Notes on Aquarium Brood Release and Feeding of the Opossum Pipefish, Microphis brachyurus lineatus

The opossum pipefish, Microphis brachyurus lineatus, is a migratory syngnathid with a salmon-like life history (Frias-Torres 2002). Adults breed in freshwater and oligohaline habitats associated with emergent vegetation, mostly Panicum sp. and Polygonum sp.; breeding occurs during the wet season (May to November) (Gilmore and Hastings 1983). In southern Mexico, opossum pipefish associate with Ruppia maritima, and reproduction is year-round (Miranda-Marure et al. 2004). Pelagic juveniles are found in the Atlantic Ocean and are associated with Sargassum sp. (Böhlke and Chaplin 1968). Juveniles eventually recruit into oligohaline habitats. The species range is from Sao Paulo, Brazil, to New Jersey, USA, although the northernmost limit of permanent breeding populations occurs in the freshwater tributaries to the Indian River Lagoon in east central Florida (Gilmore and Gilbert 1992). The opossum pipefish is one of 25 species of diadromous fish at risk of extinction in North America (Musick et al. 2000) and is a candidate species to the US Threatened and Endangered Species List (Federal Register 1999). The opossum pipefish diet was unknown until Teixeira and Perrone (1998) analyzed the gut contents of 109 opossum pipefish from the southeastern Brazil population. They found that the most abundant prey were insect larvae, followed by juvenile fish (Gerreidae) and small crustaceans. However, the diet necessary to maintain aquarium-kept opossum pipefish had not been determined prior to the present paper

Caracterización del microbioma del ciego en cuyes (Cavia porcellus) de las razas Inti, Perú y Andina, Chachapoyas-2021

El cuy (Cavia porcellus) es un roedor de alta importancia económica y nutricional con creciente demanda y poca oferta en el mercado nacional. Existiendo una escasa aplicación de biotecnologías que incrementen eficiencia de producción y mejoramiento en la calidad cárnica. La fisiología digestiva de fermentación bacteriana post-gástrica que ocurre dentro del ciego, donde existe un consorcio bacteriano (el microbioma) especializado en la degradación de fibra, asociado a la absorción de nutrientes. El microbioma tiene un papel importante propia de cada raza asociado a su fenotipo, en ese contexto. El objetivo de este estudio fue caracterizar el microbioma de ciego de cuyes de las razas Inti, Perú y Andina mediante metagenómica para identificar posibles microorganismos asociados a la degradación y absorción de nutrientes. Fueron diseñados dos tratamientos de acuerdo a la raza y el suministro de alimento previo al sacrificio (animales con ayuno de 24 horas y sin ayuno). Así mismo se realizó el secuenciamiento de las regiones hipervariables V3-V4 del gen 16S ARNr al cual se aplicó un análisis bioinformático mediante el paquete QIIME2-2022.2. Encontrando más de 180 secuencias por muestra, de las cuales el 58.37% pertenecían al Phylum Firmicutes y el 27.99% al Phylum Bacteroidetes. No se encontraron diferencias en la composición bacteriana entre las 3 razas estudiadas, sin embargo, se vio que el ayuno por 24 horas tiene un efecto en la diversidad microbiana. En consecuencia, el ciego de cuy está dominado por bacterias pertenecientes a los Phylum (Firmicutes y Bacteroidetes), y a nivel de género (Ruminococcus, Prevotella y Fibrobacter) y especies (Ruminococcus flavefaciens, Ruminococcus albus, Ruminococcus bromii, Prevotella ruminicola y Fibrobacter succinogenes), dichas bacterias implicadas en la degradación de la fibra, candidatos potenciales para usó como probióticos en la producción sostenible del cuy

Simulación Monte Carlo para estimar la cantidad a producir de estilos de trajes de baño en una empresa de confecciones

