Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication

Chromosomes of Brassica oleracea (2n=18) were dissected from the resynthesized amphidiploid B. napus Hakuran by repeated backcrosses to B. campestris (2n=20), creating a series of monosomic alien chromosome addition line plants (2n=21). Using morphological, isozyme and restriction fragment length polymorphism markers (RFLPs), 81 putative loci were identified. Of nine possible synteny groups, seven were represented in the 25 monosomic addition plants tested. Sequences homologous to 26% of the 61 DNA clones utilized (80% were cDNA clones) were found on more than one synteny group, indicating a high level of gene duplication. Anomalous synteny associations were detected in four 2n=21 plants. One of these plants showed two markers from one B. oleracea chromosome associated with a second complete B. oleracea synteny group, suggesting translocation or recombination between non-homologous chromosomes in Hakuran or the backcross derivatives. The other three 2n=21 plants each contained two or more B. oleracea synteny groups, suggesting chromosome substitution.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47572/1/438_2004_Article_BF00265054.pd

Dr. Scott Figdore - Overview of herbarium specimen efforts at UIU over the past 160 years.

“The presentation will focus on the importance of documenting current plant species diversity at the county-level,” Figdore said. “I will also share preliminary information from our efforts over the past 20 years to create a plant inventory and herbarium collection from some of Fayette County’s beautiful natural areas.” The Upper Iowa University Herbarium was renamed to honor Dr. Charles Coleman (C. C.) Parker, UIU’s first professor of natural sciences and Fayette’s first town doctor, who also served in the 12th Iowa Infantry Regiment (University Recruits) during the Civil War as a regimental surgeon. Parker collected nearly 500 herbarium specimens in the 1860’s and 1870’s, including several rare specimens no longer typically found in the region. Thanks to a generous gift from Dr. James D. Parker, the great-grandson of C. C. Parker, Figdore has been working on restoring the historical herbarium collections of C.C. Parker and others, as well as on creating a more recent herbarium collection, so that the C. C. Parker Herbarium can be recognized and utilized for its archival and historical significance by the scientific community. With over 30 years of service at UIU, Figdore served as the inaugural dean of the School of Science and Mathematics for four years before returning to fulltime faculty duties. The Robert L. Fox Endowed Chair of Science was initiated by a generous gift from members of the Parker, Fox and Goebel families. The first recipient of the Robert L. Fox Endowed Chair was the late Professor Emeritus Lew Churbuck. At the time of his honor, Churbuck was the curator of the Upper Iowa University Herbarium. Figdore assumed the duties of the curator position after professor Churbuck’s retirement in 2007.Baker-Hebron Hall[Title], Upper Iowa University Digital Archives, [Reference URL]. See 'About' page for more information;Born digital

Nitrification bioaugmentation in mainstream flocculent activated sludge systems using sidestream aerobic granular sludge

Thesis (Ph.D.)--University of Washington, 2017-12Increased nitrification capacity in flocculent activated sludge wastewater treatment systems may be required to meet lower effluent ammonia-nitrogen (NH3-N) concentration limits, to handle higher influent flows, and/or for conversion to nitrogen removal. A novel approach to increase nitrification capacity in flocculent activated sludge systems is through bioaugmentation with nitrifying granular sludge grown in a sidestream treatment reactor fed anaerobic digester dewatering centrate. Granules from the sidestream reactor are added to the mainstream flocculent activated sludge system to increase the nitrifying biomass concentration without increasing aeration tank volume. The granular sludge solids retention time (SRT) can be decoupled from the low SRT of the flocculent sludge by selective granule retention due to the larger size and faster settling velocity of granules. Retention and accumulation of the granule biomass results in a high volumetric nitrification capacity. The main research goal was to demonstrate viability of the granular sludge nitrification bioaugmentation process and address fundamental scientific and process application considerations pertaining to sidestream and mainstream treatment performance, nitrification kinetics, and granule biopopulations. Different types of nitrifying granules were grown on simulated centrate and evaluated for mainstream bioaugmentation potential based on granule nitrification capacity yield and physical characteristics favorable for mainstream retention. Granules performing a) nitrification only and b) nitrification, denitrification and enhanced biological phosphorus removal showed the greatest potential and were further studied in centrate treatment systems. Successful growth of both granule types was obtained with centrate feeding and consistent production of waste granular sludge for bioaugmentation. In separate bioaugmentation tests using the different granule types, addition and selective retention of granules sustained nitrification and allowed nitrogen removal by denitrification in non-nitrifying flocculent activated sludge. Mainstream effluent NH3-N concentrations near 1 mg/L were achieved at 12 degrees Celsius. Granule removal at the end bioaugmentation immediately resulted in near-complete loss of nitrification. Molecular microbial analyses showed changes to the granule microbial community composition in mainstream treatment and low abundance of ammonia oxidizing bacteria in waste flocculent sludge. The overall results of this research demonstrate that nitrification bioaugmentation with granular activated sludge from sidestream centrate treatment can greatly intensify treatment capability of flocculent activated sludge systems by enabling nitrification and nitrogen removal in otherwise non-nitrifying flocculent activated sludge

