Mary E. Frayser Papers - Accession 1

The Mary E. Frayser Papers consist of correspondence, speeches, reports, clippings, minutes, histories, family histories, constitutions and bylaws, membership lists, program notes, photographs, and other papers, relating to her work with the South Carolina Extension Service (1912-1940) Winthrop College, her involvement with the South Carolina Council for the Common Good (1935-1952), the South Carolina Federation of Women’s Clubs (1926-1952), the South Carolina Status of Women Conference (1945-1952), the South Carolina Division of the American Association of University Women (AAUW) (1929, 1935-1949), the South Carolina Interracial Institute (1938-1942), the South Carolina Division of the Southern Regional Council (1944-1951), and the South Carolina Conference of Social Work (1936-1967). There are also papers relating to Frayser’s efforts to promote social and economic legislation and participation by women in public affairs and her interest in libraries and work in the movement for the support of public libraries in South Carolina (1925-1968). Correspondents included G.H. Aault, Evan Chesterman, Wil Lou Gray, Sarah Hughes, Christine South Gee, and Maude Massey Rogers. This collection is a good source of women’s club activities in the twentieth century. Important areas of research would include the way club activity affected social and economic legislation in the state and the various forces involved in the movement for state tax supported libraries. While the papers do range from 1841 to 1953, the greater bulk of the papers extend from the early 1930s to about 1947. Since the work of the various women\u27s club organizations were so inter-related, a researcher working with the papers of a particular organization for a particular time span should consider the Frayser papers of all other organizations. The related papers for the “Correspondence and Related Papers” series for particular organizations are generally similar and include: memoranda, outlines, reports, resolutions, minutes, etc. Additional Frayser information can be found by referring to the Winthrop University Archives (official records).https://digitalcommons.winthrop.edu/manuscriptcollection_findingaids/1009/thumbnail.jp

THE RELATIONSHIP BETWEEN TEACHER BELIEFS ABOUT RESPONSE TO INTERVENTION AND STUDENT OUTCOMES: AN ATTEMPT TO GAIN TEACHER BUY IN AND INCREASE STUDENT OUTCOMES

Response to Intervention (RtI) is a tiered system of supports designed to offer early prevention services, blend general and special education services, focus on research-based strategies, and emphasize data-based decisions. This study examines teacher beliefs about Response to Intervention (RtI) and teachers’ perceptions of the skill set they possess to implement RtI through a survey method. Examining the role of educators in the implementation and sustainment of a mandated initiative is fundamental to successful usage of the framework. Additionally, the study examines the connection between teacher beliefs/skills and student learning outcomes on a reading curriculum-based measurement. A regression analysis is conducted to determine if a relationship exists between teacher beliefs/skills and an increase in student learning. Furthermore, teacher feedback was gathered on how they obtained their skill set and what supports they need in the future from the participating school district. This study found a significant relationship exists between teacher beliefs/skills and student learning. Specifically, teacher beliefs about data-based decision making were found to have a significant relationship with class average student growth rates on a reading curriculum-based growth measurement

Mary Elizabeth Frayser Papers - Accession 351

This collection, primarily correspondence, relates to Mary Elizabeth Frayser’s career, business dealings, and family. Also included are financial records, a biographical sketch of her business partner William Horton, a book titled, Things Mundane which is a collection of humorous observations by “The Idle Reporter,” Evan R. Chesterman, who wrote for the Richmond Evening Journal, newspaper clippings, and photographs. Correspondents include her sister Ellie Douglas Frayser Chesterman (1876-1958) and, Mary’s brother-in-law, Evan Ragland Chesterman, Sr. (1870-1931). Mary Elizabeth Frayser (1868-1968) was a South Carolina educator, social worker, home economist, and club woman.https://digitalcommons.winthrop.edu/manuscriptcollection_findingaids/2744/thumbnail.jp

The Route to Water Security for Texas: The 2015–2016 Texas Water Roadmap Forums

Three forums were held between February 2015 and November 2016, bringing together Texas water experts from business, industry, government, academia, research, and the investment community in impartially facilitated sessions to deter-mine ways to secure Texas’ water future through accelerating growth of infrastructure, technologies, research, education, and sustainable use. Consensus emerged after the first forum that Texas is approaching a water crisis reflecting matters of supply, allocation, and quality that demands immediate action to ensure water security and equitable access to this vital resource. Participant focus rested on new technology acceleration and investment, workforce education, research underway and desired by segments of the water sector, the water-energy-food nexus, outreach and public education, data management and access, water valuation, water security, and legal and regulatory frameworks. Participants also examined funding and partnership options for development of water treatment and supply infrastructure, water rights and allocation methods, aging infrastructure, and conservation, as well as the nearly ubiquitous fragmenting and compartmentalizing of just about everything having to do with water throughout the entire water sector. The forums generated and summarized a wealth of information that can be used by any party to make progress toward the goal of building a Texas water roadmap. This report summarizes the discussions and the path forward for securing Texas’ water resources

Delayed Appearance of High Altitude Retinal Hemorrhages

When closely examined, a very large amount of climbers exhibit retinal hemorrhages during exposure to high altitudes. The incidence of retinal hemorrhages may be greater than previously appreciated as a definite time lag was observed between highest altitude reached and development of retinal bleeding. Retinal hemorrhages should not be considered warning signs of impending severe altitude illness due to their delayed appearance

MHCII glycosylation modulates Bacteroides fragilis carbohydrate antigen presentation

N-linked glycans on class II MHC molecules are required for the presentation of glycoantigens, but not peptide antigens

Human leukocyte antigen (HLA) class II peptide flanking residues tune the immunogenicity of a human tumor-derived epitope

