evaluation of Lathyrus sativus cultivated in Ethiopia for proximate composition, minerals, β-ODAP and anti-nutritional components

Grass pea (Lathyrus sativus L.) is one of the most important food legumes in  countries like Bangladesh, India and Ethiopia. The legume is nutritious, rich in protein (28-32%) and contains good quantities of essential amino acids. Consumption of Lathyrus sativus seeds has been associated for more than 2000 years with neurolathyrim caused by the nearotoxin ß-ODAP (ß-N-Oxalyl-L-α, ßdiaminopropionic acid) present in the seeds. In the present study, Lathyrus  sativus seed samples  collected from the traditional grass pea growing areas in the country were analyzed for characteristics such as crude protein (CP), ß-ODAP, catechin equivalents (CE), trypsin inhibitor activity (TIA), 100 seed weight, seed color and consumption habit of grass pea seeds. The CP content ranged from 272.9 g/kg -1 to 319.8 g/kg -1 dry matter, the lowest and highest CP being observed in samples collected from Grar Jarso and Akaki, respectively. There were higher variations between samples in ß-ODAP levels. On average, ß-ODAP level in  samples from Akaki was about twofold higher than those from Asgido. CE, which detects simple flavonoids as well as condensed tannins, was not more than 7.66 g/kg-1 dry matter in any sample. The TIA content did not differ significantly between the samples. The correlation coefficients indicated that, ß-ODAP, CE, TIA and 100 seed weight were positively correlated (p<0.05) with CP. In contrast, CE and TIA were negatively correlated (p<0.05) with ß-ODAP. The dietary interviews revealed that grass pea has diversified uses in Ethiopia both as human food and  feed or crop. The habit of consuming shiro wot (the Ethiopian traditional spiced soup-like dish), kik wot (sauce made of dehusked split seeds) kollo (roasted whole seeds), nifro (boiled whole seeds) or kitta (unlearned flat bread) prepared from grass pea alone is significantly higher (p<0.05) than the habit of consuming those food items prepared from grass pea mixed with other legumes. Further studies are  warranted to understand the reasons that influenced food choices of the housewives for establishing respective ways to improve healthy consumption habits.Key words: consumption habit, Lathyrus sativus, ß-ODAP, tannins, trypsin inhibitor activity. Le petit pois (Lathyrus sativus L.) est l’une des légumineuses alimentaires les plus importantes dans des pays tels que le Bangladesh, l’Inde et l’Ethiopie. Les légumineuses sont nutritives, riches en protéines (28-32%) et elles contiennent de  bonnes quantités d’acides aminés essentiels. La consommation des graines de Lathyrus sativus a été associée pendant plus de 2000 ans au neurolathyrime causé par le néarotoxine ß-ODAP (ß-N-Oxalyl-L-α, l’acide ß- diaminopropionique) présentdans les graines. Au cours de la présente étude, des échantillons de graines de Lathyrus sativus collectés à partir des  régions où pousse le petit pois traditionnel dans le pays ont été analysés pour trouver des caractéristiques telles que les   protéines à l’état brut (CP), ß-ODAP, des équivalents de catéchine (CE), l’activité inhibitrice de trypsine (AIT), le poids de la graine 100, la couleur de la graine et les habitudes liées à la consommation des graines de petits pois. Le contenu de CP variait entre 272.9 g/kg -1 et 319.8 g/kg -1 de matière sèche, le niveau le plus bas et le plus élevé de CP étant observés dans des échantillons collectés à Grar Jarso et à Akaki, respectivement. Il y avait des variations plus élevées entre les    échantillons dans les niveaux de ß-ODAP. En moyenne, la teneur en ß-ODAP dans les échantillons d’Akaki était presque le double de celle des échantillons d’Asgido. CE, qui détecte de simples flavonoïdes et des tanins condensés, ne constituait pas plus de 7.66 g/kg-1 de matière sèche dans n’importe quel échantillon. La teneur en AIT ne différait pas beaucoup entre les échantillons. Les coefficients des  corrélations indiquaient que les poids des graines de ß-ODAP, CE, AIT et 100 avaient des corrélations positives (p<0.05) avec CP. Par contre, CE et AIT avaient une corrélation négative (p<0.05) avec ß-ODAP. Les interviews sur les régimes alimentaires ont révélé qu’en Ethiopie le petit pois est utilisé de manières  diversifiées à la fois en tant qu’aliment humain et en tant que ration de nourriture ou culture. L’habitude de consommer le shiro wot (un plat   traditionnel éthiopien épicé qui ressemble à de la soupe), le kik wot (sauce faite de graines cassées et décortiquées), le kollo (des graines entières rôties), le nifro (des graines entières bouillies) ou le kitta (un pain plat désappris), préparés à partir de petit pois  uniquement, est beaucoup plus répandue (p<0.05) que l’habitude de consommer ces denrées alimentaires  préparées à partir du petit pois mélangé avec d’autres légumineuses. Des études plus approfondies s’imposent pour   comprendre les  raisons qui ont influencé les choix des aliments chez les femmes au foyer pour établir les moyens respectifs d’améliorer des habitudes alimentaires saines.Mots-clés: habitude alimentaire, Lathyrus sativus, ß-ODAP, tanins, activité inhibitrice de trypsine.

