Effect of the growth medium composition on nitrate accumulation in the novel protein crop Lemna minor

Duckweed is a potential alternative protein source for food and feed. However, little is known about the nitrate accumulation in this plant. A high nitrate level in vegetables can indirectly lead to an elevated intake of nitrites and N-nitroso compounds, increasing the risk of diseases for humans and animals. This research hypothesizes that the nitrate accumulation of Lemna minor differs between growing media. Additionally, it evaluates whether legal safety levels of nitrate for human and animal intake are exceeded. The duckweed was grown on (i) rainwater, and (ii) three synthetic media containing different nutrient levels. Furthermore, (iii) biological effluent of swine manure treatment and (iv) aquaculture effluent from pikeperch production were used, as these are potential media for closing nutrient loops in the agriculture sector. It was found that nitrate levels increased with the increasing availability of macronutrients in the water, and pH showed a particularly strong negative correlation with the nitrate levels in the plant. Nevertheless, nitrate content never exceeded 530 mg NO3 kg−1 fresh weight. To conclude, Lemna minor's nitrate content was below safety limits for human consumption in all tested growing media; however, a potential risk for ruminants was observed as these are more sensitive to nitrate conversions in their gastro-intestinal track

Lemna minor cultivation for treating swine manure and providing micronutrients for animal feed

The potential of Lemna minor to valorise agricultural wastewater into a protein-rich feed component to meet the growing demand for animal feed protein and reduce the excess of nutrients in certain European regions was investigated. Three pilot-scale systems were monitored for nine weeks under outdoor conditions in Flanders. The systems were fed with a mixture of the liquid fraction and the biological effluent of a swine manure treatment system diluted with rainwater in order that the weekly N and P addition was equal to the N and P removal by the system. The design tested the accumulation of elements in a continuous recirculation system. Potassium, Cl, S, Ca, and Mg were abundantly available in the swine manure wastewaters and tended to accumulate, being a possible cause of concern for long-operating recirculation systems. The harvested duckweed was characterised for its mineral composition and protein content. In animal husbandry, trace elements are specifically added to animal feed as micronutrients and, thus, feedstuffs biofortified with essential trace elements can provide added value. Duckweed grown on the tested mixture of swine manure waste streams could be considered as a source of Mn, Zn, and Fe for swine feed, while it is not a source of Cu for swine feed. Moreover, it was observed that As, Cd, and Pb content were below the limits of the feed Directive 2002/32/EC in the duckweed grown on the tested medium. Overall, these results demonstrate that duckweed can effectively remove nutrients from agriculture wastewaters in a recirculated system while producing a feed source with a protein content of 35% DM

Agronomic and environmental performance of Lemna minor cultivated on agricultural wastewater streams : a practical approach

This study investigated the potential of Lemna minor to valorise agricultural wastewater in protein-rich feed material in order to meet the growing demand for animal feed protein and reduce the excess of nutrients in certain European regions. For this purpose, three pilot-scale systems were monitored for 175 days under outdoor conditions in Flanders. The systems were fed with the effluent of aquaculture (pikeperch production—PP), a mixture of diluted pig manure wastewater (PM), and a synthetic medium (SM). PM showed the highest productivity (6.1 ± 2.5 g DW m−2 d−1) and N uptake (327 ± 107 mg N m−2 d−1). PP yielded a similar productivity and both wastewaters resulted in higher productivities than SM. Furthermore, all media showed similar P uptake rates (65–70 P m−2 d−1). Finally, duckweed had a beneficial amino acid composition for humans (essential amino acid index = 1.1), broilers and pigs. This study also showed that the growing medium had more influence on the productivity of duckweed than on its amino acid composition or protein content, with the latter being only slightly affected by the different media studied. Overall, these results demonstrate that duckweed can effectively remove nutrients from agriculture wastewaters while producing quality protein

Pathways to integrate Indigenous and local knowledge in ocean governance processes: Lessons from the Algoa Bay Project, South Africa

The Introduction of this paper argues that current coastal and ocean management approaches like marine spatial planning (MSP) often do not adequately acknowledge and integrate Indigenous and Local Knowledge (ILK). This is problematic because how humans value and perceive coastal and marine resources is integrally linked to how they use and manage these resources, especially in adapting to social-ecological change. Coastal and marine resources are situated within complex social-ecological systems that are culturally, economically, historically and politically embedded. Therefore, management approaches have to integrate transdisciplinary and contextual perspectives in order to be relevant, sustainable and adaptive. Following extensive research in Algoa Bay, South Africa this article highlights several pathways to bridge the gap between existing ILK and current coastal and ocean management approaches. The Methods section discusses how the authors worked in tandem with a bottom-up (engaging with Indigenous and local coastal and marine resource users) and top-down (engaging with coastal governance authorities and practitioners) approach. In order to answer the primary research question “How can ILK be integrated into area-based ocean management like MSP”? the authors employed arts-based participatory methods as well as in-depth interviews and workshops with coastal governance authorities and practitioners over several months. This work then culminated in a one-day multi-stakeholder workshop which brought both ILK holders and coastal authorities and practitioners together to collaboratively identify pathways to integrate this knowledge into coastal and ocean management. In the Results and Discussion section the authors present and discuss five co-identified pathways to integrate ILK in coastal and ocean management which include: adopting contextual approaches to coastal and ocean management; increasing transparency and two-way communication between coastal authorities and users; increasing access to relevant and useable information; reviewing and amending relevant MSP legislation towards a stronger connection between MSP and Indigenous knowledge legislation; as well as amending legislation pertaining to access to coastal and marine areas. In the Conclusion it is argued that ILK coastal communities want to be meaningfully included in how their coastline and ocean resources are managed and also seek increased access to coastal areas. By highlighting pathways to include ILK and the knowledge holders themselves, this paper seeks to contribute to improved protection and sustainable management of marine resource use

