Immunoblot analysis of antigens associated with Haemophilus ducreyi using serum from immunised rabbits.

Sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting were used to characterise isolates of Haemophilus ducreyi. Isolates of H ducreyi were heterogeneous in protein composition, but isolates from single outbreaks appeared similar both in protein profiles and antigenic analysis. Rabbits immunised with H ducreyi responded with a vigorous humoral immune response in which multiple antigenic polypeptides were detected. The most prominent antigens had molecular masses of 67, 42, 22.5, and 20 kilodaltons

Protocols for calibrating multibeam sonar

Author Posting. © Acoustical Society of America, 2005. This article is posted here by permission of Acoustical Society of America for personal use, not for redistribution. The definitive version was published in Journal of the Acoustical Society of America 117 (2005): 2013-2027, doi:10.1121/1.1869073.Development of protocols for calibrating multibeam sonar by means of the standard-target method is documented. Particular systems used in the development work included three that provide the water-column signals, namely the SIMRAD SM2000/90- and 200-kHz sonars and RESON SeaBat 8101 sonar, with operating frequency of 240 kHz. Two facilities were instrumented specifically for the work: a sea well at the Woods Hole Oceanographic Institution and a large, indoor freshwater tank at the University of New Hampshire. Methods for measuring the transfer characteristics of each sonar, with transducers attached, are described and illustrated with measurement results. The principal results, however, are the protocols themselves. These are elaborated for positioning the target, choosing the receiver gain function, quantifying the system stability, mapping the directionality in the plane of the receiving array and in the plane normal to the central axis, measuring the directionality of individual beams, and measuring the nearfield response. General preparations for calibrating multibeam sonars and a method for measuring the receiver response electronically are outlined. Advantages of multibeam sonar calibration and outstanding problems, such as that of validation of the performance of multibeam sonars as configured for use, are mentioned.Support by the National Science Foundation through Award No. OCE-0002664, NOAA through Grant No. NA97OG0241, and the Cooperative Institute for Climate and Ocean Research (CICOR) through NOAA Contract No. NA17RJ1223 is acknowledged

Comparison of granulocyte colony-stimulating factor (G-CSF)--mobilized peripheral blood progenitor cells and G-CSF--stimulated bone marrow as a source of stem cells in HLA-matched sibling transplantation

AbstractHLA-identical bone marrow or stem cell transplantation from a sibling is the preferred treatment for patients with chronic myelogenous leukemia, bone marrow failure syndromes, relapsed acute leukemia, and specific inborn errors of metabolism. Several groups have shown that granulocyte colony-stimulating factor (G-CSF)--mobilized peripheral blood progenitor cells (PBPCs) obtained from HLA-matched siblings are effective in reconstitution of marrow function after marrow ablative conditioning therapy. To evaluate whether G-CSF treatment before bone marrow harvest leads to enhanced recovery of PBPC counts and recovery from limited graft-versus-host disease (GVHD), we assessed the outcome of a sequential cohort of patients treated identically and then given either G-CSF--mobilized PBPCs or G-CSF--stimulated bone marrow from HLA-identical siblings. We show that the time to neutrophil engraftment is identical in the 2 cohorts, whereas platelet engraftment is earlier with the use of PBPCs. The incidence of acute GVHD was decreased, and that of chronic GVHD significantly decreased, in the group receiving bone marrow. Overall survival was not different between the 2 groups. Thus, G-CSF--stimulated bone marrow offers a source of stem cells that allows for early neutrophil engraftment with a decreased risk of GVHD.Biol Blood Marrow Transplant 2000;6(4A):434-40

Effects of morphine, nalorphine and naloxone on neocortical release of acetylcholine in the rat

The effects of morphine (10 mg/kg), nalorphine (1 and 10 mg/kg), and naloxone (1 mg/kg) were studied on the neocortical release of acetylcholine (ACh) in midpontine pretrigeminal transected rats. Morphine and, to a lesser extent, nalorphine decreased ACh release. Naloxone was ineffective alone but antagonized the action of morphine.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46384/1/213_2004_Article_BF00422643.pd

Workflows and individual differences during visually guided routine tasks in a road traffic management control room

Road traffic control rooms rely on human operators to monitor and interact with information presented on multiple displays. Past studies have found inconsistent use of available visual information sources in such settings across different domains. In this study, we aimed to broaden the understanding of observer behaviour in control rooms by analysing a case study in road traffic control. We conducted a field study in a live road traffic control room where five operators responded to incidents while wearing a mobile eye tracker. Using qualitative and quantitative approaches, we investigated the operators’ workflow using ergonomics methods and quantified visual information sampling. We found that individuals showed differing preferences for viewing modalities and weighting of task components, with a strong coupling between eye and head movement. For the quantitative analysis of the eye tracking data, we propose a number of metrics which may prove useful to compare visual sampling behaviour across domains in future

Formation of nonspecific precipitants in sera by counterimmunoelectrophoresis.

Formation of nonspecific precipitants in sera in different kinds of agarose and batches of barbital buffer by routine counterimmunoelectrophoresis was observed. These nonspecific precipitants consistently locating at the cathodal side of the well upon counterimmunoelectrophoresis can lead to the incorrect interpretation that a specific antibody is present in the patient's serum against the antigens tested. This observation indicates the importance of including a negative control (patient's serum alone) with each individual sample tested by counterimmunoelectrophoresis for the detection of specific antibody to reduce false-positives