The effect of aerobic exercise after exposure to black carbon particulate matter 2.5 on expression of NF-κB and TNF-α genes in the lung tissue of male rats

Background: The aim of the present study was to investigate the effect of aerobic exercise after exposure to black carbon particulate matter 2.5 (PM 2.5) on expression of NF-κB, and TNF-α genes in the lung tissue of male rats. Materials and Methods: Twenty-four male Wistar rats (mean weight, 279.29±26.97 g) were randomly divided into four equal groups: 1) control (without any intervention), 2) without aerobic exercise and with exposure to PM2.5 black carbon, 3) aerobic exercise without exposure to black carbon, and 4) aerobic exercise after exposure to PM2.5 black carbon. The aerobic exercise protocol was performed at 50 the maximal speed of each group for 4 weeks 15 minutes after exposure to black carbon particles in the chamber. Animals were sacrificed 24 hours after the last training session and exposure to black carbon particles. The expression of NF-κB and TNF-α genes were analyzed in the lung tissue of rats by the real-time PCR. Results: A significant difference was found in the NF-κB expression between the group 1 and other groups and also there was a significant difference in the TNF-α expression between the group 2 and group 3 after exposure to black carbon in the lung tissue of male rats. Conclusion: The findings show that aerobic exercise after exposure to the black carbon particles (PM2.5) is associated with adjustment in lung inflammatory factors. It seems that these changes in part are associated with a lower risk of inflammatory pulmonary disease

Potential Improvement in Rehabilitation Quality of 2019 Novel Coronavirus by Isometric Training System; Is There “Muscle-Lung Cross-Talk”?

The novel Coronavirus Disease 2019 (COVID-19) crisis is now present in more than 200 countries. It started in December 2019 and has, so far, led to more than 149, 470,968 cases, 3,152,121 deaths, and 127,133,013 survivors recovered by 28 April 2021. COVID-19 has a high morbidity, and mortality of 2%, on average, whereas most people are treated after a period of time. Some people who recover from COVID-19 are left with 20 to 30% decreased lung function. In this context, exercise focused on skeletal muscle with minimal lung involvement could potentially play an important role. Regular exercise protects against diseases associated with chronic low-grade systemic inflammation. This long-term effect of exercise may be ascribed to the anti-inflammatory response elicited by an acute bout of exercise, which is partly mediated by muscle-derived myokines. The isometric training system seems to have this feature, because this system is involved with the skeletal muscle as the target tissue. However, no studies have examined the effect of exercise on the treatment and recovery of COVID-19, and, more importantly, "muscle-lung cross-talk" as a mechanism for COVID-19 treatment. It is suggested that this theoretical construct be examined by researchers

The Effects of Aerobic Exercise on NF-κB and TNF-α in Lung Tissue of Male Rat

Background:Regular aerobic exercise improves theBackground: Regular aerobic exercise improves the inflammatory status in different lung diseases. However, the effects of long-term aerobic exercise on the lung response have not been investigated. The present study evaluated the effect of aerobic exercise on the lung inflammatory.Materials and Methods: 12 adult male Wistar rats were divided to 2 groups: A: control (n=6), B: aerobic exercise (five times per week for 4 week; n=6). The gene expression of NF-κB and TNF-α were analyzed in lung tissue by Real time–PCR. In order to determine the significant differences between groups independent t-test were used.Results: Aerobic exercise inhibited the gene expression of NF-κB and TNF-α. But there was no significant difference between A and B groups for TNF-α and NF-κB.Conclusion: We conclude that four week aerobic exercise decrease inflammatory status in lung tissue. Our results indicate a need for human studies that evaluate the lung responses to aerobic exercise

Resistance exercise in a hot environment alters serum markers in untrained males

Purpose:We examined the effects of moderate resistance exercise (RE) on serum cortisol, testosterone, extracellular heat shock protein (HSP70), and interleukin (IL)-6 and IL-15 concentrations in untrained males in a hot environment. Methods:Ten untrained young males (26 +/- 3 years; 75.8 +/- 6 kg; 177.4 +/- 5.3 cm) performed two series of full body RE [3 sets of 8 to 10 repetitions, 30-60 s recovery between series with 70% of one maximal repetition (1-RM), with a rest period of 1 to 3 min between exercises] carried out in a random order in both heated (similar to 35 degrees C) and thermoneutral (22 degrees C) conditions. Serum concentrations of testosterone, cortisol, HSP70, and IL-6 and IL-15 were measured before, at the end, and 1 h after RE sessions. Participants in both groups consumed 4 ml of water/kg body mass every 15 min. Results:There were time-related changes in testosterone, HSP70, and IL-6 (P 0.05). Conclusion:RE in a heated environment may not be appropriate for achieving muscle adaptations due to acute changes of hormonal and inflammatory markers

Effect of acute Ramadan fasting on muscle function and buffering system of male athletes

