Screening of at-risk blood donors for Chagas disease in non-endemic countries: Lessons from a 2-year experience in Tuscany, Italy

Background: Chagas disease (CD) is caused by the protozoan parasite Trypanosoma cruzi and is transmitted by blood-sucking triatomine insects in endemic areas of Latin America. Transmission can also occur via blood transfusion and is a major cause of CD in non-endemic areas. Objectives: The aim of the study was to assess the prevalence of anti-T. cruzi antibodies in blood donors at risk of infection in Tuscany, Italy, following the introduction of blood safety Italian legislation. Material and methods: Donors (N = 1985) were tested in 2016 to 2018 for anti-T. cruzi IgG using an immunochromatographic test (ICT). Chemiluminescent immunoassay (CLIA) was performed on ICT-positive donors to exclude CD, whereas enzyme-linked immunosorbent assay and Western blot were performed in case of discordant results. All assays were performed on CD patients (N = 10) for validation. Results: Ten blood donors had a positive ICT result, with a resulting T. cruzi seroprevalence of 0.5% but demonstrates negative results to CLIA, as well as to the other serological assays. The comparison of serological assays suggested a lower relative sensitivity of ICT. Conclusion: The results of this study confirm the significance of serological testing in the screening strategy for TT CD. However, they provide evidence for discontinuing the use of ICT as a screening test and suggest that a sensitive, specific and multi-sample format assay should be used at the national level for uniformity of results

Editorial: Exploring system justification phenomenon among disadvantaged individuals

The question of why (or even when) the disadvantaged might be more or less supportive of existing social arrangements is a matter of debate amongst social and political psychologists (e.g., Passini, 2019; Jost, 2020, see also Rubin et al., 2022). Accordingly, for this Research Topic, we chose a title that was deliberately broad in scope, accommodating several aspects that included: (a) the drivers of system justification; (b) the socio-structural conditions that enhance or dampen system justification, (c) the ideological correlates of system support, and (d) the impact of system justification on wellbeing. Taken together, the contributions comprised in this Research Topic provide a comprehensive analysis of these four issues

A deep attention based approach for predictive maintenance applications in IoT scenarios

Purpose: The recent innovations of Industry 4.0 have made it possible to easily collect data related to a production environment. In this context, information about industrial equipment – gathered by proper sensors – can be profitably used for supporting predictive maintenance (PdM) through the application of data-driven analytics based on artificial intelligence (AI) techniques. Although deep learning (DL) approaches have proven to be a quite effective solutions to the problem, one of the open research challenges remains – the design of PdM methods that are computationally efficient, and most importantly, applicable in real-world internet of things (IoT) scenarios, where they are required to be executable directly on the limited devices’ hardware. Design/methodology/approach: In this paper, the authors propose a DL approach for PdM task, which is based on a particular and very efficient architecture. The major novelty behind the proposed framework is to leverage a multi-head attention (MHA) mechanism to obtain both high results in terms of remaining useful life (RUL) estimation and low memory model storage requirements, providing the basis for a possible implementation directly on the equipment hardware. Findings: The achieved experimental results on the NASA dataset show how the authors’ approach outperforms in terms of effectiveness and efficiency the majority of the most diffused state-of-the-art techniques. Research limitations/implications: A comparison of the spatial and temporal complexity with a typical long-short term memory (LSTM) model and the state-of-the-art approaches was also done on the NASA dataset. Despite the authors’ approach achieving similar effectiveness results with respect to other approaches, it has a significantly smaller number of parameters, a smaller storage volume and lower training time. Practical implications: The proposed approach aims to find a compromise between effectiveness and efficiency, which is crucial in the industrial domain in which it is important to maximize the link between performance attained and resources allocated. The overall accuracy performances are also on par with the finest methods described in the literature. Originality/value: The proposed approach allows satisfying the requirements of modern embedded AI applications (reliability, low power consumption, etc.), finding a compromise between efficiency and effectiveness

The influence of chromium nitrides precipitation on the fatigue behavior of duplex stainless steels

This paper studies the fatigue behavior at room temperature of UNS S32205 and UNS S32750 duplex stainless steels (DSSs) under two thermal treatments. In both types of DSSs, thermal treatments at high temperature followed by water quenching (TTW) produces the precipitation of Cr2N within the ferrite phase. The amount of Cr2N increases mainly with the ferritic grain size independently of the nitrogen content. This nitride precipitation hardens the ferritic phase and produces a detrimental effect on the fatigue life of both steels.Fil: Hereñu, Silvina Andrea Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); ArgentinaFil: Armas, A. F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); ArgentinaFil: Alvarez, Iris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); ArgentinaFil: Moscato, M. G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); Argentin

Exploratory study of the EU-DEMO Water-Cooled Lithium Lead breeding blanket behaviour in case of loss of cooling capability

