A diagnostic study comparing conventional and real-time PCR for Strongyloides stercoralis on urine and on faecal samples

Abstract Strongyloides stercoralis is a soil-transmitted helminth with a wide distribution in tropical and subtropical areas. The diagnosis of S. stercoralisinfection can be challenging, due to the low sensitivity of microscopic examination of stool samples and coproculture. In the last decade, different in-house molecular biology techniques for S. stercoralis have been implemented. They demonstrated good accuracy, although sensitivity does not seem sufficiently high yet. Recently, a novel PCR technique has been evaluated for the detection of S. stercoralis DNA in urine. Aim of this work was to compare the sensitivity of the real-time PCR (qPCR) on feces routinely used at the Centre for Tropical Disease (CTD) of Negrar, Verona, Italy, with that of the novel based PCR on urine. As secondary objective, we evaluated a Urine Conditioning Buffer ® (Zymoresearch) with the aim of improving nucleic acid stability in urine during sample storage/transport at ambient temperatures. Patients attending the CTD and resulting positive at routine screening with serology for S. stercoralis were invited, previous written consent, to supply stool and urine samples for molecular biology. A convenience sample of 30 patients was included. The sensitivity of qPCR on feces resulted 63%, and that of based PCR on urine was 17%. In all the samples treated with the Urine Conditioning Buffer ® there was no detectable DNA. In conclusion, the sensitivity of the novel technique resulted low, and needs further implementation before being considered as a valid alternative to the validated method

Effects of Impact and Target Parameters on the Results of a Kinetic Impactor: Predictions for the Double Asteroid Redirection Test (DART) Mission

The Double Asteroid Redirection Test (DART) spacecraft will impact into the asteroid Dimorphos on 2022 September 26 as a test of the kinetic impactor technique for planetary defense. The efficiency of the deflection following a kinetic impactor can be represented using the momentum enhancement factor, β, which is dependent on factors such as impact geometry and the specific target material properties. Currently, very little is known about Dimorphos and its material properties, which introduces uncertainty in the results of the deflection efficiency observables, including crater formation, ejecta distribution, and β. The DART Impact Modeling Working Group (IWG) is responsible for using impact simulations to better understand the results of the DART impact. Pre-impact simulation studies also provide considerable insight into how different properties and impact scenarios affect momentum enhancement following a kinetic impact. This insight provides a basis for predicting the effects of the DART impact and the first understanding of how to interpret results following the encounter. Following the DART impact, the knowledge gained from these studies will inform the initial simulations that will recreate the impact conditions, including providing estimates for potential material properties of Dimorphos and β resulting from DART’s impact. This paper summarizes, at a high level, what has been learned from the IWG simulations and experiments in preparation for the DART impact. While unknown, estimates for reasonable potential material properties of Dimorphos provide predictions for β of 1–5, depending on end-member cases in the strength regime

After DART: Using the First Full-scale Test of a Kinetic Impactor to Inform a Future Planetary Defense Mission

NASA’s Double Asteroid Redirection Test (DART) is the first full-scale test of an asteroid deflection technology. Results from the hypervelocity kinetic impact and Earth-based observations, coupled with LICIACube and the later Hera mission, will result in measurement of the momentum transfer efficiency accurate to ∼10% and characterization of the Didymos binary system. But DART is a single experiment; how could these results be used in a future planetary defense necessity involving a different asteroid? We examine what aspects of Dimorphos’s response to kinetic impact will be constrained by DART results; how these constraints will help refine knowledge of the physical properties of asteroidal materials and predictive power of impact simulations; what information about a potential Earth impactor could be acquired before a deflection effort; and how design of a deflection mission should be informed by this understanding. We generalize the momentum enhancement factor β, showing that a particular direction-specific β will be directly determined by the DART results, and that a related direction-specific β is a figure of merit for a kinetic impact mission. The DART β determination constrains the ejecta momentum vector, which, with hydrodynamic simulations, constrains the physical properties of Dimorphos’s near-surface. In a hypothetical planetary defense exigency, extrapolating these constraints to a newly discovered asteroid will require Earth-based observations and benefit from in situ reconnaissance. We show representative predictions for momentum transfer based on different levels of reconnaissance and discuss strategic targeting to optimize the deflection and reduce the risk of a counterproductive deflection in the wrong direction

