Linkages between the southern Patagonia Pre-Permian basements : new insights from detrital zircons U-Pb SHRIMP ages from the Cerro Negro District

The Patagonian basement rocks are dominated by Precambrian to Early Paleozoic metamorphic rocks intruded by Paleozoic granitoids. Recently discovered basement rocks in the Cerro Negro District are characterized mainly by quartz-muscovite-chlorite schists; the metamorphic grade reaches greenschist facies (biotite-garnet grade) with a regional S1 schistosity subparallel to the original sedimentary structure S0 and a secondary non-penetrative S2 foliation. New detrital zircon U-Pb geochronology shows that maximum depositional ages for detrital zircons are Devonian, ages of 379 ± 4Ma. These results suggest that the Cerro Negro basement rocks are the youngest basement in the Deseado Massif, overlapping some detrital zircon ages in the eastern Andean Metamorphic Complex in the Andean region. Most of detrital zircons are igneous in origin with a major peak around ~396Ma, provably sourced from the Devonian granitoids of the Río Deseado Complex (El Laurel and Bahía Laura granites) and equivalent northern Patagonia granitoids (e.g. Colan Conhué and Lago Lolog granites). Secondary peaks correspond to Ordovician to Silurian ages, being the Río Deseado Complex and La Modesta Formation (and their igneous contributors) the possible sources of the zircons. The minor oldest peaks yield Cambrian-Neoproterozoic; Mesoproterozoic and Paleoproterozoic-Archean ages, evidencing a common source from the interior of Gondwana. The results provide new insights about the relationships between the pre-Permian metasedimentary rocks of the extra-Andean and Andean region during Mid-Paleozoic ages

Development and application of a novel Peptide Nucleic Acid probe for the specific detection of Cronobacter (Enterobacter sakazakii) in powdered infant formula

Cronobacter spp. are causative agents of meningitis, septicemia and necrotizing enterocolitis in neonates and immunocompromised infants. Recently, contaminated powdered infant formula (PIF) has been reported as a source of these infections. In order to minimize the risk of infection, the development of a rapid, sensitive and specific method for the early detection of this bacterium in infant formula is of the utmost importance. Fluorescence in situ hybridization (FISH), a technique that allows direct visualization of whole cells, has been combined with specific peptide nucleic acid (PNA) probes, a new synthetic molecule with a better hybridization performance than DNA probes. In this work, a new FISH method for the detection of Cronobacter spp. using a novel PNA probe is reported. This PNA-FISH method was then adapted for the detection of this bacterium in PIF. The PNA-FISH procedure using the Cronobacter probe proved to be a reliable method for the detection of this pathogen in PIF samples and an alternative to existing molecular methods. It presented high specificity and sensitivity, detected less than 1 CFU per 10g of Cronobacter in infant formula and provided detection in less than 12 hours. Direct visualization of bacterial cells was possible and the method was simple and easy to use, without any special equipment apart from an epifluorescence microscope. The samples can be also analysed by flow cytometry

Proterozoic crustal evolution of central East Antarctica: Age and isotopic evidence from glacial igneous clasts, and links with Australia and Laurentia