El documento digital no refiere un asesorPublicación a texto completo no autorizada por el autorMuestra las posibilidades extremas - los resultados de tomar la medida más arriesgada y la más conservadora - así como todas las posibles consecuencias de las decisiones intermedias, lo cual se ejemplificará mediante el desarrollo de la aplicación práctica en el caso de la empresa de confecciones de trajes de baño Applauzi S.A. Esta aplicación muestra a la Simulación Monte Carlo como herramienta para sugerir a los decisores la mejor alternativa para establecer la cantidad a producir de determinado producto.Trabajo de suficiencia profesiona

Flooding through the lens of mobile phone activity

Natural disasters affect hundreds of millions of people worldwide every year. Emergency response efforts depend upon the availability of timely information, such as information concerning the movements of affected populations. The analysis of aggregated and anonymized Call Detail Records (CDR) captured from the mobile phone infrastructure provides new possibilities to characterize human behavior during critical events. In this work, we investigate the viability of using CDR data combined with other sources of information to characterize the floods that occurred in Tabasco, Mexico in 2009. An impact map has been reconstructed using Landsat-7 images to identify the floods. Within this frame, the underlying communication activity signals in the CDR data have been analyzed and compared against rainfall levels extracted from data of the NASA-TRMM project. The variations in the number of active phones connected to each cell tower reveal abnormal activity patterns in the most affected locations during and after the floods that could be used as signatures of the floods - both in terms of infrastructure impact assessment and population information awareness. The representativeness of the analysis has been assessed using census data and civil protection records. While a more extensive validation is required, these early results suggest high potential in using cell tower activity information to improve early warning and emergency management mechanisms.Comment: Submitted to IEEE Global Humanitarian Technologies Conference (GHTC) 201

Cox Models and Cure Mixture Models

Introduction : Ulcerative colitis (UC) is an idiopathic chronic inflammatory bowel disease characterized by periods of remission and acute exacerbations, and unpredictable disease course. Despite the availability of new drugs, there are still a considerable proportion of patients that will be refractory to therapy, need colectomy or develop complications, highlighting the need for better predictive markers of disease course. Considering the endoscopic extent of disease at the time of diagnosis, patients were divided into three major subgroups: inflammation limited to the rectum (E1), left-sided colitis with inflammation distally to the splenic flexure (E2) and extensive colitis with involvement proximal to the splenic flexure (E3). The combination of endoscopy and histology seem to provide a better indication of disease activity than endoscopy alone, especially in endoscopically non-inflamed mucosa. No study has assessed the prognostic value of histological features at diagnosis, and therefore it remains unknown whether the pre-treatment microscopic findings can predict the course of the disease. Herein, we designed a multicenter retrospective study to evaluate the prognostic value of histologic features in treatment-naïve proctitis and left-sided ulcerative colitis patients. Our goals were to evaluate the prevalence of microscopic inflammation in the macroscopically uninflamed mucosa, as well as the predictive value of the histological features of disease (in the endoscopically inflamed and non-inflamed mucosa) at the time of diagnosis. In Portugal there has been a considerable consumption of medical resources associated to UC.  Between 2010 and 2017, around 3400 people were hospitalized due to this disease, with an average hospital stay of 10 days with a minimum of 0 days and a maximum of 109 days, making the study of hospitalizations in this disease is a very relevant issue in order to have a perspective of its occurrence, as well as to help in the monitoring of patients and in clinical and hospital administration decision making. In this work, we analyse the time to the need of hospitalization of 93 patients diagnosed with UC in stages E1 and E2. Methods: Cox models were used since proportional hazards assumption was not rejected and Cox models are popular amongst medical researchers, especially concerning the parameter interpretation. Moreover, according to Nardi and Scheper (2003), a good discrimination among parametric models require censoring not to exceed 40-50 per cent. Our data is of moderate size and about 85% of the cases correspond to censored times. Nevertheless, for comparison purposes, we consider fitting parametric models in future work since they can perform better than the Cox model if the conditions they require are met. Results: Age, endoscopic extent and severity scores at diagnosis on the time to hospitalization were the considered covariates. From these, only age was significantly associated, with older individuals being expected to have later hospitalizations (HR 0.95 (CI 95%: 0.9-0.99), p-value=0.016). As said above, a large proportion of individuals had no hospitalizations despite their long disease history. The observed mean time to hospitalization was 1.34 years (0.02 - 3.31 years) whereas the average time of censured observations was 4.33 years (0.18 - 26.95 years), suggesting that there are individuals who will never experience the event of interest, even if the time under observation could be prolonged indefinitely. Therefore, we considered the possible existence of “healed to hospitalization” individuals. To explore this hypothesis, cure models were implemented considering the same set of covariates for comparison purposes. It is assumed that the individuals come from a mixture of two populations – those who will experience a hospitalization and those who will not. Besides producing an estimate of the percentage of the “cured” population, it is expected to obtain a more realistic estimate of the survival function for the fraction of the population who will suffer the event. The estimated cure rate is 30.5 percent, meaning that it is expected that about 30.5% of the censored individuals will never experience hospitalization. In the component defining the cure and not cure, the only significant at 10% was variable was nancy score (OR=4.36; CI90%: 1.09-17.4; p-value=0.08), with higher values of Nancy score favouring susceptibility. Regarding the survival of susceptible individuals, none of the variables under analysis were significant. Discussion and Conclusions: Comparing the results of both models made us believe that to disregard the possibility of “cure” may lead to biased conclusions concerning the influence of the considered covariates in the time to hospitalization

