Commentary: Nicotinic acetylcholine receptor α9 and α10 subunits are expressed in the brain of mice

Fil: Morley, Barbara J.. Boys Town National Research Hospita; Estados UnidosFil: Whiteaker, Paul. Barrow Neurological Institute; Estados UnidosFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentin

Federal Income Taxes and the Civil Fraud Penalty

The drive against tax evaders is now in full swing after a complete reorganization of the Internal Revenue Service, and the new Commissioner has promised an efficient enforcement of all revenue laws. Although the effective administration of the federal income tax rests primarily upon the willingness of the taxpayer voluntarily to disclose his correct income, Congress has provided certain civil and criminal penalties to punish those who have not fulfilled their obligations to the United States Treasury. This article will deal only with the administration and operation of the civil fraud penalty. The most severe civil penalty that may be inflicted, the 50 percent fraud penalty, is actually not regarded by the courts as a penalty at all, but has been described merely as a compensatory sum to reimburse the Government for its added expense in detecting the violation. Although the reasoning behind this result is not entirely convincing, it has served as the basis for imposing the fraud penalty against the estate of a taxpayer after his death

The Influence of Johann Wolfgang von Goethe on the Legacy of the Bauhaus

This paper addresses three color theorists of the Bauhaus and their connection to a single historical source, Johann Wolfgang von Goethe. It begins with an overview of the Bauhaus and a brief chronology of the school, and then moves into a description of Goethe’s work, primarily Theory of Color. I then discuss Wassily Kandinsky, his writings in Concerning the Spiritual in Art and From Point and Line to Plane, and his impact on the other teachers of the Bauhaus. The third chapter addresses Johannes Itten’s book. The Art of Color, and his influence on the Bauhaus and its students through his introductory course, the Vorkurs. The final chapter discusses Josef Albers, a Bauhaus student-turned-teacher and painter, who taught at Black Mountain College for over twenty years and wrote interaction of Color, I attempt to connect them using Goethe’s psychological and physiological color classifications, as well as establishing a physical link between them. I propose that Goethe’s focus on the beholder rather than the color itself carried through to the work of Kandinsky, Itten and Albers, and attempt to establish this through their writings as well as their work

A Machine Learning Approach to Jet-Surface Interaction Noise Modeling

This paper investigates using machine learning to rapidly develop empirical models suitable for system-level aircraft noise studies. In particular, machine learning is used to train a neural network to predict the noise spectra produced by a round jet near a surface over a range of surface lengths, surface standoff distances, jet Mach numbers, and observer angles. These spectra include two sources, jet-mixing noise and jet-surface interaction (JSI) noise, with different scale factors as well as surface shielding and reflection effects to create a multi- dimensional problem. A second model is then trained using data from three rectangular nozzles to include nozzle aspect ratio in the spectral prediction. The training and validation data are from an extensive jet-surface interaction noise database acquired at the NASA Glenn Research Center's Aero-Acoustic Propulsion Laboratory. Although the number of training and validation points is small compared a typical machine learning application, the results of this investigation show that this approach is viable if the underlying data are well behaved

Mechanism Of Allosteric Modulation Of The Cys-Loop Receptors

The cys-loop receptor family is a major family of neurotransmitter-operated ion channels. They play important roles in fast synaptic transmission, controlling neuronal excitability, and brain function. These receptors are allosteric proteins, in that binding of a neurotransmitter to its binding site remotely controls the channel function. The cys-loop receptors also are subject to allosteric modulation by many pharmaceutical agents and endogenous modulators. By binding to a site of the receptor distinct from the neurotransmitter binding site, allosteric modulators alter the response of the receptors to their agonists. The mechanism of allosteric modulation is traditionally believed to be that allosteric modulators directly change the binding affinity of receptors for their agonists. More recent studies support the notion that these allosteric modulators are very weak agonists or antagonists by themselves. They directly alter channel gating, and thus change the distribution of the receptor across multiple different affinity states, indirectly influencing receptors\u27 sensitivity to agonists. There are two major locations of allosteric modulator binding sites. One is in subunit interfaces of the amino-terminal domain. The other is in the transmembrane domain close to the channel gating machinery. In this review, we also give some examples of well characterized allosteric binding pockets. © 2010 by the authors

A prototype toolset for the ArcGIS Hydro Data Model

Center for Research in Water Resource

Distinctive Single-Channel Properties Of α4β2-Nicotinic Acetylcholine Receptor Isoforms

Central nervous system nicotinic acetylcholine receptors (nAChR) are predominantly of the α4β2 subtype. Two isoforms exist, with high or low agonist sensitivity (HS-(α4β2) 2 β2- and LS-(α4β2) 2 α4-nAChR). Both isoforms exhibit similar macroscopic potency and efficacy values at low acetylcholine (ACh) concentrations, mediated by a common pair of high-affinity α4(+)/(-)β2 subunit binding interfaces. However LS-(α4β2) 2 α4-nAChR also respond to higher concentrations of ACh, acting at a third α4(+)/(-)α4 subunit interface. To probe isoform functional differences further, HS- and LS-α4β2-nAChR were expressed in Xenopus laevis oocytes and single-channel responses were assessed using cell-attached patch-clamp. In the presence of a low ACh concentration, both isoforms produce low-bursting function. HS-(α4β2) 2 β2-nAChR exhibit a single conductance state, whereas LS-(α4β2) 2 α4-nAChR display two distinctive conductance states. A higher ACh concentration did not preferentially recruit either conductance state, but did result in increased LS-(α4β2) 2 α4-nAChR bursting and reduced closed times. Introduction of an α4(+)/(-)α4-interface loss-of-function α4W182A mutation abolished these changes, confirming this site’s role in mediating LS-(α4β2) 2 α4-nAChR responses. Small or large amplitude openings are highly-correlated within individual LS-(α4β2) 2 α4-nAChR bursts, suggesting that they arise from distinct intermediate states, each of which is stabilized by α4(+)/(-)α4 site ACh binding. These findings are consistent with α4(+)/(-)α4 subunit interface occupation resulting in allosteric potentiation of agonist actions at α4(+)/(-)β2 subunit interfaces, rather than independent induction of high conductance channel openings

LSST Classifier

The LSST (Large Synoptic Survey Telescope) Space Image Classifier Team built an application that uses crowd sourcing and machine learning to classify space objects in LSST image data. This project aims to work towards LSST science outreach goals.https://pilotscholars.up.edu/egr_project/1003/thumbnail.jp