Isolation, identification and bioactive potential of bacterial endophytes from Coleus

Coleus (Lamiaceae) is a large and widespread genus comprising of species with diverse ethnobotanical uses. In the present study, bacterial endophytes were isolated from Coleus forskohlii and Coleus aromaticus. Endophytes are the microorganisms which reside within the plants without showing any harmful effect on its host. Diverse types of endophytes live symbiotically within almost all plants and in turn help the plant in a number of ways such as imparting resistance against biotic and abiotic stresses, producing compounds involved in attraction of pollinators, inducing the plant defense mechanisms, etc. The bacterial endophytes isolated in this study, were characterized by microscopic examination (using gram staining) and molecularly identified by sequencing the 16S rRNA. Extracts were prepared from endophytic biomass using solvents of different polarities (methanol, ethyl acetate and butanol) and were screened for their bioactive potential (in vitro cytotoxicity anti-microbial, and anti-oxidant activity). Scale-up of endophytes showing promising results is under process, which will help in isolation of pure compounds

Circular Economy Pathways for Municipal Wastewater Management in India: A Practitioner’s Guide

The 2030 Water Resources Group is a unique publicprivate- civil society partnership that helps governments to accelerate reforms that will ensure sustainable water resource management for the long term development and economic growth of their country. It does so by helping to change the “political economy” for water reform in the country through convening a wide range of actors and providing water resource analysis in ways that are digestible for politicians and business leaders. The 2030 WRG was launched in 2008 at the World Economic Forum and has been hosted by the International Finance Corporation (IFC) since 2012

Human Antibodies that Slow Erythrocyte Invasion Potentiate Malaria-Neutralizing Antibodies.

The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) is the leading target for next-generation vaccines against the disease-causing blood-stage of malaria. However, little is known about how human antibodies confer functional immunity against this antigen. We isolated a panel of human monoclonal antibodies (mAbs) against PfRH5 from peripheral blood B cells from vaccinees in the first clinical trial of a PfRH5-based vaccine. We identified a subset of mAbs with neutralizing activity that bind to three distinct sites and another subset of mAbs that are non-functional, or even antagonistic to neutralizing antibodies. We also identify the epitope of a novel group of non-neutralizing antibodies that significantly reduce the speed of red blood cell invasion by the merozoite, thereby potentiating the effect of all neutralizing PfRH5 antibodies as well as synergizing with antibodies targeting other malaria invasion proteins. Our results provide a roadmap for structure-guided vaccine development to maximize antibody efficacy against blood-stage malaria. Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved

Upper limb rehabilitation using robotic exoskeleton systems: a systematic review

Exoskeleton assisted therapy has been reported as a significant reduction in impairment and gain in functional abilities of stroke patients. In this paper, we conduct a systematic review on the upper limb rehabilitation using robotic exoskeleton systems. This review is based on typical mechanical structures and control strategies for exoskeletons in clinical rehabilitation conditions. A variety of upper limb exoskeletons are classified and reviewed according to their rehabilitation joints. Special attentions are paid to the performance control strategies and mechanism designs in clinical trials and to promote the adaptability to different patients and conditions. Finally, we analyze and highlight the current research gaps and the future directions in this field. We intend to offer informative resources and reliable guidance for relevant researcher’s further studies, and exert a far-reaching influence on the development of advanced upper limb exoskeleton robotic systems

Hydromagnetic thermal instability of compressible Walters' (Model B') rotating fluid permeated with suspended particles in porous medium

In this paper, the thermal instability of compressible Walters’ (Model B′) rotating fluid permeated with suspended particles (fine dust) in porous medium in hydromagnetics is considered. By applying normal mode analysis method, the dispersion relation has been derived and solved analytically. It is observed that the rotation, magnetic field, suspended particles and viscoelasticity introduce oscillatory modes. For stationary convection, Walters’ (Model B′) elastico-viscous fluid behaves like an ordinary Newtonian fluid and it is observed that rotation has stabilizing effect, suspended particles are found to have destabilizing effect on the system, whereas the medium permeability has stabilizing or destabilizing effect on the system under certain conditions. The magnetic field has destabilizing effect in the absence of rotation, whereas in the presence of rotation, magnetic field has stabilizing or destabilizing effect under certain conditions

An intrinsically compliant robotic orthosis for treadmill training

A new intrinsically compliant robotic orthosis powered by pneumatic muscle actuators (PMA) was developed for treadmill training of neurologically impaired subjects. The robotic orthosis has hip and knee sagittal plane rotations actuated by antagonistic configuration of PMA. The orthosis has passive mechanisms to allow vertical and lateral translations of the trunk and a passive hip abduction/adduction joint. A foot lifter having a passive spring mechanism was used to ensure sufficient foot clearance during swing phase. A trajectory tracking controller was implemented to evaluate the performance of the robotic orthosis on a healthy subject. The results show that the robotic orthosis is able to perform the treadmill training task by providing sufficient torques to achieve physiological gait patterns and a realistic stepping experience. The orthosis is a new addition to the rapidly advancing field of robotic orthoses for treadmill training