Metabolomics application in maternal-fetal medicine

Metabolomics in maternal-fetal medicine is still an "embryonic" science. However, there is already an increasing interest in metabolome of normal and complicated pregnancies, and neonatal outcomes. Tissues used for metabolomics interrogations of pregnant women, fetuses and newborns are amniotic fluid, blood, plasma, cord blood, placenta, urine, and vaginal secretions. All published papers highlight the strong correlation between biomarkers found in these tissues and fetal malformations, preterm delivery, premature rupture of membranes, gestational diabetes mellitus, preeclampsia, neonatal asphyxia, and hypoxic-ischemic encephalopathy. The aim of this review is to summarize and comment on original data available in relevant published works in order to emphasize the clinical potential of metabolomics in obstetrics in the immediate future

Influence of age at weaning and nutritive value of weaning diet on growth performance and caecal traits in rabbits

[EN] To investigate the relationship between dietary nutrient concentration and weaning age on growth performance and caecal characteristics of rabbits, a trial was carried out on 64 litters (eight rabbits/litter) comparing two weaning ages (25 vs. 34 d) and two diets (HC: high concentrated and LC: low concentrated diet) offered to the kits from 18 to 34 d of age. At 34 d of age 54 animals per group were caged individually and all were fed the LC diet until 45 d of age. All animals were then fed a standard fattening diet (37% neutral detergent fiber, 3.4% ether extract and 18.0% crude protein on dry matter basis) until slaughter at 80 d. The digestibility trial was performed from 56 to 60 d of age on another group of 16 rabbits (8 per diet). During the experimental period (18-80 d of age), feed intake and animal weights were recorded. Caecal volatile fatty acids were measured at 45 and 80 d of age, while microbiological analysis was performed at 25 and 34 d on healthy suckling rabbits. Digestibility of dry matter, protein, neutral detergent fiber, fat and energy was higher in rabbits fed the HC diet (by 7.7, 7.4, 13.1, 26.7, 10.6%, respectively; P<0.001) than in those fed the LC diet. Growth rate and feed conversion ratio (FCR) from 18 to 34 d improved by 7.7 and 9.5% in rabbits fed HC in comparison to the LC diet, respectively (P¿0.061). Diet offered around weaning did not influence growth rate or FCR from 34 to 80 d of age. Rabbits weaned earlier increased feed intake (by 67.8%) from 25 to 34 d, but reduced growth rate (by 26.8%) compared to animals weaned at 34 d (P<0.001). Early weaning reduced live weight during the entire fattening period (P¿0.018), as well as the FCR (by 4%; P<0.001). Caecal pH was lower at 45 d of age in rabbits weaned at 25 d than in those weaned at 34 d (5.53 vs. 5.83; P=0.019). Treatments did not affect total caecal VFA concentration, but HC diet decreased caecal acetic concentration and increased butyric acid level compared to the LC diet at 45 d (P¿0.001). HC diet tended to reduce caecal counts of Enterobacteriacae (P=0.093), while it did not affect facultative anaerobic bacteria, E. Coli and Costridium spp. The LC diet increased at 25 d the number of Clostridium perfringens in comparison with the HC group at the same age and in comparison with animals fed both two diets at 34 d (P<0.001). The values of E. Coli and Enterobacteriaceae increased (P¿0.008) from 25 to 34 d of age, whereas that of Clostridium spp. decreased. Weaning at 25 d increased mortality from 18 to 34 d compared with rabbits weaned at 34 d (7.02 vs. 2.46%, P=0.017). However, during the fattening period (34-80 d) rabbits weaned earlier showed lower mortality (7.41 vs. 17.6%; P=0.024).This work has been realized within the CUNISVEZZ research project financed by the Agricultural Department of the Regione Lombardia as part of the 2004 research and development plan, d.g.r. 30/04/2004 n. VII/17326. The authors wish to thank Nicoletta Cesari for her technical assistance.Cesari, V.; Grilli, G.; Ferrazzi, V.; Toschi, I. (2009). Influence of age at weaning and nutritive value of weaning diet on growth performance and caecal traits in rabbits. World Rabbit Science. 17(4):195-205. https://doi.org/10.4995/wrs.2009.64419520517

