Mapping the distribution of luminous and dark matter in strong lensing galaxies

We present the distribution of luminous and dark matter in a set of strong lensing (early-type) galaxies. By combining two independent techniques - stellar population synthesis and gravitational lensing - we can compare the baryonic and dark matter content in these galaxies within the regions that can be probed using the images of the lensed background source. Two samples were studied, extracted from the CASTLES and SLACS surveys. The former probes a wider range of redshifts and allows us to explore the mass distribution out to ~5Re. The high resolution optical images of the latter (using HST/ACS) are used to show a pixellated map of the ratio between total and baryonic matter. We find dark matter to be absent in the cores of these galaxies, with an increasing contribution at projected radii R>Re. The slopes are roughly compatible with an isothermal slope (better interpreted as an adiabatically contracted NFW profile), but a large scatter in the slope exists among galaxies. There is a trend suggesting most massive galaxies have a higher content of dark matter in the regions probed by this analysis.Comment: 10 pages, 6 figures. To appear in "Dark Galaxies and Lost Baryons", IAU244 conference proceeding

Mapping the Distribution of Luminous and Dark Matter in Strong Lensing Galaxies

We present the distribution of luminous and dark matter in a set of strong lensing (early-type) galaxies. By combining two independent techniques - stellar population synthesis and gravitational lensing - we can compare the baryonic and dark matter content in these galaxies within the regions that can be probed using the images of the lensed background source. Two samples were studied, extracted from the CASTLES and SLACS surveys. The former probes a wider range of redshifts and allows us to explore the mass distribution out to ~ 5Re. The high resolution optical images of the latter (using HST/ACS) are used to show a pixellated map of the ratio between total and baryonic matter. We find dark matter to be absent in the cores of these galaxies, with an increasing contribution at projected radii R Re. The slopes are roughly compatible with an isothermal slope (better interpreted as an adiabatically contracted NFW profile), but a large scatter in the slope exists among galaxies. There is a trend suggesting most massive galaxies have a higher content of dark matter in the regions probed by this analysi

Predicting spectral features in galaxy spectra from broad-band photometry

We explore the prospects of predicting emission line features present in galaxy spectra given broad-band photometry alone. There is a general consent that colours, and spectral features, most notably the 4000 A break, can predict many properties of galaxies, including star formation rates and hence they could infer some of the line properties. We argue that these techniques have great prospects in helping us understand line emission in extragalactic objects and might speed up future galaxy redshift surveys if they are to target emission line objects only. We use two independent methods, Artifical Neural Neworks (based on the ANNz code) and Locally Weighted Regression (LWR), to retrieve correlations present in the colour N-dimensional space and to predict the equivalent widths present in the corresponding spectra. We also investigate how well it is possible to separate galaxies with and without lines from broad band photometry only. We find, unsurprisingly, that recombination lines can be well predicted by galaxy colours. However, among collisional lines some can and some cannot be predicted well from galaxy colours alone, without any further redshift information. We also use our techniques to estimate how much information contained in spectral diagnostic diagrams can be recovered from broad-band photometry alone. We find that it is possible to classify AGN and star formation objects relatively well using colours only. We suggest that this technique could be used to considerably improve redshift surveys such as the upcoming FMOS survey and the planned WFMOS survey.Comment: 10 pages 7 figures summitted to MNRA

Stellar and total mass in early-type lensing galaxies

For 18 well-observed gravitationally lensed QSOs, we compare new non-parametric mass profiles for the lensing galaxies with stellar-population models derived from published HST photometry. The large volume of parameter space searched -- with respect to the possible star formation histories -- allows us to infer robust estimates and uncertainties for the stellar masses. The most interesting results are: (1) the transition from little or no dark matter in the inner regions (<r_e) to dark matter dominating on the ~5r_e scale (~20 kpc) is clearly seen in massive ellipticals; (2) Such a trend is not seen in lower-mass galaxies, so that the stellar content dominates the mass budget out to ~5r_e; (3) the radial gradient in the dark-matter fraction for these intermediate redshift galaxies agrees with published data on nearby galaxies. This result can help reconcile the discrepancies found in recent estimates of dark matter in elliptical galaxies using different techniques (e.g. Planetary nebulae versus X-ray). The observed trend suggests the stellar component in massive galaxies extends further out in terms of the dark matter scale radius.Comment: 4 pages, 4 figures, uses emulateapj. Accepted for publication in The Astrophysical Journal Letter

Functional expression of a penicillin acylase from the extreme thermophile Thermus thermophilus HB27 in Escherichia coli

