Proposal for the application of ICE and BIM sessions to increase productivity in construction

Several studies have shown that the main problem in the construction industry is low productivity. Therefore, this study focuses on developing a proposal through a methodology that can increase productivity in the construction of buildings. The proposed methodology is Virtual Design and Construction (VDC), which has 4 pillars: Building Information Modeling (BIM), Integrated Concurrent Engineering (ICE), metrics and Project Production Management (PPM). However, the article mainly develops BIM and ICE sessions. In addition, in the ICE sessions, "work executors"will be added, so that the information is fed back by both parties, specialist engineers and work executors. Finally, the proposal will be applied in a multi-family building project in the city of Lima - Peru, in order to obtain improvement results

Structural Characteristics Of The Tendinous Cord-papillary Muscle Junction In Healthy And Hypertensive Rats

Although the myotendinous junction (MTJ) of skeletal striated muscle is well known, more detailed studies regarding the structure of the cardiac MTJ are scarce. The objective of the present study was to investigate the morphological characteristics of the MTJ in hearts of healthy and hypertensive (SHR) female rats using histological, ultrastructural and three-dimensional (SEM) methods, as well as to evaluate the expression of vinculin by immunofluorescence. In the two groups, light microscopy showed branching tendinous cords and collagen bundles penetrating the apex of the finger-like projections of the papillary muscle. SEM analysis revealed an enlarged apex of the papillary muscle in SHR which was not observed in healthy animals. The loss of force transmission appears to be compensated by the amplified connection between the papillary muscle and valvular collagen. A large number of intercalated disks close to the fiber apex, small amounts of an amorphous intercellular substance and numerous vesicles were observed in SHR. In these animals, the expression of vinculin was more marked showing a regular distribution and a pattern of transverse striations along the sarcolemma. The presence of this protein in transverse bands suggests that vinculin surrounds myofibrils in the region of the Z band. Vinculin staining was also more marked in the region of the tendinous cord-papillary muscle junctions of SHR compared to control animals. Vinculin was quantified by electrophoresis and higher amounts of this protein were observed in SHR compared to control animals. © 2009 Elsevier Ltd. All rights reserved.407691701Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K., (1999) Fundamentos da Biologia Celular, , Artes Médicas, Porto AlegreAnastasi, G., Amato, A., Tarone, G., Vita, G., Monici, M.C., Magaudda, L., Distribution and localization of vinculin-talin-integrin system dystrophin-glycoprotein complex in human skeletal muscle. Immunohistochemical study using confocal laser scanning microscopy (2003) Cells Tissues Organs, 175 (3), pp. 151-164Bailly, M., Connecting cell adhesion to the actin polymerization machinery: vinculin as the missing link? (2003) Trends Cell Biol., 13 (4), pp. 163-165Barstead, R.J., Waterston, R.H., Vinculin is essential for muscle function in the nematode (1991) J. Cell Biol., 114 (4), pp. 715-724Cotran, R.S., Kumar, V., Collins, T., (2000) Robbins Pathologic Basis of Disease, , Saunders, PhiladelphiaDulhunty, A.F., Franzini-Armstrong, C., The relative contributions of the folds and caveolae to the surface membrane of frog skeletal muscle fibers at different sarcomere lengths (1975) J. Physiol., 250 (3), pp. 513-539Fawcett, D.W., (1969) An Atlas of Fine Structure, , W.B. Saunders Company, Philadelphia and LondonFranzini-Armstrong, C., Landmesser, L., Pilar, G., Size and shape of transverse tubule openings in frog twitch muscle fibers (1975) J. Cell Biol., 64 (2), pp. 493-497Icardo, J.M., Colvee, E., Atrioventricular valves of the mouse: III. Collagenous skeleton and myotendinous junction (1995) Anat. Rec., 243 (3), pp. 367-375Law, D.J., Ultrastructural comparison of slack of stretched myotendinous junctions, based on a three-dimensional model of the connecting domain (1993) J. Muscle Res. Cell Motil., 14 (4), pp. 401-411Mackay, B., Harrop, T.J., Muir, A.R., The fine structure of the muscle tendon junction in the rat (1969) Acta Anat., 73 (4), pp. 588-602Mair, W.G., Tome, F.M., The ultrastructure of the adult and developing human myotendinous junction (1972) Acta Neuropathol., 21 (3), pp. 239-252Massa, R., Silvestri, G., Sancesario, G., Bernardi, G., Immunocytochemical localization of vinculin in muscle and nerve (1995) Muscle Nerve, 18 (11), pp. 1277-1284Nakao, T., Fine structure of the myotendinous junction and "terminal coupling" in the skeletal muscle of the lamprey, Lampetra japonica (1975) Anat Rec., 182 (3), pp. 321-337Nakao, T., Some observations on the fine structure of the myotendinous junction in myotomal muscle of the tadpole tail (1976) Cell Tissue Res., 166 (2), pp. 241-254Porter, G.A., Dmytrenko, G.M., Winkelmann, J.C., Bloch, R.J., Distrophin colocalizes with β-spectrin in distinct subsarcolemmal domains in mammalian skeletal muscle (1992) J. Cell Biol., 117 (5), pp. 997-1005Ross, M.H., Romrell, L.J., Kaye, G.I., (1995) Histology: A Text and Atlas, , Williams and Wilkins, BaltimoreShear, C.R., Bloch, R.J., Vinculin in subsarcolemal densities in chicken muscle: localization and relationship to intracellular and extracellular structures (1985) J. Cell Biol., 101 (1), pp. 240-256Terracio, L., Simpson, D.G., Hilenski, L., Carver, W., Decker, R.S., Vinson, N., Distribution of vinculin in the Z-disk of striated muscle: analysis by laser scanning confocal microscopy (1990) J. Cell Physiol., 145 (1), pp. 78-87Tidball, J.G., Myotendinous junction: morphological changes and mechanical failure associated with muscle cell atrophy (1984) Exp. Mol. Pathol., 40 (1), pp. 1-12Tidball, J.G., Law, D.J., Dystrophin is required for normal thin filament-membrane associations at myotendinous junctions (1991) Am. J. Pathol., 138 (1), pp. 17-21Tidball, J.G., Chan, M., Adhesive strength of single muscle cells to basement membrane at myotendinuos junctions (1989) J. Appl. Physiol., 67 (3), pp. 1063-1069Tidball, J.G., Daniel, T.L., Myotendinous junctions of tonic muscle cells: structure and loading (1986) Cell Tissue Res., 245 (2), pp. 315-322Trotter, J.A., Eberhard, S., Samora, A., Structural domains of the muscle-tendon junction. 1. The internal lamina and the connecting domain (1983) Anat. Rec., 207 (4), pp. 573-591Trotter, J.A., Corbett, K., Avner, B.P., Structure and function of the murine muscle-tendon junction (1981) Anat. Rec., 201 (2), pp. 293-302Williams, P., Warwick, R., (1995) Gray's Anatomy, , Guanabara Koogan, Rio de JaneiroZar, J.H., (1999) Biostatistical Analysis, , Prentice-Hall, New Jerse

