THE METROLOGY AND THE BIM APPROACH: A NEW COGNITIVE PARADIGM ABOUT THE ANCIENT CONSTRUCTION

Abstract. Nowadays, some important issues in the BIM field and research are still open, and are not deeply known and really understood yet by all the BIM users and approaches; unfortunately, this critical situation implies, often, incorrect approaches and ineffective applications, especially using BIM for existing historical building, where the object oriented approach has so many limitations. In fact, the current laser scanning technology can detect just a cloud of points that is considered "non-intelligent" according to the BIM method and meaning. The paper presents an important case study: the Cathedral of Piazza dei Miracoli (Miracles Square) in Pisa. The method we propose consists of an innovative procedure combining in a two-way manner the correspondence levels between metrology and ontology of the constructive components. The paper proves that is not important to obtain the best possible level of modeling performance. We need a specific BIM approach, for "a specific building", according with "a specific project". We believe that it is precisely in this capacity of matching between metrological and constructive knowledge (mediated by computer graphics technology) that it is possible to identify the response to an important programmatic indication in the BIM field: "how much BIM?". This sentence was explained by Charles Eastman (recognized worldwide as the father of BIM) during his "lectio magistralis" at the Master BIM of the University of Pisa (www.unibim.it). We guess this is the way to be free from any pre-established intervention standards, and so to be adapting to a specific construction for a specific intervention's level using specific resources

Digital methodologies for existing buildings emerging education and training for professionals

Processes such as Building Information Modeling and, more generally, those involving the digitization of the built environment whose BIM is one of the possible expressions, are becoming increasingly pervasive in many different practices, from the design activities to the building site and management. Professional skills and working experience have now to be fostered by specific training on new methodologies concerning virtual replicas of existing domains, to explore the possibilities offered by digital interactions with Smart Heritage artifacts. This paper delves into the outcomes from the BIM Master Program held at the University of Pisa since 2016, presenting the results of the application of novel teaching techniques and topics related to the digitization of the built historic environment for the design preservation of Cultural Heritage monuments or sites

MATERIALI DI ARCHITETTURA CONTEMPORANEA

Il rapporto tra linguaggi e materiali ha sempre avuto un rilevante significato nella storia dell'Architettura. Questo testo intende indagare, attraverso un'analisi congiunta, il rapporto tra alcuni materiali di recente affermazione e lo sviluppo delle Architetture in cui sono stati utilizzati, al fine di individuare le possibili ricadute nella tecnica dell'architettura contemporanea. Per interpretare e documentare criticamente il rapporto d'interazione fra l'elemento tecnologico e l'idea progettuale, sono stati individuati materiali esemplificativi di alcuni attuali sviluppi della pratica professionale e della ricerca come quelli polimerici, computazionali e "viventi"

Shoreline changes near river mouth: case study of Sant'Agata River (Reggio Calabria, Italy)

The analysis of shoreline changes is very important for coastal planning and management. In territories such as Calabria (Italy), characterized by significant anthropogenic pressures and various eroded coasts, the knowledge of the shoreline changes, and the factors that influence them, is necessary for management and planning of coastal areas. In fact, shoreline position is one of the most important indicators of coastal dynamics. From this point of view recent advances in remote sensing and GIS techniques allow to estimate with great precision the shoreline changes over the years. The paper analyzes the shoreline changes near the mouth of the Sant'Agata River (Reggio Calabria, Italy), carried out through the comparison of various cartography data. Furthermore, the paper analyzes the main factors influencing the coastal dynamics in order to identify possible correlation between these factors and the shoreline changes. The analysis of these factors shows that, in this case study, the rainfall regime has a considerable influence on shoreline change. The methodology described in this paper is particularly useful for better understanding the factors that most influence the coastal balance and, therefore, is applicable to many contexts which are similar to the Sant'Agata river mouth

Visualizing the life of mRNA in T cells

Correspondence: Monika Wolkers ([email protected]) T cells release ample amounts of cytokines during infection. This property is critical to prevent pathogen spreading and persistence. Nevertheless, whereas rapid and ample cytokine production supports the clearance of pathogens, the production must be restricted in time and location to prevent detrimental effects of chronic inflammation and immunopathology. Transcriptional and post-transcriptional processes determine the levels of cytokine production. How these regulatory mechanisms are interconnected, and how they regulate the magnitude of protein production in primary T cells is to date not well studied. Here, we highlight recent advances in the field that boost our understanding of the regulatory processes of cytokine production of T cells, with a focus on transcription, mRNA stability, localization and translation

