Experimental Verification of Rate Flexibility and Probabilistic Shaping by 4D Signaling

The rate flexibility and probabilistic shaping gain of $4$-dimensional signaling is experimentally tested for short-reach, unrepeated transmission. A rate granularity of 0.5 bits/QAM symbol is achieved with a distribution matcher based on a simple look-up table.Comment: Presented at OFC'18, San Diego, CA, US

Experimental Comparison of Probabilistic Shaping Methods for Unrepeated Fiber Transmission

\u3cp\u3eThis paper studies the impact of probabilistic shaping on effective signal-to-noise ratios (SNRs) and achievable information rates (AIRs) in a back-to-back configuration and in unrepeated nonlinear fiber transmissions. For the back-to-back setup, various shaped quadrature amplitude modulation (QAM) distributions are found to have the same implementation penalty as uniform input. By demonstrating in transmission experiments that shaped QAM input leads to lower effective SNR than uniform input at a fixed average launch power, we experimentally confirm that shaping enhances the fiber nonlinearities. However, shaping is ultimately found to increase the AIR, which is the most relevant figure of merit, as it is directly related to spectral efficiency. In a detailed study of these shaping gains for the nonlinear fiber channel, four strategies for optimizing QAM input distributions are evaluated and experimentally compared in wavelength division multiplexing (WDM) systems. The first shaping scheme generates a Maxwell-Boltzmann (MB) distribution based on a linear additive white Gaussian noise channel. The second strategy uses the Blahut-Arimoto algorithm to optimize an unconstrained QAM distribution for a split-step Fourier method based channel model. In the third and fourth approach, MB-shaped QAM and unconstrained QAM are optimized via the enhanced Gaussian noise (EGN) model. Although the absolute shaping gains are found to be relatively small, the relative improvements by EGN-optimized unconstrained distributions over linear AWGN optimized MB distributions are up to 59%. This general behavior is observed in 9-channel and fully loaded WDM experiments.\u3c/p\u3

Kiri N. Chr. L. Abrahams'ile

Forchhammer Johann Georg, 1794-1865, taani mineraloogAbrahams, Nicolai Christian Levin, 1798-?, taani arheoloog ja kirjandusteadlan

Novel Insights into the Regulation of LexA in the Cyanobacterium Synechocystis sp. Strain PCC 6803 ▿ †

The transcription factor LexA in the cyanobacterium Synechocystis sp. strain PCC 6803 has been shown to regulate genes that are not directly involved in DNA repair but instead in several different metabolic pathways. However, the signal transduction pathways remain largely uncharacterized. The present work gives novel insights into the regulation of LexA in this unicellular cyanobacterium. A combination of Northern and Western blotting, using specific antibodies against the cyanobacterial LexA, was employed to show that this transcription regulator is under posttranscriptional control, in addition to the classical and already-described transcriptional regulation. Moreover, detailed two-dimensional (2D) electrophoresis analyses of the protein revealed that LexA undergoes posttranslational modifications. Finally, a fully segregated LexA::GFP (green fluorescent protein) fusion-modified strain was produced to image LexA's spatial distribution in live cells. The fusion protein retains DNA binding capabilities, and the GFP fluorescence indicates that LexA is localized in the innermost region of the cytoplasm, decorating the DNA in an evenly distributed pattern. The implications of these findings for the overall role of LexA in Synechocystis sp. strain PCC 6803 are further discussed

A new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer

Background: We previously showed that the bacterial lipopeptide Pam3Cys-Ser-Ser, meanwhile established as a toll-like receptor (TLR) 1/2 ligand, acts as a strong adjuvant for the induction of virus specific CD8+ T cells in mice, when covalently coupled to a synthetic peptide.Case presentation: We now designed a new water-soluble synthetic Pam3Cys-derivative, named XS15 and characterized it in vitro by a TLR2 NF-κB luciferase reporter assay. Further, the capacity of XS15 to activate immune cells and stimulate peptide-specific CD8+ T and NK cells by 6-sulfo LacNAc+ monocytes was assessed by flow cytometry as well as cytokine induction using immunoassays. The induction of a functional immune response after vaccination of a volunteer with viral peptides was assessed by ELISpot assay and flow cytometry in peripheral blood cells and infiltrating cells at the vaccination site, as well as by immunohistochemistry and imaging.XS15 induced strong ex vivo CD8+ and TH1 CD4+ responses in a human volunteer upon a single injection of XS15 mixed to uncoupled peptides in a water-in-oil emulsion (Montanide™ ISA51 VG). A granuloma formed locally at the injection site containing highly activated functional CD4+ and CD8+ effector memory T cells. The total number of vaccine peptide-specific functional T cells was experimentally assessed and estimated to be 3.0 × 105 in the granuloma and 20.5 × 106 in peripheral blood.Conclusion: Thus, in one volunteer we show a granuloma forming by peptides combined with an efficient adjuvant in a water-in-oil-emulsion, inducing antigen specific T cells detectable in circulation and at the vaccination site, after one single vaccination only. The ex vivo T cell responses in peripheral blood were detectable for more than one year and could be strongly boosted by a second vaccination. Hence, XS15 is a promising adjuvant candidate for peptide vaccination, in particular for tumor peptide vaccines in a personalized setting