A Meta-Narrative Review of RUNX1-RUNX1T1 and its Relativity to CBF Disruption Within the Adult Population

Acute Myeloid Leukemia (AML) is an aggressive subgroup of leukemias developed from a deviant hematopoietic stem cell, prevented from differentiating into a mature cell. Additionally, core binding factor (CBF) is disrupted by the translocation of chromosomes 8 and 21. This balanced translocation generates the RUNX1-RUNX1T1 fusion gene on the derivative chromosome 8, resulting in t(8;21)(q22;q22.1). These events cause a blockage of hematopoietic stem cell differentiation and ultimately lead to leukemia transformation (Beghini, A. 2019). Using Fluorescence in situ hybridization (FISH) and Karyotyping, molecular translocation can be detected, visualized, and associated with chromosomes 8 and 21 [t(8;21)(q22;q22)]. Furthermore, this study will investigate how gene fusion and translocation disrupt CBF for the purpose of understanding how AML is diagnosed and treatment. To address this, we conducted a meta-narrative review of the number of articles that correspond to each sub-theme of the approach to AML in the context of RUNX1-RUNX1T1 fusion gene, including the utilization of treatments, its detail on correlation to CBF complex, how the research method was depicted, limitations, audience, technology, and treatment. One of the most important aspects of the study was the understanding of genetic abnormalities and the use of genetic analysis in developing future treatment strategies for leukemia. The correlation of RUNX1-RUNX1T1 gene generated on the derivative chromosome 8 will result in t(8;21)(q22;q22.1), a balanced translocation.https://openworks.mdanderson.org/rmps/1006/thumbnail.jp

Tamaño del nervio óptico detectado por tomografía coherencia óptica en pacientes sanos atendidos en un centro oftalmológico del Paraguay

El objetivo de este trabajo fue determinar el tamaño del disco óptico por tomografía de coherencia óptica (TCO), en una población mayor de 40 años, que asistió a control rutinario oftalmológico desde noviembre el 2015 a febrero del 2016 y que no tenían antecedentes conocidos de glaucoma ni de enfermedades sistémicas. Fueron incluidos en el estudio 52 pacientes que asistieron a la consulta externa de oftalmología de la Fundación Visión, por un examen de rutina. Se le diligenció una historia clínica completa, donde se indagaron los antecedentes patológicos tanto sistémicos como oculares. Se realizó la toma de la agudeza visual utilizando la cartilla de Snellen en cada ojo por separado a 6 metros del paciente, refracción automatizada, prueba refractiva, biomicroscopía en lámpara de hendidura con énfasis en la profundidad de la cámara anterior. Se excluyeron pacientes con cámaras anteriores pandas o estrechas (utilización de gonioscopio mirrow 4 mini) y presión intraocular elevada. Luego de la instilación de Tropicamida 0,5%/Fenilefrina HCL 5% en cada ojo y evaluación del polo posterior en lámpara de hendidura con lente de 90D Superfield, se realizó la Tomografía de coherencia óptica con el equipo The ZEISS Cirrus™ HD-TCO Model 4000 (Cirrus HD-TCO)

Transcriptome and proteome mapping in the sheep atria reveal molecular featurets of atrial fibrillation progression.