Additional file 1 of Comparison of intact protein and digested peptide techniques for high throughput proteotyping of ApoE

Additional file 1: Table S1. Sciex 7500 source conditions. Table S2. Sciex 7500 parameters. Table S3. Exploris 480 parameters. Fig. S1. Box plots of the distribution of IS corrected peak areas derived from LC-MS/MS measurement of the ApoE peptides necessary for proteotyping for only the subset of samples selected for subsequent analyses. Fig. S2. Representative chromatograms of the intact protein XICs. Fig. S3. Box plots of the distribution of peaks areas from the integration of chromatographic peaks when performing LC-MS measurement of intact ApoE. Fig. S4. Box plots of the distribution of deconvoluted spectral signal intensities when performing LC-MS measurement of intact ApoE

Effect of cationic surfactant addition on the electrokinetics and stability of silica/kaolinite suspensions in copper hydrometallurgy conditions

JVE thank to MAINI - UCN (Scientific Equipment Unit - Universidad Catolica del Norte) and CEITSAZA - UCN (Technological Center for Research on Water in the Desert - Universidad Catolica del Norte) for supporting this work. AVD and SA acknowledge financial support from the Spanish Institutions: Ministerio de Ciencia, Innovacion y Universidades (GC2018-098770-B-I00) , and Junta de Andalucia (BF-FQM-141-UGR18) .The presence of small particles at operating conditions of the hydrometallurgy process forming the crud is a complicated issue. These particles, most often silica and clays, remain in the suspension or float to the top. A potential solution for their elimination could be to promote the sedimentation of the solids by the addition of surfactant. However, such a procedure in these complex systems, with different kinds and sizes of particles, requires the evaluation of the composition and optimal dose of surfactant. In this work, we explore the use of dynamic electrophoretic mobility for indirectly evaluating the adsorption of CTAB on silica/kaolinite mixtures at low pH, high ionic strength, and particle concentration above 3%. The electroacoustic technique (ESA) shows to be suitable to predict the effect of surfactant addition on the individual particles of silica and kaolinite and mixtures of them. The conclusions were in agreement with sizes and sedimentation rate determinations. Moreover, scanning electron microscope (SEM) images confirm the evolution of aggregates and the formation of structures between particles by the addition of CTAB. Since the final aim is the identification of the optimal doses of surfactant in which the described suspensions flocculate and separate from the continuous phase, this investigation can potentially contribute to solve operational problems associated to the generation of crud in the hydrometallurgy process.MAINI - UCN (Scientific Equipment Unit - Universidad Catolica del Norte)CEITSAZA - UCN (Technological Center for Research on Water in the Desert - Universidad Catolica del Norte)Spanish Institution: Ministerio de Ciencia, Innovacion y Universidades GC2018-098770-B-I00Spanish Institution: Junta de Andalucia BF-FQM-141-UGR1