20 research outputs found
Synthesis and characterization of cationic polymer nanoparticles and investigation of their interaction with seroalbumin
Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2018, Tutors: Carlos María Müller Jevenois, Gabriela Calderó LinnhoffToday, a big pharmaceutical research effort is dedicated to controlled and targeted release of drugs. One of the most innovative options is the use of nanoparticles as non-viral vectors for the controlled and/or targeted release of drugs towards target organs or tissues. In these fields, the use of polymer nanoparticles is widespread due to their versatility: they can be easily functionalized (polymers have reactive terminal groups such as carboxylic acids or amines) and therefore they are easily targeted to certain tissues or organs. Moreover, given the high (and modulable) biodegradability of certain polymers such as poly(lactic acid) (PLA) or poly(lactic-coglycolic) acid (PLGA) the rate of drug release can be easily controlled. This work focuses on the preparation and characterization of polymer nanoparticles made of ethylcellulose and PLGA from template nano-emulsions. By the incorporation of a cationic surfactant in the formulation, positive surface charges were attained. Positively charged nanoparticles allow for strong electrostatic interactions with anionic molecules such as proteins, which could be interesting for the design of targeted and controlled drug release systems.
Nano-emulsions were prepared by the PIC method using two slightly different techniques and were characterized by DLS (dynamic light scattering) and light transmittance versus time experiments.
Nanoparticles were prepared by solvent evaporation and were characterized by DLS and Zpotential
measurements. Their interaction with seroalbumin, the major protein in blood, was also studied through DLS and Z-potential measurements
Focal plasma cell osteomyelitis: an unusual presentation on the foot
Plasma cellular osteomyelitis is a chronic and recurrent non purulent bone inflammatory entity charactererized by
abundance of plasma cells in the lesional bone. Is a recurrent, unifocal or multifocal disorder, which has an
unknown etiology with clinical and radiographic features that may make it impossible to distinguish from other
non-suppurative sclerosing osteomyelitis. This rare condition usually occurs in metaphysis of a long bone of chil dren or young adults. We report a case of PCO in a 64-year-old woman with intermittent diffused pain in the hal lux of the left foot after several nail avulsions and two surgical procedures on the distal phalanx. The histological
study of the bone sample showed reactive sclerotic bone with plasma cells compatible with PCO. In spite of its
infectious origin, as in the present case the causal agent is not always identified. Owing to rarity of entity, a clini cal diagnosis is usually not considered and a histopathological examination is essential to establish of a definitive
diagnosis
A New Surgical Technique for the Treatment of Retronychia: Proximal Partial Nail Avulsion
[Resumen] La retroniquia u onicocriptosis proximal es una patología que se presenta con relativa frecuencia en las consultas de podología. La avulsión ungueal completa ha sido el tratamiento de elección descrito en la literatura para el abordaje de la patología. A pesar de ser efectiva, no está exenta de inconvenientes asociados a la pérdida de la función mecánica que la lámina ungueal tiene encomendada en el primer dedo de los pies. Para evitar o aminorar la aparición de estos inconvenientes, proponemos una alternativa sencilla consistente en la avulsión parcial proximal no incisional de la lámina ungueal dejando intacta la porción distal adherida al lecho hasta que la nueva lámina ungueal termine por desplazarla.[Abstract] Retronychia or proximal onychocryptosis is a relatively frequent reason for consultation in podiatrics offices. The complete nail avulsion has been the treatment of choice described in the literature for the approach of the pathology. Although this is an effective technic, it is not without drawbacks associated with the loss of the mechanical function that the nail plate has entrusted to the first toe. Toavoid or lessen the appearance of these problems, we propose a simple alternative consisting in partial non-incisional proximal avulsion of the nail plate leaving intact the distal nail portion adhered to the bed until it is displaced by the new nail plate
Red de apoyo local
Las redes sociales suelen estar constituidas por los miembros de la familia nuclear, amigos y amigas, vecinos y vecinas, compañeros y compañeras de trabajo y personas conocidas en la comunidad. Estos vínculos se convierten en sistemas de apoyo social, los cuales constituyen un elemento indispensable para la salud, ajuste y bienestar del individuo. El aspecto importante de las redes de apoyo es que son personas emocionalmente significativas para la persona en el caso que nos concierne y son quienes potencialmente brindan el apoyo social. Es decir estas redes sociales cumplen la función de proveer apoyo social, el cual genera herramientas necesarias para la persona dentro de su contexto cotidiano.