Within the framework of the European Roadmap to the realization of fusion energy, a strong international cooperation is ongoing to develop a Breeding Blanket (BB) system for the EU-DEMO reactor. Although it is still to be decided whether the DEMO in-vessel components should perform any safety function, the pursuing of robust blanket concepts able to handle upset and accidental loading conditions has been always seen as good practice in fusion reactor engineering to enhance the inherent plant safety performances. Amongst the several classes of events that might challenge the BB structural integrity, the large Loss of Coolant Accident is one of the most relevant because it usually leads to a fast loss of cooling capability of the structures. Due to the characteristic of the tokamak assembly, the behaviour of each blanket segment during a sudden loss of cooling capability does not depend only upon distinguishing features of the component itself. In fact, the overall transient can be governed by conditions established in surrounding elements, like adjacent blanket segments and vacuum vessel, as well as by the plasma shutdown strategies adopted to protect the reactor. The scope of the activity herein presented is to make a preliminary assessment of the intrinsic capability of EU-DEMO tokamak architecture to cope with the loss of cooling in the Water-Cooled Lithium Lead (WCLL) BB concept. Evaluation of BB thermal field in short and medium term under simplified, yet conservative, assumptions was carried out for four transient scenarios with the aim of investigating the response of the structure in case of: a) fast or soft plasma shutdown, and b) different blanket cooling schemes. Moreover, the WCLL BB thermo-mechanical response in the most critical time steps has been assessed. The obtained results shall help for future decisions on safety systems/action to be implemented to cope with accidents

Bronchoalveolar lavage, sputum and exhaled clinically relevant inflammatory markers: values in healthy adults.

11noBronchoalveolar lavage (BAL), induced sputum and exhaled breath markers (exhaled nitric oxide and exhaled breath condensate) can each provide biological insights into the pathogenesis of respiratory disorders. Some of their biomarkers are also employed in the clinical management of patients with various respiratory diseases. In the clinical context, however, defining normal values and cut-off points is crucial. The aim of the present review is to investigate to what extent the issue of defining normal values in healthy adults has been pursued for the biomarkers with clinical value. The current authors reviewed data from literature that specifically addressed the issue of normal values from healthy adults for the four methodologies. Most studies have been performed for BAL (n = 9), sputum (n = 3) and nitric oxide (n = 3). There are no published studies for breath condensate, none of whose markers yet has clinical value. In healthy adult nonsmokers the cut-off points (mean+2sd) for biomarkers with clinical value were as follows. BAL: 16.7% lymphocytes, 2.3% neutrophils and 1.9% eosinophils; sputum: 7.7 x 10(6).mL(-1) total cell count and 2.2% eosinophils; nitric oxide: 20.2 ppb. The methodologies differ concerning the quantity and characteristics of available reference data. Studies focusing on obtaining reference values from healthy individuals are still required, more evidently for the new, noninvasive methodologies.nonemixedBALBI B; PIGNATTI P; CORRADI M; BAIARDI P; BIANCHI L; BRUNETTI G; RADAELI A; MOSCATO G; MUTTI A; SPANEVELLO A; MALERBA MBalbi, B; Pignatti, P; Corradi, M; Baiardi, P; Bianchi, L; Brunetti, G; Radaeli, A; Moscato, G; Mutti, A; Spanevello, Antonio; Malerba, M

COVID 19: a clue from innate immunity

The recent COVID-19 pandemic has had a significant impact on our lives and has rapidly expanded to reach more than 4 million cases worldwide by May 2020. These cases are characterized by extreme variability, from a mild or asymptomatic form lasting for a few days up to severe forms of interstitial pneumonia that may require ventilatory therapy and can lead to patient death. Several hypotheses have been drawn up to understand the role of the interaction between the infectious agent and the immune system in the development of the disease and the most severe forms; the role of the cytokine storm seems important. Innate immunity, as one of the first elements of guest interaction with different infectious agents, could play an important role in the development of the cytokine storm and be responsible for boosting more severe forms. Therefore, it seems important to study also this important arm of the immune system to adequately understand the pathogenesis of the disease. Research on this topic is also needed to develop therapeutic strategies for treatment of this disease

Barium titanate nanoparticles and hypergravity stimulation improve differentiation of mesenchymal stem cells into osteoblasts.

BACKGROUND: Enhancement of the osteogenic potential of mesenchymal stem cells (MSCs) is highly desirable in the field of bone regeneration. This paper proposes a new approach for the improvement of osteogenesis combining hypergravity with osteoinductive nanoparticles (NPs). MATERIALS AND METHODS: In this study, we aimed to investigate the combined effects of hypergravity and barium titanate NPs (BTNPs) on the osteogenic differentiation of rat MSCs, and the hypergravity effects on NP internalization. To obtain the hypergravity condition, we used a large-diameter centrifuge in the presence of a BTNP-doped culture medium. We analyzed cell morphology and NP internalization with immunofluorescent staining and coherent anti-Stokes Raman scattering, respectively. Moreover, cell differentiation was evaluated both at the gene level with quantitative real-time reverse-transcription polymerase chain reaction and at the protein level with Western blotting. RESULTS: Following a 20 g treatment, we found alterations in cytoskeleton conformation, cellular shape and morphology, as well as a significant increment of expression of osteoblastic markers both at the gene and protein levels, jointly pointing to a substantial increment of NP uptake. Taken together, our findings suggest a synergistic effect of hypergravity and BTNPs in the enhancement of the osteogenic differentiation of MSCs. CONCLUSION: The obtained results could become useful in the design of new approaches in bone-tissue engineering, as well as for in vitro drug-delivery strategies where an increment of nanocarrier internalization could result in a higher drug uptake by cell and/or tissue constructs