370 research outputs found
Integrated context-aware and cloud-based adaptive home screens for android phones
This is the post-print version of this Article. The official published version can be accessed from the link below - Copyright @ 2011 Springer VerlagThe home screen in Android phones is a highly customizable user interface where the users can add and remove widgets and icons for launching applications. This customization is currently done on the mobile device itself and will only create static content. Our work takes the concept of Android home screen [3] one step further and adds flexibility to the user interface by making it context-aware and integrated with the cloud. Overall results indicated that the users have a strong positive bias towards the application and that the adaptation helped them to tailor the device to their needs by using the different context aware mechanisms
The Online Dissemination of Nature–Health Concepts: Lessons from Sentiment Analysis of Social Media Relating to “Nature-Deficit Disorder”
Evidence continues to grow supporting the idea that restorative environments, green exercise, and nature-based activities positively impact human health. Nature-deficit disorder, a journalistic term proposed to describe the ill effects of people's alienation from nature, is not yet formally recognized as a medical diagnosis. However, over the past decade, the phrase has been enthusiastically taken up by some segments of the lay public. Social media, such as Twitter, with its opportunities to gather "big data" related to public opinions, offers a medium for exploring the discourse and dissemination around nature-deficit disorder and other nature-health concepts. In this paper, we report our experience of collecting more than 175,000 tweets, applying sentiment analysis to measure positive, neutral or negative feelings, and preliminarily mapping the impact on dissemination. Sentiment analysis is currently used to investigate the repercussions of events in social networks, scrutinize opinions about products and services, and understand various aspects of the communication in Web-based communities. Based on a comparison of nature-deficit-disorder "hashtags" and more generic nature hashtags, we make recommendations for the better dissemination of public health messages through changes to the framing of messages. We show the potential of Twitter to aid in better understanding the impact of the natural environment on human health and wellbeing.Tim Taylor and Marco Palomino acknowledge funding from the European Centre for
Environment and Human Health, part of the University of Exeter Medical School, which was partially financed
by the European Regional Development Fund Programme 2007 to 2013 and European Social Fund Convergence
Programme for Cornwall and the Isles of Scilly during the course of this study. Funding for Sara Warber was
provided in part through a UK-US Fulbright Commission Scholarship that supported her studies at the European
Centre for Environment and Human Health, University of Exeter Medical School
Molecular Taxonomy of Phytopathogenic Fungi: A Case Study in Peronospora
Background: Inappropriate taxon definitions may have severe consequences in many areas. For instance, biologically
sensible species delimitation of plant pathogens is crucial for measures such as plant protection or biological control and for
comparative studies involving model organisms. However, delimiting species is challenging in the case of organisms for
which often only molecular data are available, such as prokaryotes, fungi, and many unicellular eukaryotes. Even in the case
of organisms with well-established morphological characteristics, molecular taxonomy is often necessary to emend current
taxonomic concepts and to analyze DNA sequences directly sampled from the environment. Typically, for this purpose
clustering approaches to delineate molecular operational taxonomic units have been applied using arbitrary choices
regarding the distance threshold values, and the clustering algorithms.
Methodology: Here, we report on a clustering optimization method to establish a molecular taxonomy of Peronospora
based on ITS nrDNA sequences. Peronospora is the largest genus within the downy mildews, which are obligate parasites of
higher plants, and includes various economically important pathogens. The method determines the distance function and
clustering setting that result in an optimal agreement with selected reference data. Optimization was based on both
taxonomy-based and host-based reference information, yielding the same outcome. Resampling and permutation methods
indicate that the method is robust regarding taxon sampling and errors in the reference data. Tests with newly obtained ITS
sequences demonstrate the use of the re-classified dataset in molecular identification of downy mildews.
Conclusions: A corrected taxonomy is provided for all Peronospora ITS sequences contained in public databases. Clustering
optimization appears to be broadly applicable in automated, sequence-based taxonomy. The method connects traditional
and modern taxonomic disciplines by specifically addressing the issue of how to optimally account for both traditional
species concepts and genetic divergence.Peer reviewe
Bridging the Gulf: Phytophthora and Downy Mildews Are Connected by Rare Grass Parasites
Downy mildews and root and foliar rots caused by Phytophthora are among the most destructive plant pathogens and therefore have attracted considerable attention during the past two decades. Although it has been realized that a close phylogenetic relationship exists, so far sharp distinction has been made between the obligate biotrophic downy mildews and the hemibiotrophic Phytophthora. In the study presented here, it is shown that a continuum of character states from hemibiotrophic Phytophthora species to obligate biotrophic downy mildews is present. Intermediate character states between downy mildews and Phytophthora species exist in several rare parasites of grasses, which are not embedded within the major clades of the downy mildews but are placed sister to these, with unresolved affinities to both these clades and to Phytophthora. They still have retained traits hitherto thought to be exclusive for Phytophthora. A careful review of previous research is presented and it is highlighted that uniquely for downy mildews, Poakatesthia may form an intracellular mycelium, growing through several host cells. In addition, scanning electron microscopy reveals that sporangiophore growth is not determinate in Viennotia and that outgrowth from sporangiophores is very similar to Phytophthora infestans. It is concluded that the sharp morphological distinction between downy mildews and Phytophthora species (that are often placed in separate families and even different orders), is rather artificial, since all features thought to be exclusive to Phytophthora or the downy mildews are united in the rare grass-parasitizing down mildew genera Viennotia and Poakatesthia and the enigmatic genus Sclerophthora. Therefore, several paradigms regarding the distinction between Phytophthora and the downy mildews need to be reconsidered
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Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies.
