Consumption patterns: A proposed model for measurement of solution palatability in pigs

In animal production, the palatability of feeds or solutions has typically been inferred from measurements of preference or acceptance. However, laboratory studies in rats have demonstrated that palatability quantified through the analysis of the microstructure of licking can dissociate from simple measures of consumption. The aim of this study was to evaluate palatability in pigs by using consumption patterns. Pigs (n = 24) were exposed (in pairs, with video recording) to different sucrose solutions (0.5, 1, 2, 4, 8, 16, and 32%) over 7 consecutive 10-min tests (1 concentration/d). Total consumption, number of consumption approaches (A), and real consumption time (RCT) were measured. Palatability was estimated through consumption pattern (RCT/A), analogous to the licks/bout measure used in rats. Data was analyzed by sucrose concentration. Spearman correlation coefficients were estimated between the logarithm of sucrose concentration and total consumption, A, RCT, and RCT/A. Total consumption and RCT showed inverted U functions relative to sucrose concentration. Consumption pattern (RCT/A) presented a dose effect (P < 0.005) and positive correlations with sucrose concentration (R = 0.23, P = 0.034). As with rats, consumption pattern could represent an interesting and novel measure of feeding behavior, reflecting palatability in pigs

Improving broiler performance at market age regardless of stocking density by using a pre-starter diet

Altres ajuts: Centro para el Desarrollo Tecnológico Industrial CDTI project IDI-20180885Broiler early nutrition has caught the attention of nutritionists due to the positive correlation between early growth rate and market weight. Early nutrition strategies such as low Ca levels or the use of highly digestible ingredients (e.g., spray-dried porcine plasma [SDPP]) have been reported to improve gut and muscle development, immunity, and overall growth of the bird. On the other hand, recent works suggested that stocking density represents the main constrain for modern chickens to express their full genetic potential. The current study aimed to elucidate the potential effects of pre-starter feeding strategies on contrasting livestock stocking densities. The study followed a factorial design of 2 contrasting starter programs (standard [0-11d] vs. pre-starter [0-4 d] + standard [4-11 d]) by 3 stocking densities (low, medium, and high, 27, 33, and 39 kg/BW/m, respectively). Birds placed at low stocking density showed higher BW and better FCR than those at high stocking density (P < 0.05) being this evident only after d 28. On the other hand, birds fed on pre-starter diet led to higher BW at 4 d (P < 0.001) and the effects on BW were maintained until market age (+2.54%; P < 0.05). The pre-starter diet also led to heavier carcasses (+2.2%; P < 0.05), improved uniformity (P < 0.10) and showed a lower incidence of lameness at high stocking densities (P < 0.05) compared to birds fed the standard starter diet. Results of the current study confirm that, regardless of the stocking density used, pre-starter diets (0-4 d) could lead to a better early growth and market weight

High metabolomic microdiversity within co-occurring isolates of the extremely halophilic bacterium Salinibacter ruber

Salinibacter ruber is an extremely halophilic member of the Bacteroidetes that thrives in crystallizer ponds worldwide. Here, we have analyzed two sets of 22 and 35 co-occurring S. ruber strains, newly isolated respectively, from 100 microliters water samples from crystalizer ponds in Santa Pola and Mallorca, located in coastal and inland Mediterranean Spain and 350 km apart from each other. A set of old strains isolated from the same setting were included in the analysis. Genomic and taxonomy relatedness of the strains were analyzed by means of PFGE and MALDI-TOF, respectively, while their metabolomic potential was explored with high resolution ion cyclotron resonance Fourier transform mass spectrometry (ICR-FT/MS). Overall our results show a phylogenetically very homogeneous species expressing a very diverse metabolomic pool. The combination of MALDI-TOF and PFGE provides, for the newly isolated strains, the same scenario presented by the previous studies of intra-specific diversity of S. ruber using a more restricted number of strains: the species seems to be very homogeneous at the ribosomal level while the genomic diversity encountered was rather high since no identical genome patterns could be retrieved from each of the samples. The high analytical mass resolution of ICR-FT/MS enabled the description of thousands of putative metabolites from which to date only few can be annotated in databases. Some metabolomic differences, mainly related to lipid metabolism and antibiotic-related compounds, provided enough specificity to delineate different clusters within the co-occurring strains. In addition, metabolomic differences were found between old and new strains isolated from the same ponds that could be related to extended exposure to laboratory conditions.This work was supported by the projects CLG2009-12651-C02-01 and 02; and CE-CSD2007-0005 of the Spanish Ministry of Science and Innovation, and all three projects were also co-financed with FEDER support from the European Union. JBE was financed by the Government of the Balearic Islands, Ministry of Economy and Finances

ESICM LIVES 2016: part two : Milan, Italy. 1-5 October 2016.

Meeting abstrac