomes were survival and neurologically favorable outcome at one month, defined as category one (good cerebral performance) or two (moderate cerebral disability) of the cerebral performance categories. Based on the recursive partitioning model from the derivation dataset (n = 34,605) to predict the neurologically favorable outcome at one month, 5 min threshold was the acceptable time interval from collapse to CPR initiation; 11 min from collapse to ambulance arrival; 18 min from collapse to return of spontaneous circulation (ROSC); and 19 min from collapse to hospital arrival. Among the validation dataset (n = 35,043), 209/2,292 (9.1%) in all patients with the acceptable time intervals and 1,388/2,706 (52.1%) in the subgroup with the acceptable time intervals and pre-hospital ROSC showed neurologically favorable outcome.Conclusions：Initiation of CPR should be within 5 min for obtaining neurologically favorable outcome among adults with witnessed out-of-hospital CPA. Patients with the acceptable time intervals of bystander CPR and pre-hospital ROSC within 18 min could have 50% chance of neurologically favorable outcome

Associated molecular and atomic clouds with X-ray shell of superbubble 30 Doradus C in the LMC

30 Doradus C is a superbubble which emits the brightest nonthermal X- and TeV gamma-rays in the Local Group. In order to explore detailed connection between the high energy radiation and the interstellar medium, we have carried out new CO and HI observations using the Atacama Large Millimeter$/$Submillimeter Array (ALMA), Atacama Submillimeter Telescope Experiment, and the Australia Telescope Compact Array with resolutions of up to 3 pc. The ALMA data of $^{12}$CO($J$ = 1-0) emission revealed 23 molecular clouds with the typical diameters of $\sim$6-12 pc and masses of $\sim$600-10000 $M_{\odot}$. The comparison with the X-rays of $XMM$-$Newton$ at $\sim$3 pc resolution shows that X-rays are enhanced toward these clouds. The CO data were combined with the HI to estimate the total interstellar protons. Comparison of the interstellar proton column density and the X-rays revealed that the X-rays are enhanced with the total proton. These are most likely due to the shock-cloud interaction modeled by the magnetohydrodynamical simulations (Inoue et al. 2012, ApJ, 744, 71). Further, we note a trend that the X-ray photon index varies with distance from the center of the high-mass star cluster, suggesting that the cosmic-ray electrons are accelerated by one or multiple supernovae in the cluster. Based on these results we discuss the role of the interstellar medium in cosmic-ray particle acceleration.Comment: 20 pages, 14 figures, 3 tables, accepted for publication in The Astrophysical Journa

Structural insights into cardiolipin transfer from the Inner membrane to the outer membrane by PbgA in Gram-negative bacteria

The outer membrane (OM) of Gram-negative bacteria is a unique asymmetric lipid bilayer in which the outer leaflet is composed of lipopolysaccharide (LPS) and the inner leaflet is formed by glycerophospholipid (GPL). The OM plays a fundamental role in protecting Gram-negative bacteria from harsh environments and toxic compounds. The transport and assembly pathways for phospholipids of bacterial OM are unknown. Cardiolipin (CL) plays an important role in OM biogenesis and pathogenesis, and the inner membrane (IM) protein PbgA, containing five transmembrane domains and a globular domain in periplasm has been recently identified as a CL transporter from the IM to the OM with an unknown mechanism. Here we present the first two crystal structures of soluble periplasmic globular domain of PbgA from S. typhimurium and E. coli, which revealed that the globular domains of PbgA resemble the structures of the arylsulfatase protein family and contains a novel core hydrophobic pocket that may be responsible for binding and transporting CLs. Our structural and functional studies shed an important light on the mechanism of CL transport in Gram-negative bacteria from the IM to the OM, which offers great potential for the development of novel antibiotics against multi-drug resistant bacterial infections

Measurement of the strong interaction induced shift and width of the 1s state of kaonic deuterium at J-PARC

The antikaon-nucleon interaction close to threshold provides crucial information on the interplay between spontaneous and explicit chiral symmetry breaking in low-energy QCD. In this context the importance of kaonic deuterium X-ray spectroscopy has been well recognized, but no experimental results have yet been obtained due to the difficulty of the measurement. We propose to measure the shift and width of the kaonic deuterium 1s state with an accuracy of 60 eV and 140 eV respectively at J-PARC. These results together with the kaonic hydrogen data (KpX at KEK, DEAR and SIDDHARTA at DAFNE) will then permit the determination of values of both the isospin I=0 and I=1 antikaon-nucleon scattering lengths and will provide the most stringent constraints on the antikaon-nucleon interaction, promising a breakthrough. Refined Monte Carlo studies were performed, including the investigation of background suppression factors for the described setup. These studies have demonstrated the feasibility of determining the shift and width of the kaonic deuterium atom 1s state with the desired accuracy of 60 eV and 140 eV.Comment: 12 pages, 9 figure

