A importância das newsletters na comunicação interna das empresas

Mestrado em Línguas e Relações EmpresariaisEsta dissertação de mestrado, que tem como tema principal a Importância da Newsletter na comunicação interna das empresas do setor da Indústria Transformadora Nacional com o C.A.E.1: 25720 – Fabricação de Fechaduras, Dobradiças e de Outras Ferragens (CAE REV.3), foi desenvolvida a partir de referenciais teóricos e da análise dos questionários e inquéritos feitos às empresas nacionais do setor mencionado. A dissertação, composta por 5 capítulos, fornece uma visão geral da newsletter enquanto meio de comunicação empresarial e explora os conceitos de cultura de empresa e comunicação interna. De seguida, descreve e enquadra a Indústria Transformadora Nacional. Por último, apresenta um estudo de campo que teve como objetivo principal avaliar a importância das newsletters na comunicação interna das empresas no setor da Indústria Transformadora Nacional com o C.A.E.1: 25720 – Fabricação de Fechaduras, Dobradiças e de Outras Ferragens (CAE REV.3). O estudo releva que as newsletters não são conhecidas pela maioria das empresas com o C.A.E.1: 25720 – Fabricação de Fechaduras, Dobradiças e de Outras Ferragens (CAE REV.3) na Indústria Transformadora Nacional. E, ainda que sejam importantes para a divulgação de informação e a consolidação da identidade das empresas, atendendo à estrutura de micro e pequenas empresas deste setor, a existência da newsletter não é justificada na maioria dos casos.The main subject of this master’s dissertation is the importance of newsletters in the internal communication of companies within the National Manufacturing Industry with the economic activity classification - C.A.E.1: 25720 – Production of Locks, Hinges and Others Hardware (CAE REV3). It was developed on the basis of theoretical readings and the analysis of data obtained through the application of a questionnaire and telephone interviews carried out with national companies belonging to the above mentioned Industry. The dissertation, containing 5 chapters, provides an overview of the newsletter as a means of business communication and explores the concepts of corporate culture and internal communication. It then describes and outlines the National Transformation Industry. Finally, it presents a study which aimed to assess the importance of newsletters in internal business communication in the National Transformation Industry sector with the C.A.E.1: 25720 – Manufacture of locks, hinges and other hardware (CAE REV.3). The study reveals that newsletters are not known by the majority of the companies within the National Manufacturing Industry with C.A.E.1: 25720 – Production of Locks, Hinges and Others Hardware (CAE REV3). Although newsletters are important to keep information flowing and consolidating the identity of businesses, given the micro and small size of the companies in this sector, their existence is not justified for most of the companies

Produção de carbonizados (biochar) por pirólise de lamas biológicas

Mestrado em Engenharia do AmbienteNo âmbito deste trabalho foi possível perceber como se processa a conversão termoquímica da biomassa, neste caso pirólise lenta, a que condições operatórias é possível obter biochar e quais as suas propriedades. As propriedades do biochar são significativamente influenciadas pelas características da matéria-prima e pelas condições de pirólise. Os resultados obtidos mostram que: a temperatura é um dos fatores que influencia o processo de pirólise e consequentemente as propriedades do biochar. Foi realizado um estudo em que com a mesma matéria-prima foram feitos ensaios a diferentes humidades (0%, 15%, 45% e 65%) e diferentes temperaturas (400ºC e 500ºC). Os resultados obtidos apresentaram diferenças significativas, sendo que a temperatura foi o parâmetro que mais influenciou estes resultados. Com o aumento da temperatura também foi possível visualizar um aumento considerável do teor de cinzas, pH e condutividade elétrica e diversas alterações nos rendimentos pirolíticos, desde a diminuição do rendimento do biochar com o aumento da temperatura. Fez-se também um estudo sobre a massa volúmica verdadeira, a massa volúmica a granel e a dispersão granulométrica do biochar das diferentes lamas. O biochar proveniente das lamas da instalação C foi o que apresentou valores de pH e de condutividade elétrica mais elevados, o que poderá estar relacionado com o elevado conteúdo em cinzas. Já o biochar da instalação A e B apresentara resultados semelhantes o que poderá também ser influenciado pelas características das lamas.In this work it was possible to understand how the thermochemical conversion of the biomass is processed, in this case slow pyrolysis, to which operating conditions it is possible to obtain biochar and what its properties. The properties of biochar are significantly influenced by the characteristics of the raw material and the pyrolysis conditions. The results show that: temperature is one of the factors that influence the pyrolysis process and consequently the biochar properties. A study was performed in which the same raw materials were tested at different humidities (0%, 15%, 45% and 65%) and at different temperatures (400ºC and 500ºC). The results obtained showed significant differences, and temperature was the parameter that most influenced these results. As the temperature increased, it was possible to visualize a considerable increase in the ash content, pH and electrical conductivity and several changes in the pyrolytic yield, from the decrease of the biochar yield with the increase in temperature. A study was also carried out on the true bulk density, the bulk density and the biochar dispersion of the different sludge. The biochar from the sludge from the C plant was the one with the highest values of pH and electrical conductivity, which may be related to the high ash content. On the other hand, the biochar of the installation A and B had similar results, which could also be influenced by the characteristics of the sludge

