Influence of cell surface and nanomechanical properties on the flocculation ability of industrial <i>Saccharomyces cerevisiae</i> strains

In the past few years, atomic force microscopy (AFM) has provided novel information on the ultrastructural and nanomechanical properties of yeast cell walls that play a major role in determining the flocculation characteristics of the yeasts. In this study, we used AFM to visualize at the nanoscale the cell surface topography and to determine cell wall nanomechanical properties (e.g. elasticity) of different strains of S. cerevisiae employed for brewing, winemaking and fuel alcohol production. Cell surface topography was found to correlate with the flocculation behaviour of these strains during their late stationary phase, with the cell surface of flocculent cells being rougher than that of weakly flocculent cells. The elastic modulus of the yeast cell walls showed that weakly flocculent strains had a more rigid cell wall than highly flocculent strains. This difference in elasticity seemed to have an effect on the adhesive properties of the yeast cell walls, with weakly flocculent yeasts displaying higher adhesion energy than the highly flocculent strains. These findings seem to indicate that yeast cell surface nanomechanical properties play an important role in governing flocculation

W252 Land-filling Large Animal Mortalities in Tennessee

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W255 Disposing of Large Animal Mortalities in Tennessee

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Flocculation in industrial strains of <i>Saccharomyces cerevisiae</i>:role of cell wall polysaccharides and lectin-like receptors

Yeast flocculation is the reversible aggregation of yeast cells promoted by the interaction between lectin-like protein receptors with mannose side chains on adjacent cell walls. Flocculation is governed by several physiological factors, including the type of nutrient sugar available to yeast. We grew four industrial strains of Saccharomyces cerevisiae, representing applications in the brewing, winemaking and bioethanol sectors, to late stationary phase and quantified the cellular content of mannans, glucans and lectin-like proteins on yeast cell surfaces. Results indicated that brewing and champagne strains showed moderate to high flocculation ability when grown with glucose, fructose, maltose or galactose, whereas winemaking and fuel alcohol strains only showed moderate flocculation when grown on maltose and galactose. All yeast strains studied were weakly flocculent when grown on mannose. With regard to lectin-like receptors, their number played a more important role in governing yeast flocculation than the mannan and glucan contents in yeast cell walls. We conclude that all the industrial strains of S. cerevisiae belonged to New-Flo type on the basis of their flocculation behaviour observed when cultured on different sugars. Quantification of yeast cell wall polysaccharides and receptor sites indicates that mannan and glucan levels remain almost constant, irrespective of the strain under investigation. The main difference in flocculation characteristics in industrial yeast strains appears to be due to variations in concentrations of lectin-like cell surface receptors. Our findings may benefit brewers, winemakers and other yeast-based technologies in design of media to prevent premature flocculation during fermentation

Eurasian Arctic greening reveals teleconnections and the potential for novel ecosystems

Arctic warming has been linked to observed increases in tundra shrub cover and growth in recent decades on the basis of significant relationships between deciduous shrub growth/biomass and temperature. These vegetation trends have been linked to Arctic sea ice decline and thus to the sea ice/albedo feedback known as Arctic amplification. However, the interactions between climate, sea ice and tundra vegetation remain poorly understood. Here we reveal a 50- year growth response over a >100,000 km2 area to a rise in summer temperature for alder (Alnus) and willow (Salix), the most abundant shrub genera respectively at and north of the continental treeline. We demonstrate that whereas plant productivity is related to sea ice in late spring, the growing season peak responds to persistent synoptic-scale air masses over West Siberia associated with Fennoscandian weather systems through the Rossby wave train. Substrate is important for biomass accumulation, yet a strong correlation between growth and temperature encompasses all observed soil types. Vegetation is especially responsive to temperature in early summer. These results have significant implications for modelling present and future Low Arctic vegetation responses to climate change, and emphasize the potential for structurally novel ecosystems to emerge fromwithin the tundra zone.Vertaisarviointia edeltävä käsikirjoitu

Variational semi-blind sparse deconvolution with orthogonal kernel bases and its application to MRFM

We present a variational Bayesian method of joint image reconstruction and point spread function (PSF) estimation when the PSF of the imaging device is only partially known. To solve this semi-blind deconvolution problem, prior distributions are specified for the PSF and the 3D image. Joint image reconstruction and PSF estimation is then performed within a Bayesian framework, using a variational algorithm to estimate the posterior distribution. The image prior distribution imposes an explicit atomic measure that corresponds to image sparsity. Importantly, the proposed Bayesian deconvolution algorithm does not require hand tuning. Simulation results clearly demonstrate that the semi-blind deconvolution algorithm compares favorably with previous Markov chain Monte Carlo (MCMC) version of myopic sparse reconstruction. It significantly outperforms mismatched non-blind algorithms that rely on the assumption of the perfect knowledge of the PSF. The algorithm is illustrated on real data from magnetic resonance force microscopy (MRFM)

