Uterus torsion in Nellore cow : case report

A torção de útero em bovinos é um caso de emergência obstétrica que, na maioria das vezes, leva à morte do feto e da mãe. O tratamento clínico é pouco eficaz e em grande parte dos casos a operação cesariana é indicada. O trabalho descreve o caso de uma vaca Nelore de 36 meses encaminhada ao Hospital Escola de Grandes Animais da Universidade de Brasília com torção uterina e sinais de toxemia. Após tentativas improdutivas de rolamento, o animal foi submetido à cesariana e veio a morrer durante o procedimento cirúrgico. Vários casos de torção de útero em bovinos são descritos em raças europeias, porém, pouco se conhece desse tipo de patologia em animais da raça Nelore. _______________________________________________________________________________ ABSTRACTUterus torsion in cows is an emergency case that usually leads to foetus and cow death. Conservative management has low efficacy and cesarian surgery is indicated in the majority of the cases. The report describes the case of a 36-year-old Nellore cow taken to Hospital Escola de Grandes Animais of Universidade de Brasíla with uterus torsion and toxemia signs. After improductive rolling procedures, the animal was lead to a cesarian surgery and died during the procedure. Uterus torsion is described in european breeds, but this patology is not well-known in Nellore cattle

Presença de Mulheres no Conselho de Administração e Retorno de Ações

O presente trabalho teve como objetivo analisar se a presença de mulheres no Conselho de Administração (C.A.) das empresas impacta o retorno das ações, utilizando um estudo de eventos. Os dados sobre a composição do C.A. foram coletados no site da Comissão de Valores Mobiliários (CVM), e os dados financeiros foram coletados no Banco de Dados Economática. Analisando os eventos em conjunto, o presente estudo não identificou efeito significativo da divulgação no retorno das ações (na análise individualizada por ações, foi observado efeito estatisticamente significante somente em alguns casos na janela de eventos). O trabalho é relevante ao contribuir para a literatura de diversidade de gênero na área de Finanças, especificamente no que tange a estudos abordando a presença de mulheres no C.A. das empresas. A pesquisa também evidencia o aspecto semiforte do mercado de ações brasileiro, considerando a teoria sobre eficiência de mercado

Effects on volume load and ratings of perceived exertion in individuals advanced weight-training after transcranial direct current stimulation

Lattari, E, Rosa Filho, BJ, Fonseca Junior, SJ, Murillo-Rodriguez, E, Rocha, N, Machado, S, and Maranhão Neto, GA. Effects on volume load and ratings of perceived exertion in individuals' advanced weight training after transcranial direct current stimulation. J Strength Cond Res 34(1): 89–96, 2020—The aim of this study was investigate the effects of transcranial direct current stimulation (tDCS) on volume load and ratings of perceived exertion. Fifteen young healthy individuals, aged between 20 and 30 years in advanced strength training were recruited. Test and retest of the 10 maximum repetitions (10RM) were performed to determine the reliability of load used. Subjects performed 3 experimental conditions in a randomized, double-blinded crossover design: anodic stimulation (a-tDCS), cathodic stimulation (c-tDCS), and sham (2 mA for 20 minutes targeting the dorsolateral prefrontal cortex left). Immediately after the experimental conditions, subjects completed 1 set of maximum repetitions with 10RM load (volume load) and answered to OMNI-RES (poststimulation) (level of significance p ≤ 0.05). The volume load showed main effect for condition (F(2, 28) = 164.801; p < 0.001). In poststimulation, a-tDCS was greater than c-tDCS (p ≤ 0.001) and sham (p ≤ 0.001). For ratings of perceived exertion (OMNI-RES), the results showed main effect for condition (F(2, 28) = 9.768; p ≤ 0.05). In poststimulation, c-tDCS was greater than a-tDCS (p ≤ 0.05) and sham (p ≤ 0.05). We conclude that the use of a-tDCS may promote increase in volume load for the LP45 exercise. Moreover, higher volume loads are necessary to maximize muscle strength and anabolism.info:eu-repo/semantics/publishedVersio

Soil organic matter fractions, chemical attributes and aggregation under forestry and agricultural systems

