ANTONIO [Material gráfico]

Copia digital. Madrid : Ministerio de Educación, Cultura y Deporte. Subdirección General de Coordinación Bibliotecaria, 201

Determinants of the access to remote specialised services provided by national sarcoma reference centres

BACKGROUND: Spatial inequalities in cancer management have been evidenced by studies reporting lower quality of care or/and lower survival for patients living in remote or socially deprived areas. NETSARC+ is a national reference network implemented to improve the outcome of sarcoma patients in France since 2010, providing remote access to specialized diagnosis and Multidisciplinary Tumour Board (MTB). The IGéAS research program aims to assess the potential of this innovative organization, with remote management of cancers including rare tumours, to go through geographical barriers usually impeding the optimal management of cancer patients. METHODS: Using the nationwide NETSARC+ databases, the individual, clinical and geographical determinants of the access to sarcoma-specialized diagnosis and MTB were analysed. The IGéAS cohort (n = 20,590) includes all patients living in France with first sarcoma diagnosis between 2011 and 2014. Early access was defined as specialised review performed before 30 days of sampling and as first sarcoma MTB discussion performed before the first surgery. RESULTS: Some clinical populations are at highest risk of initial management without access to sarcoma specialized services, such as patients with non-GIST visceral sarcoma for diagnosis [OR 1.96, 95% CI 1.78 to 2.15] and MTB discussion [OR 3.56, 95% CI 3.16 to 4.01]. Social deprivation of the municipality is not associated with early access on NETSARC+ remote services. The quintile of patients furthest away from reference centres have lower chances of early access to specialized diagnosis [OR 1.18, 95% CI 1.06 to 1.31] and MTB discussion [OR 1.24, 95% CI 1.10 to 1.40] but this influence of the distance is slight in comparison with clinical factors and previous studies on the access to cancer-specialized facilities. CONCLUSIONS: In the context of national organization driven by reference network, distance to reference centres slightly alters the early access to sarcoma specialized services and social deprivation has no impact on it. The reference networks' organization, designed to improve the access to specialized services and the quality of cancer management, can be considered as an interesting device to reduce social and spatial inequalities in cancer management. The potential of this organization must be confirmed by further studies, including survival analysis

Détoxification d'hydolysats hémicellulosiques par dia-nanofiltration, étude de leur fermentescibilité et du mode d'action des inhibiteurs.

Lignocellulosic biomass is a promising alternative to oil for the production of energy and high added value molecules. However, its exploitation requires the use of pretreatment processes. The most commonly used processes generate by-products which present inhibitory action on microorganisms (furan derivatives, aliphatic acids, phenolic compounds).The main goal of this thesis project is to answer the following question : “Is it possible to use nanofiltration to remove inhibitory compounds from hemicellulosic hydrolysate, in order to ferment the latter with Bacillus subtilis 168 ?”Determination of minimal inhibitory concentrations of these inhibitory compounds allowed to determine, for each one, the quantity to remove from the hydrolysate.Dia-nanofiltration with DK membrane (GE, USA) allowed to efficiently remove (more than 90%) furane derivatives of aliphatic acids. However, removal of phenolic compounds was more complexe, hence, only 25% of syringaldehyde could be removed. Though most indentified inhibitory compounds were removed, Bacillus subtilis 168 was still not able to ferment the hydrolysate.Flow cytometry showed that férulic acid and vanillin presented an impact on Bacillus subtilis 168 plasma membrane fluidity of polarisation.La biomasse lignocellulosique (BLC) est, à l’heure actuelle, la meilleure alternative au pétrole en ce qui concerne la production d’énergie et de molécules à haute valeur ajoutée. Seulement, son exploitation nécessite l’emploi de prétraitements dont les plus courants génèrent des composés inhibant la croissance des microorganismes (dérivés furaniques, acides aliphatiques, phenols).Le but de ces travaux de thèse est donc de répondre à la question suivante : “Est il possible d’utiliser la nanofiltration pour éliminer les composés inhibiteurs présents dans un hydrolysat hémicellulosique, afin de le rendre fermentescible par Bacillus subtilis 168 ?”La détermination des concentrations minimales inhibitrices de ces composés inhibiteurs sur Bacillus subtilis 168 ont permis de fixer la quantité d’inhibiteurs à éliminer de l’hydrolysat.La dia-nanofiltration à l’aide de la membrane DK (GE, USA) a permis d’éliminer de manière efficace (+ de 90%) les dérivés furaniques et les acides alphatiques. Toutefois l’émination des composés phénoliques s’est révélée plus complexe, ainsi seul 25% du syringaldéhyde a été éliminé.La cytométrie en flux a permis de montrer une action de l’acide férulique et de la vanilline sur la fluidité et la polarisation de la membrane plasmique

Detoxification of hemicellulosic hydrolysates through dia-nanofiltration, study of their fermentescibility and mode of action of inhibitory compounds.

