The Effect of the Extra Virgin Olive Oil Minor Phenolic Compound 3′,4′-Dihydroxyphenylglycol in Experimental Diabetic Kidney Disease

The aim of this study was to analyze the possible nephroprotective effect of 3’,4’-dihydroxyphenylglycol (DHPG), a polyphenolic compound of extra virgin olive oil (EVOO), on renal lesions in an experimental model of type 1 diabetes. Rats were distributed as follows: healthy normoglycemic rats (NDR), diabetic rats treated with saline (DR), and DR treated with 0.5 mg/kg/day or 1 mg/kg/day of DHPG. DR showed a significantly higher serum and renal oxidative and nitrosative stress profile than NDR, as well as reduced prostacyclin production and renal damage (defined as urinary protein excretion, reduced creatinine clearance, increased glomerular volume, and increased glomerulosclerosis index). DHPG reduced the oxidative and nitrosative stress and increased prostacyclin production (a 59.2% reduction in DR and 34.7–7.8% reduction in DHPG-treated rats), as well as 38–56% reduction in urinary protein excretion and 22–46% reduction in glomerular morphological parameters (after the treatment with 0.5 or 1 mg/kg/day, respectively). Conclusions: DHPG administration to type 1-like diabetic rats exerts a nephroprotective effect probably due to the sum of its antioxidant (Pearson’s coefficient 0.68–0.74), antinitrosative (Pearson’s coefficient 0.83), and prostacyclin production regulator (Pearson’s coefficient 0.75) effects.This study was supported, in part, by the Consejería de Salud. Junta de Andalucía (Spain), Proyectos de Investigación en Salud [Regional Ministry of Health. Junta de Andalucía (Spain), Health Research Projects] (PI-0129-2017). Partial funding for open access charge: Universidad de Málag

Género y actividad física en mujeres mexicanas con experiencia migratoria a Estados Unidos de América

Objetivo Analizar la influencia de la categoría de género en la práctica de actividad física en mujeres de zonas rurales con experiencia migratoria a Estados Unidos de América.Métodos Diseño cualitativo con métodos de la teoría fundamentada; la información fue obtenida a través de entrevistas en profundidad a 19 mujeres que habitan en localidades rurales de la zona central de MéxicoResultados A través del análisis emergió como categoría central la crítica social a la actividad física. Las mujeres con pareja no realizan actividad física porque de acuerdo con las normas sociales es mal visto que la efectúen, y son los hombres quienes toman la decisión de permitirla. El género, la identidad femenina y la crítica social son elementos que contribuyen a la comprensión de la inactividad física en estas mujeres.Conclusión Es necesario que los programas sanitarios que promueven la actividad física dirigidos a mujeres adultas en zonas rurales, se diseñen tomando como base la perspectiva de género y el contexto de la población

CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells

The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that EWSR1::FLI1 levels can fluctuate in Ewing sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high cells are more proliferative. In this work, we described how the CD44 standard isoform (CD44s), a transmembrane protein involved in cell adhesion and migration, is overexpressed in the EWSR1::FLI1low phenotype. The functional characterization of CD44s (proliferation, clonogenicity, migration, and invasion ability) was performed in three doxycycline-inducible Ewing sarcoma cell models (A673, MHH-ES1, and CADO-ES1). As a result, CD44s expression reduced cell proliferation in all the cell lines tested without affecting clonogenicity. Additionally, CD44s increased cell migration in A673 and MHH-ES1, without effects in CADO-ES1. As hyaluronan is the main ligand of CD44s, its effect on migration ability was also assessed, showing that high molecular weight hyaluronic acid (HMW-HA) blocked cell migration while low molecular weight hyaluronic acid (LMW-HA) increased it. Invasion ability was correlated with CD44 expression in A673 and MHH-ES1 cell lines. CD44s, upregulated upon EWSR1::FLI1 knockdown, regulates cell migration and invasion in Ewing sarcoma cells.This project was funded by Instituto de Salud Carlos III, grant numbers PI20CIII/00020, DTS18CIII/00005, Asociación Pablo Ugarte, grant numbers TRPV205/18; Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, grant numbers TVP333-19, TVP-1324/15; ASION, grant number TVP141/17. Enrique Fernández-Tabanera is supported by Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, Saint T. Cervera is supported by Asociación Pablo Ugarte and Raquel M. Melero is supported by a CIBERER contract.S

Principios y métodos de la psicología del aprendizaje aplicados a ambientes educativos (Texto programado)