The aim of this work was to evaluate the influence of the conversion of forest systems to agricultural systems in the organic matter compartments, aggregation and soil chemical attributes, in the Atlantic Forest. The evaluated systems were: annual crop (ACr); perennial agriculture (PAg); pasture; and secondary forest early (SFES), medium (SFMS), and advanced stage (SFAS). Soil samples were collected at the layer of 0-5 cm depth and quantified the total organic carbon (TOC), C of humic substances, oxidizable C, granulometric fractions of soil organic matter (SOM), soil chemical attributes, soil aggregation and glomalin-related soil protein (GRSP-total and GRSP-easily extractable) in different aggregate classes. It was observed a reduction of the TOC, particulate organic carbon (POC), humic substances and oxidizable C in the PAg and ACr areas comparing to pasture and forest systems. Moreover, the pH values increased whereas P content decreased in comparison with SFAS. As for aggregation, the PAg and the ACr decreased by around 35% and 20% the mean weight diameter of aggregates, respectively, compared to the average values found in the forestry systems, and 34% and 45%, respectively in relation to pasture. In general, GRSP-total were reduced by agriculture. Thus, it appears that the agriculture which has been practiced is altering negatively the soil chemical, physical and biological attributes.The aim of this work was to evaluate the influence of the conversion of forest systems to agricultural systems in the organic matter compartments, aggregation and soil chemical attributes, in the Atlantic Forest. The evaluated systems were: annual crop (ACr); perennial agriculture (PAg); pasture; and secondary forest early (SFES), medium (SFMS), and advanced stage (SFAS). Soil samples were collected at the layer of 0-5 cm depth and quantified the total organic carbon (TOC), C of humic substances, oxidizable C, granulometric fractions of soil organic matter (SOM), soil chemical attributes, soil aggregation and glomalin-related soil protein (GRSP-total and GRSP-easily extractable) in different aggregate classes. It was observed a reduction of the TOC, particulate organic carbon (POC), humic substances and oxidizable C in the PAg and ACr areas comparing to pasture and forest systems. Moreover, the pH values increased whereas P content decreased in comparison with SFAS. As for aggregation, the PAg and the ACr decreased by around 35% and 20% the mean weight diameter of aggregates, respectively, compared to the average values found in the forestry systems, and 34% and 45%, respectively in relation to pasture. In general, GRSP-total were reduced by agriculture. Thus, it appears that the agriculture which has been practiced is altering negatively the soil chemical, physical and biological attributes

Long noncoding intronic RNAs are differentially expressed in primary and metastatic pancreatic cancer

<p>Abstract</p> <p>Background</p> <p>Pancreatic ductal adenocarcinoma (PDAC) is known by its aggressiveness and lack of effective therapeutic options. Thus, improvement in current knowledge of molecular changes associated with pancreatic cancer is urgently needed to explore novel venues of diagnostics and treatment of this dismal disease. While there is mounting evidence that long noncoding RNAs (lncRNAs) transcribed from intronic and intergenic regions of the human genome may play different roles in the regulation of gene expression in normal and cancer cells, their expression pattern and biological relevance in pancreatic cancer is currently unknown. In the present work we investigated the relative abundance of a collection of lncRNAs in patients' pancreatic tissue samples aiming at identifying gene expression profiles correlated to pancreatic cancer and metastasis.</p> <p>Methods</p> <p>Custom 3,355-element spotted cDNA microarray interrogating protein-coding genes and putative lncRNA were used to obtain expression profiles from 38 clinical samples of tumor and non-tumor pancreatic tissues. Bioinformatics analyses were performed to characterize structure and conservation of lncRNAs expressed in pancreatic tissues, as well as to identify expression signatures correlated to tissue histology. Strand-specific reverse transcription followed by PCR and qRT-PCR were employed to determine strandedness of lncRNAs and to validate microarray results, respectively.</p> <p>Results</p> <p>We show that subsets of intronic/intergenic lncRNAs are expressed across tumor and non-tumor pancreatic tissue samples. Enrichment of promoter-associated chromatin marks and over-representation of conserved DNA elements and stable secondary structure predictions suggest that these transcripts are generated from independent transcriptional units and that at least a fraction is under evolutionary selection, and thus potentially functional.</p> <p>Statistically significant expression signatures comprising protein-coding mRNAs and lncRNAs that correlate to PDAC or to pancreatic cancer metastasis were identified. Interestingly, <it>loci </it>harboring intronic lncRNAs differentially expressed in PDAC metastases were enriched in genes associated to the MAPK pathway. Orientation-specific RT-PCR documented that intronic transcripts are expressed in sense, antisense or both orientations relative to protein-coding mRNAs. Differential expression of a subset of intronic lncRNAs (<it>PPP3CB</it>, <it>MAP3K14 </it>and <it>DAPK1 loci</it>) in metastatic samples was confirmed by Real-Time PCR.</p> <p>Conclusion</p> <p>Our findings reveal sets of intronic lncRNAs expressed in pancreatic tissues whose abundance is correlated to PDAC or metastasis, thus pointing to the potential relevance of this class of transcripts in biological processes related to malignant transformation and metastasis in pancreatic cancer.</p

