66 research outputs found

    Towards illiberal conditioning? New politics of media regulations in Poland (2015–2018)

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    In this article, we examine how media policy changes aid de-democratisation in Poland. Unfolding the logic underpinning the new politics of media regulations, this article argues that media policy paints a nuanced picture of democratic backsliding. Our Foucault-inspired discourse analysis of media policy archive focuses on the rise of illiberal trends at the cross-roads of the Polish hybrid media system, democracy and society. We find these trends display the features of centralisation of power, cultural politics, political partisanship and social polarisations. We explain these notions, using the concepts of “executive aggrandisement” and “politicisation” of public service media sector

    Fortunella margarita Transcriptional Reprogramming Triggered by Xanthomonas citri subsp. citri

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    <p>Abstract</p> <p>Background</p> <p>Citrus canker disease caused by the bacterial pathogen <it>Xanthomonas citri </it>subsp. <it>citri (</it>Xcc) <it>has </it>become endemic in areas where high temperature, rain, humidity, and windy conditions provide a favourable environment for the dissemination of the bacterium. Xcc is pathogenic on many commercial citrus varieties but appears to elicit an incompatible reaction on the citrus relative <it>Fortunella margarita </it>Swing (kumquat), in the form of a very distinct delayed necrotic response. We have developed subtractive libraries enriched in sequences expressed in kumquat leaves during both early and late stages of the disease. The isolated differentially expressed transcripts were subsequently sequenced. Our results demonstrate how the use of microarray expression profiling can help assign roles to previously uncharacterized genes and elucidate plant pathogenesis-response related mechanisms. This can be considered to be a case study in a citrus relative where high throughput technologies were utilized to understand defence mechanisms in <it>Fortunella </it>and citrus at the molecular level.</p> <p>Results</p> <p><b>cDNAs from sequenced kumquat libraries (ESTs) made from subtracted RNA populations, healthy vs. infected, were used to make this microarray</b>. Of 2054 selected genes on a customized array, 317 were differentially expressed (P < 0.05) in Xcc challenged kumquat plants compared to mock-inoculated ones. This study identified components of the incompatible interaction such as reactive oxygen species (ROS) and programmed cell death (PCD). Common defence mechanisms and a number of resistance genes were also identified. In addition, there were a considerable number of differentially regulated genes that had no homologues in the databases. This could be an indication of either a specialized set of genes employed by kumquat in response to canker disease or new defence mechanisms in citrus.</p> <p>Conclusion</p> <p>Functional categorization of kumquat Xcc-responsive genes revealed an enhanced defence-related metabolism as well as a number of resistant response-specific genes in the kumquat transcriptome in response to Xcc inoculation. Gene expression profile(s) were analyzed to assemble a comprehensive and inclusive image of the molecular interaction in the kumquat/Xcc system. This was done in order to elucidate molecular mechanisms associated with the development of the hypersensitive response phenotype in kumquat leaves. These data will be used to perform comparisons among citrus species to evaluate means to enhance the host immune responses against bacterial diseases.</p

    Internet of Things in Sustainable Energy Systems

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    Our planet has abundant renewable and conventional energy resources but technological capability and capacity gaps coupled with water-energy needs limit the benefits of these resources to citizens. Through IoT technology solutions and state-of-the-art IoT sensing and communications approaches, the sustainable energy-related research and innovation can bring a revolution in this area. Moreover, by the leveraging current infrastructure, including renewable energy technologies, microgrids, and power-to-gas (P2G) hydrogen systems, the Internet of Things in sustainable energy systems can address challenges in energy security to the community, with a minimal trade-off to environment and culture. In this chapter, the IoT in sustainable energy systems approaches, methodologies, scenarios, and tools is presented with a detailed discussion of different sensing and communications techniques. This IoT approach in energy systems is envisioned to enhance the bidirectional interchange of network services in grid by using Internet of Things in grid that will result in enhanced system resilience, reliable data flow, and connectivity optimization. Moreover, the sustainable energy IoT research challenges and innovation opportunities are also discussed to address the complex energy needs of our community and promote a strong energy sector economy

    Chemical Derivatization Processes Applied to Amine Determination in Samples of Different Matrix Composition

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    Analysis of the impact of sex and age on the variation in the prevalence of antinuclear autoantibodies in Polish population: a nationwide observational, cross-sectional study

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    The detection of antinuclear autoantibody (ANA) is dependent on many factors and varies between the populations. The aim of the study was first to assess the prevalence of ANA in the Polish adult population depending on age, sex and the cutoff threshold used for the results obtained. Second, we estimated the occurrence of individual types of ANA-staining patterns. We tested 1731 patient samples using commercially available IIFA using two cutoff thresholds of 1:100 and 1:160. We found ANA in 260 participants (15.0%), but the percentage of positive results strongly depended on the cutoff level. For a cutoff threshold 1:100, the positive population was 19.5% and for the 1:160 cutoff threshold, it was 11.7%. The most prevalent ANA-staining pattern was AC-2 Dense Fine speckled (50%), followed by AC-21 Reticular/AMA (14.38%) ANA more common in women (72%); 64% of ANA-positive patients were over 50 years of age. ANA prevalence in the Polish population is at a level observed in other highly developed countries and is more prevalent in women and elderly individuals. To reduce the number of positive results released, we suggest that Polish laboratories should set 1:160 as the cutoff threshold

    Optical Properties of Zn1x\text{}_{1-x}Mgx\text{}_{x}Se Epilayers Studied by Spectroscopy Methods

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    Linear optical properties of the Zn1x\text{}_{1-x}Mgx\text{}_{x}Se (0 ≤ x ≤ 0.4) alloys have been studied using reflectance, spectroscopic ellipsometry and photoluminescence measurements. The refractive indices of Zn1x\text{}_{1-x}Mgx\text{}_{x}Se epilayers were investigated as a function of Mg composition (0 ≤ x ≤ 0.4). The energies of band gap Eg\text{}_{g} and spin-orbit splitting Eg\text{}_{g}+Δ, have been determined. These energies are shifted gradually to higher values with increasing Mg content

    Characteristics of ADI Ductile Cast Iron with Single Addition of 1.56% Ni

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    The results of examinations of microstructure and an analysis of its impact on selected mechanical properties of austempered ductile iron (ADI) were presented in the paper. The ADI was produced from the ductile iron containing 1.56% Ni only alloying addition. The effect of the austempering time and temperature on the microstructure and mechanical properties of the examined cast iron was considered. Constant conditions of austenitizing were assumed and six variants of the austempering treatment were adopted. The studyof mechanical properties included a static tensile test, Charpy impact strength test and Brinellhardness measurement
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