El texto que tiene en sus manos no pretende ser tanto una profundización en los principios de la Psicología del Aprendizaje que sirven de base a las técnicas de Modificación de Conducta, como una forma útil de estructurar unos contenidos básicos que constituyen una herramienta de trabajo válida para determinados colectivos (profesores de jardín de infancia o de E.G.B., padres, alumnos de magisterio o de primeros cursos de Psicología o Pedagogía, etc...) en su quehacer cotidiano. Puede ser aprovechado por el profesor que tiene que enfrentarse diariamente con alumnos que presentan frecuentes conductas disruptivas, con otros cuyo rendimiento escolar es deficitario o bien, con clases completas cuya composición y características dificulta el desarrollo adecuado de los objetivos académicos. Por otra parte, puede resultar también útil para padres que quieran conocer las razones que explican algunos comportamientos de sus hijos y los mecanismos que facilitan una modificación de aquellos más perjudiciales para su desarrollo psíquicamente sano. Por último, un tercer grupo destinatario de este libro serían los alumnos de magisterio y aquellos otros que se inician en las carreras de Psicología y Pedagogía, los cuales pueden encontrar en él un texto introductorio que les permita adquirir unos conocimientos básicos de los principios del condicionamiento y unas aplicaciones sencillas de los mismos al ámbito de las primeras etapas de la educación

Common Infectious Agents and Monoclonal B-cell Lymphocytosis: A Cross-sectional Epidemiological Study Among Healthy Adults

Background: Risk factors associated with monoclonal B-cell lymphocytosis (MBL), a potential precursor of chronic lymphocytic leukaemia (CLL), remain unknown. Methods: Using a cross-sectional study design, we investigated demographic, medical and behavioural risk factors associated with MBL. "Low-count" MBL (cases) were defined as individuals with very low median absolute count of clonal B-cells, identified from screening of healthy individuals and the remainder classified as controls. 452 individuals completed a questionnaire with their general practitioner, both blind to the MBL status of the subject. Odds ratios (OR) and 95% confidence interval (CI) for MBL were estimated by means of unconditional logistic regression adjusted for confounding factors. Results: MBL were detected in 72/452 subjects (16%). Increasing age was strongly associated with MBL (P-trend<0.001). MBL was significantly less common among individuals vaccinated against pneumococcal or influenza (OR 0.49, 95% confidence interval (CI): 0.25 to 0.95; P-value = 0.03 and OR: 0.52, 95% CI: 0.29 to 0.93, P-value = 0.03, respectively). Albeit based on small numbers, cases were more likely to report infectious diseases among their children, respiratory disease among their siblings and personal history of pneumonia and meningitis. No other distinguishing epidemiological features were identified except for family history of cancer and an inverse relationship with diabetes treatment. All associations described above were retained after restricting the analysis to CLL-like MBL. Conclusion: Overall, these findings suggest that exposure to infectious agents leading to serious clinical manifestations in the patient or its surroundings may trigger immune events leading to MBL. This exploratory study provides initial insights and directions for future research related to MBL, a potential precursor of chronic lymphocytic leukaemia. Further work is warranted to confirm these findings

Serum amyloid a1/toll-like receptor-4 Axis, an important link between inflammation and outcome of TBI patients

Traumatic brain injury (TBI) is one of the leading causes of mortality and disability world-wide without any validated biomarker or set of biomarkers to help the diagnosis and evaluation of the evolution/prognosis of TBI patients. To achieve this aim, a deeper knowledge of the biochemical and pathophysiological processes triggered after the trauma is essential. Here, we identified the serum amyloid A1 protein-Toll-like receptor 4 (SAA1-TLR4) axis as an important link between inflammation and the outcome of TBI patients. Using serum and mRNA from white blood cells (WBC) of TBI patients, we found a positive correlation between serum SAA1 levels and injury severity, as well as with the 6-month outcome of TBI patients. SAA1 levels also correlate with the presence of TLR4 mRNA in WBC. In vitro, we found that SAA1 contributes to inflammation via TLR4 activation that releases inflammatory cytokines, which in turn increases SAA1 levels, establishing a positive proinflammatory loop. In vivo, post-TBI treatment with the TLR4-antagonist TAK242 reduces SAA1 levels, improves neurobehavioral outcome, and prevents blood–brain barrier disruption. Our data support further evaluation of (i) post-TBI treatment in the presence of TLR4 inhibition for limiting TBI-induced damage and (ii) SAA1-TLR4 as a biomarker of injury progression in TBI patientsThis work was supported by grants from Fundación Mutua Madrileña and Fondo de Investigaciones Sanitarias (FIS) (ISCIII/FEDER) (Programa Miguel Servet CP14/00008; CPII19/00005; PI16/00735; PI19/00082) to JE, RYC2019-026870-I to JMR and PI18/01387 to A

Adaptación de las asignaturas básicas de primer curso de la ETSI Navales de la UPM: Actividades 2008-2009