Atuação do profissional da contabilidade frente aos desafios da era digital: um enfoque no Sistema Público de Escrituração Fiscal Digital (SPED)

The accounting professional in the face of technological innovations in the digital era, assumed an importante place in the transmission of information, in order to intermediate that revenue versus taxprayer relationship. This research had as objective to eveluate the performance of professional in face of the challanges of digital era, giving focus to the The Public Digital Bookkeeping System – SPED in the management of organizations. In terms of methodological procedure is characterizes as a descriptive research, with a qualitative and quantitative approach, using a data survey through questionnaire, apllied to accounting professionals. In the face of the application of this method, the opinions of 50 accounting professionals were collected and analyzed, are parto of the universe of 249 units presents in João Pessoa – PB, they are distributed in Entrepreneurs, MEI and EIRELI. The results show that SPED is positively impacting the work of accounting professionals, requiring the constant search for updates to keep knowledge of the subject. The aaccountants also emphasized that they consider theis knowledge on the subject satisfactory and that it is essential to train employees in order to have greater reliability of the data.NenhumaO profissional contábil diante das inovações na era digital, assumiu um papel importante na transmissão de informações, de forma a intermediar a relação fisco x contribuinte. Esta monografia teve como objetivo avaliar a atuação do profissional frente aos desafios da era digital, dando enfoque ao Sistema público de escrituração digital - SPED no gerenciamento das organizações. Quanto aos procedimentos metodológicos, caracteriza-se como uma pesquisa descritiva, com abordagem qualitativa e quantitativa, utilizando-se de levantamento de dados por meio de questionário, aplicado aos profissionais contábeis. Diante da aplicação desse método, foram coletadas e analisadas as opiniões de 50 profissionais contábeis, que fazem parte do universo de 249 unidades presentes em João Pessoa – PB, no qual são distribuídas em Empresários, MEI e EIRELI. Os resultados obtidos mostram que o SPED está impactando positivamente no trabalho dos profissionais contábeis, exigindo a busca constante por atualização para manter o domínio sobre o assunto. Os contadores destacaram ainda, que consideram satisfatório o seu conhecimento sobre o assunto e que é essencial capacitar os colaboradores, afim de possuir maior fidedignidade dos dados

Assessing the bacterial community composition of bivalve mollusks collected in aquaculture farms and respective susceptibility to antibiotics

Research Areas: Infectious Diseases ; Pharmacology & PharmacyAquaculture is a growing sector, providing several products for human consumption, and it is therefore important to guarantee its quality and safety. This study aimed to contribute to the knowledge of bacterial composition of Crassostrea gigas, Mytilus spp. and Ruditapes decussatus, and the antibiotic resistances/resistance genes present in aquaculture environments. Two hundred and twenty-two bacterial strains were recovered from all bivalve mollusks samples belonging to the Aeromonadaceae, Bacillaceae, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Micrococcaceae, Moraxellaceae, Morganellaceae, Pseudomonadaceae, Shewanellaceae, Staphylococcaceae, Streptococcaceae, Vibrionaceae, and Yersiniaceae families. Decreased susceptibility to oxytetracycline prevails in all bivalve species, aquaculture farms and seasons. Decreased susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefotaxime, cefoxitin, ceftazidime, chloramphenicol, florfenicol, colistin, ciprofloxacin, flumequine, nalidixic acid and trimethoprim/sulfamethoxazole were also found. This study detected six qnrA genes among Shewanella algae, ten qnrB genes among Citrobacter spp. and Escherichia coli, three oqxAB genes from Raoultella ornithinolytica and blaTEM-1 in eight E. coli strains harboring a qnrB19 gene. Our results suggest that the bacteria and antibiotic resistances/resistance genes present in bivalve mollusks depend on several factors, such as host species and respective life stage, bacterial family, farm’s location and season, and that is important to study each aquaculture farm individually to implement the most suitable measures to prevent outbreaks.info:eu-repo/semantics/publishedVersio