Rock clasts entrained in glacial deposits sourced from the continental interior of Antarctica provide an innovative means to determine the age and composition of ice-covered crust. Zircon U-Pb ages from a suite of granitoid clasts collected in glacial catchments draining central East Antarctica through the Transantarctic Mountains show that crust in this region was formed by a series of magmatic events at ∼2.01, 1.88–1.85, ∼1.79, ∼1.57, 1.50–1.41, and 1.20–1.06 Ga. The dominant granitoid populations are ca. 1.85, 1.45 and 1.20–1.06 Ga. None of these igneous ages are known from limited outcrop in the region. In addition to defining a previously unrecognized geologic history, zircon O and Hf isotopic compositions from this suite have: (1) mantle-like δ18O signatures (4.0–4.5‰) and near-chondritic Hf-isotope compositions (εHf ∼ +1.5) for granitoids of ∼2.0 Ga age; (2) mostly crustal δ18O (6.0–8.5‰) and variable Hf-isotope compositions (εHf = −6 to +5) in rocks with ages of ∼1.88–1.85, ∼1.79 and ∼1.57 Ga, in which the ∼1.88–1.79 Ga granitoids require involvement of older crust; (3) mostly juvenile isotopic signatures with low, mantle-like δ18O (∼4–5‰) and radiogenic Hf-isotope signatures (εHf = +6 to +10) in rocks of 1.50–1.41 Ga age, with some showing crustal sources or evidence of alteration; and (4) mixed crustal and mantle δ18O signatures (6.0–7.5‰) and radiogenic Hf isotopes (εHf = +3 to +4) in rocks of ∼1.2 Ga age. Together, these age and isotopic data indicate the presence in cratonic East Antarctica of a large, composite igneous province that formed through a punctuated sequence of relatively juvenile Proterozoic magmatic events. Further, they provide direct support for geological correlation of crust in East Antarctica with both the Gawler Craton of present-day Australia and Proterozoic provinces in western Laurentia. Prominent clast ages of ∼2.0, 1.85, 1.57 and 1.45 Ga, together with sediment source linkages, provide evidence for the temporal and spatial association of these cratonic elements in the Columbia supercontinent. Abundant ∼1.2–1.1 Ga igneous and metamorphic clasts may sample crust underlying the Gamburtsev Subglacial Mountains, indicating the presence of a Mesoproterozoic orogenic belt in the interior of East Antarctica that formed during final assembly of Rodinia.Field and analytical portions of this project were supported by the National Science Foundation (award 0944645)

Burdigalian deposits of the Santa Cruz Formation in the Sierra Baguales, Austral (Magallanes) Basin: Age, depositional environment and vertebrate fossils

Indexación: Web of Science; Scielo.ABSTRACT. A succession of marine and continental strata on the southern flank of Cerro Cono in the Sierra Baguales, northeast of Torres del Paine, can be correlated with stratigraphic units exposed along the southern border of the Lago Argentino region in Santa Cruz Province, Argentina. These include the Estancia 25 de Mayo Formation and the basal part of the Santa Cruz Formation. The lithological correlation is also confirmed by detrital zircon ages (maximum age of 18.23±0.26 Ma) and a rich assemblage of terrestrial vertebrate fossils, biostratigraphically equivalent to a postColhuehuapian, pre-Santacrucian South American Land Mammal Age (SALMA) fauna, suggesting a range of 19 to 17.8 Ma. Similar ages have been obtained from the basal part of the Santa Cruz Formation at Estancia Quién Sabe in southwestern Argentina, supporting the assumption of a regional continuity between these deposits. A measured lithostratigraphic column is presented and the depositional environment is interpreted as a coastal plain with small, meandering rivers and ephemeral floodplain lakes. The sedimentation coincides with intensified uplift of the Patagonian Andes during the ‘Quechua Phase’ of Andean tectonism, which is reflected by a change in paleocurrent directions from northwest to east-northeast. Keywords: Burdigalian, Santa Cruz Formation, Santacrucian SALMA, ‘Notohippidian’ fauna, Meandering rivers.RESUMEN. Una sucesión de estratos marinos y continentales en el flanco meridional del cerro Cono, en la sierra Baguales, al noreste de Torres del Paine, se correlaciona con estratos al sur de la región de lago Argentino en la Provincia de Santa Cruz, República Argentina. Estas unidades incluyen la Formación Estancia 25 de Mayo y la parte basal de la Formación Santa Cruz. La correlación litológica es, además, confirmada por datación de circones detríticos (edad máxima de 18,23±0,26 Ma) y un variado ensamble de vertebrados fósiles terrestres de edad post-Colhuehuapense a pre-Santacrucense en la escala de Edades Mamífero Sudamericanas (EMAS), con un rango temporal de entre 19 a 17,8 Ma. Edades similares han sido reportadas para la parte basal de la Formación Santa Cruz, en estancia Quién Sabe, en el suroeste de Argentina, ratificando la continuidad regional entre estos depósitos. Se presenta una columna estratigráfica y se interpreta el ambiente de depositación como una llanura costera con pequeños ríos sinuosos y lagos efímeros. La edad de sedimentación coincide con el solevantamiento de los Andes Patagónicos durante la 'Fase Quechua', lo que se ve reflejado por un cambio en la dirección de las paleocorrientes desde el noroeste hacia el este-noreste.http://ref.scielo.org/csxwd