The aim of this study was to investigate the effect of acute Ramadan fasting (RF) on the muscle function and buffering system. Twelve male athletes with 8 years of professional sports experience (age, 23.2 ± 1.3 years, body mass index: 24.2 ± 2.2 kg/m2) participated in this study. The subjects were tested twice, 3 weeks after the beginning of RF and 2 weeks after the end RF. Muscle function, buffering capacity, and rating of perceived exertion (RPE) were measured during and after RF by using the Biodex isokinetic machine, blood gas analyzer, and RPE 6-20 Borg scale, respectively. Venous blood samples for pH and bicarbonate (HCO3-) were measured during and after RF by using the Biodex isokinetic machine, blood gas analyzer, and RPE 6-20 Borg scale, respectively. Venous blood samples for pH and bicarbonate (HCO3-) were taken immediately after 25 repetitions of isokinetic knee flexion and extension. Measures taken during isokinetic knee extension during RF were significantly lower than those after RF in extension peak torque (t = -4.72, p = 0.002), flexion peak torque (t = -3.80, p = 0.007), extension total work (t = -3.05, p = 0.019), extension average power (t = -4.20, p = 0.004), flexion average power (t = -3.37, p = 0.012), blood HCO3- (t = -2.02, p = 0.041), and RPE (Z = -1.69, p = 0.048). No influence of RF was found on the blood pH (t = 0.752, p = 0.476). RF has adverse effects on muscle function and buffering capacity in athletes. It seems that a low-carbohydrate substrate during RF impairs muscle performance and reduces the buffering capacity of the blood, leading to fatigue in athletes

Resistance Exercise in a Hot Environment Alters Serum Markers in Untrained Males

[EN] Purpose: We examined the effects of moderate resistance exercise (RE) on serum cortisol, testosterone, extracellular heat shock protein (HSP70), and interleukin (IL)-6 and IL-15 concentrations in untrained males in a hot environment. Methods: Ten untrained young males (26 ± 3 years; 75.8 ± 6 kg; 177.4 ± 5.3 cm) performed two series of full body RE [3 sets of 8 to 10 repetitions, 30–60 s recovery between series with 70% of one maximal repetition (1-RM), with a rest period of 1 to 3 min between exercises] carried out in a random order in both heated (∼35◦C) and thermoneutral (22◦C) conditions. Serum concentrations of testosterone, cortisol, HSP70, and IL-6 and IL-15 were measured before, at the end, and 1 h after RE sessions. Participants in both groups consumed 4 ml of water/kg body mass every 15 min. Results: There were time-related changes in testosterone, HSP70, and IL-6 (P < 0.001), and cortisol and IL-15 (P < 0.05). Levels of cortisol, HSP70, and IL- 6 increased immediately for RE at 35◦C, and testosterone and IL-15 levels were decreased. Changes in serum testosterone, HSP70, cortisol, and IL-15 and IL-6 levels were reversed after 1 h. A significant time × condition interaction was observed for IL-15 and HSP70 (P 0.05). Conclusion: RE in a heated environment may not be appropriate for achieving muscle adaptations due to acute changes of hormonal and inflammatory marker

Air Pollution, Exercise, and Inflammation

Sports activities are hindered by the lack of clean air in large and industrialized cities, and the countless benefits of exercise are not considered. With the search of the literature, 89094 articles related to air pollution that have been published in SID, PubMed, and Web of Science until December 2021 were found. Ultimately, 80 articles were extracted after selecting the articles and narrowing the search to exercise inflammation and lung tissue. After removing out-of-date articles (not published between 2000 and 2021) and those without full text, 65 articles were included in the review. Based on the review, exposure to air pollution is associated with increased inflammation and decreased cellular immune function, which is characterized by the activation of toll-like receptors and downstream signaling pathways. Regular exercise improves the inflammatory status in different pulmonary diseases. People who exercise in polluted environments are at increased risk of respiratory and cardiovascular diseases due to the elevated amount of pollutants delivered to the lungs. However, studies did not take into account the potential anti-inflammatory effects of exercise training, which could inhibit the pro-inflammatory events induced by air pollution. It seems that regular exercise modifies pulmonary inflammatory responses and body mass and in part decreases the risk of pulmonary diseases

Effect of aerobic training after long-term inhalation of black carbon particles on IL-β gene expression in the lung tissue of male rats: brief report