The Dimorphos ejecta plume properties revealed by LICIACube

The Double Asteroid Redirection Test (DART) had an impact with Dimorphos (a satellite of the asteroid Didymos) on 26 September 20221. Ground-based observations showed that the Didymos system brightened by a factor of 8.3 after the impact because of ejecta, returning to the pre-impact brightness 23.7 days afterwards2. Hubble Space Telescope observations made from 15 minutes after impact to 18.5 days after, with a spatial resolution of 2.1 kilometres per pixel, showed a complex evolution of the ejecta3, consistent with other asteroid impact events. The momentum enhancement factor, determined using the measured binary period change4, ranges between 2.2 and 4.9, depending on the assumptions about the mass and density of Dimorphos5. Here we report observations from the LUKE and LEIA instruments on the LICIACube cube satellite, which was deployed 15 days in advance of the impact of DART. Data were taken from 71 seconds before the impact until 320 seconds afterwards. The ejecta plume was a cone with an aperture angle of 140 ± 4 degrees. The inner region of the plume was blue, becoming redder with increasing distance from Dimorphos. The ejecta plume exhibited a complex and inhomogeneous structure, characterized by filaments, dust grains and single or clustered boulders. The ejecta velocities ranged from a few tens of metres per second to about 500 metres per second.This work was supported by the Italian Space Agency (ASI) in the LICIACube project (ASI-INAF agreement AC no. 2019-31-HH.0) and by the DART mission, NASA contract 80MSFC20D0004. M.Z. acknowledges Caltech and the Jet Propulsion Laboratory for granting the University of Bologna a licence to an executable version of MONTE Project Edition software. M.Z. is grateful to D. Lubey, M. Smith, D. Mages, C. Hollenberg and S. Bhaskaran of NASA/JPL for the discussions and suggestions regarding the operational navigation of LICIACube. G.P. acknowledges financial support from the Centre national d’études spatiales (CNES, France). A.C.B. acknowledges funding by the NEO-MAPP project (grant agreement 870377, EC H2020-SPACE-2019) and by the Ministerio de Ciencia Innovación (PGC 2018) RTI2018-099464-B-I00. F.F. acknowledges funding from the Swiss National Science Foundation (SNSF) Ambizione (grant no. 193346). J.-Y.L. acknowledges the support from the NASA DART Participating Scientist Program (grant no. 80NSSC21K1131). S.D.R. and M.J. acknowledge support from the Swiss National Science Foundation (project no. 200021_207359)

After DART: Using the first full-scale test of a kinetic impactor to inform a future planetary defense mission

NASA's Double Asteroid Redirection Test (DART) is the first full-scale test of an asteroid deflection technology. Results from the hypervelocity kinetic impact and Earth-based observations, coupled with LICIACube and the later Hera mission, will result in measurement of the momentum transfer efficiency accurate to ~10% and characterization of the Didymos binary system. But DART is a single experiment; how could these results be used in a future planetary defense necessity involving a different asteroid? We examine what aspects of Dimorphos's response to kinetic impact will be constrained by DART results; how these constraints will help refine knowledge of the physical properties of asteroidal materials and predictive power of impact simulations; what information about a potential Earth impactor could be acquired before a deflection effort; and how design of a deflection mission should be informed by this understanding. We generalize the momentum enhancement factor $\beta$, showing that a particular direction-specific $\beta$ will be directly determined by the DART results, and that a related direction-specific $\beta$ is a figure of merit for a kinetic impact mission. The DART $\beta$ determination constrains the ejecta momentum vector, which, with hydrodynamic simulations, constrains the physical properties of Dimorphos's near-surface. In a hypothetical planetary defense exigency, extrapolating these constraints to a newly discovered asteroid will require Earth-based observations and benefit from in-situ reconnaissance. We show representative predictions for momentum transfer based on different levels of reconnaissance and discuss strategic targeting to optimize the deflection and reduce the risk of a counterproductive deflection in the wrong direction

After DART: Using the First Full-scale Test of a Kinetic Impactor to Inform a Future Planetary Defense Mission