Teniendo en cuenta esto, las nuevas tecnologías nos brindan una buena herramienta para facilitar a personas con este deseo de buscar compañía de otra persona en situaciones de temor o ansiedad la posibilidad de establecer un proceso de comparación social, a través del cual se puede obtener información acerca de la situación estresante (lo que permite reducir la incertidumbre) y validar las propias reacciones (comparando sentimientos y conductas).
En esta memoria, detallaremos las herramientas que se van a poner a disposición de las usuarias de nuestra red de apoyo local
FOSL1 promotes cholangiocarcinoma via transcriptional effectors that could be therapeutically targeted
[EN] Background & Aims: Cholangiocarcinoma (CCA) is a neoplasia of
the biliary tract driven by genetic, epigenetic and transcriptional
mechanisms. Herein, we investigated the role of the transcription
factor FOSL1, as well as its downstream transcriptional effectors,
in the development and progression of CCA.
Methods: FOSL1 was investigated in human CCA clinical samples.
Genetic inhibition of FOSL1 in human and mouse CCA cell
lines was performed in in vitro and in vivo models using
constitutive and inducible short-hairpin RNAs. Conditional
FOSL1 ablation was done using a genetically engineered mouse
(GEM) model of CCA (mutant KRAS and Trp53 knockout). Followup
RNA and chromatin immunoprecipitation (ChIP) sequencing
analyses were carried out and downstream targets were validated
using genetic and pharmacological inhibition.
Results: An inter-species analysis of FOSL1 in CCA was conducted.
First, FOSL1 was found to be highly upregulated in human
and mouse CCA, and associated with poor patient survival.
Pharmacological inhibition of different signalling pathways in
CCA cells converged on the regulation of FOSL1 expression.
Functional experiments showed that FOSL1 is required for cell
proliferation and cell cycle progression in vitro, and for tumour
growth and tumour maintenance in both orthotopic and subcutaneous
xenograft models. Likewise, FOSL1 genetic abrogation
in a GEM model of CCA extended mouse survival by decreasing
the oncogenic potential of transformed cholangiocytes. RNA and
ChIP sequencing studies identified direct and indirect transcriptional
effectors such as HMGCS1 and AURKA, whose genetic
and pharmacological inhibition phenocopied FOSL1 loss.
Conclusions: Our data illustrate the functional and clinical
relevance of FOSL1 in CCA and unveil potential targets amenable
to pharmacological inhibition that could enable the implementation
of novel therapeutic strategies.
Lay summary: Understanding the molecular mechanisms
involved in cholangiocarcinoma (bile duct cancer) development
and progression stands as a critical step for the development of
novel therapies. Through an inter-species approach, this study
provides evidence of the clinical and functional role of the
transcription factor FOSL1 in cholangiocarcinoma. Moreover, we
report that downstream effectors of FOSL1 are susceptible to
pharmacological inhibition, thus providing new opportunities
for therapeutic intervention.A.V. was supported by ADA of the University of Navarra, Spain,
O.E. by FSE; MINECO; FJCI-2017-34233, Spain, R.E. by a donation
from Mauge Burgos de la Iglesia’s family, Spain, and P. Olaizola by
the Basque Government (PRE_2016_1_0269), Basque Country,
Spain. M.J.P. was funded by ISCIII [FIS PI14; 00399, PI17; 00022]
cofinanced by “Fondo Europeo de Desarrollo Regional” (FEDER),
Spain; Spanish Ministry of Economy and Competitiveness
(MINECO: “Ramón y Cajal” Program RYC-2015-17755), Spain.