Vitamin A deficiency status in Tigray Region, Ethiopia, 1996

Abstract: A survey was conducted to determine the prevalence of xerophthalmia in Alaje and Samre weredas of Tigray Region, Northern Ethiopia, where EPI-plus and Wereda Integrated Basic Service (WIBS) approaches are being launched to prevent and control vitamin A deficiency. A total of 5,253 preschool children (PSC) were clinically examined between October and November, 1996 for ocular signs of xerophthalmia. Blood samples were drawn from 248 PSC for serum retinol levels(SRL). The overall prevalence rates of night blindness (XN) and Bitot's spot (X B) for both weredas were 1 0.9% and 1.5%, respectively, with a higher prevalence rate in males than females (53 vs 26). Alaje wereda(EPI-plus) had XN=21(0.8%) and X B=38(1.4%), and Samre wereda (WIBS) had 1 XN=25(1.0%) and X B=41(1.7%). No sex difference was seen in the prevalence rate of corneal 1 xerosis and keratomalacia (0.4%). The most affected age groups were children between five and six years of age. Both weredas showed the distribution of serum retinol levels to be deficient in 21(16.7%) in Samre and 19(15.5%) in Alaje, and low in 60(47.6%) in Samre and 57(46.7%) in Alaje. Low SRL is found to be highest among children between five and six years of age in males and between two to three years of age in females in both weredas. The high prevalence rate of X B 1 (three times higher than the WHO cut-off point), and the low level of serum retinol value found in this study indicates the need and urgency for the continuation of the aforementioned strategies of vitamin A deficiency control program launched in the Region until their impact is further evaluated. [Ethiop. J. Health Dev. 1999;13(2):87-91

Genetic Structure of Modern Durum Wheat Cultivars and Mediterranean Landraces Matches with Their Agronomic Performance

A collection of 172 durum wheat landraces from 21 Mediterranean countries and 20 modern cultivars were phenotyped in 6 environments for 14 traits including phenology, biomass, yield and yield components. The genetic structure of the collection was ascertained with 44 simple sequence repeat markers that identified 448 alleles, 226 of them with a frequency lower than 5%, and 10 alleles per locus on average. In the modern cultivars all the alleles were fixed in 59% of the markers. Total genetic diversity was HT = 0.7080 and the genetic differentiation value was GST = 0.1730. STRUCTURE software allocated 90.1% of the accessions in five subpopulations, one including all modern cultivars, and the four containing landrace related to their geographic origin: eastern Mediterranean, eastern Balkans and Turkey, western Balkans and Egypt, and western Mediterranean. Mean yield of subpopulations ranged from 2.6 t ha-1 for the western Balkan and Egyptian landraces to 4.0 t ha-1 for modern cultivars, with the remaining three subpopulations showing similar values of 3.1 t ha-1. Modern cultivars had the highest number of grains m-2 and harvest index, and the shortest cycle length. The diversity was lowest in modern cultivars (HT = 0.4835) and highest in landraces from the western Balkans and Egypt (HT = 0.6979). Genetic diversity and AMOVA indicated that variability between subpopulations was much lower (17%) than variability within them (83%), though all subpopulations had similar biomass values in all growth stages. A dendrogram based on simple sequence repeat data matched with the clusters obtained by STRUCTURE, improving this classification for some accessions that have a large admixture. landraces included in the subpopulation from the eastern Balkans and Turkey were separated into two branches in the dendrogram drawn with phenotypic data, suggesting a different origin for the landraces collected in Serbia and Macedonia. The current study shows a reliable relationship between genetic and phenotypic population structures, and the connection of both with the geographic origin of the landraces.The research was funded by the Ministerio de Economía y competitividad project AGL-2006-09226-C02-01, and Dr. Jose Miguel Soriano is funded by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (http://www.mineco.gob.es/)

Geographic distribution of MERS coronavirus among dromedary camels, Africa

We found serologic evidence for the circulation of Middle East respiratory syndrome coronavirus among dromedary camels in Nigeria, Tunisia, and Ethiopia. Circulation of the virus among dromedaries across broad areas of Africa may indicate that this disease is currently underdiagnosed in humans outside the Arabian Peninsula

Content of zinc, iron, calcium and their absorption inhibitors in foods commonly consumed in Ethiopia