After DART: Using the First Full-scale Test of a Kinetic Impactor to Inform a Future Planetary Defense Mission Thomas S. Statler 1 , Sabina D. Raducan 2 , Olivier S. Barnouin 3 , Mallory E. DeCoster 3 , Steven R. Chesley 4 , Brent Barbee 5 , Harrison F. Agrusa 6 , Saverio Cambioni 7 , Andrew F. Cheng 3 , Elisabetta Dotto 8 , Siegfried Eggl9 , Eugene G. Fahnestock 4 , Fabio Ferrari 2 , Dawn Graninger 3 , Alain Herique 10 , Isabel Herreros 11 , Masatoshi Hirabayashi 12,13 , Stavro Ivanovski 14 , Martin Jutzi 2 , Özgür Karatekin 15 , Alice Lucchetti 16 , Robert Luther 17 , Rahil Makadia 9 , Francesco Marzari 18 , Patrick Michel 19 , Naomi Murdoch 20 , Ryota Nakano13 , Jens Ormö 11 , Maurizio Pajola 16 , Andrew S. Rivkin3 , Alessandro Rossi 21 , Paul Sánchez 22 , Stephen R. Schwartz 23 , Stefania Soldini 24 , Damya Souami 19 , Angela Stickle 3 , Paolo Tortora 25 , Josep M. Trigo-Rodríguez 26,27 , Flaviane Venditti 28 , Jean-Baptiste Vincent 29 , and Kai Wünnemann 17,30 1 Planetary Defense Coordination Office and Planetary Science Division, NASA Headquarters, 300 Hidden Figures Way SW, Washington, DC 20546, USA [email protected] 2 Space Research and Planetary Sciences, Physics Institute, University of Bern, Bern, 3012, Switzerland 3 Johns Hopkins University Applied Physics Laboratory, Laurel, MD 20723, USA 4 Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA 5 NASA Goddard Space Flight Center, Greenbelt, MD 20771, USA 6 Department of Astronomy, University of Maryland, College Park, MD 20742, USA 7 Department of Earth, Atmospheric & Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA 8 INAF-Osservatorio Astronomico di Roma, Rome, I-00078, Italy 9 Department of Aerospace Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 10 Univ. Grenoble Alpes, CNRS, CNES, IPAG, F-38000 Grenoble, France 11 Centro de Astrobiología CSIC-INTA, Instituto Nacional de Técnica Aeroespacial, E-28850 Torrejón de Ardoz, Spain 12 Department of Geosciences, Auburn University, Auburn, AL 36849, USA 13 Department of Aerospace Engineering, Auburn University, Auburn, AL 36849, USA 14 INAF- Osservatorio Astronomico di Trieste, Trieste I-34143, Italy 15 Royal Observatory of Belgium, Belgium 16 INAF-Astronomical Observatory of Padova, Padova I-35122, Italy 17 Museum für Naturkunde—Leibniz Institute for Evolution and Biodiversity Science, Germany 18 University of Padova, Padova, Italy 19 Université Côte d’Azur, Observatoire de la Côte d’Azur, CNRS, Laboratoire Lagrange, Nice F-06304, France 20 Institut Supérieur de l’Aéronautique et de l’Espace (ISAE-SUPAERO), Université de Toulouse, Toulouse, France 21 IFAC-CNR, Sesto Fiorentino I-50019, Italy 22 Colorado Center for Astrodynamics Research, University of Colorado Boulder, Boulder, CO 80303, USA 23 Planetary Science Institute, Tucson, AZ 85719, USA 24 Department of Mechanical, Materials and Aerospace Engineering, University of Liverpool, Liverpool, UK 25 Alma Mater Studiorum—Università di Bologna, Department of Industrial Engineering, Interdepartmental Center for Industrial Research in Aerospace, Via Fontanelle 40—Forlì (FC)—I-47121, Italy 26 Institute of Space Sciences (ICE, CSIC), Cerdanyola del Vallès, E-08193 Barcelona, Catalonia, Spain 27 Institut d’Estudis Espacials de Catalunya (IEEC), Ed. Nexus, E-08034 Barcelona, Catalonia, Spain 28 Arecibo Observatory, University of Central Florida, HC-3 Box 53995, Arecibo, PR 00612, USA 29 German Aerospace Center, DLR Berlin, Germany 30 Freie Universität Berlin, Germany Received 2022 August 9; revised 2022 September 18; accepted 2022 September 22; published 2022 October 28 Abstract NASA’s Double Asteroid Redirection Test (DART) is the first full-scale test of an asteroid deflection technology. Results from the hypervelocity kinetic impact and Earth-based observations, coupled with LICIACube and the later Hera mission, will result in measurement of the momentum transfer efficiency accurate to ∼10% and characterization of the Didymos binary system. But DART is a single experiment; how could these results be used in a future planetary defense necessity involving a different asteroid? We examine what aspects of Dimorphos’s response to kinetic impact will be constrained by DART results; how these constraints will help refine knowledge of the physical properties of asteroidal materials and predictive power of impact simulations; what information about a potential Earth impactor could be acquired before a deflection effort; and how design of a deflection mission should be informed by this understanding. We generalize the momentum enhancement factor β, showing that a particular direction-specific β will be directly determined by the DART results, and that a related direction- specific β is a figure of merit for a kinetic impact mission. The DART β determination constrains the ejecta momentum vector, which, with hydrodynamic simulations, constrains the physical properties of Dimorphos’s near- surface. In a hypothetical planetary defense exigency, extrapolating these constraints to a newly discovered asteroid will require Earth-based observations and benefit from in situ reconnaissance. We show representative predictions for momentum transfer based on different levels of reconnaissance and discuss strategic targeting to optimize the deflection and reduce the risk of a counterproductive deflection in the wrong direction