Intimidade e saúde

L'intimité est précédée par des processus actifs, à travers le cycle de vie. Le lien affectif est établi principalement sur la relation entre attachement mère-enfant, et les comportements et styles de liens ont tendance à avoir un impact sur les relations entre adultes. Le but de cette recherche exploratoire vise à déterminer la transcription physique (conséquences sur la santé) des expériences insatisfaisantes de l'intimité. 60 étudiants (30 femmes, 30 hommes) âgés entre 22 et 27 ans, impliqué dans un jeu de relation amoureuse (durée minimale: 6 mois) ont été contactés pour répondre à une batterie d'instruments (variables d'intérêt: l'intimité, l'identité, concept de soi et des liens). Une bonne santé semble être associée à des valeurs élevées de l'interdépendance et de faibles niveaux de dépendance dans la relation. Dans ces jeunes l'intimité fondée sur la dépendance commis 14% de la santé mentale. L'impact d'une intimité malsaine limitées de près de 9% la santé physique des jeunes adultes en bonne santé.Intimacy is preceded by active processes across the life cycle. The emotional bond is established primarily on the attachment relationship of mother-infant, whose behavioral patterns and linking styles tend to be reflected on adult relationships. The purpose of this exploratory research aims to determine the physical transcription (health consequences) of unsatisfactory experiences of intimacy. 60 university students (30 female, 30 male) aged between 22 and 27 years, involved in a love relationship set (minimum duration: 6 months) were contacted to answer a battery of instruments (variables of interest: intimacy, identity, self-concept and linking). A good health seems to be associated with high values of interdependence and low levels of dependency in the relationship. In these young students intimacy based on dependence commits 14% of mental health. The impact of an unhealthy intimacy limits by nearly 9% physical health of young healthy adults.La intimidad es precedida por los procesos activos, a través del ciclo de vida. El vínculo afectivo se establece principalmente en la relación de apego madre-hijo, y patrones de comportamiento y estilos de los vínculos tienden a repercutir en las relaciones adultas. El propósito de esta investigación exploratoria es determinar la transcripción física (consecuencias para la salud) de la intimidad de experiencias insatisfactorias. Sesenta universitarios (30 mujeres, 30 varones) de edades comprendidas entre 22 y 27 años, que participan en una relación de amor (duración mínima: 6 meses) fueron contactados para responder a una batería de instrumentos (variables de interés: la intimidad, la identidad, el autoconcepto y la vinculación). Un buen estado de salud parece estar asociado con altos valores de interdependencia y bajos niveles de dependencia en la relación. En estos jóvenes la intimidad basada en la dependencia compromete 14% de la salud mental. El impacto de una intimidad insalubre limita casi 9% de la salud física de los adultos jóvenes sanos.A intimidade é antecedida de processos ativos, transversais ao ciclo de vida. O vínculo afetivo estabelece-se primariamente na relação de vinculação mãe-bebê, e os padrões comportamentais e estilos de vinculação tendem a repercutir-se nas relações adultas. O objetivo exploratório desta investigação pretende apurar a transcrição física (consequências na saúde) de vivências insatisfatórias de intimidade. Sessenta universitários (30 do sexo feminino; 30 do sexo masculino), com idades entre 22 e 27 anos, envolvidos numa relação amorosa fixa (duração mínima: 6 meses) foram contactados para responder a uma bateria de instrumentos (variáveis de interesse: intimidade, identidade, autoconceito e vinculação). Um bom estado de saúde parece associar-se a valores elevados de interdependência e baixos valores de dependência na relação amorosa. Nestes jovens a intimidade baseada na dependência compromete 14% da saúde mental. O impacto de uma intimidade pouco saudável limita em quase 9% a saúde física de jovens adultos saudáveis

Teresa Calçada em entrevista: ler é poder

Teresa Calçada, Comissária do Plano Nacional de Leitura 2027 (PNL2027), foi entrevistada para a AdolesCiência por três alunos da Escola Secundária D. Pedro V, de Lisboa – o Diogo Silva, a Joana Ferreira e a M.ª Inês Monteiro que frequentam o 11.º ano. A professora bibliotecária da Escola, Lígia Arruda, colaborou na revisão das questões e na transcrição da entrevista; a coordenadora interconcelhia da Rede de Bibliotecas Escolares, Margarida Toscano, apoiou os alunos na gravação da entrevista, que decorreu no espaço do PNL2027. Ao longo de cerca de meia hora, Teresa Calçada foi falando das novidades do PNL2027, do seu próprio percurso como leitora e de como pode ser a leitura a marcar a diferença ao nível da construção pessoal, social, profissional e de cidadania. Falou ainda do que na sua perspetiva é verdadeiramente importante para se aprender a ler, a ler bem, e do estatuto da leitura em sociedades onde a tecnologia e a imagem se tornaram fundamentais.Teresa Calçada, Comissioner of the Plano Nacional de Leitura 2027 (National Reading Plan 2027), was interviewed for AdolesCiência by three students of D. Pedro V High School, in Lisbon – Diogo Silva, Joana Ferreira and Maria Inês Monteiro, of the 11th grade. Lígia Arruda, the school librarian teacher, collaborated in the review of the issues and the interview’s transcription; the regional coordinator of the School Libraries Network, Margarida Toscano, supported the students in recording the interview, which took place in the PNL2027 premises. For about half an hour, Teresa Calçada spoke about PNL2027’s innovations, her own journey as a reader and how reading can make a difference on a personal, social, professional and citizenship level. She also talked about what she believes to be truly important to learn to read, properly read, and about the status of reading in modern societies, where technology and image have become fundamental.info:eu-repo/semantics/publishedVersio

Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocolitica

New point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on the cell surface was used as a target cell. After eight cycles of selection, the final aptamer pool was sequenced by high throughput sequencing using the Illumina Novaseq platform. The sequencing data, analyzed using the Geneious software, was aligned, filtered and demultiplexed to obtain the key nucleotides possibly involved in the target binding. The most promising aptamer candidate, Apt1, bound specifically to YadA with a dissociation constant (Kd) of 11 nM. Apt1 was used to develop a simple electrochemical biosensor with a two-step, label-free design towards the detection of YadA. The sensor surface modifications and its ability to bind successfully and stably to YadA were confirmed by cyclic voltammetry, impedance spectroscopy and square wave voltammetry. The biosensor enabled the detection of YadA in a linear range between 7.0 × 104 and 7.0 × 107 CFU mL−1 and showed a square correlation coefficient >0.99. The standard deviation and the limit of detection was ~2.5% and 7.0 × 104 CFU mL−1, respectively. Overall, the results suggest that this novel biosensor incorporating Apt1 can potentially be used as a sensitive POC detection system to aid the diagnosis of Y. enterocolitica infections. Furthermore, this simple yet innovative approach could be replicated to select aptamers for other (bacterial) targets and to develop the corresponding biosensors for their detection.This research is affiliated with the VibrANT project that received funding from the EU Horizon 2020 Research and Innovation Programme under the Marie Sklowdowska-Curie Grant, agreement no 765042. In addition, the authors acknowledge the financial support from Fundação para a Ciência e Tecnologia (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit and of LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechnaical Systems, LA/P/0029/2020.info:eu-repo/semantics/publishedVersio

Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA of Yersinia enterocolitica

New point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on the cell surface was used as a target cell. After eight cycles of selection, the final aptamer pool was sequenced by high throughput sequencing using the Illumina Novaseq platform. The sequencing data, analyzed using the Geneious software, was aligned, filtered and demultiplexed to obtain the key nucleotides possibly involved in the target binding. The most promising aptamer candidate, Apt1, bound specifically to YadA with a dissociation constant (Kd) of 11 nM. Apt1 was used to develop a simple electrochemical biosensor with a two-step, label-free design towards the detection of YadA. The sensor surface modifications and its ability to bind successfully and stably to YadA were confirmed by cyclic voltammetry, impedance spectroscopy and square wave voltammetry. The biosensor enabled the detection of YadA in a linear range between 7.0 × 104 and 7.0 × 107 CFU mL−1 and showed a square correlation coefficient >0.99. The standard deviation and the limit of detection was ~2.5% and 7.0 × 104 CFU mL−1, respectively. Overall, the results suggest that this novel biosensor incorporating Apt1 can potentially be used as a sensitive POC detection system to aid the diagnosis of Y. enterocolitica infections. Furthermore, this simple yet innovative approach could be replicated to select aptamers for other (bacterial) targets and to develop the corresponding biosensors for their detection.This research is affiliated with the VibrANT project that received funding from the EU Horizon 2020 Research and Innovation Programme under the Marie Sklowdowska-Curie Grant, agreement no 765042. In addition, the authors acknowledge the financial support from Fundação para a Ciência e Tecnologia (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit and of LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechnaical Systems, LA/P/0029/2020.info:eu-repo/semantics/publishedVersio

Etiology and incidence facial fractures in children and adults

SummaryFacial trauma has presented an increasing occurrence in the last four decades, due especially to the growth of accidents with automobiles as well as to the urban violence. Both of which continue being the main cause of such traumas. Aim: To evaluate the features of the population victim of facial trauma as to gender, age, occupation, origin, type of fracture and its cause. Design study: retrospective clinical with transversal cohort. Material and Method: Retrospective study consulting hospital registers of 513 patients victms of the facial trauma. Results: There was a higher incidence of facial trauma on men (84,9%), white (82,7) and with an average age of 29. Regarding occupation, the trauma was mostly occurred to students (16,6%) and Masons (11,2%). The jaw was the most affected place (35%), followed by zygoma (24%) and by the nose (23%), though most patients presented a single facial fracture (82,5%). Among the causes, accidents with automobiles (28,3%), aggressions (21%) and accidental fall s (19,5%) were the most common. Conclusions: Accidents with automobiles continue being the main cause of facial trauma, especially of multiple factures due to the great transmission of kinetic energy