M.A.A was funded by La Caixa Foundation, HEPACARE project,
Spain, ISCIII FIS PI16/01126 cofinanced by “Fondo Europeo de
Desarrollo Regional” (FEDER), Spain, and “Fundación Científica de
la Asociación Española Contra el Cáncer’’ (AECC Scientific Foundation)
Rare Cancers 2017, Spain. J.M.B. was funded by the
Spanish Carlos III Health Institute (ISCIII) (FIS PI15; 01132, PI18;
01075 and Miguel Servet Program CON14; 00129 and CPII19;
00008), Spain, co-financed by “Fondo Europeo de Desarrollo
Regional” (FEDER), Spain; “Euskadi RIS3” (2019222054) and
BIOEF (Basque Foundation for Innovation and Health Research:
EiTB Maratoia BIO15; CA; 016; BD), Basque Country, Spain;
“Fundación Científica de la Asociación Española Contra el Cáncer”
(AECC Scientific Foundation) Rare Cancers 2017, Spain. S.V. was
supported by FEDER; MINECO (SAF2017-89944-R), Spain, by the
Government of Navarra-Health Research Department (58; 2018),
Navarra, Spain, by La Caixa and Caja Navarra Foundation-CIMA
agreement, Spain. None of the funding sources were involved
in the decision to submit the article for publication. This article is
based upon work from COST Action CA18122 European Cholangiocarcinoma
Network, supported by COST (European Cooperation
in Science and Technology). COST (European Cooperation in Science and Technology) is a funding agency for research and
innovation networks (www.cost.eu)
Turnip yellow mosaic virus in Chinese cabbage in Spain: Commercial seed transmission and molecular characterization
[EN] Seed transmission of Turnip yellow mosaic virus (TYMV, genus Tymovirus) was evaluated in the whole seeds and seedlings that emerged from three commercial Chinese cabbage (Brassica pekinensis) seed batches. Seedlings in the cotyledon stage and adult plants were assayed for TYMV by DAS-ELISA and confirmed by RT-PCR. The proportion of whole seeds infected with TYMV was at least 0.15 %. The seeds of the three seed batches were grown in Petri dishes, and surveyed in the cotyledon stage in trays that contained a peat:sand mixture grown in greenhouses or growth chambers, which were analysed in the cotyledon and adult stages. The seed-to-seedling transmission rate ranged from 2.5 % to 2.9 % in two different seed batches (lot-08 and lot-09, respectively). Spanish isolates derived from turnip (Sp-03) and Chinese cabbage (Sp-09 and Sp-13), collected in 2003, 2009 and 2013 in two different Spanish regions, were molecularly characterised by analysing the partial nucleotide sequences of three TYMV genome regions: partial RNA-dependent RNA polymerase (RdRp), methyltransferase (MTR) and coat protein (CP) genes. Phylogenetic analyses showed that the CP gene represented two different groups: TYMV-1 and TYMV-2. The first was subdivided into three subclades: European, Australian and Japanese. Spanish isolate Sp-03 clustered together with European TYMV group, whereas Sp-09 and Sp-13 grouped with the Japanese TYMV group, and all differed from group TYMV-2. The sequences of the three different genomic regions examined clustered into the same groups. The results suggested that Spanish isolates grouped according to the original hosts from which they were isolated. 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The use of a multiple-transfer method in plant virus transmission studies: some statistical points arising in the analysis of results. Annals of Applied Biology, 48, 75–83.Hayden, C. M., Mackenzie, A. M., & Gibbs, A. J. (1998a). Virion protein sequence variation among Australian isolates of turnip yellow mosaic tymovirus. Archives of Virology, 143, 191–201.Hayden, C. M., Mackenzie, A. M., Skotnicki, M. L., & Gibbs, A. (1998b). Turnip yellow mosaic virus isolates with experimentally produced recombinant virion proteins. Journal of General Virology, 79, 395–403.Hein, A. (1984). Transmission of Turnip yellow mosaic virus through seed of Camelina sativa gold of pleasure. Journal of Plant Diseases and Protection, 91, 