Achievement of the planetary defense investigations of the Double Asteroid Redirection Test (DART) mission

NASA's Double Asteroid Redirection Test (DART) mission was the first to demonstrate asteroid deflection, and the mission's Level 1 requirements guided its planetary defense investigations. Here, we summarize DART's achievement of those requirements. On 2022 September 26, the DART spacecraft impacted Dimorphos, the secondary member of the Didymos near-Earth asteroid binary system, demonstrating an autonomously navigated kinetic impact into an asteroid with limited prior knowledge for planetary defense. Months of subsequent Earth-based observations showed that the binary orbital period was changed by –33.24 minutes, with two independent analysis methods each reporting a 1σ uncertainty of 1.4 s. Dynamical models determined that the momentum enhancement factor, β, resulting from DART's kinetic impact test is between 2.4 and 4.9, depending on the mass of Dimorphos, which remains the largest source of uncertainty. Over five dozen telescopes across the globe and in space, along with the Light Italian CubeSat for Imaging of Asteroids, have contributed to DART's investigations. These combined investigations have addressed topics related to the ejecta, dynamics, impact event, and properties of both asteroids in the binary system. A year following DART's successful impact into Dimorphos, the mission has achieved its planetary defense requirements, although work to further understand DART's kinetic impact test and the Didymos system will continue. In particular, ESA's Hera mission is planned to perform extensive measurements in 2027 during its rendezvous with the Didymos–Dimorphos system, building on DART to advance our knowledge and continue the ongoing international collaboration for planetary defense

Preliminary comparison of an in-house real-time PCR with the automated BD Max Enteric Parasite Panel for the detection of Giardia intestinalis

Giardia intestinalis is a parasite that commonly causes diarrheal disease throughout the world. An accurate and rapid diagnosis is essential to reduce the infection. Classically, diagnosis of giardiasis is performed by microscopic examination of stool samples, but in the recent years many DNA-based methods have been developed. In this preliminary observational study we compared the results of the commercial BD Max Enteric Parasite Panel (EPP) with an in-house real-time PCR for Giardia intestinalis. The study population was composed by 73 samples; of these, 27 resulted positive at both techniques and 39 negative. Seven samples were positive at the in-house real-time PCR and negative at the BD Max EPP. The Cohen's Kappa resulted 0.805 (95% CI 0.670-0.940). In conclusion, these preliminary results suggest that the Rt-PCR could possibly demonstrate higher sensitivity for the diagnosis of Giardia intestinalis than BD Max EPP, that tended to miss infection of low intensity

Molecular Biology Can Change the Classic Laboratory Approach for Intestinal Protozoan Infections

For many years microscopy has been considered the mainstay of the diagnosis of parasitic infections. In our laboratory, before the advent of molecular biology, the approach for the identification of parasitic infections in stools was the microscopic exam of three samples. Once we adopted molecular biology, a real-time PCR on one single sample was added to the classical coproparasitological exam of three samples. Given the high sensitivity of real-time PCR (Rt-PCR), we then decided to evaluate if a change of our routine was justified. In detail, we intended to assess if a much more practical routine, based on the analysis of a single fecal sample, was sufficiently sensitive to replace the routine described above. The new approach to be evaluated included, on the same and unique fecal sample, a classical coproparasitological exam plus Rt-PCR. The data obtained showed that the sensitivity of the new proposed approach remains very high, despite the reduction of coproparasitological exams from three to one, with the advantage of reducing costs and saving time, both for patients and for the laboratory

Preliminary comparison between an in-house real-time PCR vs microscopy for the diagnosis of Loa loa and Mansonella perstans

Infections with the filarial nematodes Loa loa and Mansonella perstans are among the most neglected filarial infections. L. loa is endemic in 11 countries of Central and West Africa and loiasis is estimated to affect about 20 million people. M. perstans infection is widespread in more than 30 countries of sub-Saharan Africa. Due to the difficulty in diagnosing loiasis and M. perstans mansonellosis on a clinical basis, the diagnosis of infection with L. loa and M. perstans relies on laboratory techniques. Definitive diagnosis is based on the detection, identification, and quantification of circulating microfilariae (mf) by microscopy of concentrated blood. However, this is impractical for screening purposes as it requires expert laboratory personnel, considerable blood manipulation, and is time consuming, especially for the final issue of negative result reports, which are very common in the population visited outside endemic areas. The aim of the current work is the preliminary evaluation of the performance of the in-house real-time PCR described by Ta and colleagues compared to the routine microscopic approach for the screening of filarial infections in the clinical setting outside endemic areas, using samples from patients accessing the dedicated outpatient clinics for migrants and travelers of a reference centre for tropical diseases in Northern Italy