Background: Penicillin acylases (PACs) are enzymes of industrial relevance in the manufacture of beta-lactam antibiotics. Development of a PAC with a longer half-life under the reaction conditions used is essential for the improvement of the operational stability of the process. A gene encoding a homologue to Escherichia coli PAC was found in the genome of the thermophilic bacterium Thermus thermophilus (Tth) HB27. Because of the nature of this PAC and its complex maturation that is crucial to reach its functional heterodimeric final conformation, the overexpression of this enzyme in a heterologous mesophilic host was a challenge. Here we describe the purification and characterization of the PAC protein from Tth HB27 overexpressed in Escherichia coli. Results: Fusions to a superfolder green fluorescent protein and differential membrane solubilization assays indicated that the enzyme remains attached through its amino-terminal end to the outer side of the cytoplasmic membrane of Tth cells. In order to overexpress this PAC in E. coli cells, a variant of the protein devoid of its membrane anchoring segment was constructed. The effect of the co-expression of chaperones and calcium supplementation of the culture medium was investigated. The total production of PAC was enhanced by the presence of DnaK/J and GrpE and even more by trigger factor and GroEL/ES. In addition, 10 mM calcium markedly improved both PAC specific and volumetric activities. Recombinant PAC was affinity-purified and proper maturation of the protein was confirmed by SDS-PAGE and MALDI-TOF analysis of the subunits. The recombinant protein was tested for activity towards several penicillins, cephalosporins and homoserine lactones. Hydrophobic acyl-chain penicillins were preferred over the rest of the substrates. Penicillin K (octanoyl penicillin) was the best substrate, with the highest specificity constant value (16.12 mM-1.seg-1). The optimum pH was aprox. 4 and the optimum temperature was 75 [DEGREE SIGN]C. The half-life of the enzyme at this temperature was 9.2 h. Conclusions: This is the first report concerning the heterologous expression of a pac gene from a thermophilic microorganism in the mesophilic host E. coli. The recombinant protein was identified as a penicillin K-deacylating thermozymeL.T., A.H. and J.B. acknowledge funding from the Spanish Ministry of Science (grants CIT 010000-2009-29, RyC2006-02441) and an institutional grant from Fundación Ramón Areces to CBMS

Sequence, structure, and binding site analysis of kirkiin in comparison with ricin and other type 2 rips

Kirkiin is a new type 2 ribosome-inactivating protein (RIP) purified from the caudex of Adenia kirkii with a cytotoxicity compared to that of stenodactylin. The high toxicity of RIPs from Adenia genus plants makes them interesting tools for biotechnology and therapeutic applications, particularly in cancer therapy. The complete amino acid sequence and 3D structure prediction of kirkiin are here reported. Gene sequence analysis revealed that kirkiin is encoded by a 1572 bp open reading frame, corresponding to 524 amino acid residues, without introns. The amino acid sequence analysis showed a high degree of identity with other Adenia RIPs. The 3D structure of kirkiin preserves the overall folding of type 2 RIPs. The key amino acids of the active site, described for ricin and other RIPs, are also conserved in the kirkiin A chain. Sugar affinity studies and docking experiments revealed that both the 1α and 2γ sites of the kirkiin B chain exhibit binding activity toward lactose and D-galactose, being lower than ricin. The replacement of His246 in the kirkiin 2γ site instead of Tyr248 in ricin causes a different structure arrangement that could explain the lower sugar affinity of kirkiin with respect to ricin

Luminous Red Galaxies in Simulations: Cosmic Chronometers?

There have been a number of attempts to measure the expansion rate of the universe at high redshift using Luminous Red Galaxies (LRGs) as "chronometers". The method generally assumes that stars in LRGs are all formed at the same time. In this paper, we quantify the uncertainties on the measurement of H(z) which arise when one considers more realistic, extended star formation histories. In selecting galaxies from the Millennium Simulation for this study, we show that using rest-frame criteria significantly improves the homogeneity of the sample and that H(z) can be recovered to within 3% at z~0.42 even when extended star formation histories are considered. We demonstrate explicitly that using Single Stellar Populations to age-date galaxies from the semi-analytical simulations provides insufficient accuracy for this experiment but accurate ages are obtainable if the complex star formation histories extracted from the simulation are used. We note, however, that problems with SSP-fitting might be overestimated since the semi-analytical models tend to over predict the late-time star-formation in LRGs. Finally, we optimize an observational program to carry out this experiment.Comment: 11 pages, 10 figures. Accepted to MNRAS

The Fundamental Surface of Quad Lenses

In a quadruply imaged lens system the angular distribution of images around the lens center is completely described by three relative angles. We show empirically that in the 3D space of these angles, spanning 180 x 180 x 90 degrees, quads from simple two-fold symmetric lenses of arbitrary radial density profile and arbitrary radially dependent ellipticity or external shear define a nearly invariant 2D surface. We give a fitting formula for the surface using SIS+elliptical lensing potential. Various circularly symmetric mass distributions with shear up to 0.4 deviate from it by typically, rms~0.1 deg, while elliptical mass distributions with ellipticity of up 0.4 deviate from it by rms~1.5 deg. The existence of a near invariant surface gives a new insight into the lensing theory and provides a framework for studying quads. It also allows one to gain information about the lens mass distribution from the image positions alone, without any recourse to mass modeling. As an illustration, we show that about 3/4 of observed galaxy-lens quads do not belong to this surface within observational error, and so require additional external shear or substructure to be modeled adequately.Comment: 24 pages, 9 figures, accepted to MNRA