Transcriptional regulation of prostate kallikrein-like genes by androgen.

Using gene-specific synthetic oligonucleotides the expression and regulation of kallikrein-like genes in the human prostatic cancer cell line LNCaP were studied. Prostate-specific antigen (PSA) and human glandular kallikrein (hGK-1) together constitute a subfamily of serine proteases exclusively produced in the human prostate. RNA analysis revealed that both genes are expressed in LNCaP cells with PSA basal levels being 2-fold higher than hGK-1 levels. Both mRNAs are induced over a period of 24 h in the presence of 3.3 nM of the synthetic androgen mibolerone. Stimulation of PSA RNA is about 5- fold,whereas hGK-1 stimulation is less pronounced. Nuclear run-on analysis revealed that androgen induction of kallikrein-like genes in LNCaP cells is a rapid event (c3 h) occurring at the level of transcription initiation. Treatment of cells with cycloheximide demonstrates that, while PSA/hGK-1 basal transcription strictly depends on continuous protein synthesis, transcriptional induction by androgen does not. This suggests the direct involvement of the androgen receptor in the induction process independent of additional labile protein factors necessary for kallikrein basal transcription. A binding motif is present in the PSA and hGK-1 promoters, closely resembling the consensus sequence for steroidresponsive elements. The androgen antagonist cyproterone acetate was also able to stimulate transcription of kallikrein-like genes in LNCaP cells. In contrast, androgen-dependent transcriptional suppression of the protooncogene c-myc was strongly counteracted by cyproterone acetate. Thus, antiandrogens act differentially on androgen-regulated prostate-specific (PSA, hGK-1) and growthrelated (c-myc) gene expression in LNCaP cells

Generalized prime models

A prime model O of some complete theory T is a model which can be elementarily imbedded into any model of T (cf. Vaught [7, Introduction]). We are going to replace the assumption that T is complete and that the maps between the models of T are elementary imbeddings (elementary extensions) by more general conditions. T will always be a first order theory with identity and may have function symbols. The language L(T) of T will be denumerable. The maps between models will be so called F-maps, i.e. maps which preserve a certain set F of formulas of L(T) (cf. I.1, 2). Roughly speaking a generalized prime model of T is a denumerable model O which permits an F-map O→M into any model M of T. Furthermore O has to be "generated” by formulas which belong to a certain subset G of

Levél a cementeshordóban

葉山嘉樹『セメント樽の中の手紙』Translations　Hungarian Magya

Learning By Teaching: A Cultural Historical Perspective On A Teacher’s Development

How can teacher development be characterised? In this paper we offer a conceptualisation of teacher development as the enhancement of knowledge and capabilities to function in the activity of a teacher and illustrate with a case study. Our analytic focus is on the development of a science teacher, David, as he engaged in an innovative, collaborative project on learning photonics at a metropolitan secondary school in Australia. Three dimensions of development emerged: technical confidence and competence, pedagogical development and personal agency. We explore the transformative effects of intrapersonal tensions within the teacher’s constitution of his role in the emerging community of enquiry — positioning him in turn as learner, instructor and facilitator. We view the context for David’s actions as a complex and dynamic system and interpret David’s development as arising from his responses to the differences in his emerging roles in the project

Orrpolyposis kezelésére szolgáló gombaellenes hatóanyag tartalmú készítmény fejlesztésének klinikai és analitikai vonatkozásai

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Stakeholders of the Online Pharmaceutical Market

During the past two decades, the pharmacy supply chain has developed a new segment besides traditional “brick and mortar” pharmacies. The expansion of the internet, consumer experience in online purchases, the ease of mail order trade, and distance selling have facilitated the growth of the internet pharmacy landscape. Changes in health-seeking behavior, patient empowerment, and openness to self-diagnosis and self-treatment have also contributed to the phenomenon and were further facilitated by the pandemic. Various types of online medicinal product sellers have been published previously, however, authors have classified online pharmacies mainly according to legality and patient safety considerations. As online pharmacies show great diversity, no distinct categories can be specified, rather pharmacies on the web can be categorized by multiple aspects. Admittedly, consumer preferences, regulatory environment, and legitimacy of operation are key influencing factors. In this chapter, key aspects of categorization and nomenclature are discussed to profile different vendors on the internet

Phase Operator Problem and Macroscopic Extension of Quantum Mechanics

To find the Hermitian phase operatorof a single-mode electromagnetic field in quantum mechanics, the Schroedinger representation is extended to a larger Hilbert space augmented by states with infinite excitation by nonstandard analysis. The Hermitian phase operator is shown to exist on the extended Hilbert space. This operator is naturally considered as the controversial limit of the approximate phase operators on finite dimensional spaces proposed by Pegg and Barnett. The spectral measure of this operator is a Naimark extension of the optimal probability operator-valued measure for the phase parameter found by Helstrom. Eventually, the two promising approaches to the statistics of the phase in quantum mechanics is unified by means of the Hermitian phase operator in the macroscopic extension of the Schroedinger representation.Comment: 26 pages, LaTeX, no figures, to appear in Ann. Phys. (N.Y.