LUCA ROSSETTI E BERNARDO ANTONIO VITTONE: MODELOS DE REFERÊNCIA E TRATADOS NA PINTURA EM QUADRATURA DO PIEMONTE

eoi/doi Deposit-Electronic Object Identifier   http://eoi.citefactor.org/10.11248/ehum.v13i2.3091 Luca Rossetti e Bernardo Antonio Vittone: modelli di riferimento e trattatistica nella pittura di quadratura in Piemonte Resumo: Luca Rossetti (1708-1770), originário de Orta e ativo entre a área de Cusio e os territórios do Ducado de Sabóia (Novara, Canavese e Biellese, hoje Piemonte), é conhecido na historiografia piemontesa e lombarda como pintor de figuras, ligada a modelos classicistas de derivação romano-bolonhesa. No entanto, em sua vasta produção de afrescos, contribuições importantes também são detectáveis no setor da quadratura. A contribuição pretende investigar os casos mais significativos e documentados em consideração à possível atividade de colaboração do artista com especialistas em arquitetura falsa e modelos de referência neste setor. Um primeiro exame das obras revelou surpreendentes citações da arquitetura, em particular de altares, de Bernardo Antonio Vittone (1704-1770), uma personalidade muito significativa para a história da arquitetura e dos tratados em meados do século XVIII, bem além das fronteiras do Reino da Sardenha em que a sua atividade está documentada. Por ocasião da publicação de Bernardo Antonio Vittone, pintura e pintores, em W. Canavesio (editado por), O gênio voluptuoso do olho. Novos estudos sobre Bernardo Antonio Vittone, Torino, Sociedade Piemontesa de Arqueologia e Belas Artes, 2005 mostraram claramente o interesse do arquiteto e a forte relação com os pintores quadraturistas a quem esta intervenção pretende fornecer mais elementos de reflexão.Palavra-chaves: Luca Rossetti, Piedmont art sec. XVIII, Bernardo Antonio Vittone.temática; Barcelona;Astratto: Luca Rossetti (1708-1770), originario di Orta e attivo tra l’area del Cusio e i territori del ducato di Savoia (novarese, canavese e biellese, oggi Piemonte), è noto alla storiografia piemontese e lombarda come pittore di figura, legato a modelli classicisti di derivazione romano-bolognese. Tuttavia, nella sua vasta produzione ad affresco sono rilevabili importanti contributi anche nel settore della quadratura. Il contributo intende indagare i casi più significativi e documentati in considerazione alla possibile attività di collaborazione dell’artista con specialisti in finte architetture e ai modelli di riferimento in questo settore. Una prima disamina delle opere ha rivelato sorprendenti citazioni dalle architetture, in particolare di altari, di Bernardo Antonio Vittone (1704-1770), personalità assai significativa per la storia dell’architettura e della trattatistica alla metà del XVIII secolo ben al di fuori dei confini del Regno di Sardegna in cui è documentata la sua attività. In occasione della pubblicazione Bernardo Antonio Vittone, la pittura ed i pittori, in W. Canavesio (a cura di), Il voluttuoso genio dell’occhio. Nuovi studi su Bernardo Antonio Vittone, Torino, Società Piemontese di Archeologia e Belle Arti, 2005 è emerso con chiarezza l’interesse e il rapporto forte dell’architetto con i pittori quadraturisti ai quali si intende fornire con il presente intervento ulteriori elementi di riflessione.Parole chiave: Luca Rossetti, Piemonte arte sec. XVIII, Bernardo Antonio Vittone.Recebido em: 10/07/2020 – Aceito em 31/07/202

A New ELISA Using the ANANAS Technology Showing High Sensitivity to diagnose the Bovine Rhinotracheitis from Individual Sera to Pooled Milk

