Development of Biomarkers for Endocrine Disrupting Compounds in the Fathead Minnow, \u3cem\u3ePimephales promelas\u3c/em\u3e

Organic waste compounds (OWC’s) are an environmental concern that can have adverse effects on humans, animals, and the environment. Ethinyl estradiol (EE2) is one common OWC and is an active ingredient in oral birth control pills. The presence of EE2 in aquatic environments and drinking water can be linked to decreased fertility and altered physiology in fish. I am developing Q-PCR biomarkers to detect changes in fish at low concentrations before adverse effects occur at higher concentrations. RNA was extracted from twenty fathead minnows that had not been exposed to OWC’s and will be used to synthesize cDNA. Thus far I have tested Q-PCR primers to measure vitellogenin RNA transcription. A standard curve and melt curve demonstrated proper amplification of a single product. A 96 hour exposure of 40 fathead minnows was conducted using EE2 at 0, 3, 17 and 100ng/L. Three 96 hour exposures of triclosan using 36 fathead minnows was conducted at 0, 0.1, 1 ug/L. Livers were dissected from the fish and are currently stored in the -80°C freezer to be used in the future

Bogart Duplo de Bogart: pistas da persona cinematográfica de Humprhrey Bogart, 1941-1946

In the book Bogart duplo de Bogart, Luis Felipe Sobral centralizes his analysisof the construction of the cinematographic persona of Humpfrey Bogart, a Hollywood actor who began his theater career and in supporting characters in the gangster films of the 1930s, but got known for his detective movie main characters in the 1940s.No livro, Bogart duplo de Bogart, Luís Felipe Sobral centraliza sua análise na construção da persona cinematográfica de Humpfrey Bogart, ator hollywoodiano que iniciou sua carreira no teatro e em papéis secundários feitos por ele nos filmes de gângsteres da década de 1930, mas que ficou conhecido por seus papéis de protagonista de filmes de detetive na década de 1940

O. Boulnois – I. Moulin (édité par), Le beau et la beauté au Moyen Âge

xx

Analysis of clinicopathological features of oral squamous cell carcinoma in young patients from Cascavel - Paraná

Orientadores: Marcio Ajudarte Lopes, Ana Lúcia Carrinho Ayroza RangelTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: O carcinoma espinocelular (CEC) é considerado uma doença relativamente incomum em pacientes com idade inferior a 40 anos e existem especulações que este tumor apresenta um comportamento biológico mais agressivo neste grupo. Sendo assim, o objetivo deste estudo foi analisar o perfil clinicopatológico de pacientes jovens (? 40 anos) com CEC oral e correlacioná-lo com o de um grupo controle (? 50 anos) através de quatro sistemas de gradação histopatológica ¿ (1) Sistema da Organização Mundial de Saúde ¿ Sistema OMS, (2) Sistema de Gradação de Malignidade de Margens Invasivas Profundas ¿ Sistema MG, (3) Modelo de Risco Histológico ¿ Sistema HR e (4) Escore de risco BD. Foram selecionados 14 pacientes jovens e 14 pacientes controle com similar estadiamento clínico e localização do tumor. Dados demográficos e clínicos foram obtidos de prontuários de pacientes e os cortes histológicos das peças cirúrgicas emblocadas em parafina foram avaliados de acordo com os quatro sistemas de gradação. As associações entre as categorias foram realizadas através do teste de Qui-quadrado ou teste Exato de Fischer. As análises de sobrevida foram realizadas de acordo com o método de Kaplan-Meier. A comparação entre os grupos mostrou maior associação de modalidades de tratamento em pacientes jovens (p=0.022) e que estes apresentaram maior taxa de recidiva local e metástase regional (p=0.018 / OR= 3.998). Pacientes jovens tiveram menor sobrevida livre de doença em 5 anos (p=0.069). Não houve diferença na sobrevida global em 5 anos entre grupos estudados (p=0.376). Não houve diferença na gradação histológica entre os grupos estudados de acordo com os quatro sistemas utilizados (OMS, MG, HR e BD). Nos sistemas HR e BD mais tumores foram classificados como de alto risco prognóstico que nos sistemas OMS e MG. Este estudo mostrou que, apesar de o grau de diferenciação histológica dos tumores ter sido semelhante entre os grupos e terem sido utilizadas mais modalidades terapêuticas (cirurgia, radioterapia e quimioterapia adjuvantes) no grupo jovem, maior incidência de recidivas e metástases foi observado em pacientes jovens, mostrando uma tendência de um comportamento mais agressivoAbstract: Squamous cell carcinoma (SCC) is considered a relatively uncommon disease in patients younger than 40 years old and there are speculations that this tumor has a more aggressive biological behavior in this group. Thus, the aim of this study was to analyze the clinicopathologic profile of young patients (? 40 years) with oral squamous cell carcinoma and correlate with a control group (? 50 years) by means of four histopathological grading systems - (1) World Health Organization System - WHO System (2) Deep Invasive Margins Deep Malignancy Grading System - MG System, (3) Histologic Risk Model - HR System, and (4) BD Risk Score. Fourteen young patients and 14 control patients with similar clinical stage and tumor location were selected. Demographic and clinical data were obtained from patient's records and histological sections of the paraffin-embedded blocks of surgical specimens were evaluated according to four histopathological grading systems. Associations between categories were performed through Chi-square test and Exact Fisher test. The survival analyzes were performed according to Kaplan-Meier method. The comparison between groups showed that a greater association of treatment modalities in younger patients (p = 0.022) and these had a higher incidence of local recurrence and regional metastasis (p = 0.018 / OR=3.998). Younger patients had lower disease-free survival in 5 years (p = 0.069). There was no difference in overall 5-year survival between the studied groups (p=0.376). There was no difference in histological grading between groups according to the four used systems (WHO, MG, HR and BD). In HR and BD systems more tumors were classified as high risk prognosis than in WHO and MG systems. This study showed that, despite tumors histologic grade was similar between groups and more therapeutic modalities (surgery, adjuvant radiotherapy and chemotherapy) were used in the young group, higher incidence of recurrence and metastasis were observed in young patients, showing a tendency to a more aggressive behaviorDoutoradoPatologiaDoutora em Estomatopatologi

