Evaluation of diagnostic value of soluble urokinase-type plasminogen activator receptor in sepsis

Background: Sepsis is one of the most important causes of morbidity and mortality in the intensive care units (ICUs). It is difficult to accurately differentiate sepsis from similar diseases rapidly. Therefore, it becomes critical to identify any biomarker with the ability of differentiation between sepsis and nonsepsis conditions. The urokinase plasminogen activator receptor has been implicated as an important factor in regulation of leukocyte adhesion and migration. Objectives: In this study, we evaluated the value of soluble urokinase plasminogen activator receptor (suPAR), erythrocyte sedimentation (ESR), and C-reactive protein (CRP) serum levels in terms of their value for sepsis diagnosis in ICU patients. Patients and Methods: We enrolled 107 ICU patients; 40 with sepsis, 43 with systemic inflammatory response syndrome, and 24 as control group. Serum soluble urokinase plasminogen activator receptor, ESR, white blood cell (WBC), and CRP levels were measured on the day of admission. Results: The group with sepsis had higher suPAR, ESR, and CRP levels compared with the group with noninfectious systemic inflammatory response syndrome (SIRS) (P = 0.01, 0.00 and 0.00, respectively). CRP concentrations and ESR were higher in the sepsis group than in the non-SIRS group (P = 0.00 and 0.00, respectively). In a receiver-operating characteristic curve analysis, ESR, CRP and suPAR had an area under the curve larger than 0.65 (P = 0.00) in distinguishing between septic and noninfectious SIRS patients. CRP, ESR and suPAR had a sensitivity of 87, 71 and 66 and a specificity of 59, 76 and 74 respectively in diagnosing infection in SIRS. Conclusions: The diagnostic values of CRP and ESR were better than suPAR and WBC count in patients with sepsis. © 2015, Infectious Diseases and Tropical Medicine Research Center

Interference Alignment — Practical Challenges and Test-bed Implementation

Data traffic over wireless communication networks has experienced a tremendous growth in the last decade, and it is predicted to exponentially increase in the next decades. Enabling future wireless networks to fulfill this expectation is a challenging task both due to the scarcity of radio resources (e.g. spectrum and energy), and also the inherent characteristics of the wireless transmission medium. Wireless transmission is in general subject to two phenomena: fading and interference. The elegant interference alignment concept reveals that with proper transmission signalling design, different interference signals can in fact be aligned together, such that more radio resources can be assigned to the desired transmission. Although interference alignment can achieve a larger data rate compared to orthogonal transmission strategies, several challenges should be addressed to enable the deployment of this technique in future wireless networks For instance, to perform interference alignment, normally, global channel state information (CSI) is required to be perfectly known at all terminals. Clearly, acquiring such channel knowledge is a challenging problem in practice and proper channel training and channel state feedback techniques need to be deployed. In addition, since the channels are time-varying proper adaptive transmission is needed. This chapter review recent advances in practical aspects of interference alignment. It also presents recent test-bed implementations of signal processing algorithms for the realization of interference alignment.Comment: Book Chapter accepted for publication in the book entitled: Contemporary Issues in Wireless Communications, ISBN: 978-953-51-4101-3, Khatib, M. (Ed.), to be published by INTECH Publishers. Expected month of publication: November 201

Interference alignment testbeds

Interference alignment has triggered high impact research in wireless communications since it was proposed nearly 10 years ago. However, the vast majority of research is centered on the theory of interference alignment and is hardly feasible in view of the existing state-of-the-art wireless technologies. Although several research groups have assessed the feasibility of interference alignment via testbed measurements in realistic environments, the experimental evaluation of interference alignment is still in its infancy since most of the experiments were limited to simpler scenarios and configurations. This article summarizes the practical limitations of experimentally evaluating interference alignment, provides an overview of the available interference alignment testbed implementations, including the costs, and highlights the imperatives for succeeding interference alignment testbed implementations. Finally, the article explores future research directions on the applications of interference alignment in the next generation wireless systems.Jacobo Fanjul's research has been supported by the Ministerio de Economía y Competitividad (MINECO) of Spain, under grants TEC2013-47141-C4-R (RACHEL project) and FPI grant BES-2014-069786. José A. García-Naya's research has been funded by the Xunta de Galicia (ED431C 2016–045, ED341D R2016/012, E0431 G/01), the Agencia Estatal de Investigación of Spain (TEC2013-47141-C4-1-R, TEC2015-69648-REOC, TEC2016-75067-C4-1-R), and ERDF funds of the EU (AEI/FEDER, UE). Hamed Farhadi's research has been funded by the Swedish Research Council (VR) under grant 2015–00500