No-match ORESTES explored as tumor markers

Sequencing technologies and new bioinformatics tools have led to the complete sequencing of various genomes. However, information regarding the human transcriptome and its annotation is yet to be completed. The Human Cancer Genome Project, using ORESTES (open reading frame EST sequences) methodology, contributed to this objective by generating data from about 1.2 million expressed sequence tags. Approximately 30% of these sequences did not align to ESTs in the public databases and were considered no-match ORESTES. On the basis that a set of these ESTs could represent new transcripts, we constructed a cDNA microarray. This platform was used to hybridize against 12 different normal or tumor tissues. We identified 3421 transcribed regions not associated with annotated transcripts, representing 83.3% of the platform. The total number of differentially expressed sequences was 1007. Also, 28% of analyzed sequences could represent noncoding RNAs. Our data reinforces the knowledge of the human genome being pervasively transcribed, and point out molecular marker candidates for different cancers. To reinforce our data, we confirmed, by real-time PCR, the differential expression of three out of eight potentially tumor markers in prostate tissues. Lists of 1007 differentially expressed sequences, and the 291 potentially noncoding tumor markers were provided

No-match ORESTES explored as tumor markers

Sequencing technologies and new bioinformatics tools have led to the complete sequencing of various genomes. However, information regarding the human transcriptome and its annotation is yet to be completed. The Human Cancer Genome Project, using ORESTES (open reading frame EST sequences) methodology, contributed to this objective by generating data from about 1.2 million expressed sequence tags. Approximately 30% of these sequences did not align to ESTs in the public databases and were considered no-match ORESTES. On the basis that a set of these ESTs could represent new transcripts, we constructed a cDNA microarray. This platform was used to hybridize against 12 different normal or tumor tissues. We identified 3421 transcribed regions not associated with annotated transcripts, representing 83.3% of the platform. The total number of differentially expressed sequences was 1007. Also, 28% of analyzed sequences could represent noncoding RNAs. Our data reinforces the knowledge of the human genome being pervasively transcribed, and point out molecular marker candidates for different cancers. To reinforce our data, we confirmed, by real-time PCR, the differential expression of three out of eight potentially tumor markers in prostate tissues. Lists of 1007 differentially expressed sequences, and the 291 potentially noncoding tumor markers were provided

