334 research outputs found
Adult demography and larval processes in coastal benthic populations intertidal barnacles in Southern California and Baja California
Submitted in partial fulfillment of the requirements for the degree of
Doctor of Philosophy
at the
Massachusetts Institute of Technology
and the
Woods Hole Oceanographic Institution
September 2005The geographic distribution and dynamics of coastal benthic populations are shaped by physical biological
interactions affecting larval dispersal and the demography of juvenile and adult
individuals. This thesis focused on nearshore patterns of larval distribution and regional patterns
in demography of intertidal barnacles in Southern and Baja California. Horizontal and vertical
distributions, and the mortality rates of larvae, were assessed from short tenn (i.e. days) smallscale
observations (0.1-1 km) in nearshore waters. Observations on spatial variability of adult
barnacle demography were gathered over 1.5 years at scales of hundreds of kilometers.
Stage-specific horizontal distributions and nearshore current measurements suggested that larvae
of Balanus glandula and Chthamalus spp. may experience limited dispersal. High mortality rates
could further limit travel distances and the exchange of individuals among disjunct populations.
Data on vertical distributions indicated that nauplii and cyprids of Balanus nubilus and Pollicipes
polymerus occur at different depths. Nauplii remained near the surface at all times, whereas
cyprids occurred in the bottom half of the water column. Such distributions, combined with
vertical variability in horizontal flows, might cause the observed horizontal segregation ofnauplii
and cyprids.
Differences in survival, growth rate,· size structure, and per capita fertility of adult Balanus
glandula were observed between Dana Point (Southern California) and Punta Baja (Baja
California), a site located near the species' southern limit of distribution. Effects of spatial
differences in demography on population persistence were assessed with a stage-structured matrix
model. Model analyses indicated that the Punta Baja population is more susceptible to
environmental stochasticity and more prone to local extinction than populations located further
north.
This thesis emphasizes the importance of characterizing factors that affect the dynamics of
benthic populations at mUltiple spatial-temporal scales, and the usefulness of small· scale highfrequency
observations of nearshore phenomena, especially in relation with the dispersal of
larvae.I must thank the
Chilean MIDEPLAN (Ministerio de Plartificacion y Cooperacion), which provided funds
for the first three years of my doctoral studies through a Presidential Fellowship (Beca
Presidente de la Republica). Tuition, stipend, and research funds for the rest of my time.
as a Joint Program student carne from National Science Foundation grants OCE-0083976
and OCE-9986627 to my thesis supervisor (Jesus Pineda), and through the WHOI
Academic Programs Office
Förster Resonance Energy Transfer (FRET) Correlates of Altered Subunit Stoichiometry in Cys-Loop Receptors, Exemplified by Nicotinic α4β2
We provide a theory for employing Förster resonance energy transfer (FRET)
measurements to determine altered heteropentameric ion channel stoichiometries in
intracellular compartments of living cells. We simulate FRET within nicotinic receptors
(nAChRs) whose α4 and β2 subunits contain acceptor and donor fluorescent protein
moieties, respectively, within the cytoplasmic loops. We predict FRET and normalized
FRET (NFRET) for the two predominant stoichiometries, (α4)3(β2)2 vs. (α4)2(β2)3.
Studying the ratio between FRET or NFRET for the two stoichiometries, minimizes
distortions due to various photophysical uncertainties. Within a range of assumptions
concerning the distance between fluorophores, deviations from plane pentameric geometry,
and other asymmetries, the predicted FRET and NFRET for (α4)3(β2)2 exceeds that of
(α4)2(β2)3. The simulations account for published data on transfected Neuro2a cells in
which α4β2 stoichiometries were manipulated by varying fluorescent subunit cDNA ratios:
NFRET decreased monotonically from (α4)3(β2)2 stoichiometry to mostly (α4)2(β2)3. The
simulations also account for previous macroscopic and single-channel observations that
pharmacological chaperoning by nicotine and cytisine increase the (α4)2(β2)3 and
(α4)3(β2)2 populations, respectively. We also analyze sources of variability. NFRET-based monitoring of changes in subunit stoichiometry can contribute usefully to studies on
Cys-loop receptors
Chromosome-Level Genome Assembly of the Blue Mussel Mytilus chilensis Reveals Molecular Signatures Facing the Marine Environment
The blue mussel Mytilus chilensis is an endemic and key socioeconomic species inhabiting the southern coast of Chile. This bivalve species supports a booming aquaculture industry, which entirely relies on artificially collected seeds from natural beds that are translocated to diverse physical–chemical ocean farming conditions. Furthermore, mussel production is threatened by a broad range of microorganisms, pollution, and environmental stressors that eventually impact its survival and growth. Herein, understanding the genomic basis of the local adaption is pivotal to developing sustainable shellfish aquaculture. We present a high-quality reference genome of M. chilensis, which is the first chromosome-level genome for a Mytilidae member in South America. The assembled genome size was 1.93 Gb, with a contig N50 of 134 Mb. Through Hi-C proximity ligation, 11,868 contigs were clustered, ordered, and assembled into 14 chromosomes in congruence with the karyological evidence. The M. chilensis genome comprises 34,530 genes and 4795 non-coding RNAs. A total of 57% of the genome contains repetitive sequences with predominancy of LTR-retrotransposons and unknown elements. Comparative genome analysis of M. chilensis and M. coruscus was conducted, revealing genic rearrangements distributed into the whole genome. Notably, transposable Steamer-like elements associated with horizontal transmissible cancer were explored in reference genomes, suggesting putative relationships at the chromosome level in Bivalvia. Genome expression analysis was also conducted, showing putative genomic differences between two ecologically different mussel populations. The evidence suggests that local genome adaptation and physiological plasticity can be analyzed to develop sustainable mussel production. The genome of M. chilensis provides pivotal molecular knowledge for the Mytilus complex