Design, construction and evaluation of potato digger with rotary blade

Potato has an important role in human food. It was the sixth alimentary product in the world after sugar cane, maze, rice, wheat and milk in 2011 year. In addition, potato is the third product in Iran after wheat and sugar cane in 2011 year. Therefore, any attempt in the improvement of potato harvester will be valuable. In this study, a new semi-mounted one-row potato digger with rotary blade was designed and made in the workshop of Shahrekord University. It can be connected to rotary potato graders. Transmission system was mechanical from tractor (PTO) to blade by belt, pulley, gearbox, chain and sprocket. Blade diameter was 76 cm and the length was 10 cm which was assigned by the researchers. For separating of soil from potato, a helix containing bars with 2.6 cm distance and diameter of 9 mm was applied. Entered soil into set was calculated as 227 ton/h. Required power was got 5.5 horsepower. Computerized model of set was prepared in Mechanical Desktop Software and potato motion was studied in Visual Nastran Software. The device was tested at field with various advance speed, blade angle and rotational speed. Results showed that advance speed of 1.5-3 km/h, rotational speed of 20-25 RPM and blade angle of 10- 15○ were proper for system. The average of damaged potatoes was 4%

Optimization and characterization of zeolite-titanate for ibuprofen elimination by sonication/hydrogen peroxide/ultraviolet activity

In this study, a photo-catalyst of titanium oxide was coated on zeolite by the sol–gel method. The generation of the zeolite-titanate photo-catalyst was optimized at conditions of calcination temperature (300, 350, 400 and 500 °C), calcination time (1, 2, 3, and 4 h), and titanate content (0, 2, 4, 6, and 8 mL). The catalyst was used for ‘Sonication/UV/H2O2″ activity and finally, eliminating ibuprofen. Physicochemical properties of the as-built photo-catalysts for all optimized conditions were determined using FESEM-EDX-mapping, BET, FTIR, and XRD. The highest percentage of ibuprofen removal (98.9%) was obtained at conditions of zeolite to titanium ratio of 1 g: 2 mL, time in the furnace of 1 h, and temperature of the furnace of 350 °C. The optimum photo-catalytic (namely, Cat-350-1-2) had a surface area value of 39 m2/g and a crystalline size of 4.9 nm. The surface area for all photo-catalysts increased after being used for ibuprofen removal, possibly due to ultrasonic waves. The presence of Ti-O, benzene ring, O-Al-O, O-Si-O, C–H, and O–H in the photo-catalysts structure were confirmed. Growing the calcination time resulted in an increase in the crystallinity of titanium dioxide in the photo-catalysts and, ultimately a reduction in the ibuprofen removal. The consumed energy by the developed system was calculated for the presence (0.094 kJ/g) and absence (17.5 kJ/g) of the ultrasonic wave. The degradation pathway and reaction kinetic are also explored and proposed. The results showed that the ultrasonic-UV-activated H2O2-based technique can be applied as an alternative method for ibuprofen removal from aqueous medi

Cloning of Vibrio cholerae outer membrane protein W in Pichia pastoris

BACKGROUND AND OBJECTIVE: The outer membrane protein W (ompW) of Vibrio cholerae is involved in stimulating the immune response via induction of protective immunity. It also plays an important role in bacterial pathogenesis by increasing the adaptability of pathogenic strains. In this study we aimed to clone V. cholerae ompW gene in the strain X-33 of Pichia pastoris. MATERIALS AND METHODS: A gene encoding ompW was cloned into the Ppicza vector downstream of alcohol oxidase promoter. Then recombinant vector was transformed into the genome of the strain X-33 of P. pastoris. After growth of zeocin-resistant transformants, clones were selected and subsequently confirmed for cloning by PCR enzymatic digestion and sequencing. RESULTS: PCR, enzymatic digestion and sequencing showed that the ompW gene was correctly cloned into P. pastoris genome. CONCLUSION: Results of our study showed that the methylotrophic yeast P. pastoris can be considered as an appropriate host instead of mammalian and prokaryotic systems for cloning of ompW. As far as data show, this is the first time that ompW of V. cholera is cloned into the methylotrophic P. pastoris

Association of interleukin 1 gene cluster and interleukin 1 receptor gene polymorphisms with ischemic heart failure