Piezoelectric bone stimulation: should we move closer to nature?

The aim of this work was to experimentally validate the use of piezoelectric materials as a mean of directly straining bone cells by converse piezoelectric effect

Asymptotic properties of parameter estimates for random fields with tapered data

In this paper we present novel results on the asymptotic be-havior of the so-called Ibragimov minimum contrast estimates. The case of tapered data for various models of Gaussian random fields is investigated. MSC 2010 subject classifications: Primary 62F12, 62M30; secondary 60G60

Metastatic Intraocular Tumor Due to Colorectal Adenocarcinoma: Case Report and Literature Review

Purpose: To describe the clinical and histopathological findings of a case of intraocular metastasis due to colorectal adenocarcinoma and to carry out a literature review. Case Report: A 64-year-old man with a history of tumor resection due to infiltrating colorectal adenocarcinoma three years previously sought ophthalmological care because of severe ocular pain without response to medical treatment and progressive vision loss in the left eye. On ultrasonographic examination, there was a heterogeneous intraocular choroidal tumor, which occupied approximately 40% of the vitreous cavity, as well as peritumoral serous retinal detachment. The patient underwent left eyeball enucleation. The histopathological diagnosis was metastatic tubular adenocarcinoma involving the retina and choroid that partially infiltrated the sclera and the proximal optic nerve. Conclusion: The present case highlights a rare pathological entity associated with variable therapeutic schemes and survival times and poor prognosis in patients with metastatic intraocular tumors due to colorectal adenocarcinoma

Dental Caries And Treatment Needs In Adolescents From The State Of Sao Paulo, Brazil

