No evidence of association between NOD2/CARD15 gene polymorphism and atherosclerotic events after renal transplantation.

International audienceStable renal transplant recipients (RTR) display high rates of atherosclerotic events (AE). Innate immunity and especially vascular inflammation play a role in the pathogenesis of atherosclerosis. It is illustrated both by an increased occurrence of postrenal transplant cardiovascular events in patients with elevated levels of C-reactive protein and by a correlation between posttransplant AE and Toll-like receptor-4 Asp299Gly polymorphism. Here, we analyze the influence NOD2/CARD15 gene polymorphism since NOD2 can modulate macrophage pro-inflammatory activity and macrophage is present in early atherosclerotic lesions. The incidence of single nucleotide polymorphism (SNP) in the three major polymorphic region of NOD2 gene (SNP8, SNP12 and SNP13) was assessed in 182 RTR and the correlation between such polymorphism and the development of AE was analyzed. No correlation was observed between NOD2 gene polymorphism and the occurrence of AE after renal transplantation. NOD2 gene polymorphism thus does not appear to influence cardiovascular complications in RTR

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P < 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

Immunomodulation post allogreffe de moelle osseuse par l'approche gène suicide (analyse transcriptomique des lymphocytes T CD8+ produits ex vivo par voie rétrovirale et analyse cellulaire et moléculaire des cellules génétiquement modifiées après circulation in vivo chez des patients)

Nous avons réalisé un essai clinique de phase I/II en infusant des lymphocytes T génétiquement modifiés (CGM) exprimant le gène Herpes-Simplex thymidine kinase (HS-tk) lors d'une greffe de moelle. Ex-vivo, nous avons montré que notre méthode de production de CGM (CD3 activation/NéoR sélection) induit des anomalies phénotypiques et fonctionnelles, limitées en suivant un protocole expérimental (CD3-CD28 activation / NGFR-MACS sélection). ln vivo, parmi les 12 patients inclus, 4 sont en vie plus de 9 ans post-greffe. Les CGM circulantes NéoR+ sont > aux HS-tk+, suggérant des délétions du gène HS-tk. Cette étude a pour but : 1/ d'obtenir, dans différentes conditions de cultures, la signature transcriptomique des CGM destinées aux patients ; et 2/ d'identifier et caractériser les altérations du gène HS-tk. - Nos résultats montrent que : 1/ le protocole expérimental est moins délétère et pourrait être utilisé en immunothérapie ; et 2/ la délétion du gène HS-tk a lieu ex-vivo lors de la production des CGM.We performed a phase I/II clinical trial of Herpes-Simplex thymidine kinase (HS-tk) expressing gene-modified T cell (GMTC) infusion at time of bone marrow transplantation (BMT). Ex-vivo, we have previously shown that our GMTC production method (CD3 activation / NeoR selection) entails phenotypical and functional alterations, limited by an experimental protocol process (CD3-CD28 activation / NGFR-MACS selection). ln vivo, among 12 patients included, 4 are still alive more than 9 years after BMT. Circulating GMTC-NeoR+ is > to GMTC-HS-tk+, suggesting HS-tk transgene deletions. The present study aims : 1/ at providing a picture of the gene profile signature of GMTC, which may be infused into patients, in different conditions of production ; and 2/ at identifying and characterize the HS-tk alterations. - Our results revealed that : 1/ experimental protocol is less deleterious and should be used in immunotherapy ; and 2/ the whole HS-tk deletion occurs during the ex-vivo production process.BESANCON-BU Médecine pharmacie (250562102) / SudocSudocFranceF

Can an artificial cave be a hotspot for mollusc diversity? Overview of land snail biodiversity underneath Paris and surrounding area

International audienc

Can an artificial cave be a hotspot for mollusc diversity? Overview of land snail biodiversity underneath Paris and surrounding area

International audienc

A new species of Laoennea microsnail (Stylommatophora, Diapheridae) from a cave in Laos

The genus Laoennea (Streptaxoidea, Diapheridae) was erected by Páll-Gergely, 2020. The type species, Laoennea carychioides Páll-Gergely, A. Reischütz & Maassen, 2020 is so far only known from the type locality cave in Laos. Herein, we describe a second species, Laoennea renouardi sp. nov., from a nearby cave in the same karst region of Vientiane Province

A new species of Laoennea microsnail (Stylommatophora, Diapheridae) from a cave in Laos

The genus Laoennea (Streptaxoidea, Diapheridae) was erected by Páll-Gergely, 2020. The type species, Laoennea carychioides Páll-Gergely, A. Reischütz & Maassen, 2020 is so far only known from the type locality cave in Laos. Herein, we describe a second species, Laoennea renouardi sp. nov., from a nearby cave in the same karst region of Vientiane Province

A new species of Laoennea microsnail (Stylommatophora, Diapheridae) from a cave in Laos

The genus Laoennea (Streptaxoidea, Diapheridae) was erected by Páll-Gergely, 2020. The type species, Laoennea carychioides Páll-Gergely, A. Reischütz & Maassen, 2020 is so far only known from the type locality cave in Laos. Herein, we describe a second species, Laoennea renouardi sp. nov., from a nearby cave in the same karst region of Vientiane Province

Interaction between photoperiod and variation in circadian rhythms in tomato

12 Pág.Many biological processes follow circadian rhythmicity and are controlled by the circadian clock. Predictable environmental changes such as seasonal variation in photoperiod can modulate circadian rhythms, allowing organisms to adjust the timing of their biological processes to the time of the year. In some crops such as rice, barley or soybean, mutations in circadian clock genes have altered photoperiod sensitivity, enhancing their cultivability in specific seasons and latitudes. However, how changes in circadian rhythms interact with the perception of photoperiod in crops remain poorly studied. In tomato, the appearance during domestication of mutations in EMPFINDLICHER IM DUNKELROTEN LICHT 1 (EID1, Solyc09g075080) and NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED GENE 2 (LNK2, Solyc01g068560) delayed both the phase and period of its circadian rhythms. The fact that variation in period and phase are separated in tomato provides an optimal tool to study how these factors affect the perception of photoperiod.This research was funded by Saclay Plant Sciences grant SPS-2020-LIGHT. Funding for X.Y., P.R and T.S. was provided by ANR grants ANR-18-CE92-0039–01 and ANR-17-CE20-0024–02.Peer reviewe