A utilização do Piezoeletricidade na odontologia: Revisão de literatura

Introduction: Piezoelectricity appeared in the middle of the 19th century, the ultrasonic piezo consists of the use of an ultrasonic device, capable of cutting mineralized bone tissue, without damaging the soft tissue, which is one of its main advantages when compared to conventional techniques with drills, saws and instrumental. Objective: This study aims to conduct a literature review about the various uses of piezoelectricity in dentistry. Materials and Methods: For the preparation of this article, a narrative review of the literature was carried out, using the databases: PUBMED / MEDLINE, Google academic, Scielo, Revodonto, LILACS, BBO and CAPES Portal. Articles were selected that addressed the subjects of ultrasound surgery, piezosurgery, alternative surgical techniques and surgical vibration devices. Discussion: According to the theory of the Curie brothers, the piezoelectric effect occurs through the use of specific types of ceramics and crystals such as quartz, which undergo deformations when an electric current pass through them, resulting in ultrasonic frequency oscillations and when applied. if on a surface these mechanical vibrations generate a rupture in the molecular cohesion cause cavitations on this surface. Conclusion: Ultrasonic piezosurgery proves to be a safe and effective device for performing surgical procedures in dentistry, due to the protection of soft tissues, better hemostasis, decreased noise and vibration, decreased stress and fear of the patient, minimizing damage to structures noble as nervous vascular bundles.Introducción: La piezoelectricidad apareció a mediados del siglo XIX, el piezo ultrasónico consiste en el uso de un dispositivo ultrasónico, capaz de cortar tejido óseo mineralizado, sin dañar el tejido blando, lo cual es una de sus principales ventajas frente a las técnicas convencionales con taladros, sierras e instrumental. Objetivo: Este estudio tiene como objetivo realizar una revisión de la literatura sobre los diversos usos de la piezoelectricidad en odontología. Materiales y métodos: Para la elaboración de este artículo se realizó una revisión narrativa de la literatura, utilizando las bases de datos: PUBMED / MEDLINE, Google académico, Scielo, Revodonto, LILACS, BBO y Portal CAPES. Se seleccionaron artículos que abordaron los temas de cirugía ecográfica, piezocirugía, técnicas quirúrgicas alternativas y dispositivos quirúrgicos de vibración. Discusión: Según la teoría de los hermanos Curie, el efecto piezoeléctrico se produce mediante el uso de tipos específicos de cerámicas y cristales como el cuarzo, que sufren deformaciones cuando pasa una corriente eléctrica a través de ellos, dando como resultado oscilaciones de frecuencia ultrasónica y al aplicarse. si en una superficie estas vibraciones mecánicas generan una ruptura en la cohesión molecular provocan cavitaciones en esta superficie. Conclusión: La piezocirugía ultrasónica demuestra ser un dispositivo seguro y eficaz para realizar procedimientos quirúrgicos en odontología, debido a la protección de los tejidos blandos, mejor hemostasia, disminución del ruido y vibración, disminución del estrés y miedo del paciente, minimizando el daño a las estructuras. noble como haces vasculares nerviosos.Introdução: A piezoeletricidade surgiu em meados do século XIX, o piezo ultrassônico consiste na utilização de um aparelho ultrassônico, capaz de cortar tecido ósseo mineralizado, sem lesionar o tecido mole sendo essa uma de suas principais vantagens quando comparado às técnicas convencionais com brocas, serras e instrumentais. Objetivo: Este estudo tem como objetivo, realizar uma revisão de literatura acerca das diversas utilizações do piezoeletricidade na odontologia. Materiais e Metódos: Para a elaboração desse artigo foi realizada uma revisão narrativa da literatura, utilizando as bases de dados: PUBMED/ MEDLINE, Google acadêmico, Scielo, Revodonto, LILACS, BBO e Portal CAPES. Foram selecionados artigos que abordassem os assuntos cirurgia com ultrassom, piezocirúrgico, técnicas cirúrgicas alternativas e aparelhos cirúrgicos de vibração. Discussão: De acordo com a teoria dos irmãos Curie o efeito piezoelétrico acontece através do uso de tipos específicos de cerâmicas e cristais como o quartzo, os quais sofrem deformações quando uma corrente elétrica passa através deles, resultando em oscilações de frequência ultrassônica e quando aplica-se sobre uma superfície estas vibrações mecânicas geram uma ruptura na coesão molecular provocam cavitações nesta superfície. Conclusão: O piezocirúrgico ultrassônico demonstra ser um dispositivo seguro e eficaz para a realização de procedimentos cirúrgicos na odontologia, devido a proteção aos tecidos moles, melhor hemostasia, diminuição do ruído e vibração, diminuição do estresse e medo do paciente, minimização de dano a estruturas nobres como feixes vásculo nervoso

Fractional order dynamical systems and its applications

This article illustrates several applications of fractional calculus (FC) in science and engineering. It has been recognized the advantageous use of this mathematical tool in the modeling and control of many dynamical systems. In this perspective, this paper investigates the use of FC in the following fields: Controller tuning; Electrical systems; Traffic systems; Digital circuit synthesis; Evolutionary computing; Redundant robots; Legged robots; Robotic manipulators; Nonlinear friction; Financial modeling.N/

Dental and composite resin discoloration induced by different hydraulic calcium silicate-based cements: two-year in vitro assessment

Few long-term studies assess the discoloration induced by hydraulic calcium silicate-based cement on dental structures. In addition, as far as we know, no long-term study has assessed the discoloration induced by these cement on composite resin. Objective: This in vitro study aimed to assess, during a period of two years, the discoloration potential of different hydraulic calcium silicate-based cements (hCSCs) on the enamel/dentin structure and composite resin restoration. Methodology: A total of 40 enamel/dentin discs were obtained from bovine incisors, and 40 composite resin discs (10 mm in diameter × 2 mm thick) were fabricated. A 0.8 mm-deep cavity was made in the center of each disc and filled with the following hCSCs (n=10): Original MTA (Angelus); MTA Repair HP (Angelus); NeoMTA Plus (Avalon); and Biodentine (Septodont). An initial color measurement was performed (T0 - baseline). After 7, 15, 30, 45, 90, 300 days, and two years, new color measurements were performed to determine the color (ΔE00), lightness (ΔL’), chroma (ΔC’), hue differences (ΔH’), and whiteness index (WID). Results: For enamel/dentin, the ΔE00 was significant among groups and periods (p&lt;0.05). NeoMTA Plus had the greatest ΔE00. The NeoMTA Plus group had the greatest ΔE00 after two years for composite resin. Significant reduction in lightness was observed for all groups after two years (p&lt;0.05). The most significant WID values were observed after 30 days for Biodentine (enamel/dentin) and MTA Repair HP groups (composite resin) (p&lt;0.05). Conclusions: The hCSCs changed the colorimetric behavior of both substrates, leading to greater darkening over time. The Bi2O3 in the Original MTA seems relevant in the short periods of color change assessment