Taxonomic revision of the Neotropical Lauxaniidae genus Neopachycerina Malloch, 1933 (Diptera: Schizophora), with description of a new species

A systematic review of the Neotropical Lauxaniidae genus Neopachycerina Malloch, 1933 is presented. The genus is characterized mainly based on some features of the head, like the anterior pair of fronto-orbital setae conspicuously shorter than posterior one and rather close to them; face with a distinct elevation between antennal bases; antenna slightly elongate, first flagellomere tapered to tip and about three times as long as its basal width, arista thickened by dense black short hairs both above and below, with some longer outstanding hairs on upper side basally. Although some authors have grouped it with some other genera based on chaetotaxy of the head, it was considered closer to Parapachycerina Stuckenberg. Other authors, on the other hand, noted very important differences in the male terminalia, shape of the syntergosternite 7+8 and phallic complex, stressing a view that there are many convergent similarities in unrelated Old and New World lauxaniid genera. This shows that there is some conflicting evidence about the position of Neopachycerina within the Lauxaniidae, but a precise affiliation of Neopachycerina to any genus or group of genera still depends on a global cladistic analysis of the Lauxaniidae genera. In the present paper, Neopachycerina nigra sp. nov., from Argentina, is described and N. aristata Malloch, 1933 is redescribed, with illustrations of both species

Taxonomic revision of the Neotropical Lauxaniidae genus Neopachycerina Malloch, 1933 (Diptera: Schizophora), with description of a new species

A systematic review of the Neotropical Lauxaniidae genus Neopachycerina Malloch, 1933 is presented. The genus is characterized mainly based on some features of the head, like the anterior pair of fronto-orbital setae conspicuously shorter than posterior one and rather close to them; face with a distinct elevation between antennal bases; antenna slightly elongate, first flagellomere tapered to tip and about three times as long as its basal width, arista thickened by dense black short hairs both above and below, with some longer outstanding hairs on upper side basally. Although some authors have grouped it with some other genera based on chaetotaxy of the head, it was considered closer to Parapachycerina Stuckenberg. Other authors, on the other hand, noted very important differences in the male terminalia, shape of the syntergosternite 7+8 and phallic complex, stressing a view that there are many convergent similarities in unrelated Old and New World lauxaniid genera. This shows that there is some conflicting evidence about the position of Neopachycerina within the Lauxaniidae, but a precise affiliation of Neopachycerina to any genus or group of genera still depends on a global cladistic analysis of the Lauxaniidae genera. In the present paper, Neopachycerina nigra sp. nov., from Argentina, is described and N. aristata Malloch, 1933 is redescribed, with illustrations of both species

Comparative study between the effects of isolated manual therapy techniques and those associated with low level laser therapy on pain in patients with temporomandibular dysfunction

Objective: This study sought to evaluate the pain condition in patients with temporomandibular dysfunction after applying manual therapy techniques and those associated with this low level laser therapy. Methods: The study involved 20 patients with temporomandibular dysfunction, divided randomly into two groups: G1 (n = 10), formed by 7 women and 3 men, average age 28.2 years (± 7), treated with manual therapy techniques and G2 (n = 10), formed by 8 women and 2 men, with average age 24.01 (± 6.04), treated with the combination of manual therapy techniques and low level laser therapy. The patients were treated three times a week for four consecutive weeks. The memorandum of manual therapy techniques based on Chaintow,Makofsky and Bienfaint was used. For low level laser therapy GaAs laser (904 nm), 6 J/cm2, 0.38 mW/cm2 was used, applied at 4pre-auricular points. To analyze the pain level, the visual analog pain scale was used. For data analysis the Student’s-t and Wilcoxon tests were used, both with significance level of 5% (p <0.05).Results: There was significant reduction (p <0.05) in the level of pain in both groups treated, but in G2 the significance was higher.Conclusion: Manual therapy techniques, either alone or associated with low level laser therapy showed satisfactory results for pain control in patients with temporomandibular dysfunction