549–551.Herrera-Vásquez, J. A., Córdoba-Sellés, M. C., Cebrián, M. C., Alfaro-Fernández, A., & Jordá, C. (2009). Seed transmission of Melon necrotic spot virus and efficacy of seed-disinfection treatments. Plant Pathology, 58, 436–452.Hull, R. (2002). Matthews’ plant virology (4a ed.1001 pp). San Diego: Academic Press.Johansen, E., Edwards, M. C., & Hampton, R. O. (1994). Seed transmission of viruses: current perspectives. Annual Review of Phytopathology, 32, 363–386.Kirino, N., Inoue, K., Tanina, K., Yamazaki, Y., & Ohki, S. T. (2008). Turnip yellow mosaic virus isolated from Chinese cabbage in Japan. Journal of General Plant Pathology, 74, 331–334.Markham, R., & Smith, K. S. (1949). Studies on the virus of turnip yellow mosaic. Parasitology, 39, 330–342.Mathews, R. E. F. (1980). Turnip yellow mosaic virus, CMI/AAB Descriptions of plant virus No. 230 (No. 2 revised). Kew: Commonwealth Mycology Institute/Association of Applied Biologists.Mitchell, E. J., & Bond, J. M. (2005). Variation in the coat protein sequence of British isolates of Turnip yellow mosaic virus and comparison with previously published isolates. Archives of Virology, 150, 2347–2355.Pagán, I., Fraile, A., Fernández-Fueyo, E., Montes, N., Alonso-Blanco, C., & García-Arenal, F. (2010). Arabidopsis thaliana as a model for the study of plant-virus co-evolution. Philosophical Transations of the Royal Society Biological Sciences, 365, 1983–1995.Paul, H. L., Gibbs, A., & Wittman-Liebold, B. (1980). The relationships of certain Tymoviruses assessed from the amino acid composition of their coat proteins. Intervirology, 13, 99–109.Pelikanova, J. (1990). Garlic mustard a spontaneous host of TYMV. Ochrana Rostlin, 26, 17–22.Procházková, Z. (1980). Host range and symptom differences between isolates of Turnip mosaic virus obtained from Sisymbrium loeselii. Biologia Plantarum, 22, 341–347.Rimmer, S. R., Shtattuck, V. I., & Buchwaldt, L. (2007). Compendium of brassica diseases (1ª Edición ed.p. 117). USA: APS press.Rot, M. E., & Jelkman, W. (2001). Characterization and detection of several filamentous viruses of cherry: Adaptation of an alternative cloning method (DOP-PCR), and modification of an RNA extraction protocol. European Journal of Plant Pathology, 107, 411–420.Sabanadzovic, S., Abou-Ghanem, N., Castellano, M. A., Digiaero, M., & Martelli, G. P. (2000). Grapevine fleck virus-like in Vitis. Archives of Virology, 145, 553–565.Špack, J., & Kubelková, D. (2000). Serological variability among European isolates of Radish mosaic virus. Plant Pathology, 49, 295–301.Špack, J., Kubelková, D., & Hnilicka, E. (1993). Seed transmission of Turnip yellow mosaic virus in winter turnip and winter oilseed rapes. Annals of Applied Biology, 123, 33–35.Stobbs, L. W., Cerkauskas, R. F., Lowery, T., & VanDriel, L. (1998). Occurrence of Turnip yellow mosaic virus on oriental cruciferours vegetables in Southern Ontario, Canada. Plant Disease, 82, 351.Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). 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Hacia la Sociedad de la Información y el Conocimiento: Informe 2012
Por séptimo año consecutivo el Prosic presenta su informe anual “Hacia la Sociedad de la Información y el Conocimiento en Costa Rica, 2012”, cuyo propósito es aportar cada año nueva información sobre cómo progresan las tecnologías de la información y comunicación en los diferentes sectores de la sociedad.
La Web es hoy en día la mayor fuente de información; definitivamente la forma de comunicarnos ha cambiado: la inmediatez del correo electrónico, los SMS, las redes sociales, el microblogging, los datos abiertos entre muchos otros, abren un gran abanico de oportunidades en la interactividad del quehacer diario: trabajo, estudios, investigación, diversión, familia etc. En esta era de la información las nuevas tecnologías están marcando un cambio fundamental en nuestro país; para muchos habitantes de la ciudad, es casi imposible imaginar la vida sin las TIC.