The Gondwana connections of northern Patagonia

A multidisciplinary study (U–Pb sensitive high-resolution ion microprobe geochronology, Hf and O isotopes in zircon, Sr and Nd isotopes in whole-rocks, as well as major and trace element geochemistry) has been carried out on granitoid samples from the area west of Valcheta, North Patagonian Massif, Argentina. These confirm the Cambrian age of the Tardugno Granodiorite (528 ± 4 Ma) and the Late Permian age of granites in the central part of the Yaminué complex (250 Ma). Together with petrological and structural information for the area, we consider a previously suggested idea that the Cambrian and Ordovician granites of northeastern Patagonia represent continuation of the Pampean and Famatinian orogenic belts of the Sierras Pampeanas, respectively. Our interpretation does not support the hypothesis that Patagonia was accreted in Late Palaeozoic times as a far-travelled terrane, originating in the Central Transantarctic Mountains, and the arguments for and against this idea are reviewed. A parautochthonous origin is preferred with no major ocean closure between the North Patagonian Massif and the Sierra de la Ventana fold belt.Centro de Investigaciones GeológicasConsejo Nacional de Investigaciones Científicas y Técnica

Tracking of Salmonella Positive Pigs from Farm to Fork in the Republic of Ireland

In this study, individual pigs from selected herds of known Salmonella serological status were tracked through the slaughter and dressing process. From all tracked animals, caecal contents, rectal faeces, carcasses (before washing and chilling and after chilling) and pork primal cuts were examined for the presence of Salmonella. All samples were screened for Salmonella using real time PCR and all suspect positive samples were confirmed using the ISO 6579 method for Salmonella. To determine the relationship between Salmonella isolates from different parts of the chain , all isolates are being characterised by Pulse Field Gel Electrophoresis (PFGE). The results suggest that the slaughter and dressing operations have a significant effect on the incidence of Salmonella and that even if pigs are presented for slaughter with caecal or rectal carriage of Salmonella then good slaughter practices can prevent carcass contamination. All data generated in the study is being fed into a quantitative risk assessment model for Salmonella in pork

Detection of Escherichia coli O157 by Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) and Comparison to a Standard Culture Method

Despite the emergence of non-O157 shiga toxin-producing Escherichia coli (STEC) infections, E. coli O157 serotype is still the most commonly identified STEC in world. It causes high morbidity and mortality, and has been responsible for a number of outbreaks in many parts of the world. Various methods have been developed to detect this particular serotype, but standard bacteriological methods remain as the gold standard.In here, we propose a new peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the rapid detection of E. coli O157.Testing on 54 representative strains showed that the PNA probe is highly sensitive and specific to E. coli O157. Then, the method was optimized for the detection in food samples. Ground beef and unpasteurized milk samples were artificially contaminated with E. coli O157 concentrations ranging from 1×10-2 to 1×102 CFU per 25g or ml of food. Samples were then pre-enriched and analyzed by both the traditional bacteriological method (ISO 16654:2001) and PNA-FISH. The PNA-FISH method performed well in both types of food matrixes with a detection limit of 1 CFU/25 g or ml of food samples. Tests on 60 food samples have shown a specificity value of 100% (95% CI 82.83 - 100), a sensitivity of 97.22% (95% CI, 83.79 - 99.85) and an accuracy of 98,33% (CI 95%, 83.41 - 99.91). Results indicate that PNA-FISH performed as well as the traditional culture methods and can reduce the diagnosis time to 1 day.This work was supported by the Portuguese Institute Fundacao para a Ciencia e Tecnologia (FCT), project PIC/IC/82815/2007. C.A. acknowledges FCT for individual postdoctoral fellowship SFRH/BPD/74480/2010. We also acknowledge Biomode S.A. for providing some supplies for this project