Diagnostic tests for veterinary surveillance programs should be efficient, easy to use and, possibly, economical. In this context, classic Enzyme linked ImmunoSorbent Assay (ELISA) remains the most common analytical platform employed for serological analyses. The analysis of pooled samples instead of individual ones is a common procedure that permits to certify, with one single test, entire herds as "disease-free". However, diagnostic tests for pooled samples need to be particularly sensitive, especially when the levels of disease markers are low, as in the case of anti-BoHV1 antibodies in milk as markers of Infectious Bovine Rhinotracheitis (IBR) disease. The avidin-nucleic-acid-nanoassembly (ANANAS) is a novel kind of signal amplification platform for immunodiagnostics based on colloidal poly-avidin nanoparticles that, using model analytes, was shown to strongly increase ELISA test performance as compared to monomeric avidin. Here, for the first time, we applied the ANANAS reagent integration in a real diagnostic context. The monoclonal 1G10 anti-bovine IgG1 antibody was biotinylated and integrated with the ANANAS reagents for indirect IBR diagnosis from pooled milk mimicking tank samples from herds with IBR prevalence between 1 to 8%. The sensitivity and specificity of the ANANAS integrated method was compared to that of a classic test based on the same 1G10 antibody directly linked to horseradish peroxidase, and a commercial IDEXX kit recently introduced in the market. ANANAS integration increased by 5-fold the sensitivity of the 1G10 mAb-based conventional ELISA without loosing specificity. When compared to the commercial kit, the 1G10-ANANAS integrated method was capable to detect the presence of anti-BHV1 antibodies from bulk milk of gE antibody positive animals with 2-fold higher sensitivity and similar specificity. The results demonstrate the potentials of this new amplification technology, which permits improving current classic ELISA sensitivity limits without the need for new hardware investments

Femtometer-resolved simultaneous measurement of multiple laser wavelengths in a speckle wavemeter

Funding: Leverhulme Trust (RPG-2017-197); EPSRC (EP/R004854/1, EP/P030017/1).Many areas of optical science require an accurate measurement of optical spectra. Devices based on laser speckle promise compact wavelength measurement, with attometer-level sensitivity demonstrated for single wavelength laser fields. The measurement of multimode spectra using this approach would be attractive, yet this is currently limited to picometer resolution. Here, we present a method to improve the resolution and precision of speckle-based multi-wavelength measurements. We measure multiple wavelengths simultaneously, in a device comprising a single 1m-long step-index multimode fiber and a fast camera. Independent wavelengths separated by as little as 1 fm are retrieved with 0.2 fm precision using Principal Component Analysis. The method offers a viable way to measure sparse spectra containing multiple individual lines and is likely to find application in the tracking of multiple lasers in fields such as portable quantum technologies and optical telecommunications.PostprintPeer reviewe

Pattern of domestic violence from 2011 to 2015 in Beira, Mozambique

Background: Violence against women represents a violation of a fundamental human right and is a significant cause of death and disability worldwide. In developing countries, this issue is particularly dramatic and in sub-Saharan Africa were reached 65% of women reporting domestic violence.Objective: In this study, we assessed the burden and pattern of domestic violence registered at Beira Central Hospital, Mozam- bique from 2011 to 2015.Methods: We performed a descriptive analysis of data collected at the CHB Legal Medicine Service.Results: In five years, are recorded a total amount of 1,491 admissions for domestic violence of which 1307 were females. About 80% of all female cases are represented by the 11-40 age range and, in almost 90% the aggressor was the current or past partner. More than 75% were cases of repeated violence and in more than 60% there were minors attending the phenomenon.Conclusion: It is crucial to act immediately and with a multi-disciplinary approach in order to fight domestic violence, especially against women due to its dramatic consequences as isolation, inability to work, loss of wages, lack of participation in regular activities and limited ability to care for themselves and their children.Keywords: Domestic violence, violence against women, sexual violence, physical violence

Melatonin and Vitamin D Orchestrate Adipose Derived Stem Cell Fate by Modulating Epigenetic Regulatory Genes

Melatonin, that regulates many physiological processes including circadian rhythms, is a molecule able to promote osteoblasts maturation in vitro and to prevent bone loss in vivo, while regulating also adipocytes metabolism. In this regard, we have previously shown that melatonin in combination with vitamin D, is able to counteract the appearance of an adipogenic phenotype in adipose derived stem cells (ADSCs), cultured in an adipogenic favoring condition. In the present study, we aimed at evaluating the specific phenotype elicited by melatonin and vitamin D based medium, considering also the involvement of epigenetic regulating genes. ADSCs were cultured in a specific adipogenic conditioned media, in the presence of melatonin alone or with vitamin D. The expression of specific osteogenic related genes was evaluated at different time points, together with the histone deacetylases epigenetic regulators, HDAC1 and Sirtuins (SIRT) 1 and 2. Our results show that melatonin and vitamin D are able to modulate ADSCs commitment towards osteogenic phenotype through the upregulation of HDAC1, SIRT 1 and 2, unfolding an epigenetic regulation in stem cell differentiation and opening novel strategies for future therapeutic balancing of stem cell fate toward adipogenic or osteogenic phenotype

