Hedgehog signalling acts upstream of Laminin alpha1 transcription in the zebrafish paraxial mesoderm

Laminin-111 (α1β1γ1) is a member of the Laminin family of extra-cellular matrix proteins that comprises 16 members, components of basement membranes. Laminin-111, one of the first Laminin proteins synthesised during embryogenesis, is required for basement membrane deposition and has essential roles in tissue morphogenesis and patterning. Yet, the mechanisms controlling Laminin-111 expression are poorly understood. We generated a zebrafish transgenic reporter line that reproduces faithfully the expression pattern of lama1, the gene encoding Laminin α1, and we used this reporter line to investigate lama1 transcriptional regulation. Our findings established that lama1 expression is controlled by intronic enhancers, including an enhancer directing expression in the paraxial mesoderm, anterior spinal cord and hindbrain, located in intron 1. We show that Hedgehog signalling is necessary and sufficient for lama1 transcription in the paraxial mesoderm and identify putative Gli/Zic binding sites that may mediate this control. These findings uncover a conserved role for Hedgehog signalling in the control of basement membrane assembly via its transcriptional regulation of lama1, and provide a mechanism to coordinate muscle cell fate specification in the zebrafish embryo

Divergence in Dialogue

Copyright: 2014 Healey et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.This work was supported by the Economic and Social Research Council (ESRC; http://www.esrc.ac.uk/) through the DynDial project (Dynamics of Conversational Dialogue, RES-062-23-0962) and the Engineering and Physical Sciences Research Council (EPSRC; http://www.epsrc.ac.uk/) through the RISER project (Robust Incremental Semantic Resources for Dialogue, EP/J010383/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Spitzer Observations of Low Luminosity Isolated and Low Surface Brightness Galaxies

We examine the infrared properties of five low surface brightness galaxies (LSBGs) and compare them with related but higher surface brightness galaxies, using Spitzer Space Telescope images and spectra. All the LSBGs are detected in the 3.6 and 4.5um bands, representing the stellar population. All but one are detected at 5.8 and 8.0um, revealing emission from hot dust and aromatic molecules, though many are faint or point-like at these wavelengths. Detections of LSBGs at the far-infrared wavelengths, 24, 70, and 160um, are varied in morphology and brightness, with only two detections at 160um, resulting in highly varied spectral energy distributions. Consistent with previous expectations for these galaxies, we find that detectable dust components exist for only some LSBGs, with the strength of dust emission dependent on the existence of bright star forming regions. However, the far-infrared emission may be relatively weak compared with normal star-forming galaxies.Comment: 20 pages, 8 figures, accepted to Ap

Automatic Methods of Gas Analysis Depending upon Thermal Conductivity

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Deformation mechanisms of idealised cermets under multi-axial loading

The response of idealised cermets comprising approximately 60% by volume steel spheres in a Sn/Pb solder matrix is investigated under a range of axisymmetric compressive stress states. Digital volume correlation (DVC) analysis of X-ray micro-computed tomography scans (μ-CT), and the measured macroscopic stress-strain curves of the specimens revealed two deformation mechanisms. At low triaxialities the deformation is granular in nature, with dilation occurring within shear bands. Under higher imposed hydrostatic pressures, the deformation mechanism transitions to a more homogeneous incompressible mode. However, DVC analyses revealed that under all triaxialities there are regions with local dilatory and compaction responses, with the magnitude of dilation and the number of zones wherein dilation occurs decreasing with increasing triaxiality. Two numerical models are presented in order to clarify these mechanisms: (i) a periodic unit cell model comprising nearly rigid spherical particles in a porous metal matrix and (ii) a discrete element model comprising a large random aggregate of spheres connected by non-linear normal and tangential “springs”. The periodic unit cell model captured the measured stress-strain response with reasonable accuracy but under-predicted the observed dilation at the lower triaxialities, because the kinematic constraints imposed by the skeleton of rigid particles were not accurately accounted for in this model. By contrast, the discrete element model captured the kinematics and predicted both the overall levels of dilation and the simultaneous presence of both local compaction and dilatory regions with the specimens. However, the levels of dilation in this model are dependent on the assumed contact law between the spheres. Moreover, since the matrix is not explicitly included in the analysis, this model cannot be used to predict the stress-strain responses. These analyses have revealed that the complete constitutive response of cermets depends both on the kinematic constraints imposed by the particle aggregate skeleton, and the constraints imposed by the metal matrix filling the interstitial spaces in that skeleton.The authors are grateful to the Office of Naval Research (ONR) for their financial support through grant number N00014121063

Nonlinear imaging of damage in composite structures using sparse ultrasonic sensor arrays

In different engineering fields, there is a strong demand for diagnostic methods able to provide detailed information on material defects. Low velocity impact damage can considerably degrade the integrity of structural components and, if not detected, can result in catastrophic failures. This paper presents a nonlinear structural health monitoring imaging method, based on nonlinear elastic wave spectroscopy, for the detection and localisation of nonlinear signatures on a damaged composite structure. The proposed technique relies on the bispectral analysis of ultrasonic waveforms originated by a harmonic excitation and it allows for the evaluation of second order material nonlinearities due to the presence of cracks and delaminations. This nonlinear imaging technique was combined with a radial basis function approach in order to achieve an effective visualisation of the damage over the panel using only a limited number of acquisition points. The robustness of bispectral analysis was experimentally demonstrated on a damaged carbon fibre reinforced plastic (CFRP) composite panel, and the nonlinear source’s location was obtained with a high level of accuracy. Unlike other ultrasonic imaging methods for damage detection, this methodology does not require any baseline with the undamaged structure for the evaluation of the defect, nor a priori knowledge of the mechanical properties of the specimen