Objective: To estimate the prevalence of dental caries and treatment needs in 12-year-olds and adolescents. Methods: Cross-sectional study based on results from the epidemiological surveys: Oral Health Conditions in the State of Sao Paulo, 2002 and the Brazilian Oral Health Survey (SBBrasil) 2010. Secondary data for 5,782 (2002) and 369 (2010) 12-year-olds and 880 (2002) and 300 (2010) 15-to 19-year-olds were analyzed. Dental caries attack was evaluated using the DMFT (decayed, missing or fi lled teeth) index and the need for treatment verifi ed using the criteria proposed by the World Health Organization. The Signifi cant Caries Index was used to measure the severity of the decay in the tercile of the group with the highest prevalence of the disease. In order to analyze the results, the Chi-squared and Mann-Whitney tests were used, with a 5% signifi cance. Results: There was a decrease of 39.3 percentage points in the DMFT index for 12-year-olds (p < 0.001) and of 41.1 percentage points for the adolescents (p < 0.001) between 2002 and 2010, and an increase of around 161.0 and 303.0 percentage in the group which was free from dental caries respectively. The percentage of restored teeth decreased in both age groups, although the prevalence of dental caries did not change in the group more affected by dental caries. In the group with few dental caries, there was a decrease in the component 'tooth loss' for adolescents and increase in the component 'decayed teeth' for the 12-yearsold and the adolescents. There was an increase in the need for dental treatment in the group as a whole and in the group of 12-year-olds more affected by dental caries; and among the adolescents, the need for restoration on two or more surface decreased in the group as a whole and also in the group which suffered least from dental caries. Conclusions: The decreasing need for non-complex treatment in adolescents suggests that promotion and prevention activities are having a positive effect on this group. Moreover, the two epidemiological surveys in the state of Sao Paulo show improvements in oral health conditions in both age groups studied and calls for monitoring aimed also at the group least affected by dental caries.47SUPPL.35058Al-Haddad, K.A., Al-Hebshi, N.N., Ak'hali, M.S., Oral health status and treatment needs among school children in Sana'a City, Yemen (2010) Int J Dent Hyg., 8 (2), pp. 80-85. , DOI: 10.1111/j.1601-5037.2009.00398.xArmfield, J.M., Spencer, A.J., Slade, G.D., Changing inequalities in the distribution of caries associated with improving child oral health in Australia (2009) J Public Health Dent., 69 (2), pp. 125-134. , Pesquisa Nacional de Saúde Bucal (SBBrasil) DOI: 10.1111/j.1752-7325.2008.00110.xBagramian, R.A., Franklin, G.G., Anthony, R.V., The global increase in dental caries (2009) A pending public health crisis. Am J Dent., 22 (1), pp. 3-8Bratthall, D., Introducing the Signifi cant Caries Index together with a proposal for a new global oral health goal for 12-year-olds (2000) Int Dent J., 50 (6), pp. 378-384. , DOI: 10.1111/j.1875-595X.2000.tb00572.xCampus, G., Sacco, G., Cagetti, M., Abati, S., Changing trend of caries from 1989 to 2004 among 12-year old Sardinian children (2007) BMC Public Health., 7, p. 28Constante, H.M., Bastos, J.L., Peres, M.A., Trends in dental caries in 12-and 13-year-old schoolchildren from Florianópolis between 1971 and 2009 (2010) Braz J Oral Sci., 9 (3), pp. 410-414Davies, G.M., Jones, C.M., Monaghan, N., Morgan, M.Z., Neville, J.S., Pitts, N.B., The caries experience of 11 to 12 year-old children in Scotland and Wales and 12 year-olds in England in 2008-2009: reports of co-ordinated surveys using BASCD methodology (2012) Community Dent Health., 29 (1), pp. 8-13. , DOI: 10.1111/j.1471-4159.2012.07745.xDash, J.K., Sahoo, P.K., Bhuyan, S.K., Sahoo, S.K., Prevalence of dental caries and treatment needs among children of Cuttack (Orissa) (2002) J Indian Soc Pedo Prev Dent., 20 (4), pp. 139-143Ditmyer, M., Dounis, G., Mobley, C., Schwarz, E., Inequalities of caries experience in Nevada youth expressed by DMFT index vs (2011) Signifi cant Caries Index (SiC) over time. BMC Oral Health., 11, p. 12. , DOI: 10.1186/1472-6831-11-12Do, L.G., Distribution of caries in children: Variations between and within Populations (2012) J Dent Res., 91 (6), pp. 536-543. , DOI: 10.1177/0022034511434355Duraiswamy, P., Kumar, T.S., Dagli, R.J., Chandrakant, K.S., Dental caries experience and treatment needs of green marble mine laborers in Udaipur district, Rajasthan, India (2008) Indian J Dent Res., 19 (4), pp. 331-334. , DOI: 10.4103/0970-9290.44537Dye, B.A., Arevalo, O., Vargas, C.M., Trends in paediatric dental caries by poverty status in the United States, 1988-1994 and 1999-2004 (2010) Int J Paediatr Dent., 20 (2), pp. 132-143. , DOI: 10.1111/j.1365-263X.2009.01029.xFrias, A.C., Antunes, J.L.F., Narvai, P.C., Precisão e validade de levantamentos epidemiológicos em saúde bucal: cárie dentária na Cidade de São Paulo, 2002 (2004) Rev Bras Epidemiol., 7 (2), pp. 144-154. , DOI: 10.1590/S1415-790X2004000200004Gushi, L.L., Soares, M.C., Forni, T.I.B., Vieira, V., Wada, R.S., Sousa, M.L.R., Cárie dentária em adolescents de 15 a 19 anos de idade no Estado de São Paulo, Brasil, 2002 (2005) Cad Saude Publica., 21 (5), pp. 1383-1391. , DOI: 10.1590/S0102-311X2005000500010Gushi, L.L., Rihs, L.B., Soares, M.C., Forni, T.I.B., Vieira, V., Wada, R.S., Cárie dentária e necessidades de tratamento em adolescentes do estado de São Paulo, 1998 e 2002 (2008) Rev Saude Publica., 42 (3), pp. 480-486. , DOI: 10.1590/S0034-89102008005000015Kulkami, S.S., Deshpande, S.D., Caries prevalence and treatment needs in 11-15 year old children of Belgaum city (2002) J Indian Soc Prev Dent., 20 (1), pp. 12-15Martins, R.J., Garbin, C.A.S., Garbin, A.J.I., Moimaz, S.A.S., Saliba, O., Declínio da cárie em um município da região noroeste do Estado de São Paulo, Brasil, no período de 1998 a 2004 (2006) Cad Saude Publica., 22 (5), pp. 1035-1041. , DOI: 10.1590/S0102-311X2006000500016Mashoto, K.O., Astrom, A.N., Skeie, M.S., Masalu, J.R., Socio-demographic disparity in oral health among the poor: a cross sectional study of early adolescentes in Kilwa district, Tanzania (2010) BMC Oral Health., 10, p. 7. , DOI: 10.1186/1472-6831-10-7Narvai, P.C., Frazão, P., Roncalli, A.G., Antunes, J.L.F., Cárie dentária no Brasil: declínio, iniqüidade e exclusão social (2006) Rev Panam Salud Publica., 19 (6), pp. 385-393. , DOI: 10.1590/S1020-49892006000600004Piovesan, C., Mendes, F.M., Antunes, J.P.F., Ardenghi, T.M., Inequalities in the distribution of dental caries among 12-year-old Brazilian schoolchildren (2011) Braz Oral Res., 25 (1), pp. 69-75. , DOI: 10.1590/S1806-83242011000100012Rihs, L.B., Sousa, M.L.R., Cypriano, S., Abdalla, N.M., Desigualdades na distribuição da cárie dentária em adolescents de Indaiatuba (SP), 2004 (2010) Cienc Saude Coletiva., 15 (4), pp. 2173-2180. , DOI: 10.1590/S1413-81232010000400031Schulte, A.G., Momeni, A., Pieper, K., Caries prevalence in 12-year-old children from Germany (2006) Results of the 2004 national survey. Community Dent Health., 23 (4), pp. 197-202. , DOI: 10.1590/S1413-81232010000400031Steiner, M., Menghini, G., Marthaler, T.M., Imfeld, T., Changes in dental caries in Zurich school-children over a period of 45 years (2010) Schweiz Monatsschr Zahnmed., 120 (12), pp. 1084-1104Tang, J., Yu, Y., Ma, Y., The Epidemic Tendency of Dental Caries Prevalence of School Students from 1991 to 2005 in China (2010) J Huazhong Univ Sci Technol., 30 (1), pp. 132-137. , DOI: 10.1007/s11596-010-0124-2Umesi-Koleoso, D.C., Ayanbadejo, P.O., Oremosu, O.A., Dental caries trend among adolescents in Lagos, Southwest Nigeria (2007) WAJM., 26 (3), pp. 201-205Oral health surveys: basic methods (1997), World Health Organization. 4. ed. Genev