Ciclovias: A utilização da bicicleta como forma de desenvolvimento sustentável, estudo AV. Brasil, Cascavel - Paraná - Brasil

O objetivo deste trabalho foi analisar se houve aumento no número de ciclistas que trafegam na ciclovia ao longo da Av. Brasil após a melhoria da mesma com a implantação do Programa de Desenvolvimento Integrado – PDI/BID. A metodologia utilizada foi a contagem do número de ciclistas em três locais: Avenida Brasil/Barão do Rio Branco, Av. Brasil/Rua Sete de Setembro e Av. Brasil/Rua Manoel Ribas (Praça do Migrante). A pesquisa demonstrou que a média de ciclistas que passaram a trafegar na ciclovia da Av. Brasil, após sua implantação do Programa de Desenvolvimento Integrado, foi de 683 ciclistas/dia, isto é, um aumento de 68,23% em relação à situação anterior. O tema analisado neste trabalho serve como sugestão ao poder público a implantação de novos trechos de ciclovias nos principais eixos da cidade, que contribuirá para o desenvolvimento da cidade e melhora da qualidade de vida da população, diretamente em aspectos ambientais, sociais e econômicos

Mechanisms regulating the circannual rhythm of hibernation

Thesis (Ph.D.) University of Alaska Fairbanks, 2019Hibernation is a unique adaptation to conserve energy entering a hypometabolic (low metabolic rate) and hypothermic (low body temperature) state called torpor. Torpor is characterized by a drop in metabolism to 1-2% of basal metabolic rate and a decrease in body temperature to one to two degrees above ambient temperature. Metabolic rate is restored to basal metabolic rate and body temperature increases from 2-3⁰C to 36⁰C during the regularly timed arousal. The adenosine A1 receptor agonists promote the onset of hibernation and torpor in different species, through a yet undefined neuronal circuit. In the Arctic ground squirrel, CHA, an adenosine A1 receptor agonist, induces hibernation during the winter- hibernation season but not in summer even when the environmental conditions are kept constant (ambient temperature of 2⁰C and a light cycle of 4L:20D). Thus, the phenomenon of CHA-induced hibernation is entrained to an endogenous circannual rhythm. In this work, I aim to identify the changes in neuronal activation that reflect the circannual rhythm regulating the seasonal difference in response to CHA. Arctic ground squirrels, housed at constant ambient temperature (2°C) and light cycle (4L:20D), were implanted with body temperature transmitters. I collected tissue during Summer, Fall, Winter and Torpor conditions for seasonal analysis. For treatment analysis, I collected tissue form animals treated with CHA or vehicle in Summer and Winter. Primarily, I used immunohistochemistry to identify cell groups affected by season and treatment. I used cFos to identify neuronal activity and other immunohistochemical markers to identify neuronal phenotypes, based on specific cytoplasmic proteins. An overall seasonal decrease in thermogenesis, measured as reduced neuronal activity in the thermoregulatory pathways, and increase in vasoconstriction reflected the higher order processing necessary for CHA-induced hibernation. CHA inhibited the histaminergic neurons in the hypothalamus suppressing wakefulness and dis-inhibited the nucleus tractus solitarius, further suppressing thermogenesis. Preliminary data also suggested a seasonal change in the adenosine metabolic pathway, which may have increased adenosine receptor sensitivity during the hibernation season. Our results suggest that histaminergic neurons in the hypothalamus and the nucleus tractus solitarius are likely targets to manipulate metabolic demand in the clinical setting inducing therapeutic hypothermia or increasing metabolic rate.Institute of Arctic BiologyChapter 1: General introduction -- Chapter 2: The raphe pallidus and the hypothalamic-pituitary-thyroid axis gate seasonal changes in thermoregulation in the hibernating Arctic Ground Squirrel (Urocitellus Parryii) -- Chapter 3: Seasonal decrease in thermogenesis and increase in vasoconstriction explain seasonal response to N⁶-cyclohexyladenosine -induced hibernation in the Arctic Ground Squirrel (Urocitellus parryii) -- Chapter 4: Seasonal changes in adenosine kinase in tanycytes underlie the possible mechanism of adenosine-induced hibernation in the Arctic Ground Squirrel (Urocitellus parryii) -- Chapter 5: General conclusions -- Appendices

A atuação do psicólogo na prática de assistência social na região da Foz de Rio Itajaí do estado de Santa Catarina

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Programa de Pós-Graduação em Psicologia, Florianópolis, 20132013-12-05T22:38:50