Nonlinear imaging of damage in composite structures using sparse ultrasonic sensor arrays

In different engineering fields, there is a strong demand for diagnostic methods able to provide detailed information on material defects. Low velocity impact damage can considerably degrade the integrity of structural components and, if not detected, can result in catastrophic failures. This paper presents a nonlinear structural health monitoring imaging method, based on nonlinear elastic wave spectroscopy, for the detection and localisation of nonlinear signatures on a damaged composite structure. The proposed technique relies on the bispectral analysis of ultrasonic waveforms originated by a harmonic excitation and it allows for the evaluation of second order material nonlinearities due to the presence of cracks and delaminations. This nonlinear imaging technique was combined with a radial basis function approach in order to achieve an effective visualisation of the damage over the panel using only a limited number of acquisition points. The robustness of bispectral analysis was experimentally demonstrated on a damaged carbon fibre reinforced plastic (CFRP) composite panel, and the nonlinear source’s location was obtained with a high level of accuracy. Unlike other ultrasonic imaging methods for damage detection, this methodology does not require any baseline with the undamaged structure for the evaluation of the defect, nor a priori knowledge of the mechanical properties of the specimen

Radial HI Profiles at the Periphery of Galactic Disks: The Role of Ionizing Background Radiation

Observations of neutral hydrogen in spiral galaxies reveal a sharp cutoff in the radial density profile at some distance from the center. Using 22 galaxies with known HI distributions as an example, we discuss the question of whether this effect can be associated exclusively with external ionizing radiation, as is commonly assumed. We show that before the surface density reaches $\sigma_{\textrm{HI}}\le 0.5 {\cal M}_\odot/{\textrm {pc}}^2$(the same for galaxies of different types), it is hard to expect the gas to be fully ionized by background radiation. For two of 13 galaxies with a sharp drop in the HI profile, the "steepening" can actually be caused by ionization. At the same time, for the remaining galaxies, the observed cutoff in the radial HI profile is closer to the center than if it was a consequence of ionization by background radiation and, therefore, it should be caused by other factors.Comment: 15 pages, 6 figure

Is sticky blood bad for the Brain? Hemostatic and inflammatory systems and dementia in the Caerphilly prospective study

Objective— Hemostasis and inflammation have been implicated in dementia. This study investigates the role of specific hemostatic and inflammatory pathways with incident vascular and nonvascular dementia.Methods and Results— This was a prospective study of a population sample of men aged 65 to 84 years, with baseline assessment of hemostatic and inflammatory factors and cognition measured 17 years later. The sample included 865 men (59 had dementia and 112 had cognitive impairment, not dementia), free of vascular disease at baseline and for whom hemostatic and inflammatory marker data were available and cognitive status was known. A total of 15 hemostatic and 6 inflammatory markers were assessed. Factor analysis was used to identify hemostatic subsystems. The National Institute of Neurological Disorders and Stroke–Association Internationale pour la Recherche et l’Enseignement en Neurologie criteria were used to identify vascular dementia. By using standardized (z) scores for hemostatic and inflammatory markers, and after adjustment for age and risk factors, vascular dementia was associated with fibrinogen (hazard ratio [HR], 1.68; 95% confidence interval [CI], 1.02–2.76), factor VIII (HR, 1.79; 95% CI, 1.09–3.00), and plasminogen activator inhibitor 1 (HR, 3.13; 95% CI, 1.73–5.70). For vascular dementia, the HR risk from high levels of all three hemostatic variables (fibrinogen, factor VIII, and plasminogen activator inhibitor 1) was 2.97 (P<0.001). Inflammatory factors were not associated with vascular dementia.Conclusion— The associations of these hemostatic markers with vascular dementia may implicate clot formation as the primary mechanism and are consistent with a microinfarct model of vascular dementia

Vertical abundance stratification in the blue horizontal branch star HD135485

It is commonly believed that the observed overabundances of many chemical species relative to the expected cluster metallicity in blue horizontal branch (BHB) stars appear as a result of atomic diffusion in the photosphere. The slow rotation of BHB stars (with T_eff > 11,500K), typically v sin{i} < 10 km/s, is consistent with this idea. In this work we search for observational evidence of vertical chemical stratification in the atmosphere of HD135485. If this evidence exists, it will demonstrate the importance of atomic diffusion processes in the atmospheres of BHB stars. We undertake an extensive abundance stratification analysis of the atmosphere of HD135485, based on recently acquired high resolution and S/N CFHT ESPaDOnS spectra and a McDonald-CE spectrum. Our numerical simulations show that nitrogen and sulfur reveal signatures of vertical abundance stratification in the stellar atmosphere. It appears that the abundances of these elements increase toward the upper atmosphere. This fact cannot be explained by the influence of microturbulent velocity, because oxygen, carbon, neon, argon, titanium and chromium do not show similar behavior and their abundances remain constant throughout the atmosphere. It seems that the iron abundance may increase marginally toward the lower atmosphere. This is the first demonstration of vertical abundance stratification of metals in a BHB star.Comment: 8 pages, 5 figures, accepted to A&