La biomasse lignocellulosique (BLC) est, à l’heure actuelle, la meilleure alternative au pétrole en ce qui concerne la production d’énergie et de molécules à haute valeur ajoutée. Seulement, son exploitation nécessite l’emploi de prétraitements dont les plus courants génèrent des composés inhibant la croissance des microorganismes (dérivés furaniques, acides aliphatiques, phenols).Le but de ces travaux de thèse est donc de répondre à la question suivante : “Est il possible d’utiliser la nanofiltration pour éliminer les composés inhibiteurs présents dans un hydrolysat hémicellulosique, afin de le rendre fermentescible par Bacillus subtilis 168 ?”La détermination des concentrations minimales inhibitrices de ces composés inhibiteurs sur Bacillus subtilis 168 ont permis de fixer la quantité d’inhibiteurs à éliminer de l’hydrolysat.La dia-nanofiltration à l’aide de la membrane DK (GE, USA) a permis d’éliminer de manière efficace (+ de 90%) les dérivés furaniques et les acides alphatiques. Toutefois l’émination des composés phénoliques s’est révélée plus complexe, ainsi seul 25% du syringaldéhyde a été éliminé.La cytométrie en flux a permis de montrer une action de l’acide férulique et de la vanilline sur la fluidité et la polarisation de la membrane plasmique.Lignocellulosic biomass is a promising alternative to oil for the production of energy and high added value molecules. However, its exploitation requires the use of pretreatment processes. The most commonly used processes generate by-products which present inhibitory action on microorganisms (furan derivatives, aliphatic acids, phenolic compounds).The main goal of this thesis project is to answer the following question : “Is it possible to use nanofiltration to remove inhibitory compounds from hemicellulosic hydrolysate, in order to ferment the latter with Bacillus subtilis 168 ?”Determination of minimal inhibitory concentrations of these inhibitory compounds allowed to determine, for each one, the quantity to remove from the hydrolysate.Dia-nanofiltration with DK membrane (GE, USA) allowed to efficiently remove (more than 90%) furane derivatives of aliphatic acids. However, removal of phenolic compounds was more complexe, hence, only 25% of syringaldehyde could be removed. Though most indentified inhibitory compounds were removed, Bacillus subtilis 168 was still not able to ferment the hydrolysate.Flow cytometry showed that férulic acid and vanillin presented an impact on Bacillus subtilis 168 plasma membrane fluidity of polarisation

PUD - « EXPLOITER LES DONNÉES QUANTITATIVES EN SHS »

La plateforme Universitaire de Données de Clermont-Ferrand a été créé le 1er septembre 2021. Elle s’adresse aux enseignants-chercheurs, chercheurs, doctorants ou encore aux étudiants en sciences humaines et sociales. L’objectif de la plateforme est d’aider les chercheurs à développer leurs compétences lors de la recherche et du traitement de données quantitatives.Axel DELARUE présente la Plateforme Universitaire de Données de la Maison des Sciences de l'Homme de Clermont-Ferrand

Flow cytometry: a relevant tool to assess the integrity, polarization, and fluidity of bacterial cytoplasmic membrane

Flow cytometry: a relevant tool to assess the integrity, polarization, and fluidity of bacterial cytoplasmic membrane. 33. Congress of the International Society for Advancement of Cytometry (CYTO 2018

Flow cytometry: a relevant tool to assess the integrity, polarization, and fluidity of bacterial cytoplasmic membrane

Flow cytometry: a relevant tool to assess the integrity, polarization, and fluidity of bacterial cytoplasmic membrane. 33. Congress of the International Society for Advancement of Cytometry (CYTO 2018

Membrane-based detoxification of lignocellulosic hydrolysates for production of a sugar enriched fermentation medium

Membrane-based detoxification of lignocellulosic hydrolysates for production of a sugar enriched fermentation medium. 12. International Conference on Renewable Resources and Biorefineries (RRB-12

Membrane-based detoxification of lignocellulosic hydrolysates for production of a sugar enriched fermentation medium

Membrane-based detoxification of lignocellulosic hydrolysates for production of a sugar enriched fermentation medium. 12. International Conference on Renewable Resources and Biorefineries (RRB-12