Pathways to integrate indigenous and local knowledge in ocean governance processes : lessons from the Algoa Bay Project, South Africa

The Introduction of this paper argues that current coastal and ocean management approaches like marine spatial planning (MSP) often do not adequately acknowledge and integrate Indigenous and Local Knowledge (ILK). This is problematic because how humans value and perceive coastal and marine resources is integrally linked to how they use and manage these resources, especially in adapting to social-ecological change. Coastal and marine resources are situated within complex social-ecological systems that are culturally, economically, historically and politically embedded. Therefore, management approaches have to integrate transdisciplinary and contextual perspectives in order to be relevant, sustainable and adaptive. Following extensive research in Algoa Bay, South Africa this article highlights several pathways to bridge the gap between existing ILK and current coastal and ocean management approaches. The Methods section discusses how the authors worked in tandem with a bottom-up (engaging with Indigenous and local coastal and marine resource users) and top-down (engaging with coastal governance authorities and practitioners) approach. In order to answer the primary research question “How can ILK be integrated into area-based ocean management like MSP”? the authors employed arts-based participatory methods as well as in-depth interviews and workshops with coastal governance authorities and practitioners over several months. This work then culminated in a one-day multi-stakeholder workshop which brought both ILK holders and coastal authorities and practitioners together to collaboratively identify pathways to integrate this knowledge into coastal and ocean management. In the Results and Discussion section the authors present and discuss five co-identified pathways to integrate ILK in coastal and ocean management which include: adopting contextual approaches to coastal and ocean management; increasing transparency and two-way communication between coastal authorities and users; increasing access to relevant and useable information; reviewing and amending relevant MSP legislation towards a stronger connection between MSP and Indigenous knowledge legislation; as well as amending legislation pertaining to access to coastal and marine areas. In the Conclusion it is argued that ILK coastal communities want to be meaningfully included in how their coastline and ocean resources are managed and also seek increased access to coastal areas. By highlighting pathways to include ILK and the knowledge holders themselves, this paper seeks to contribute to improved protection and sustainable management of marine resource use

BioTIME: A database of biodiversity time series for the Anthropocene.

MotivationThe BioTIME database contains raw data on species identities and abundances in ecological assemblages through time. These data enable users to calculate temporal trends in biodiversity within and amongst assemblages using a broad range of metrics. BioTIME is being developed as a community-led open-source database of biodiversity time series. Our goal is to accelerate and facilitate quantitative analysis of temporal patterns of biodiversity in the Anthropocene.Main types of variables includedThe database contains 8,777,413 species abundance records, from assemblages consistently sampled for a minimum of 2 years, which need not necessarily be consecutive. In addition, the database contains metadata relating to sampling methodology and contextual information about each record.Spatial location and grainBioTIME is a global database of 547,161 unique sampling locations spanning the marine, freshwater and terrestrial realms. Grain size varies across datasets from 0.0000000158 km2 (158 cm2) to 100 km2 (1,000,000,000,000 cm2).Time period and grainBioTIME records span from 1874 to 2016. The minimal temporal grain across all datasets in BioTIME is a year.Major taxa and level of measurementBioTIME includes data from 44,440 species across the plant and animal kingdoms, ranging from plants, plankton and terrestrial invertebrates to small and large vertebrates.Software format.csv and .SQL

Validation of the Body Concealment Scale for Scleroderma (BCSS): Replication in the Scleroderma Patient-centered Intervention Network (SPIN) Cohort

© 2016 Elsevier Ltd Body concealment is an important component of appearance distress for individuals with disfiguring conditions, including scleroderma. The objective was to replicate the validation study of the Body Concealment Scale for Scleroderma (BCSS) among 897 scleroderma patients. The factor structure of the BCSS was evaluated using confirmatory factor analysis and the Multiple-Indicator Multiple-Cause model examined differential item functioning of SWAP items for sex and age. Internal consistency reliability was assessed via Cronbach's alpha. Construct validity was assessed by comparing the BCSS with a measure of body image distress and measures of mental health and pain intensity. Results replicated the original validation study, where a bifactor model provided the best fit. The BCSS demonstrated strong internal consistency reliability and construct validity. Findings further support the BCSS as a valid measure of body concealment in scleroderma and provide new evidence that scores can be compared and combined across sexes and ages