Quercetin elevates p27Kip1 and arrests both primary and HPV16 E6/E7 transformed human keratinocytes in G1

Our previous work with primary bovine fibroblasts demonstrated that quercetin, a potent mutagen found in high levels in bracken fern (Pteridium aquilinum), arrested cells in G1 and G2/M, in correlation with p53 activation. The expression of bovine papillomavirus type 4 (BPV-4) E7 overcame this arrest and lead to the development of tumorigenic cells lines (Beniston et al., 2001). Given the possible link between papillomavirus infection, bracken fern in the diet and cancer of the upper gastrointestinal (GI) tract in humans, we investigated whether a similar situation would occur in human cells transformed by human papillomavirus type 16 (HPV-16) oncoproteins. Quercetin arrested primary human foreskin keratinocytes in G1. Arrest was linked to an elevation of the cyclin-dependent kinase inhibitor (cdki) p27Kip1. Expression of the HPV16 E6 and E7 oncoproteins in transformed cells failed to abrogate cell cycle arrest. G1 arrest in the transformed cells was also linked to an increase of p27Kip1 with a concomitant reduction of cyclin E-associated kinase activity. This elevation of p27Kip1 was due not only to increased protein half-life, but also to increased mRNA transcription

Stratus 9/VOCALS ninth setting of the Stratus Ocean Reference Station & VOCALS Regional Experiment

The Ocean Reference Station at 20°S, 85°W under the stratus clouds west of northern Chile is being maintained to provide ongoing climate-quality records of surface meteorology; air-sea fluxes of heat, freshwater, and momentum; and of upper ocean temperature, salinity, and velocity variability. The Stratus Ocean Reference Station (ORS Stratus) is supported by the National Oceanic and Atmospheric Administration’s (NOAA) Climate Observation Program. It is recovered and redeployed annually, with cruises that have come between October and December. During the 2008 cruise on the NOAA ship Ronald H. Brown to the ORS Stratus site, the primary activities were recovery of the Stratus 8 WHOI surface mooring that had been deployed in October 2007, deployment of a new (Stratus 9) WHOI surface mooring at that site; in-situ calibration of the buoy meteorological sensors by comparison with instrumentation put on board by staff of the NOAA Earth System Research Laboratory (ESRL); and observations of the stratus clouds and lower atmosphere by NOAA ESRL. A buoy for the Pacific tsunami warning system was also serviced in collaboration with the Hydrographic and Oceanographic Service of the Chilean Navy (SHOA). The DART (Deep-Ocean Assessment and Reporting of Tsunami) carries IMET sensors and subsurface oceanographic instruments. A DART II buoy was deployed north of the STRATUS buoy, by personnel from the National Data Buoy Center (NDBC) Argo floats and drifters were launched, and CTD casts carried out during the cruise. The ORS Stratus buoys are equipped with two Improved Meteorological (IMET) systems, which provide surface wind speed and direction, air temperature, relative humidity, barometric pressure, incoming shortwave radiation, incoming longwave radiation, precipitation rate, and sea surface temperature. Additionally, the Stratus 8 buoy received a partial CO2 detector from the Pacific Marine Environmental Laboratory (PMEL). IMET data are made available in near real time using satellite telemetry. The mooring line carries instruments to measure ocean salinity, temperature, and currents. The ESRL instrumentation used during the 2008 cruise included cloud radar, radiosonde balloons, and sensors for mean and turbulent surface meteorology. Finally, the cruise hosted a teacher participating in NOAA’s Teacher at Sea Program.Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA17RJ1223 for the Cooperative Institute for Climate and Ocean Research (CICOR)

Restoration ecophysiology: an ecophysiological approach to improve restoration strategies and outcomes in severely disturbed landscapes

As human activities destroy and degrade the world's ecosystems at unprecedented scales, there is a growing need for evidence-based methods for ecological restoration if we are to preserve biodiversity and ecosystem services. Mining represents one of the most severe anthropogenic disturbances, often necessitating intensive intervention to restore the most basic attributes of native ecosystems. Despite examples of successful mine-site restoration, re-establishing native vegetation in these degraded landscapes remains a significant challenge. Plant ecophysiology-the study of the interactions between plants and the environment-can provide a useful framework for evaluating and guiding mine-site restoration. By understanding the physiological mechanisms that allow plants to establish and persist in these highly disturbed environments, practitioners may be able to improve restoration outcomes. Specifically, methods in plant ecophysiology can inform site preparation and the selection of plant material for restoration projects, aid in monitoring restoration progress by providing additional insight into plant performance, and ultimately improve our ability to predict restoration trajectories. Here, we review the challenges and benefits of integrating an ecophysiological perspective to mine-site restoration in Western Australia, a global hotspot of biodiversity and mining operations. Using case studies and examples from the region's diverse ecosystems, we illustrate how an ecophysiological approach can guide the restoration of some of the world's most severely disturbed landscapes. With careful selection of study species and traits and consideration of the specific environmental conditions and stressors within a site, the restoration ecophysiology framework outlined here has the potential to inform restoration strategies across ecosystems