Molecular basis of mucopolysaccharidosis IVA (Morquio A syndrome) : a review and classification of GALNS gene variants and reporting of 68 novel variants

Mucopolysaccharidosis IVA (MPS IVA, Morquio A syndrome) is a rare autosomal recessive lysosomal storage disorder caused by mutations in the N-acetylgalactosamine-6-sulfatase (GALNS) gene. We collected, analyzed, and uniformly summarized all published GALNS gene variants, thus updating the previous mutation review (published in 2014). In addition, new variants were communicated by seven reference laboratories in Europe, the Middle East, Latin America, Asia, and the United States. All data were analyzed to determine common alleles, geographic distribution, level of homozygosity, and genotype-phenotype correlation. Moreover, variants were classified according to their pathogenicity as suggested by ACMG. Including those previously published, we assembled 446 unique variants, among which 68 were novel, from 1190 subjects (including newborn screening positive subjects). Variants' distribution was missense (65.0%), followed by nonsense (8.1%), splicing (7.2%), small frameshift deletions(del)/insertions(ins) (7.0%), intronic (4.0%), and large del/ins and complex rearrangements (3.8%). Half (50.4%) of the subjects were homozygous, 37.1% were compound heterozygous, and 10.7% had only one variant detected. The novel variants underwent in silico analysis to evaluate their pathogenicity. All variants were submitted to ClinVar (https://www.ncbi.nlm.nih.gov/clinvar/) to make them publicly available. Mutation updates are essential for the correct molecular diagnoses, genetic counseling, prenatal and preimplantation diagnosis, and disease management

Pattern of domestic violence from 2011 to 2015 in Beira, Mozambique

Background: Violence against women represents a violation of a fundamental human right and is a significant cause of death and disability worldwide. In developing countries, this issue is particularly dramatic and in sub-Saharan Africa were reached 65% of women reporting domestic violence. Objective: In this study, we assessed the burden and pattern of domestic violence registered at Beira Central Hospital, Mozambique from 2011 to 2015. Methods: We performed a descriptive analysis of data collected at the CHB Legal Medicine Service. Results: In five years, are recorded a total amount of 1,491 admissions for domestic violence of which 1307 were females. About 80% of all female cases are represented by the 11-40 age range and, in almost 90% the aggressor was the current or past partner. More than 75% were cases of repeated violence and in more than 60% there were minors attending the phenomenon. Conclusion: It is crucial to act immediately and with a multi-disciplinary approach in order to fight domestic violence, especially against women due to its dramatic consequences as isolation, inability to work, loss of wages, lack of participation in regular activities and limited ability to care for themselves and their children. DOI: https://dx.doi.org/10.4314/ahs.v19i1.23 Cite as: Cebola BR, Menegazzo F, Salmaso L, Facchin P, Isidoris V, Figueredo RL, et al. Pattern of domestic violence from 2011 to 2015 in Beira, Mozambique. Afri Health Sci. 2019;19(1). 1499-1506. https://dx.doi.org/10.4314/ahs.v19i1.2

Population medical genetics : translating science to the community

Rare genetic disorders are currently in the spotlight due to the elevated number of different conditions and significant total number of affected patients. The study of these disorders is extremely helpful for the elucidation of physiological processes related with complex disorders. Isolated populations are instrumental for the study of genetic disorders, considering their homogeneity and high proportion of affected patients in a small geographic area. These favorable conditions lead to the creation of a new discipline, known as "population medical genetics", which integrates medical genetics, population genetics, epidemiological genetics and community genetics. In order to develop practical activities in this new discipline, the National Institute of Population Medical Genetics (INaGeMP) was created in 2008 in Brazil. INaGeMP has developed several tools and funded numerous research activities. In this review, we highlight three successful projects developed in the first 10 years of INaGeMP activities (2008-2018): a newborn screening pilot study for MPS VI in Northeast Brazil, the study of Machado-Joseph disease in Brazilian families with Azorian ancestry, and the high twinning rate in a small town in southern Brazil. The results of these projects in terms of scientific output and contributions to the affected communities highlight the success and importance of INaGeMP