Metagenomic sequence data from defined mock communities is crucial for the assessment of sequencing platform performance and downstream analyses, including assembly, binning and taxonomic assignment. We report a comparison of shotgun metagenome sequencing and assembly metrics of a defined microbial mock community using the Oxford Nanopore Technologies (ONT) MinION, PacBio and Illumina sequencing platforms. Our synthetic microbial community BMock12 consists of 12 bacterial strains with genome sizes spanning 3.2-7.2 Mbp, 40-73% GC content, and 1.5-7.3% repeats. Size selection of both PacBio and ONT sequencing libraries prior to sequencing was essential to yield comparable relative abundances of organisms among all sequencing technologies. While the Illumina-based metagenome assembly yielded good coverage with few misassemblies, contiguity was greatly improved by both, Illumina + ONT and Illumina + PacBio hybrid assemblies but increased misassemblies, most notably in genomes with high sequence similarity to each other. Our resulting datasets allow evaluation and benchmarking of bioinformatics software on Illumina, PacBio and ONT platforms in parallel
Proposals to revise the statutes of the International Committee on Systematics of Prokaryotes
The International Committee on Systematics of Prokaryotes serves to administer the rules of prokaryotic nomenclature via the International Code of Nomenclature of Prokaryotes, ensures the publication of the International Journal of Sys-tematic and Evolutionary Microbiology, and works to represent the interests of the microbiological disciplines regarding prokaryotic nomenclature. The functions and mechanisms of operation of the International Committee on Systematics of Prokaryotes (ICSP) are defined in its Statutes, which were last revised in 2019. As members of the 2020–2023 and the 2023–2026 ICSP Executive Board and the Judicial Commission, we propose here some further revisions to help improve the clarity and functionality of the Statutes.info:eu-repo/semantics/publishedVersio
Toward a Novel Multilocus Phylogenetic Taxonomy for the Dermatophytes.
Type and reference strains of members of the onygenalean family Arthrodermataceae have been sequenced for rDNA ITS and partial LSU, the ribosomal 60S protein, and fragments of β-tubulin and translation elongation factor 3. The resulting phylogenetic trees showed a large degree of correspondence, and topologies matched those of earlier published phylogenies demonstrating that the phylogenetic representation of dermatophytes and dermatophyte-like fungi has reached an acceptable level of stability. All trees showed Trichophyton to be polyphyletic. In the present paper, Trichophyton is restricted to mainly the derived clade, resulting in classification of nearly all anthropophilic dermatophytes in Trichophyton and Epidermophyton, along with some zoophilic species that regularly infect humans. Microsporum is restricted to some species around M. canis, while the geophilic species and zoophilic species that are more remote from the human sphere are divided over Arthroderma, Lophophyton and Nannizzia. A new genus Guarromyces is proposed for Keratinomyces ceretanicus. Thirteen new combinations are proposed; in an overview of all described species it is noted that the largest number of novelties was introduced during the decades 1920-1940, when morphological characters were used in addition to clinical features. Species are neo- or epi-typified where necessary, which was the case in Arthroderma curreyi, Epidermophyton floccosum, Lophophyton gallinae, Trichophyton equinum, T. mentagrophytes, T. quinckeanum, T. schoenleinii, T. soudanense, and T. verrucosum. In the newly proposed taxonomy, Trichophyton contains 16 species, Epidermophyton one species, Nannizzia 9 species, Microsporum 3 species, Lophophyton 1 species, Arthroderma 21 species and Ctenomyces 1 species, but more detailed studies remain needed to establish species borderlines. Each species now has a single valid name. Two new genera are introduced: Guarromyces and Paraphyton. The number of genera has increased, but species that are relevant to routine diagnostics now belong to smaller groups, which enhances their identification
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