Development of Social Vocalizations in Mice

Adult mice are highly vocal animals, with both males and females vocalizing in same sex and cross sex social encounters. Mouse pups are also highly vocal, producing isolation vocalizations when they are cold or removed from the nest. This study examined patterns in the development of pup isolation vocalizations, and compared these to adult vocalizations. In three litters of CBA/CaJ mice, we recorded isolation vocalizations at ages postnatal day 5 (p5), p7, p9, p11, and p13. Adult vocalizations were obtained in a variety of social situations. Altogether, 28,384 discrete vocal signals were recorded using high-frequency-sensitive equipment and analyzed for syllable type, spectral and temporal features, and the temporal sequencing within bouts. We found that pups produced all but one of the 11 syllable types recorded from adults. The proportions of syllable types changed developmentally, but even the youngest pups produced complex syllables with frequency-time variations. When all syllable types were pooled together for analysis, changes in the peak frequency or the duration of syllables were small, although significant, from p5 through p13. However, individual syllable types showed different, large patterns of change over development, requiring analysis of each syllable type separately. Most adult syllables were substantially lower in frequency and shorter in duration. As pups aged, the complexity of vocal bouts increased, with a greater tendency to switch between syllable types. Vocal bouts from older animals, p13 and adult, had significantly more sequential structure than those from younger mice. Overall, these results demonstrate substantial changes in social vocalizations with age. Future studies are required to identify whether these changes result from developmental processes affecting the vocal tract or control of vocalization, or from vocal learning. To provide a tool for further research, we developed a MATLAB program that generates bouts of vocalizations that correspond to mice of different ages

Charmless Hadronic Two-Body B Meson Decays

We report the results of a study of two-body B meson decays to the complete set of K pi, pi pi, and K K final states. The study is performed on a data sample of 31.7 +/- 0.3 million B B-bar events recorded on the Upsilon(4S) resonance by the Belle experiment at KEKB. We observe significant signals in all K pi final states and in the pi+ pi- and pi+ pi0 final states. We set limits on the pi0 pi0 and K K final states. A search is performed for partial-rate asymmetries between conjugate states for flavor-specific final states.Comment: Submitted to PR

Mesurement of the B0 - anti-B0 Mixing Parameter Delta m_d using Semileptonic B0 Decays

We present a measurement of the B^0-B^0bar mixing parameter Delta m_d using neutral B meson pairs in a 29.1 fb^{-1} data sample collected at the Upsilon(4S) resonance with the Belle detector at the KEKB asymmetric-energy e^+e^- collider. We exclusively reconstruct one neutral B meson in the semileptonic B^0 \to D^{*-}\ell^+\nu decay mode and identify the flavor of the accompanying B meson from its decay products. From the distribution of the time intervals between the two flavor-tagged B meson decay points, we obtain Delta m_d = (0.494 +- 0.012 +- 0.015) ps^{-1}, where the first error is statistical and the second error is systematic.Comment: 10 pages, 3 figures, Published in Phys.Rev.Lett. 89, 251803 (2002

Observation of B+- -> omega K+- Decay

We report the first observation of the charmless two-body mode $B^{\pm} \to \omega K^{\pm}$ decay, and a new measurement of the branching fraction for the $B^{\pm} \to \omega \pi^{\pm}$ decay. The measured branching fractions are ${\cal B} (B^{\pm} \to \omega K^{\pm}) = (9.2{}^{+2.6}_{-2.3}\pm 1.0) \times 10^{-6}$ and ${\cal B} (B^{\pm} \to \omega \pi^{\pm}) = (4.2{}^{+2.0}_{-1.8}\pm 0.5) \times 10^{-6}$. %and we set 90% confidence level upper limits of %${\cal B} (B^- \to \omega \pi^-) < 8.1\times 10^{-6}$. We also measure the partial rate asymmetry of $B^{\pm}\to\omega K^{\pm}$ decays and obtain ${\cal A}_{CP} = -0.21 \pm 0.28 \pm 0.03$. The results are based on a data sample of 29.4 fb$^{-1}$ collected on the $\Upsilon(4S)$ resonance by the Belle detector at the KEKB $e^{+} e^{-}$ collider.Comment: 5 pages, 4 figures, resubmitted to Phys. Rev. Let

Observation of Ds+ K- and evidence for Ds+ pi- final states in neutral B decays

We report the first observation of a B meson decay that is not accessible by a direct spectator process. The channel B0bar -> Ds+ K- is found in a sample of 85 timse 10^6 B Bbar events, collected with the Belle detector at KEKB, with a branching fraction Br(B0bar -> Ds+ K-)=(4.6^{+1.2}_{-1.1} +- 1.3) times 10^{-5}. We also obtain evidence for the B0 -> Ds+ pi- decay with branching fraction Br(B0 -> Ds+ pi-)=(2.4^{+1.0}_{-0.8} +- 0.7) times 10^{-5}. This value may be used to extract a model-dependent value of |Vub|.Comment: Accepted to publication in Phys. Rev. Let