BACKGROUND: Proinfl ammatory cytokines have been known to play a considerable part in the pathomechanisms of chronic heart failure (CHF). Given the importance of proinfl ammatory cytokines in the context of the failing heart, we assessed whether the polymorphisms of interleukin (IL)-1 gene cluster, including IL-1a, IL-1β, and IL-1 receptor antagonist (IL-1RA) and IL-1R gene are predictors of CHF due to ischemic heart disease. METHODS: Forty- three patients with ischemic heart failure were recruited in this study as patients group and compared with 140 healthy unrelated control subjects. Using polymerase chain reaction with sequence-specifi c primers method, the allele and genotype frequency of 5 single nucleotide polymorphisms (SNPs) within the IL- 1a (-889), IL-1β (-511, +3962), IL-1R (psti 1970), and IL-1RA (mspa1 11100) genes were determined.RESULTS: The frequency of the IL-1β -511/C allele was signifi cantly higher in the patient group compared to that in the control group (p = 0.031). The IL-1β (-511) C/C genotype was signifi cantly overrepresented in patients compared to controls (p = 0.022). CONCLUSIONS: Particular allele and genotype in IL-1β gene were overrepresented in patients with ischemic heart failure, possibly affecting the individual susceptibility to this disease (Tab. 1, Ref. 27). Text in PDF www.elis.sk

Acoustic Analysis of Crying Signal in Infants with Disabling Hearing Impairment

Objective: Crying is a multimodal, dynamic behavior and the first way to communicate. Early identification of hearing impairment is critical for prevention of speech and language disorders. The present study aimed to assess the acoustic features of infant's cry signals to find possible differences between two groups including hearing-impaired (HI) infants and normal hearing (NH) control. Methods: The data were collected from 34 (17 HI, 17 NH) infants under 2 months of age. Recording of the infant cry signals was collected during the examination of the Babinski reflex and was subsequently submitted for acoustic analysis. The total duration of the recording for each infant was approximately 30 seconds. The acoustical features included fundamental frequency (F0), formants (F1, F2, and F3), intensity, jitter, shimmer, ratios of F2/F1 and F3/F1, ratio of harmonic to noise, and voice break. The recording device was an Olympus ws-321M voice recorder with 44,100 Hz sampling frequency in the stereo form. Praat analysis software (version 27, 3, 5) was used to analyze the crying signals. The data were then statistically analyzed using SPSS version 21. Results: Acoustic analysis of the crying signals showed that HI infants have lower intensity and higher F0 and voice break than NH infants. However, the other differences were not statistically significant. Conclusion: The results of the present study demonstrated that the acoustic components including F0, intensity, and voice break may be used as indices to discriminate HI infants from NH infants under 2 months of age. These findings can be increased our knowledge concerning the functional mechanisms of the vocal organ in HI and NH infants. © 201

Diagnostic performance of brain natriuretic peptide in patients suspected to Sepsis

Background: One of the main components involving in the pathogenesis and prognosis of sepsis and septic shock is natriuretic peptide. Hemodynamic changes in sepsis or septic shock might explain increased plasma levels of these peptides circulations. Objectives: The present study aimed to assess the value of brain natriuretic peptide (BNP) in prediction of sepsis state and its related morbidity. We also attempted to determine the best cutoff point of BNP for diagnosis of sepsis state in those with critical illnesses, admitted to the hospital. Patients and Methods: In a cross-sectional study on critically ill patients referred to infections unit of Hazrat Rasoul Akram Hospital, the enrolled patients were assigned to one of the sepsis (n = 30) and nonsepsis (n = 30) groups, based on their results of blood culture and clinical manifestations. Plasma level of BNP was measured by immunoassay. Results: In-hospital mortality was only observed in one patient suffering from sepsis. Hospital length of stay (LOS) was significantly longer in the sepsis group compared with the nonsepsis one (17.47 ± 10.10 days versus 7.93 ± 2.92 days). The plasma BNP level, as a marker, was significantly higher in the sepsis group than the nonsepsis one (786.87 ± 164.11 ng/mL versus 154.57 ± 44.67 ng/mL, P = 0.039). The BNP level was significantly correlated with some baseline variables including respiratory rate (beta = 0.295, P = 0.022) and ESR measurement (beta = 0.296, P = 0.022), but not with other characteristics. Through multivariable linear regression analysis, having other patients' variables information including demographics and hemodynamic parameters, the BNP level was significantly higher in the sepsis group compared with the nonsepsis one (odds ratio = 1.008, P = 0.046). According to the receiver operating characteristic (ROC) curve analysis, BNP measurement had an acceptable value for discriminating sepsis and nonsepsis states (c = 0.734, 95 CI: 0.605-0.864, P = 0.002). The optimal cutoff point of BNP for discriminating sepsis and nonsepsis states was 170 ng/mL, yielding a sensitivity of 66.7 and a specificity of 60.0. Conclusions: Elevated BNP level was associated with a significantly increased risk of sepsis state in critically ill patients. © 2013, Infectious Diseases and Tropical Medicine Research Center