Morphological and morphometric study of the opossum's dorsal root ganglia neurons

The ultrastructural characteristics and the morphometric evaluation of the different types of neurons present in the dorsal root ganglia (DRG) of the South American opossum (Didelphis albiventris) were studied. Four adult male animals were used and the neurons from cervical and lumbar DRG were removed and processed for histological and transmission electron microscopy observations. The morphometric data were obtained from serial sections stained by H/E and Masson's trichrome. The number of neurons in cervical and lumbar DRG was 22 300 and 31 000, respectively. About 68% of the cervical neurons and 62.5% of the lumbar neurons presented areas up to 1300 mu m(2) and were considered as the small neurons of the DRG. The ultrastructural observations revealed two morphological types of neurons: clear large neurons and dark small neurons. The nuclei of both cell types are spherical and the chromatin is disperse and rarefected. The cytoplasm of the dark small neuron is more electron dense and shows a regular distribution of small mitochondria and many rough reticulum cisterns in the periphery. A small Golgi apparatus was close to the nucleus and many disperse neurofilaments occupy most parts of the cytoplasm. Smooth reticulum cisterns are rare and lipofucsin-like inclusions are present at some points. In the clear large neurons, the organelles are homogenously scattered through the cytoplasm. The neurofilaments are close packed forming bundles and small mitochondria and rough reticulum cisterns are disperse. Lipofucsin-like inclusions are more frequent in these cells