Efficacy in the field of two anticoccidial vaccines for broilers

We compared two attenuated anticoccidial vaccines, administered to broilers by spray into the incubator (88,000 males and 210,100 females). Vaccine A container five species of Eimeria and vaccine B three. Zootechnical performance was similar in the two groups, with mean lesion scores no higher than 1; vaccine A caused only duodenal lesions, while vaccine B also caused typhlitis. Maximum oocyst count was 23,000/g feces at age 28 days with vaccine A and 38,000 at 21 days with vaccine B. Broilers vaccinated with vaccine B had more frequent enteric symptoms, and C. perfringens isolation

VP50.10: Predicting fetal and maternal complications with sFlt1/PlGF ratio in hypertensive disorders of pregnancy

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Microbiological and serological monitoring in hooded crow (Corvus corone cornix) in the Region Lombardia, Italy

The health status of 276 hooded crows (Corvus corone cornix) from various provinces of Lombardy was monitored for three years. Bacteriological examination detected E. coli (76%), Campylobacter jejuni (17%), Salmonella typhimurium (11.6%), Yersinia spp. (6.5%), Clamydophila abortus and C. psittaci (2.6%); from six birds showing severe prostration Pasteurella multocida was isolated. Virological and serological tests were negative for Avian Influenza virus (AIV), West Nile virus (WNV) and only three samples were positive for Newcastle disease virus (NDV) but only at serology (titre 1:16)

The sustainability of urban food systems : the case of mozzarella production in the city of Milan

Background: Urban food production in the developed world has recently been facing the challenge of feeding the world\u2019s cities adequately and on a sustainable basis, and innovative models of food production can help this transition. Nevertheless, this field of research largely focuses on primary food production\u2014so-called urban farming\u2014while the subsequent steps of the food chain have scarcely been investigated. In this context, this study aimed to provide a preliminary evaluation of urban food processing, taking mozzarella cheese production in the city of Milan as a case study. Methods: Five urban farmers participated in a direct survey aiming at identifying how sustainable this specific production chain can be considered. Results: Results demonstrated that the concept of urban food production is really valorized through measures able to enhance the global sustainability of the food production process only in some of the dairy micro- enterprises and that the economic dimension must be considered the variable most critically involved in hampering the global sustainability of this specific food chain. Conclusions: This research suggests the need to disseminate a global governance of urban supply chains and to support local activities, which represent a sensitive and strategic sector

Haemorrhagic enteritis seroconversion in turkey breeders: field observations

Seroconversion to viral haemorrhagic enteritis (HE) was studied in seven flocks of turkey breeders (17.974 birds in total), after 20 weeks of the onset of egg production. They showed no clinical signs, and mortality rate was normal. However, the infection caused a drop in egg production lasting about five weeks (-2.32 eggs laid during this period), but had no effect on hatching parameters

Predictive value of hematological and phenotypical parameters on postchemotherapy leukocyte recovery

Background: Grade IV chemotherapy toxicity is defined as absolute neutrophil count &lt;500/μL. The nadir is considered as the lowest neutrophil number following chemotherapy, and generally is not expected before the 7th day from the start of chemotherapy. The usual prophylactic dose of rHu-G-CSF (Filgrastim) is 300 μg/day, starting 24-48 h after chemotherapy until hematological recovery. However, individual patient response is largely variable, so that rHu-G-CSF doses can be different. The aim of this study was to verify if peripheral blood automated flow cytochemistry and flow cytometry analysis may be helpful in predicting the individual response and saving rHu-G-CSF. Methods: During Grade IV neutropenia, blood counts from 30 cancer patients were analyzed daily by ADVIA 120 automated flow cytochemistry analyzer and by Facscalibur flow cytometer till the nadir. "Large unstained cells" (LUCs), myeloperoxidase index (MPXI), blasts, and various cell subpopulations in the peripheral blood were studied. At nadir rHu-G-CSF was started and 81 chemotherapy cycles were analyzed. Cycles were stratified according to their number and to two dose-levels of rHuG-CSF needed to recovery (300-600 vs. 900-1200 μg) and analyzed in relation to mean values of MPXI and mean absolute number of LUCs in the nadir phase. The linear regressions of LUCs % over time in relation to two dose-levels of rHu-G-CSF and uni-multivariate analysis of lymphocyte subpopulations, CD34+ cells, MPXI, and blasts were also performed. Results: In the nadir phase, the increase of MPXI above the upper limit of normality (&gt;10; median 27.7), characterized a slow hematological recovery. MPXI levels were directly related to the cycle number and inversely related to the absolute number of LUCs and CD34 +/CD45+ cells. A faster hematological recovery was associated with a higher LUC increase per day (0.56% vs. 0.25%), higher blast (median 36.7/μL vs. 19.5/μL) and CD34+/CD45+ cell (median 2.2/μL vs. 0.82/μL) counts. Conclusions: Our study showed that some biological indicators such as MPXI, LUCs, blasts, and CD34 +/CD45+ cells may be of clinical relevance in predicting individual hematological response to rHu-G-CSF. Special attention should be paid when nadir MPXI exceeds the upper limit of normality because the hematological recovery may be delayed. © 2009 Clinical Cytometry Society