Dental caries and treatment needs in adolescents from the state of Sao Paulo, Brazil

OBJECTIVE: To estimate the prevalence of dental caries and treatment needs in 12-year-olds and adolescents. METHODS: Cross-sectional study based on results from the epidemiological surveys: Oral Health Conditions in the State of Sao Paulo, 2002 and the Brazilian Oral Health Survey (SBBrasil) 2010. Secondary data for 5,782 (2002) and 369 (2010) 12-year-olds and 880 (2002) and 300 (2010) 15- to 19-year-olds were analyzed. Dental caries attack was evaluated using the DMFT (decayed, missing or filled teeth) index and the need for treatment verified using the criteria proposed by the World Health Organization. The Significant Caries Index was used to measure the severity of the decay in the tercile of the group with the highest prevalence of the disease. In order to analyze the results, the Chi-squared and Mann-Whitney tests were used, with a 5% significance. RESULTS: There was a decrease of 39.3 percentage points in the DMFT index for 12-year-olds (p &lt; 0.001) and of 41.1 percentage points for the adolescents (p &lt; 0.001) between 2002 and 2010, and an increase of around 161.0 and 303.0 percentage in the group which was free from dental caries respectively. The percentage of restored teeth decreased in both age groups, although the prevalence of dental caries did not change in the group more affected by dental caries. In the group with few dental caries, there was a decrease in the component &#8216;tooth loss&#8217; for adolescents and increase in the component &#8216;decayed teeth&#8217; for the 12-years-old and the adolescents. There was an increase in the need for dental treatment in the group as a whole and in the group of 12-year-olds more affected by dental caries; and among the adolescents, the need for restoration on two or more surface decreased in the group as a whole and also in the group which suffered least from dental caries. CONCLUSIONS: The decreasing need for non-complex treatment in adolescents suggests that promotion and prevention activities are having a positive effect on this group. Moreover, the two epidemiological surveys in the state of Sao Paulo show improvements in oral health conditions in both age groups studied and calls for monitoring aimed also at the group least affected by dental caries.OBJETIVO: Estimar a prevalência de cárie dentária e necessidades de tratamento em crianças de 12 anos e adolescentes. MÉTODOS: Estudo transversal com base nos resultados dos levantamentos epidemiológicos Condições de Saúde Bucal no Estado de São Paulo em 2002 e Pesquisa Nacional de Saúde Bucal (SBBrasil) 2010. Foram analisados os dados secundários de 5.782 crianças (2002) de 12 anos e outras 369 (2010); e para a faixa de 15 a 19 anos foram analisados 880 jovens (2002) e 300 jovens em 2010. A experiência de cárie foi avaliada pelo índice CPOD (dentes cariados, perdidos e obturados) e foram verificadas as necessidades de tratamento odontológico segundo os critérios propostos pela Organização Mundial da Saúde. O índice Significant Caries Index foi empregado para medir a severidade da cárie no terço do grupo que apresentou maior prevalência da doença. Para a análise dos resultados utilizaram-se os testes de Qui-quadrado e Mann-Whitney, com nível de 5% de significância. RESULTADOS: Houve diminuição de 39,3 pontos percentuais no índice CPOD aos 12 anos (p < 0,001) e de 41,1 pontos percentuais nos adolescentes (p < 0,001) entre 2002 e 2010, e aumento de aproximadamente 161,0 pontos percentuais e 303,0 pontos percentuais no grupo