Atrial fibrillation (AF) is a progressive cardiac arrhythmia that increases the risk of hospitalization and adverse cardiovascular events. There is a clear demand for more inclusive and large-scale approaches to understand the molecular drivers responsible for AF, as well as the fundamental mechanisms governing the transition from paroxysmal to persistent and permanent forms. In this study, we aimed to create a molecular map of AF and find the distinct molecular programmes underlying cell type-specific atrial remodelling and AF progression. We used a sheep model of long-standing, tachypacing-induced AF, sampled right and left atrial tissue, and isolated cardiomyocytes (CMs) from control, intermediate (transition), and late time points during AF progression, and performed transcriptomic and proteome profiling. We have merged all these layers of information into a meaningful three-component space in which we explored the genes and proteins detected and their common patterns of expression. Our data-driven analysis points at extracellular matrix remodelling, inflammation, ion channel, myofibril structure, mitochondrial complexes, chromatin remodelling, and genes related to neural function, as well as critical regulators of cell proliferation as hallmarks of AF progression. Most important, we prove that these changes occur at early transitional stages of the disease, but not at later stages, and that the left atrium undergoes significantly more profound changes than the right atrium in its expression programme. The pattern of dynamic changes in gene and protein expression replicate the electrical and structural remodelling demonstrated previously in the sheep and in humans, and uncover novel mechanisms potentially relevant for disease treatment. Transcriptomic and proteomic analysis of AF progression in a large animal model shows that significant changes occur at early stages, and that among others involve previously undescribed increase in mitochondria, changes to the chromatin of atrial CMs, and genes related to neural function and cell proliferation.This work was supported by the Spanish government (BFU2017-84914-P to M.M.; FPI Fellowship to A.A.-F.; FPU Fellowship to R.R.), and in part by grants to J.J. from the National Heart, Lung and Blood Institute (R01 grant HL122352 NIH/NHLBI), the Leducq Foundation (Transatlantic Network of Excellence Program on Structural Alterations in the Myocardium and the Substrate for Cardiac Fibrillation), and the University of Michigan Health System–Peking University Health Science Center Joint Institute for Translational and Clinical Research (UMHS-PUHSC; project: Molecular Mechanisms of Fibrosis and the Progression from Paroxysmal to Persistent Atrial Fibrillation). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505).S

El estado nutricional y su impacto en los logros de aprendizaje

Se realizó un trabajo de investigación para identificar el Estado Nutricional en los niños y niñas de la Institución Educativa Inicial Pública “Mi Mundo Feliz” y relacionarlos con los logros de aprendizaje, durante el año 2013.  El estudio se realizó con una población total de 89 estudiantes en los salones de 3 y 4 años de edad, se realizó un muestro no probabilístico intencionado y se eligió 2 salones de cada grado, por lo que el estudio tuvo una muestra total de: 60 niños y niñas. El método utilizado en la investigación fue el no experimental, ya que no se manipuló ninguna variable, sino que fueron observadas en su normal desempeño, el tipo de investigación fue Descriptivo Correlacional, ya que se recogió datos para verificar si existe relación entre el Estado Nutricional y los logros de aprendizaje. Los resultados obtenidos se interpretaron por medio de cuadros y gráficos estadísticos, para la estadística descriptiva, se aplicó  la prueba de hipótesis de Chi2,  donde se obtuvo: X2c= 164,05 y X2t = 32,0, con una confiabilidad de 99%, por lo que al ser  X2c >X2t , se aceptó la hipótesis de investigación: El Estado Nutricional, tiene relación con los logros de aprendizaje en niños y niñas de la Institución Educativa Inicial Pública “Mi Mundo Feliz”, del distrito de Punchana, durante el año 2013

Impact of COVID-19 on the degree of compliance with hand hygiene: a repeated cross-sectional study

Hand hygiene (HH) is the paramount measure used to prevent healthcare associated infections. A repeated cross-sectional study was undertaken with direct observation of the degree of compliance on HH of healthcare personnel during the SARS-CoV-2 pandemic. Between, 2018-2019, 9,083 HH opportunities were considered, and 5,821 in 2020-2022. Chi squared tests were used to identify associations. The crude and adjusted odds ratios were used along with a logistic regression model for statistical analyses. Compliance on HH increased significantly (p < 0.001) from 54.5% (95% CI: 53.5, 55.5) to 70.1% (95% CI: 68.9, 71.2) during the COVID-19 pandemic. This increase was observed in four of the five key moments of HH established by the World Health Organization (WHO) (p<0.05), except at moment 4. The factors that were significantly and independently associated with compliance were the time period considered, type of healthcare-personnel, attendance at training sessions, knowledge of HH and WHO guidelines, and availability of hand disinfectant alcoholic solution in pocket format. Highest HH compliance occurred during the COVID-19 pandemic, reflecting a positive change in healthcare-personnel’s behavior regarding HH recommendations.We received funding through the Alicante Institute for Health and Biomedical Research (ISABIAL) plan for scientific and technical research and innovation project number 2021-0392

Global Entrepreneurship Monitor 2022/2023 Global Report : Adapting to a "New Normal"