The sustainability of biodynamic horticultural production : The case of Po valley

More and more attention is paid to environmental, economic and social sustainability of the agricultural activity as it is proved at European level by the new setting of CAP toward 2020. It is therefore interesting to understand which production practices better react to these characteristics. Biodynamic appears to respond well in terms of environmental sustainability: can the same be affirmed in terms of economic sustainability? In this paper Italian biodynamic sector has been analyzed focusing on horticultural production: its economic sustainability has been verified especially for small farms that are facing increasing problems in terms of profitability. The Italian Demeter database was used to identify biodynamic farms that have been georeferenced at a national level and then only the ones specialized in vegetable production have been selected for Italy. In order to assess its economic sustainability the production costs for three farms located in the area of the High Po valley that are converting from organic to biodynamic have been examined, focusing on five items: lettuce (Lactuca sativa), tomato (Solanum lycopersicum), zucchini (Cucurbita pepo), eggplant (Solanum melongena), cucumber (Cucumis sativus). Then these costs have been compared to the ones associated with organic and traditional methods. Their prices have been also compared in order to achieve an economic assessment of the different production methods. Finally a SWOT analysis regarding the adoption of the biodynamic method has been performed. This paper proves that the biodynamic method is economically sustainable in the horticultural production based on higher retail prices that-for some products-compensate higher costs, in comparison to the organic and traditional methods. Another factor that strengthens the use of the biodynamic method is a favorable European legislation

When Innovation meets Tradition : the case of &quot; Riso &amp;Rane&quot; Rural District in Lombardy Region

Lombardy, with 87.393 hectares of rice is one of the leader region for this production in Italy and in the European Union (EU) too. This area is characterized by a strong connection with tradition both in terms of agricultural landscape and food culture. Nevertheless, during the last decade, farmers faced increasing competitiveness issues, mostly related to EU subsidies losses, market prices and the technical constraints of the traditional rice supply chain: provider of technical means, farmers, brokers and rice mill. In this scenario, the \u201cRiso e Rane\u201d Rural District (R&RD) supports farmers in improving competitiveness through innovation. The aim of the paper is to investigate the innovation in the rice supply chain related to the specific action of R&RD, that accounts for 60 farms. Starting from the direct survey carried out on the district productive structures, we investigate the farms' degree of innovation related to the adoption of a new model of supply chain. The case study areas is characterized by rice that represents the most important culture with 2.773 hectares (more than 58% of the district Utilized Agricultural Area (UAA)). In 2012, R&RD won a regional project titled \u201cBuono, Sano e Vicino\u201d with the aim to help local rice farmers developing an alternative supply chain in which the district grow into the local actor to increase farmers bargaining power and promotes new market strategies. To make this the attention was focused on one of the most important variety of Italian rice: Carnaroli. The main results of the study showed that the project was able to innovate the traditional supply chain in all the four innovation areas according to OECD (2005): product, process, market and organization. In conclusion, our results suggest that the R&RD is able to respond to farmers necessities in term of market competitiveness and to improve the sustainability of local food system