Adult male circumcision as an intervention against HIV: An operational study of uptake in a South African community (ANRS 12126)

<p>Abstract</p> <p>Background</p> <p>To evaluate the knowledge, attitudes and beliefs about adult male circumcision (AMC), assess the association of AMC with HIV incidence and prevalence, and estimate AMC uptake in a Southern African community.</p> <p>Methods</p> <p>A cross-sectional biomedical survey (ANRS-12126) conducted in 2007-2008 among a random sample of 1198 men aged 15 to 49 from Orange Farm (South Africa). Face-to-face interviews were conducted by structured questionnaire. Recent HIV infections were evaluated using the BED incidence assay. Circumcision status was self-reported and clinically assessed. Adjusted HIV incidence rate ratios (aIRR) and prevalence ratios (aPR) were calculated using Poisson regression.</p> <p>Results</p> <p>The response rate was 73.9%. Most respondents agreed that circumcised men could become HIV infected and needed to use condoms, although 19.3% (95%CI: 17.1% to 21.6%) asserted that AMC protected fully against HIV. Among self-reported circumcised men, 44.9% (95%CI: 39.6% to 50.3%) had intact foreskins. Men without foreskins had lower HIV incidence and prevalence than men with foreskins (aIRR = 0.35; 95%CI: 0.14 to 0.88; aPR = 0.45, 95%CI: 0.26 to 0.79). No significant difference was found between self-reported circumcised men with foreskins and other uncircumcised men. Intention to undergo AMC was associated with ethnic group and partner and family support of AMC. Uptake of AMC was 58.8% (95%CI: 55.4% to 62.0%).</p> <p>Conclusions</p> <p>AMC uptake in this community is high but communication and counseling should emphasize what clinical AMC is and its effect on HIV acquisition. These findings suggest that AMC roll-out is promising but requires careful implementation strategies to be successful against the African HIV epidemic.</p

HIV Surveillance in a Large, Community-Based Study: Results from the Pilot Study of Project Accept (HIV Prevention Trials Network 043)

<p>Abstract</p> <p>Background</p> <p>Project Accept is a community randomized, controlled trial to evaluate the efficacy of community mobilization, mobile testing, same-day results, and post-test support for the prevention of HIV infection in Thailand, Tanzania, Zimbabwe, and South Africa. We evaluated the accuracy of in-country HIV rapid testing and determined HIV prevalence in the Project Accept pilot study.</p> <p>Methods</p> <p>Two HIV rapid tests were performed in parallel in local laboratories. If the first two rapid tests were discordant (one reactive, one non-reactive), a third HIV rapid test or enzyme immunoassay was performed. Samples were designated HIV NEG if the first two tests were non-reactive, HIV DISC if the first two tests were discordant, and HIV POS if the first two tests were reactive. Samples were re-analyzed in the United States using a panel of laboratory tests.</p> <p>Results</p> <p>HIV infection status was correctly determined based on-in country testing for 2,236 (99.5%) of 2,247 participants [7 (0.37%) of 1,907 HIV NEG samples were HIV-positive; 2 (0.63%) of 317 HIV POS samples were HIV-negative; 2 (8.3%) of 24 HIV DISC samples were incorrectly identified as HIV-positive based on the in-country tie-breaker test]. HIV prevalence was: Thailand: 0.6%, Tanzania: 5.0%, Zimbabwe 14.7%, Soweto South Africa: 19.4%, Vulindlela, South Africa: 24.4%, (overall prevalence: 14.4%).</p> <p>Conclusions</p> <p>In-country testing based on two HIV rapid tests correctly identified the HIV infection status for 99.5% of study participants; most participants with discordant HIV rapid tests were not infected. HIV prevalence varied considerably across the study sites (range: 0.6% to 24.4%).</p> <p>Trial Registration</p> <p>ClinicalTrials.gov registry number <a href="http://www.clinicaltrials.gov/ct2/show/NCT00203749">NCT00203749</a>.</p