livres de cárie, respectivamente. A porcentagem de dentes restaurados diminuiu nos dois grupos etários, mas a prevalência de dentes cariados não se alterou para o grupo de alta experiência de cárie. No grupo de baixa experiência de cárie ocorreu diminuição do componente perdido para os adolescentes e aumento do componente cariado aos 12 anos e adolescentes. Houve aumento da necessidade de tratamento endodôntico no grupo total e no de alta experiência de cárie aos 12 anos; e entre os adolescentes a necessidade de restauração de duas ou mais faces diminuiu no grupo todo e também no de baixa experiência. CONCLUSÕES: A diminuição da necessidade de tratamento de baixa complexidade entre adolescentes sugere que as ações de promoção e prevenção estão afetando positivamente esse grupo. Além disso, os dois levantamentos epidemiológicos no estado de São Paulo mostram melhorias na condição de saúde bucal dos grupos etários estudados e que se faz necessário o monitoramento direcionado também para o grupo de baixa experiência de cárie.OBJETIVO: Estimar la prevalencia de caries dentaria y necesidades de tratamiento en ni&#241;os de 12 a&#241;os y adolescentes. M&#201;TODOS: Estudio transversal con base en los resultados de las pesquisas epidemiol&#243;gicas de las Condiciones de Salud Bucal en el Estado de Sao Paulo en 2002 y Proyecto SBBrasil2010. Se analizaron los datos secundarios de 5.782 ni&#241;os (2002) de 12 a&#241;os y 369 (2010); y para el grupo etario de 15 a 19 a&#241;os se analizaron 880 j&#243;venes (2002) y 300 j&#243;venes en 2010. La experiencia de caries fue evaluada por el &#237;ndice CPOD (dientes cariados, perdidos y obturados) y se verificaron las necesidades de tratamiento odontol&#243;gico seg&#250;n los criterios propuestos por la Organizaci&#243;n Mundial de la Salud. El &#237;ndice Significant Caries Index fue empleado para medir la severidad de la caries en el tercio del grupo que present&#243; mayor prevalencia de la enfermedad. Para el an&#225;lisis de los resultados se utilizaron las pruebas de Chi-cuadrado y Mann-Whitney, con nivel de 5% de significancia. RESULTADOS: Hubo disminuci&#243;n de 39,3 puntos porcentuales en el &#237;ndice CPOD a los 12 a&#241;os (p&lt;0,001) y de 41,1 puntos porcentuales en los adolescentes (p&lt;0,001) entre 2002 y 2010 y aumento de aproximadamente 161,0 puntos porcentuales y 303,0 puntos porcentuales en el grupo libres de caries, respectivamente. El porcentaje de dientes restaurados disminuy&#243; en los dos grupos etarios, pero la prevalencia de dientes cariados no se alter&#243; para el grupo con alta experiencia de caries. En el grupo con baja experiencia de caries ocurri&#243; disminuci&#243;n del componente perdido para los adolescentes y aumento del componente cariado para los de 12 a&#241;os y adolescentes. Hubo aumento de la necesidad de tratamiento endod&#243;ntico en el grupo total y en el de alta experiencia de caries a los 12 a&#241;os; y entre los adolescentes la necesidad de restauraci&#243;n de dos o m&#225;s faces disminuy&#243; en el grupo todo y tambi&#233;n en el de baja experiencia. CONCLUSIONES: La disminuci&#243;n de la necesidad de tratamiento de baja complejidad entre adolescentes sugiere que las acciones de promoci&#243;n y prevenci&#243;n est&#225;n afectando positivamente este grupo. As&#237; mismo, las dos pesquisas epidemiol&#243;gicas en el estado de Sao Paulo muestran mejor&#237;as en la condici&#243;n de salud bucal de los grupos etarios estudiados y que se hace necesario el monitoreo direccionado para el grupo de baja experiencia de caries.505