La importancia de este Informe anual del Prosic reside, entre otros elementos, en la exploración de dimensiones diversas concernientes a la incorporación de las tecnologías de información y comunicación en la interacción social cotidiana, tanto en el ámbito privado como público, en la educación, en la salud, en la ciencia y la investigación, la ingeniería, la física y en la economía; en fin en todos los sectores de esta sociedad de la información cada vez en mayor convergencia.UCR::Rectoría::Programa Sociedad de la Información y el Conocimiento (PROSIC
Natural History of MYH7-Related Dilated Cardiomyopathy
BACKGROUND Variants in myosin heavy chain 7 (MYH7) are responsible for disease in 1% to 5% of patients with dilated cardiomyopathy (DCM); however, the clinical characteristics and natural history of MYH7-related DCM are poorly described. OBJECTIVES We sought to determine the phenotype and prognosis of MYH7-related DCM. We also evaluated the influence of variant location on phenotypic expression. METHODS We studied clinical data from 147 individuals with DCM-causing MYH7 variants (47.6% female; 35.6 +/- 19.2 years) recruited from 29 international centers. RESULTS At initial evaluation, 106 (72.1%) patients had DCM (left ventricular ejection fraction: 34.5% +/- 11.7%). Median follow-up was 4.5 years (IQR: 1.7-8.0 years), and 23.7% of carriers who were initially phenotype-negative developed DCM. Phenotypic expression by 40 and 60 years was 46% and 88%, respectively, with 18 patients (16%) first diagnosed at <18 years of age. Thirty-six percent of patients with DCM met imaging criteria for LV noncompaction. During follow-up, 28% showed left ventricular reverse remodeling. Incidence of adverse cardiac events among patients with DCM at 5 years was 11.6%, with 5 (4.6%) deaths caused by end-stage heart failure (ESHF) and 5 patients (4.6%) requiring heart transplantation. The major ventricular arrhythmia rate was low (1.0% and 2.1% at 5 years in patients with DCM and in those with LVEF of <= 35%, respectively). ESHF and major ventricular arrhythmia were significantly lower compared with LMNA-related DCM and similar to DCM caused by TTN truncating variants. CONCLUSIONS MYH7-related DCM is characterized by early age of onset, high phenotypic expression, low left ventricular reverse remodeling, and frequent progression to ESHF. Heart failure complications predominate over ventricular arrhythmias, which are rare. (C) 2022 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation
Métodos y técnicas de monitoreo y predicción temprana en los escenarios de riesgos socionaturales
Esta obra concentra los métodos y las técnicas fundamentales para el seguimiento y monitoreo de las dinámicas de los escenarios de riesgos socionaturales (geológicos e hidrometeorológicos) y tiene como objetivo general orientar, apoyar y acompañar a los directivos y operativos de protección civil en aterrizar las acciones y políticas públicas enfocadas a la gestión del riesgo local de desastre
4to. Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad. Memoria académica
Este volumen acoge la memoria académica de la Cuarta edición del Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad, CITIS 2017, desarrollado entre el 29 de noviembre y el 1 de diciembre de 2017 y organizado por la Universidad Politécnica Salesiana (UPS) en su sede de Guayaquil.
El Congreso ofreció un espacio para la presentación, difusión e intercambio de importantes investigaciones nacionales e internacionales ante la comunidad universitaria que se dio cita en el encuentro. El uso de herramientas tecnológicas para la gestión de los trabajos de investigación como la plataforma Open Conference Systems y la web de presentación del Congreso http://citis.blog.ups.edu.ec/, hicieron de CITIS 2017 un verdadero referente entre los congresos que se desarrollaron en el país.
La preocupación de nuestra Universidad, de presentar espacios que ayuden a generar nuevos y mejores cambios en la dimensión humana y social de nuestro entorno, hace que se persiga en cada edición del evento la presentación de trabajos con calidad creciente en cuanto a su producción científica.
Quienes estuvimos al frente de la organización, dejamos plasmado en estas memorias académicas el intenso y prolífico trabajo de los días de realización del Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad al alcance de todos y todas