Entrepreneurship, or the act of starting and running a new business, is a key catalyst of economic development. It is also an important driver of economic recovery: from the effects of the recent COVID-19 pandemic as well as more recent shocks, such as the war between Russia and Ukraine, with its related supply chain issues and rising energy costs. At any time, but especially during times of crisis, it is vital that the entrepreneurship dynamics and national frameworks to promote entrepreneurship are carefully defined and measured. Global Entrepreneurship Monitor (GEM) research can provide significant metrics to policymakers along the road to economic recovery, which enable informed and astute — but, most importantly, effective — decision-making. This 2022/2023 Global Report presents the results of GEM’s 24th research cycle. This adds another round of extensive national surveys to an already substantial GEM database of entrepreneurial results. In 2022, over 170,000 individuals were interviewed across 49 different economies, adding their views and experiences to over 3 million previously interviewed for the GEM Adult Population Survey (APS) over the previous two decades. These 49 economies represent about two-thirds of the global population in 2022. It includes China with a population of 1.3 billion, as well as India, which according to the United Nations is likely to be declared the world’s most populous country in 2023. Furthermore, GEM’s National Expert Survey (NES) features 51 economies (all of the 49 economies that participated in the GEM APS, plus Italy and Argentina). The NES is a survey of national experts in each economy charged with assessing the key components and characteristics of the entrepreneurial environment for that economy

Is Aboriginal Food Less Allergenic? Comparing IgE-Reactivity of Eggs from Modern and Ancient Chicken Breeds in a Cohort of Allergic Children

BACKGROUND: Hen's egg allergy ranks among the most frequent primary food allergies in children. We aimed to investigate sensitization profiles of egg allergic patients and compare in vitro IgE reactivities of eggs from ancient chicken breeds (Araucana and Maran) with those from conventional laying hen hybrids. METHODOLOGY: Egg allergic children (n = 25) were subjected to skin prick test, double blind placebo controlled food challenge, and sensitization profiles to Gal d 1-5 were determined by allergen microarray. IgE binding and biological activity of eggs from different chicken breeds were investigated by immunoblot, ELISA, and mediator release assays. PRINCIPAL FINDINGS: We found that Gal d 1 and Gal d 2 are generally major egg allergens, whereas Gal d 3-5 displayed high sensitization prevalence only in patients reacting to both, egg white and yolk. It seems that the onset of egg allergy is mediated by egg white allergens expanding to yolk sensitization in later stages of disease. Of note, egg white/yolk weight ratios were reduced in eggs from Auraucana and Maran chicken. As determined in IgE immunoblots and mass analysis, eggs from ancient chicken breeds did not differ in their protein composition. Similar IgE-binding was observed for all egg white preparations, while an elevated allergenicity was detected in egg yolk from Araucana chicken. CONCLUSION/SIGNIFICANCE: Our results on allergenicity and biological activity do not confirm the common assumption that aboriginal food might be less allergenic. Comprehensive diagnosis of egg allergy should distinguish between reactivity to hen's egg white and yolk fractions to avoid unnecessary dietary restrictions to improve life quality of the allergic child and its family

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P &lt; 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Burnout among surgeons before and during the SARS-CoV-2 pandemic: an international survey

Background: SARS-CoV-2 pandemic has had many significant impacts within the surgical realm, and surgeons have been obligated to reconsider almost every aspect of daily clinical practice. Methods: This is a cross-sectional study reported in compliance with the CHERRIES guidelines and conducted through an online platform from June 14th to July 15th, 2020. The primary outcome was the burden of burnout during the pandemic indicated by the validated Shirom-Melamed Burnout Measure. Results: Nine hundred fifty-four surgeons completed the survey. The median length of practice was 10&nbsp;years; 78.2% included were male with a median age of 37&nbsp;years old, 39.5% were consultants, 68.9% were general surgeons, and 55.7% were affiliated with an academic institution. Overall, there was a significant increase in the mean burnout score during the pandemic; longer years of practice and older age were significantly associated with less burnout. There were significant reductions in the median number of outpatient visits, operated cases, on-call hours, emergency visits, and research work, so, 48.2% of respondents felt that the training resources were insufficient. The majority (81.3%) of respondents reported that their hospitals were included in the management of COVID-19, 66.5% felt their roles had been minimized; 41% were asked to assist in non-surgical medical practices, and 37.6% of respondents were included in COVID-19 management. Conclusions: There was a significant burnout among trainees. Almost all aspects of clinical and research activities were affected with a significant reduction in the volume of research, outpatient clinic visits, surgical procedures, on-call hours, and emergency cases hindering the training. Trial registration: The study was registered on clicaltrials.gov "NCT04433286" on 16/06/2020