164 research outputs found
Agrobacterium mediated transformation of annexin gene in tobacco (Nicotiana tabacum)
The present study involves the development of genetically engineered tobacco plants with annexin gene. The plasmid pUC 119 with the Annexin gene and pGPTV vector were isolated from the Escherichia coli. These plasmids were subjected to restriction digestion with EcoRI and Xbal where the Annexin gene is released from the pUC 119 as a linearised band was eluted from the gel. The recombinant PGPTV plasmid with the annexin gene in Agrobacterium tumefaciens MTCC 431 was mobilized and transferred to plant system through the mobilization helper plasmid pRK2013. The kanamycin resistance gene (NPT II) was used as a selective marker. The calli used for isolating the genomic DNA which was then amplified for confirmation of annexin gene. The nptII gene of 800 bp serves as a selectable marker system in plants and its amplification confirmed the presence of annexin gene in transgenic plants by PCR method
Ricci Solitons in f-Kenmotsu Manifolds and 3-dimensional Trans-Sasakian Manifolds
In the Present paper we study Ricci solitons in trans-sasakian manifolds. In particular we consider Ricci solitons in f -Kenmotsu manifolds and we prove the conditions for the Ricci solitons to be shrinking, steady and expanding.Key words: Ricci solitons; f -Kenmotsu; Trans-Sasakian; Shrinking; Steady; Expandin
Nucleus Segmentation and Classification using Residual SE-UNet and Feature Concatenation Approach in Cervical Cytopathology Cell images
Basic Logic Gates in Two Dimensional Photonic Crystals for All Optical Device Design
The research carried out in the field of optics and photonics with an idea to design and develop the all optical logic devices in the fascinating material known as photonic crystals (PhCs). The structural investigation reveals that the two dimensional (2-D) PhCs is more suitable for fabrication of optoelectronic components. In this article we have designed basic logic gates in 2-D PhCs as they are the building blocks for the construction of optical devices and in these, refractive index is periodically modulated with the wavelength. The understanding of light behaviour in complex PhCs helps in creating photonic band gap (PBG) that can prevent light of certain wavelength propagating in crystal lattice structure. For the selected geometry structure, three PBG bands will exists out of which two of them are transverse electric (TE) and one is transverse magnetic (TM) mode. The PBG bands in the TE mode ranges from 0.31(a/λ) to 0.46(a/λ) , 0.61(a/λ) to 0.63(a/λ) and TM mode ranges from 0.86(a/λ) to 0.93(a/λ). The free space wavelength of 1550 nm is set for the finite difference time domain (FDTD) simulation of the structure. The response time and computational overhead required for the proposed OR gate is 0.128ps and 4.4MB is obtained. Also we calculated the extinction ratio for AND gate and NOT gate as 6.19 dB and 10.21 dB respectively
Automated skin lesion segmentation using multi-scale feature extraction scheme and dual-attention mechanism
Segmenting skin lesions from dermoscopic images is essential for diagnosing
skin cancer. But the automatic segmentation of these lesions is complicated due
to the poor contrast between the background and the lesion, image artifacts,
and unclear lesion boundaries. In this work, we present a deep learning model
for the segmentation of skin lesions from dermoscopic images. To deal with the
challenges of skin lesion characteristics, we designed a multi-scale feature
extraction module for extracting the discriminative features. Further in this
work, two attention mechanisms are developed to refine the post-upsampled
features and the features extracted by the encoder. This model is evaluated
using the ISIC2018 and ISBI2017 datasets. The proposed model outperformed all
the existing works and the top-ranked models in two competitions
Power Consumption and Carbon Emission Equivalent for Virtualized Resources – An Analysis: Virtual Machine and Container Analysis for Greener Data Center
The International Energy Agency (IEA) revealed that the worldwide energy-related carbon dioxide (CO2) situation has hit a historic high of 33.1 Giga tonnes (Gt) of CO2. 85% of the rise in emissions was due to China, India, and the United States. The increase in emissions in India was 4.8%, or 105 Mega tonnes (Mt) of CO2, with the increase in emissions being evenly distributed across the transportation and industrial sectors, according to Beloglazov et al (2011). Environmental contamination brought on by carbon emissions is harmful to the environment. As a result, there is an urgent need for the IT sectors to develop effective and efficient technology to eliminate such carbon emissions. The primary focus is on lowering carbon emissions due to widespread awareness of the issue
Utility of Bacillary Index in Slit Skin Smears in Correlation with Clinical and Histopathological Alterations in Hansen’s Disease: An Attempt to Revive a Simple Useful Procedure
Leprosy, a relatively common chronic contagious disease having diverse modes of clinical presentation, can mimic a variety of unrelated diseases. For proper and adequate treatment, the diagnosis must be made accurately with subtyping which should be done with the help of bacillary index, histopathological features, and clinical correlation. This is extremely important in patient care as paucibacillary and multibacillary types have different modes of treatment. Our aim is to categorize leprosy into various types based on bacillary index, morphological findings both in slit skin smears, and biopsy along with clinical correlation.Keywords: Bacillary index, Histopathology, Leprosy, Multibacillary, Paucibacillary, Slit skin smear
Atypical presentation of Niemann-Pick disease
Niemann-Pick (NP) disease is a distinct disorder with specific clinical and morphological manifestations. We report a 2-year-oldgirl who presented with normal development, acute onset of pallor, lethargy, and massive splenomegaly, which made us to think ofhemolytic anemia as the initial diagnosis. Her bone marrow done, after initial hematological work up being normal, showed NP cells.As the child did not have any neurological abnormality, she was diagnosed as NP Type B (NPD-B) storage disease. We report it as atypical presentation of NPD-B
- …