First magmatism in the New England Batholith, Australia: forearc and arc–back-arc components in the Bakers Creek Suite gabbros

The New England Orogen, eastern Australia, was established as an outboard extension of the Lachlan Orogen through the migration of magmatism into forearc basin and accretionary prism sediments. Widespread S-type granitic rocks of the Hillgrove and Bundarra supersuites represent the first pulse of magmatism, followed by I- and A-types typical of circum-Pacific extensional accretionary orogens. Associated with the former are a number of small tholeiite–gabbroic to intermediate bodies of the Bakers Creek Suite, which sample the heat source for production of granitic magmas and are potential tectonic markers indicating why magmatism moved into the forearc and accretionary complexes rather than rifting the old Lachlan Orogen arc. The Bakers Creek Suite gabbros capture an early ( ∼  305 Ma) forearc basalt-like component with low Th ∕ Nb and with high Y ∕ Zr and Ba ∕ La, recording melting in the mantle wedge with little involvement of a slab flux and indicating forearc rifting. Subsequently, arc–back-arc like gabbroic magmas (305–304 Ma) were emplaced, followed by compositionally diverse magmatism leading up to the main S-type granitic intrusion ( ∼  290 Ma). This trend in magmatic evolution implicates forearc and other mantle wedge melts in the heating and melting of fertile accretion complex sediments and relatively long ( ∼  10 Myr) timescales for such melting

A history of Proterozoic terranes in southern South America: From Rodinia to Gondwana

The role played by Paleoproterozoic cratons in southern South America from the Mesoproterozoic to the Early Cambrian is reconsidered here. This period involved protracted continental amalgamation that led to formation of the supercontinent Rodinia, followed by Neoproterozoic continental break-up, with the consequent opening of Clymene and Iapetus oceans, and finally continental re-assembly as Gondwana through complex oblique collisions in the Late Neoproterozoic to Early Cambrian. The evidence for this is based mainly on a combination of precise U-Pb SHRMP dating and radiogenic isotope data for igneous and metamorphic rocks from a large area extending from the Rio de la Plata craton in the east to the Argentine Precordillera in the west and as far north as Arequipa in Peru. Our interpretation of the paleogeographical and geodynamic evolution invokes a hypothetical Paleoproterozoic block (MARA) embracing basement ultimately older than 1.7 Ga in the Western Sierras Pampeanas (Argentina), the Arequipa block (Peru), the Rio Apa block (Brazil), and probably also the Paraguaia block (Bolivia).Centro de Investigaciones Geológica

Divergent and convergent evolution after a common-source outbreak of hepatitis C virus.

The genomic sequences of viruses that are highly mutable and cause chronic infection tend to diverge over time. We report that these changes represent both immune-driven selection and, in the absence of immune pressure, reversion toward an ancestral consensus. Sequence changes in hepatitis C virus (HCV) structural and nonstructural genes were studied in a cohort of women accidentally infected with HCV in a rare common-source outbreak. We compared sequences present in serum obtained 18–22 yr after infection to sequences present in the shared inoculum and found that HCV evolved along a distinct path in each woman. Amino acid substitutions in known epitopes were directed away from consensus in persons having the HLA allele associated with that epitope (immune selection), and toward consensus in those lacking the allele (reversion). These data suggest that vaccines for genetically diverse viruses may be more effective if they represent consensus sequence, rather than a human isolate