En el marco de la reforma de las titulaciones con motivo del Espacio Europeo de Educación un grupo de profesores hemos coordinado, durante el curso 2008-2009, todas las asignaturas básicas de primer curso y una más de segundo curso en la Escuela Técnica Superior de Ingenieros Navales. Las actividades realizadas son: a) Coordinación de todas las asignaturas básicas de primer curso, con reuniones periódicas de coordinación horizontal y el establecimiento de una página web de moodle para profesores como espacio para el trabajo cooperativo. Particularmente importante es el establecimiento de un calendario conjunto de pruebas de evaluación continua. b) Redacción de guías de aprendizaje, con un formato común para todas las asignaturas, incluyendo los objetivos formativos, los contenidos, las actividades formativas, los enlaces y la bibliografía. c) Establecimiento de una plataforma de teleeducación común para todas las asignaturas, uno de los objetivos fundamentales del proyecto, ya que coexistían dos plataformas distintas. Igualmente importante ha sido reforzar los contenidos y las actividades que se podían realizar en la plataforma. d) Seguimiento del tiempo dedicado por los alumnos, hemos ido siguiendo el tiempo dedicado por los alumnos a las distintas asignaturas, para detectar si el tiempo que se dedica está en los márgenes establecidos en los créditos ECTS. Igualmente, hemos hecho dos encuestas a la mitad de cada semestre, para recoger las opiniones de los alumnos sobre las asignaturas y sobre los aspectos relevantes del proyecto. e) Organización de actividades de nivelación, para los alumnos de nuevo ingreso, con la organización de cursos cero y la participación y coordinación en la Plataforma de Punto de inicio de la UPM. f) Organización de actividades formativas, para poder llevar a cabo estas tareas, hemos organizado, en colaboración con el Centro y el Gabinete de Tele-Educación (GATE) actividades formativas relacionadas con la plataforma moodle, métodos de evaluación y de formación en competencias.En la presentación haremos una descripción de las actividades realizadas, así como una primera evaluación de las mismas. Por último, describiremos las tareas a desarrollar en los próximos cursos

LXR Nuclear receptors are transcriptional regulators of dendritic cell chemotaxis

The liver X receptors (LXRs) are ligand-activated nuclear receptors with established roles in the maintenance of lipid homeostasis in multiple tissues. LXRs exert additional biological functions as negative regulators of inflammation, particularly in macrophages. However, the transcriptional responses controlled by LXRs in other myeloid cells, such as dendritic cells (DCs), are still poorly understood. Here we used gain- and loss-of-function models to characterize the impact of LXR deficiency on DC activation programs. Our results identified an LXR-dependent pathway that is important for DC chemotaxis. LXR-deficient mature DCs are defective in stimulus-induced migration in vitro and in vivo. Mechanistically, we show that LXRs facilitate DC chemotactic signaling by regulating the expression of CD38, an ectoenzyme important for leukocyte trafficking. Pharmacological or genetic inactivation of CD38 activity abolished the LXR-dependent induction of DC chemotaxis. Using the low-density lipoprotein receptor-deficient (LDLR−/−) LDLR−/− mouse model of atherosclerosis, we also demonstrated that hematopoietic CD38 expression is important for the accumulation of lipid-laden myeloid cells in lesions, suggesting that CD38 is a key factor in leukocyte migration during atherogenesis. Collectively, our results demonstrate that LXRs are required for the efficient emigration of DCs in response to chemotactic signals during inflammation

Forest hydrology in Chile: Past, present, and future

This paper reviews the current knowledge of hydrological processes in Chilean temperate forests which extend along western South America from latitude 29° S to 56 ° S. This geographic region includes a diverse range of natural and planted forests and a broad sweep of vegetation, edaphic, topographic, geologic, and climatic settings which create a unique natural laboratory. Many local communities, endangered freshwater ecosystems, and downstream economic activities in Chile rely on water flows from forested catchments. This review aims to (i) provide a comprehensive overview of Chilean forest hydrology, to (ii) review prior research in forest hydrology in Chile, and to (iii) identify knowledge gaps and provide a vision for future research on forest hydrology in Chile. We reviewed the relation between native forests, commercial plantations, and other land uses on water yield and water quality from the plot to the catchment scale. Much of the global understanding of forests and their relationship with the water cycle is in line with the findings of the studies reviewed here. Streamflow from forested catchments increases after timber harvesting, native forests appear to use less water than plantations, and streams draining native forest yield less sediment than streams draining plantations or grassland/shrublands. We identified 20 key knowledge gaps such as forest groundwater systems, soil–plant-atmosphere interactions, native forest hydrology, and the effect of forest management and restoration on hydrology. Also, we found a paucity of research in the northern geographic areas and forest types (35-36 ° S); most forest hydrology studies in Chile (56 %) have been conducted in the southern area (Los Rios Region around 39-40 ° S). There is limited knowledge of the geology and soils in many forested areas and how surface and groundwater are affected by changes in land cover. There is an opportunity to advance our understanding using process-based investigations linking field studies and modeling. Through the establishment of a forest hydrology science “society” to coordinate efforts, regional and national-scale land use planning might be supported. Our review ends with a vision to advance a cross-scale collaborative effort to use new nation-wide catchment-scale networks Long-term Ecosystem Research (LTER) sites, to promote common and complementary techniques in these studies, and to conduct transdisciplinary research to advance sound and integrated planning of forest lands in Chile