GeoBoids: A Mobile AR Application for Exergaming

“© © 2012 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.”We have designed a mobile Augmented Reality (AR) game which incorporates video see-through and spatialized audio AR techniques and encourages player movement in the real world. In the game, called GeoBoids, the player is surrounded by flocks of virtual creatures that are visible and audible through mobile AR application. The goal is for the player to run to the location of a GeoBoid swarm in the real world, capture all the creatures there, then run to the next swarm and repeat, before time runs out, encouraging the player to exercise during game play. The most novel elements of the game are the use of audio input and output for interacting with the creatures. The interface design of the game includes AR visualization, spatialized audio, touch gestures and whistle interaction. Feedback from users in a preliminary user study was mostly positive on overall game play and the design of the UI, while the results also revealed improvements were needed for whistle interaction and the visual design of the GeoBoids

RecA-mediated strand invasion of DNA by oligonucleotides substituted with 2-aminoadenine and 2-thiothymine

Sequence-specific recognition of DNA is a critical step in gene targeting. Here we describe unique oligonucleotide (ON) hybrids that can stably pair to both strands of a linear DNA target in a RecA-dependent reaction with ATP or ATPγS. One strand of the hybrids is a 30-mer DNA ON that contains a 15-nt-long A/T-rich central core. The core sequence, which is substituted with 2-aminoadenine and 2-thiothymine, is weakly hybridized to complementary locked nucleic acid or 2′-OMe RNA ONs that are also substituted with the same base analogs. Robust targeting reactions took place in the presence of ATPγS and generated metastable double D-loop joints. Since the hybrids had pseudocomplementary character, the component ONs hybridized less strongly to each other than to complementary target DNA sequences composed of regular bases. This difference in pairing strength promoted the formation of joints capable of accommodating a single mismatch. If similar joints can form in vivo, virtually any A/T-rich site in genomic DNA could be selectively targeted. By designing the constructs so that the DNA ON is mismatched to its complementary sequence in DNA, joint formation might allow the ON to function as a template for targeted point mutation and gene correction

Valutazione della qualità e dello stato di conservazione degli ambienti litoranei: l'esempio del pS.I.C. "Penisola del Cavallino: biotopi litoranei" (Venezia, NE-Italia)

Evaluation of quality and conservation status in coastal landscapes: the example of the Site of Community Importance “Penisola del Cavallino: biotopi litoranei”(Venice, NE Italy). Quality of coastal landscape has been estimated at two different scale: at the community level, we assessed a naturalistic value, by evaluating characteristics coming both from species (i.e. presence of endemic species) and from community itself (i.e. rarity). We also took into account the percentage of exotic and ruderal species on the total number of species of each community, as an indirect measure of alteration degree. At the landscape level, we tried to value structural and functional integrity by evaluating spatial connectivity and contrast among patches. As the plant communities of foredunes show a typical spatial zonation, the two indices have been calculated along 27 transects perpendicular to the seashore. On the basis of a digital map of the area, we measured the gamma connectivity index along each transect; we assessed both “total connectivity”, considering all the communities intersected by the transects, and “partial connectivity”, considering only the plant communitie s of foredunes, so describing spatial distribution model of coastal vegetation. Since on the backdune there is not a typical zonation of communiti es, we counted only the links between natural communities, considering anthropogenic communities as a break of natural landscape and of connectivity. I n addition, we measured the contrast index; this index evaluates the change in environmental conditions going from one community (or patch) to the neighbouring one. The different patches are included and surrounded by a matrix, consisting of “nonhabitat” at different degree of alteratio n, that may function as an impenetrable barrier for individuals or, on the contrary, may favour movements. Thus, the surrounding matrix may significant ly influence the effective isolation of habitat patches, rendering them more or less isolated than simple distance would indicate. For the contr ast computation, we utilized the naturalistic value assessed to each community on the basis of the phytosociological data. Both indices point to the extreme fragmentation of coastal landscape and to high vulnerability that characterizes all natural communities being they surrounded by a matrix hig hly contrasting. Conservation strategies should, then, concern natural habitat, their quality and their structural and functional integrity, but they should also consider the quality of surrounding matrix