Combined fit to the spectrum and composition data measured by the Pierre Auger Observatory including magnetic horizon effects
The measurements by the Pierre Auger Observatory of the energy spectrum and mass composition of cosmic rays can be interpreted assuming the presence of two extragalactic source populations, one dominating the flux at energies above a few EeV and the other below. To fit the data ignoring magnetic field effects, the high-energy population needs to accelerate a mixture of nuclei with very hard spectra, at odds with the approximate E shape expected from diffusive shock acceleration. The presence of turbulent extragalactic magnetic fields in the region between the closest sources and the Earth can significantly modify the observed CR spectrum with respect to that emitted by the sources, reducing the flux of low-rigidity particles that reach the Earth. We here take into account this magnetic horizon effect in the combined fit of the spectrum and shower depth distributions, exploring the possibility that a spectrum for the high-energy population sources with a shape closer to E be able to explain the observations
Studies of the mass composition of cosmic rays and proton-proton interaction cross-sections at ultra-high energies with the Pierre Auger Observatory
In this work, we present an estimate of the cosmic-ray mass composition from the distributions of the depth of the shower maximum (Xmax) measured by the fluorescence detector of the Pierre Auger Observatory. We discuss the sensitivity of the mass composition measurements to the uncertainties in the properties of the hadronic interactions, particularly in the predictions of the particle interaction cross-sections. For this purpose, we adjust the fractions of cosmic-ray mass groups to fit the data with Xmax distributions from air shower simulations. We modify the proton-proton cross-sections at ultra-high energies, and the corresponding air shower simulations with rescaled nucleus-air cross-sections are obtained via Glauber theory. We compare the energy-dependent composition of ultra-high-energy cosmic rays obtained for the different extrapolations of the proton-proton cross-sections from low-energy accelerator data
Study of downward Terrestrial Gamma-ray Flashes with the surface detector of the Pierre Auger Observatory
The surface detector (SD) of the Pierre Auger Observatory, consisting of 1660 water-Cherenkov detectors (WCDs), covers 3000 km2 in the Argentinian pampa. Thanks to the high efficiency of WCDs in detecting gamma rays, it represents a unique instrument for studying downward Terrestrial Gamma-ray Flashes (TGFs) over a large area. Peculiar events, likely related to downward TGFs, were detected at the Auger Observatory. Their experimental signature and time evolution are very different from those of a shower produced by an ultrahigh-energy cosmic ray. They happen in coincidence with low thunderclouds and lightning, and their large deposited energy at the ground is compatible with that of a standard downward TGF with the source a few kilometers above the ground. A new trigger algorithm to increase the TGF-like event statistics was installed in the whole array. The study of the performance of the new trigger system during the lightning season is ongoing and will provide a handle to develop improved algorithms to implement in the Auger upgraded electronic boards. The available data sample, even if small, can give important clues about the TGF production models, in particular, the shape of WCD signals. Moreover, the SD allows us to observe more than one point in the TGF beam, providing information on the emission angle
The dynamic range of the upgraded surface-detector stations of AugerPrime
The detection of ultra-high-energy cosmic rays by means of giant detector arrays is often limited by the saturation of the recorded signals near the impact point of the shower core at the ground, where the particle density dramatically increases. The saturation affects in particular the highest energy events, worsening the systematic uncertainties in the reconstruction of the shower characteristics. The upgrade of the Pierre Auger Observatory, called AugerPrime, includes the installation of an 1-inch Small PhotoMultiplier Tube (SPMT) inside each water-Cherenkov station (WCD) of the surface detector array. The SPMT allows an unambiguous measurement of signals down to about 250m from the shower core, thus reducing the number of events featuring a saturated station to a negligible level. In addition, a 3.8m2 plastic scintillator (Scintillator Surface Detector, SSD) is installed on top of each WCD. The SSD is designed to match the WCD (with SPMT) dynamic range, providing a complementary measurement of the shower components up to the highest energies. In this work, the design and performances of the upgraded AugerPrime surface-detector stations in the extended dynamic range are described, highlighting the accuracy of the measurements. A first analysis employing the unsaturated signals in the event reconstruction is also presented
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