On the structure of globular cluster systems in elliptical galaxies

It has long been known that the radial density profiles of globular cluster systems (GCSs) in elliptical galaxies vary with the total luminosities of their host galaxies. In order to elucidate the origin of this structural non-homology in GCSs, we numerically investigate the structural properties of GCSs in elliptical galaxies formed from a sequence of major dissipationless galaxy merging. We find that the radial density profiles of GCSs in elliptical galaxies become progressively flatter as the galaxies experience more major merger events. The density profiles of GCSs in ellipticals are well described as power-laws with slopes (${\alpha}_{\rm gc}$) ranging from -2.0 to -1.0. They are flatter than, and linearly proportional to, the slopes (${\alpha}_{\rm s}$) of the stellar density profiles of their host galaxies. We also find that the GCS core radii ($r_{\rm c}$) of the density profiles are larger in ellipticals that experienced more mergers. By applying a reasonable scaling relation between luminosities and sizes of galaxies to the simulation results, we show that ${\alpha}_{\rm gc} \approx -0.36 M_{\rm V}-9.2$, $r_{\rm c} \approx -1.85 M_{\rm V}$, and ${\alpha}_{\rm gc} \approx 0.93 {\alpha}_{\rm s}$, where $M_{\rm V}$ is the total $V$-band absolute magnitude of a galaxy.Comment: 8 pages, 8 figures, accepted by A&

Cassava (Manihot esculenta Crantz) Tuber Quality as Measured by Starch and Cyanide (HCN) Affected by Nitrogen, Phosphorus, and Potassium Fertilizer Rates

Cassava (Manihot esculenta Crantz) is an important subsistence crop for many poor rural families in Africa. Cassava contains cyanogenic glucosides (linamarin and lotaustralin) which liberate hydrogen cyanide (HCN) during tuber processing. Once liberated, HCN attaches to the processed tuber. Continuous consumption of processed tuber containing high HCN concentration coupled with low protein intake causes Konzo – a paralyzing disorder that impacts children and women of childbearing age. There are ways to reduce HCN concentration during tuber processing; however, this can also reduce the overall starch content in the cassava tuber. A study comprising twenty treatments consisting of different combinations of nitrogen (N), phosphorus (P), and potassium (K) fertilizer rates was initiated in 2013 in the coastal Dondo District of Mozambique to assess cassava tuber quality as measured by starch and HCN. Significant differences were observed in starch content (CSC) of unprocessed tubers due to combined addition of N, P and K fertilizer rates, sample size, and estimation procedure. However, no significant differences were observed in HCN concentration in tubers due to the addition of N, P and K fertilizer. The HCN concentration in cassava tuber appears to be a function of the physiology of the crop or possibly cassava variety rather than the environment or conditions under which the crop is grown

Effectiveness of herpes zoster vaccination in an older United Kingdom population.

BACKGROUND: Vaccination against herpes zoster was introduced in the United Kingdom in 2013 for individuals aged 70 years, with a phased catch-up campaign for 71-79 year olds. Vaccine introduction has resulted in a marked fall in incident herpes zoster and in post-herpetic neuralgia (PHN), but formal evaluation of vaccine effectiveness is needed. METHODS: In a population-based cohort study of older individuals born between 1933 and 1946, we used linked UK anonymised primary care health records for the first three years of the vaccination programme (01/09/2013-31/08/2016) and multivariable Poisson regression to obtain incidence rates and vaccine effectiveness (VE) against zoster and PHN. RESULTS: Among 516,547 individuals, 21% were vaccinated. Incidence of zoster was 3.15/1000 person-years in vaccinees and 8.80/1000 person-years in unvaccinated individuals. After adjustment, VE was 64% (95%CI = 60-68%) against incident zoster and 81% (95%CI = 61-91%) against PHN, with very similar VE estimates in the routine and catch-up cohorts. VE against zoster was lower in those with a previous history of zoster: 47% (95%CI = 31-58%) versus 64% (95%CI = 60-68%) in those without previous zoster. There was evidence of waning VE over time, from 69% (95%CI = 65-74%) in the first year after vaccination to 45% (95%CI = 29-57%) by the third year. CONCLUSION: This first formal assessment of VE in the UK zoster vaccination programme demonstrates good effectiveness of zoster vaccine, and very good protection against PHN. The findings provide evidence that VE is similar across the age groups targeted for vaccination in the UK, and on duration of protection of the vaccine in public health use. The study provides key information for decision-makers about the future direction of UK zoster vaccination programme, indicating that the live zoster vaccine may be more cost-effective than estimated previously. It also supports efforts to communicate the benefits of zoster vaccination to address the declining coverage observed across the UK