The zinc, iron, calcium, phosphorus, phytate, tannin and moisture content of 36 foods consumed in rural Ethiopia were analysed. The foods analysed included those based on cereals, starchy tubers and roots, and legumes and vegetables as well as some fruits. Although many foods were relatively rich in zinc and iron, many also contained high levels of phytic acid and tannins, which impair bioavailability of zinc and iron. The phytate:zinc molar ratios were >20 for non-fermented cereal foods, >15 for legumes, and 15 are associated with low bioavailability of zinc. Given the high iron content and the relatively favourable phytate:iron molar ratio, tef enjera was the best source of bioavailable iron of all foods analysed. Foods prepared from tef, enset and kale are rich sources of calcium. The consumption of diets based on cereals and legumes but poor in animal products can lead to deficiencies of zinc and iron. However, since fermentation can decrease the phytate content by a factor of 3¿4, traditional household practices such as fermentation need to be encouraged to address the problem of zinc deficiency, which is particularly prevalent in Ethiopi

PEER REVIEWED ARTICLE No 10 - EVALUATION OF LATHYRUS SATIVUS CULTIVATED IN ETHIOPIA FOR PROXIMATE COMPOSITION, MINERALS, â-ODAP AND ANTI-NUTRITIONAL COMPONENTS

Grass pea (Lathyrus sativus L.) is one of the most important food legumes in countries like Bangladesh, India and Ethiopia. The legume is nutritious, rich in protein (28-32%) and contains good quantities of essential amino acids. Consumption of Lathyrus sativus seeds has been associated for more than 2000 years with neurolathyrim caused by the nearotoxin β-ODAP (β-N-Oxalyl-L-α, βdiaminopropionic acid) present in the seeds. In the present study, Lathyrus sativus seed samples collected from the traditional grass pea growing areas in the country were analyzed for characteristics such as crude protein (CP), β-ODAP, catechin equivalents (CE), trypsin inhibitor activity (TIA), 100 seed weight, seed color and consumption habit of grass pea seeds. The CP content ranged from 272.9 g/kg -1 to 319.8 g/kg -1 dry matter, the lowest and highest CP being observed in samples collected from Grar Jarso and Akaki, respectively. There were higher variations between samples in β-ODAP levels. On average, β-ODAP level in samples from Akaki was about twofold higher than those from Asgido. CE, which detects simple flavonoids as well as condensed tannins, was not more than 7.66 g/kg-1 dry matter in any sample. The TIA content did not differ significantly between the samples. The correlation coefficients indicated that, β-ODAP, CE, TIA and 100 seed weight were positively correlated (p<0.05) with CP. In contrast, CE and TIA were negatively correlated (p<0.05) with β-ODAP. The dietary interviews revealed that grass pea has diversified uses in Ethiopia both as human food and feed or crop. The habit of consuming shiro wot (the Ethiopian traditional spiced soup-like dish), ≤kik wot (sauce made of dehusked split seeds) kollo (roasted whole seeds), nifro (boiled whole seeds) or kitta (unlearned flat bread) prepared from grass pea alone is significantly higher (p<0.05) than the habit of consuming those food items prepared from grass pea mixed with other legumes. Further studies are warranted to understand the reasons that influenced food choices of the housewives for establishing respective ways to improve healthy consumption habits

Effects of blanching and soaking on some physical characteristics of grass pea (Lathyrus sativus)

Lathyrus sativus, containing a lathyritic principle, called &#946;-ODAP (&#946;-N-Oxalyl-L- &#945;, &#946;-diaminopropionic acid), is widely grown and consumed in Ethiopia. It is a hardy crop grown under various agro-ecological situations. The major drawback in the use of the legume is the fact that the seeds, in common with other legumes, are not easily rehydrated, are difficult to cook, the seed coat is difficult to remove and long cooking time is required by traditional processing methods for tenderization. In this study, the effect of processing methods like blanching, soaking and cooking on water absorption, leached solids, swelling power, cooking time and sensory qualities of grass pea seeds were investigated. The soaking solutions used were mixed with salt solution, wood ash solution and double distilled de-ionized water. Blanching significantly (p < 0.05) increased total water absorbed, swelling power, leached solids and reduced cooking time compared to unbalanced seeds. Soaking blanched seeds in salt or wood ash solution was noted to be most effective in promoting seed softening during cooking, compared to double distilled water. Blanched grass pea seeds soaked for 12 hours in the three soaking solutions caused reduction of 60%, 73% and 68%, respectively, in cooking time. In contrast, unblanched grass pea seeds soaked for 12 hours in the three soaking solutions caused reduction of 30%, 46% and 40%, respectively, in cooking time. Water absorption, swelling power and leached solids values were negatively correlated (p < 0.05) with the cooking time. The study results indicated that blanching and hydration of grass pea seeds improved the sensory characteristics of cooked seeds, significantly reduced the cooking time and increased the weight and texture of the cooked seeds. Application of blanching preceded by soaking at village level as an integral part of traditional methods of processing grass pea seeds, thus offers the dual advantage of saving of valuable fuels by shortening cooking time, as well as rendering the seeds more acceptable to consumers. In the absence of salt, wood ash suspension treatment may be a satisfactory alternative in the production of quick-cooking grass pea seeds. Keywords: blanching, cooking, Lathyrus sativus, soaking African Journal of Food, Agriculture, Nutrition and Development Vol. 6(1) 200