CD4+ T-cells recognize peptide antigens, in the context of human leukocyte antigen (HLA) class II molecules (HLA-II), which through peptide-flanking residues (PFRs) can extend beyond the limits of the HLA binding. The role of the PFRs during antigen recognition is not fully understood; however, recent studies have indicated that these regions can influence T-cell receptor (TCR) affinity and pHLA-II stability. Here, using various biochemical approaches including peptide sensitivity ELISA and ELISpot assays, peptide-binding assays and HLA-II tetramer staining, we focused on CD4+ T-cell responses against a tumor antigen, 5T4 oncofetal trophoblast glycoprotein (5T4), which have been associated with improved control of colorectal cancer. Despite their weak TCR-binding affinity, we found that anti-5T4 CD4+ T-cells are polyfunctional and that their PFRs are essential for TCR recognition of the core bound nonamer. The high-resolution (1.95 Å) crystal structure of HLA-DR1 presenting the immunodominant 20-mer peptide 5T4111–130, combined with molecular dynamic simulations, revealed how PFRs explore the HLA-proximal space to contribute to antigen reactivity. These findings advance our understanding of what constitutes an HLA-II epitope and indicate that PFRs can tune weak affinity TCR–pHLA-II interactions

Modification of the carboxy-terminal flanking region of a universal influenza epitope alters CD4+ T-cell repertoire selection

Human CD4+ αβ T cells are activated via T-cell receptor recognition of peptide epitopes presented by major histocompatibility complex (MHC) class II (MHC-II). The open ends of the MHC-II binding groove allow peptide epitopes to extend beyond a central nonamer core region at both the amino- and carboxy-terminus. We have previously found that these non-bound C-terminal residues can alter T cell activation in an MHC allele-transcending fashion, although the mechanism for this effect remained unclear. Here we show that modification of the C-terminal peptide-flanking region of an influenza hemagglutinin (HA305−320) epitope can alter T-cell receptor binding affinity, T-cell activation and repertoire selection of influenza-specific CD4+ T cells expanded from peripheral blood. These data provide the first demonstration that changes in the C-terminus of the peptide-flanking region can substantially alter T-cell receptor binding affinity, and indicate a mechanism through which peptide flanking residues could influence repertoire selection

Quantification of Optic Disc Edema during Exposure to High Altitude Shows No Correlation to Acute Mountain Sickness

BACKGROUND: The study aimed to quantify changes of the optic nerve head (ONH) during exposure to high altitude and to assess a correlation with acute mountain sickness (AMS). This work is related to the Tuebingen High Altitude Ophthalmology (THAO) study. METHODOLOGY/PRINCIPAL FINDINGS: A confocal scanning laser ophthalmoscope (cSLO, Heidelberg Retina Tomograph, HRT3®) was used to quantify changes at the ONH in 18 healthy participants before, during and after rapid ascent to high altitude (4559 m). Slitlamp biomicroscopy was used for clinical optic disc evaluation; AMS was assessed with Lake Louise (LL) and AMS-cerebral (AMS-c) scores; oxygen saturation (SpO₂) and heart rate (HR) were monitored. These parameters were used to correlate with changes at the ONH. After the first night spent at high altitude, incidence of AMS was 55% and presence of clinical optic disc edema (ODE) 79%. Key stereometric parameters of the HRT3® used to describe ODE (mean retinal nerve fiber layer [RNFL] thickness, RNFL cross sectional area, optic disc rim volume and maximum contour elevation) changed significantly at high altitude compared to baseline (p<0.05) and were consistent with clinically described ODE. All changes were reversible in all participants after descent. There was no significant correlation between parameters of ODE and AMS, SpO₂ or HR. CONCLUSIONS/SIGNIFICANCE: Exposure to high altitude leads to reversible ODE in the majority of healthy subjects. However, these changes did not correlate with AMS or basic physiologic parameters such as SpO₂ and HR. For the first time, a quantitative approach has been used to assess these changes during acute, non-acclimatized high altitude exposure. In conclusion, ODE presents a reaction of the body to high altitude exposure unrelated to AMS

Characterization of Structural Features Controlling the Receptiveness of Empty Class II MHC Molecules

MHC class II molecules (MHC II) play a pivotal role in the cell-surface presentation of antigens for surveillance by T cells. Antigen loading takes place inside the cell in endosomal compartments and loss of the peptide ligand rapidly leads to the formation of a non-receptive state of the MHC molecule. Non-receptiveness hinders the efficient loading of new antigens onto the empty MHC II. However, the mechanisms driving the formation of the peptide inaccessible state are not well understood. Here, a combined approach of experimental site-directed mutagenesis and computational modeling is used to reveal structural features underlying “non-receptiveness.” Molecular dynamics simulations of the human MHC II HLA-DR1 suggest a straightening of the α-helix of the β1 domain during the transition from the open to the non-receptive state. The movement is mostly confined to a hinge region conserved in all known MHC molecules. This shift causes a narrowing of the two helices flanking the binding site and results in a closure, which is further stabilized by the formation of a critical hydrogen bond between residues αQ9 and βN82. Mutagenesis experiments confirmed that replacement of either one of the two residues by alanine renders the protein highly susceptible. Notably, loading enhancement was also observed when the mutated MHC II molecules were expressed on the surface of fibroblast cells. Altogether, structural features underlying the non-receptive state of empty HLA-DR1 identified by theoretical means and experiments revealed highly conserved residues critically involved in the receptiveness of MHC II. The atomic details of rearrangements of the peptide-binding groove upon peptide loss provide insight into structure and dynamics of empty MHC II molecules and may foster rational approaches to interfere with non-receptiveness. Manipulation of peptide loading efficiency for improved peptide vaccination strategies could be one of the applications profiting from the structural knowledge provided by this study