Background: Air pollution PM exposure associated with increased inflammation and decreased cellular immune function. The innate immune system is one of the first lines of defense against inhaled air pollution and is characterized by activation of lung key signaling pathways. One pathway is initiated by the activation of toll-like receptors (TLRs) that associated with airway cells culminates in the translocation of nuclear factor-kappa B and other transcription factors to the nucleus, and therefore initiation of altered signaling of pro-inflammatory mediators such as IL-1&beta; and TNF-&alpha;. The purpose of this study was to investigate the effect of 4 weeks of aerobic training after long-term inhalation of black carbon black particulate matter on IL-&beta; lung tissue in male rats. Methods: The present study was conducted experimentally in October and November 2015 at the Sport Physiology Laboratory of Tarbiat Modares University of Tehran. 12 adult male Wistar rats (279.29&plusmn;26.97) were divided to two groups: A: Daily, two hours inhalation of carbon black PM10, n=6), B: 60 minutes aerobic exercise activity, 50 percent average peak velocity, 15 minutes after inhalation of carbon black PM10. The gene expression of IL-1&beta; was analyzed in lung tissue by real-time-PCR and Pfaffl formula. In order to determine the significant differences between groups independent t-test were used. Results: Although, there were no significant differences among the A and B groups, however, the mean of relative gene expression of IL-1&beta; in group B was slightly lower than group A. Conclusion: Four weeks regular aerobic exercise activity did not worsen lung tissue inflammation after long-term inhalation of carbon black PM10. It seems regular exercise training mitigate pulmonary inflammatory responses and in part, decrease the risk of pulmonary diseases

PENGARUH METODE PEMBELAJARAN TUTOR SEBAYA TERHADAP KETERAMPILAN DRIBBLING DALAM PERMAINAN SEPAK BOLA PADA SISWA EKSTRAKURIKULER

Dalam sebuah pembelajaran sangatlah penting untuk menggunakan metode pembelajaran untuk meningkatkan kualitas pembelajaran. sebuah metode pembelajaran diperlukan untuk mempermudah pembelajaran sehinggaga dapat membantu meningkatkan pembelajaran. Salah satunya seperti metode pembelajaran tutor sebaya yang merupakan sebuah metode pembelajaran yang menggunakan teman sebaya sebagai tutor atau pembimbing dalam sebuah proses pembelajaran. berdasarkan asumsi tersebut, penelitian ini memuat tentang bagaimana kontribusi dan pengaruh metode pembelajaran tutor sebaya. Adapun untuk lebih jelasnya penelitian ini berjudul “Pengaruh Metode Pembelajaran Tutor Sebaya Terhadap Keterampilan Dribbling Dalam Permainan Sepak Bola Pada Siswa Ekstrakurikuler” dari paparan tersebut penelitian ini bertujuan mendeskripsikan besar kontribusi dan pengaruh dari mpenggunaan metode pembelajaran tutor sebaya terhadap keterampilan dribbling dalam permainan sepak bola. Metode penelitian yang digunakan adalah metode penelitian kuantitatif eksperimen dengan desain satu kelompok yaitu Pre eksperimen desain one group pretest-postest design. Adapun sampel yang digunakan dalam penelitian ini adalah seluruh siswa yang mengikuti program ekstrakuriler di SDN 1 Bangodua Kecamatan Klangenan kabupaten Cirebon. Hasil dari penelitian ini menunjukkan jika terdapat adanya kontribusi dari penggunaan metode pembelajaran tutor sebaya terhadap keterampilan dribbling dalam permainan sepak bola pada siswa ekstrakurikuler. Dan juga penggunaan metode pembelajaran tutor sebaya memiliki pengaruh terhadap keterampilan dribbling dalam permainan sepak bola pada siswa ekstrakurikuler. Sehingga dapat disimpulkan bahwa penggunaan metode pembelajaran tutor sebaya memiliki kontribusi dan juga berpengaruh terhadap keterampilan dribbling dalam permainan sepak bola pada siswa ekstrakurikuler. Kata Kunci: Metode Pembelajaran, Tutor Sebaya, Kontribusi, Pengaruh

The effect of aerobic exercise after exposure to black carbon particulate matter 2.5 on expression of NF-κB and TNF-α genes in the lung tissue of male rats

Background: The aim of the present study was to investigate the effect of aerobic exercise after exposure to black carbon particulate matter 2.5 (PM 2.5) on expression of NF-κB, and TNF-α genes in the lung tissue of male rats. Materials and Methods: Twenty-four male Wistar rats (mean weight, 279.29±26.97 g) were randomly divided into four equal groups: 1) control (without any intervention), 2) without aerobic exercise and with exposure to PM2.5 black carbon, 3) aerobic exercise without exposure to black carbon, and 4) aerobic exercise after exposure to PM2.5 black carbon. The aerobic exercise protocol was performed at 50% the maximal speed of each group for 4 weeks 15 minutes after exposure to black carbon particles in the chamber. Animals were sacrificed 24 hours after the last training session and exposure to black carbon particles. The expression of NF-κB and TNF-α genes were analyzed in the lung tissue of rats by the real-time PCR. Results: A significant difference was found in the NF-κB expression between the group 1 and other groups and also there was a significant difference in the TNF-α expression between the group 2 and group 3 after exposure to black carbon in the lung tissue of male rats. Conclusion: The findings show that aerobic exercise after exposure to the black carbon particles (PM2.5) is associated with adjustment in lung inflammatory factors. It seems that these changes in part are associated with a lower risk of inflammatory pulmonary disease