Phytocoenotic diversity of the N-Adriatic coastal sand dunes - The herbaceous communities of the fixed dunes and the vegetation of the interdunal wetlands

The hemicryptophytic, chamaephytic and therophytic communities which inhabit the fixed sand dunes of the N-Adriatic coast, along with those of the interdunal wetlands, have been studied from the phytosociological point of view. The floristic-sociological analysis focuses on the syntaxonomic discussion of the xerophilous communities; in this context, the following new syntaxa have been described: Syntrichio ruraliformis-Lomelosion argenteae, Tortulo-Scabiosetum typicum and Sileno conicae-Avellinietum michelii. In regards to the dune slack vegetation, the study points out the occurrence in the coastal area of two rare inland microtherm communities (Plantagini altissimae-Molinietum caeruleae and Erucastro-Schoenetum nigricantis). As already stated in previous papers, the originality of this sector of the Mediterranean basin is highlighted as a result of both bioclimatic and phytogeographic factors, which confer to this area a unique character in the European context

Identification of the protein kinases Pyk3 and Phg2 as regulators of the STATc-mediated response to hyperosmolarity

Cellular adaptation to changes in environmental osmolarity is crucial for cell survival. In Dictyostelium, STATc is a key regulator of the transcriptional response to hyperosmotic stress. Its phosphorylation and consequent activation is controlled by two signaling branches, one cGMP- and the other Ca(2+)-dependent, of which many signaling components have yet to be identified. The STATc stress signalling pathway feeds back on itself by upregulating the expression of STATc and STATc-regulated genes. Based on microarray studies we chose two tyrosine-kinase like proteins, Pyk3 and Phg2, as possible modulators of STATc phosphorylation and generated single and double knock-out mutants to them. Transcriptional regulation of STATc and STATc dependent genes was disturbed in pyk3(-), phg2(-), and pyk3(-)/phg2(-) cells. The absence of Pyk3 and/or Phg2 resulted in diminished or completely abolished increased transcription of STATc dependent genes in response to sorbitol, 8-Br-cGMP and the Ca(2+) liberator BHQ. Also, phospho-STATc levels were significantly reduced in pyk3(-) and phg2(-) cells and even further decreased in pyk3(-)/phg2(-) cells. The reduced phosphorylation was mirrored by a significant delay in nuclear translocation of GFP-STATc. The protein tyrosine phosphatase 3 (PTP3), which dephosphorylates and inhibits STATc, is inhibited by stress-induced phosphorylation on S448 and S747. Use of phosphoserine specific antibodies showed that Phg2 but not Pyk3 is involved in the phosphorylation of PTP3 on S747. In pull-down assays Phg2 and PTP3 interact directly, suggesting that Phg2 phosphorylates PTP3 on S747 in vivo. Phosphorylation of S448 was unchanged in phg2(-) cells. We show that Phg2 and an, as yet unknown, S448 protein kinase are responsible for PTP3 phosphorylation and hence its inhibition, and that Pyk3 is involved in the regulation of STATc by either directly or indirectly activating it. Our results add further complexities to the regulation of STATc, which presumably ensure its optimal activation in response to different environmental cues

The ALPS project release 1.3: open source software for strongly correlated systems

We present release 1.3 of the ALPS (Algorithms and Libraries for Physics Simulations) project, an international open source software project to develop libraries and application programs for the simulation of strongly correlated quantum lattice models such as quantum magnets, lattice bosons, and strongly correlated fermion systems. Development is centered on common XML and binary data formats, on libraries to simplify and speed up code development, and on full-featured simulation programs. The programs enable non-experts to start carrying out numerical simulations by providing basic implementations of the important algorithms for quantum lattice models: classical and quantum Monte Carlo (QMC) using non-local updates, extended ensemble simulations, exact and full diagonalization (ED), as well as the density matrix renormalization group (DMRG). Changes in the new release include a DMRG program for interacting models, support for translation symmetries in the diagonalization programs, the ability to define custom measurement operators, and support for inhomogeneous systems, such as lattice models with traps. The software is available from our web server at http://alps.comp-phys.org/