Morphologic alterations resulting from denervation of the diaphragm in rats

The morphological alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into two fragments, which were used for histological (RE.) and histoenzymological (NADH-TR and myofibrillar ATPase). After 4 weeks, denervated muscle fibers showed important light microscopic alterations: atrophy with angular profiles in cross-sections, cytoplasm containing vacuoles, enlarged interstitial space with increased connective tissue, cellular infiltration, and muscle fibers without defined contours. The most marked alterations being observed for type IIb and ha fibers. Eight and 12 weeks after denervation, the NADH-TR reaction showed that it was impossible to characterize the muscle fibers based on their metabolic profile.Fueron estudiadas las alteraciones morfológicas de las fibras musculares del diafragma denervado de ratas. Se utilizaron 15 ratas albinas (Rattus norvegicus) machos, adultos, con peso promedio de 200g y cerca de 60 días de edad. Se denervó el diafragma y después de 4, 8 y 12 semanas los animales fueron sacrificados. El antímero izquierdo del diafragma fue denervado por sección del nervio frénico y el antímero derecho fue utilizado como control. Cada antímero fue dividido en fragmentos, utilizados para el estudio histológico (H-E), histoenzimológico (NADH-TR y ATPasa miofibrilar). Después de 4 semanas las fibras musculares denervadas presentaron alteraciones importantes en lamicroscopía de luz: atrofia con perfiles angulados en secciones transversales; citoplasma con vacuolas; aumento del espacio intersticial con aumento de tejido conjuntivo; infiltración celular y fibras musculares sin contornos definidos, siendo las alteraciones más marcadas en las fibras tipo IIb e IIa. Después de 8 y 12 semanas de denervación la reacción para NADH-TR demuestra que es imposible caracterizar a las fibras musculares a través de su perfil metabólico.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Ultrastructural Study of the Denervated Diaphragm in Rats

The structural alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into fragments, which were used for analysis transmission electron microscopy. During the initial phase of denervation (4 weeks), ultrastructural muscle modifications appeared in scattered fibers and in foci along these fibers. Muscle fibers with foci of less dense and loosely arranged myofibrils, disorganized Z line, displaced T tubules, and central nucleus exhibiting reentrances and fragmented aspect were observed. After 8 weeks, formation of large aggregates of small elongated mitochondria showing altered cristae, matrix inclusions and increased electron density was noted. At 12 weeks of denervation the alterations were found to be more drastic. Nuclei with internal deposits of myofibrillar or amorphous material were observed. In these fibers, vacuoles harbored enormous myeloid structures in the subsarcolemmal or intermyofibrillar region.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Common values in assessing health outcomes from disease and injury: disability weights measurement study for the Global Burden of Disease Study 2010.

BACKGROUND: Measurement of the global burden of disease with disability-adjusted life-years (DALYs) requires disability weights that quantify health losses for all non-fatal consequences of disease and injury. There has been extensive debate about a range of conceptual and methodological issues concerning the definition and measurement of these weights. Our primary objective was a comprehensive re-estimation of disability weights for the Global Burden of Disease Study 2010 through a large-scale empirical investigation in which judgments about health losses associated with many causes of disease and injury were elicited from the general public in diverse communities through a new, standardised approach. METHODS: We surveyed respondents in two ways: household surveys of adults aged 18 years or older (face-to-face interviews in Bangladesh, Indonesia, Peru, and Tanzania; telephone interviews in the USA) between Oct 28, 2009, and June 23, 2010; and an open-access web-based survey between July 26, 2010, and May 16, 2011. The surveys used paired comparison questions, in which respondents considered two hypothetical individuals with different, randomly selected health states and indicated which person they regarded as healthier. The web survey added questions about population health equivalence, which compared the overall health benefits of different life-saving or disease-prevention programmes. We analysed paired comparison responses with probit regression analysis on all 220 unique states in the study. We used results from the population health equivalence responses to anchor the results from the paired comparisons on the disability weight scale from 0 (implying no loss of health) to 1 (implying a health loss equivalent to death). Additionally, we compared new disability weights with those used in WHO's most recent update of the Global Burden of Disease Study for 2004. FINDINGS: 13,902 individuals participated in household surveys and 16,328 in the web survey. Analysis of paired comparison responses indicated a high degree of consistency across surveys: correlations between individual survey results and results from analysis of the pooled dataset were 0·9 or higher in all surveys except in Bangladesh (r=0·75). Most of the 220 disability weights were located on the mild end of the severity scale, with 58 (26%) having weights below 0·05. Five (11%) states had weights below 0·01, such as mild anaemia, mild hearing or vision loss, and secondary infertility. The health states with the highest disability weights were acute schizophrenia (0·76) and severe multiple sclerosis (0·71). We identified a broad pattern of agreement between the old and new weights (r=0·70), particularly in the moderate-to-severe range. However, in the mild range below 0·2, many states had significantly lower weights in our study than previously. INTERPRETATION: This study represents the most extensive empirical effort as yet to measure disability weights. By contrast with the popular hypothesis that disability assessments vary widely across samples with different cultural environments, we have reported strong evidence of highly consistent results. FUNDING: Bill & Melinda Gates Foundation