Evolutionary constraints on the complexity of genetic regulatory networks allow predictions of the total number of genetic interactions

Genetic regulatory networks (GRNs) have been widely studied, yet there is a lack of understanding with regards to the final size and properties of these networks, mainly due to no network currently being complete. In this study, we analyzed the distribution of GRN structural properties across a large set of distinct prokaryotic organisms and found a set of constrained characteristics such as network density and number of regulators. Our results allowed us to estimate the number of interactions that complete networks would have, a valuable insight that could aid in the daunting task of network curation, prediction, and validation. Using state-of-the-art statistical approaches, we also provided new evidence to settle a previously stated controversy that raised the possibility of complete biological networks being random and therefore attributing the observed scale-free properties to an artifact emerging from the sampling process during network discovery. Furthermore, we identified a set of properties that enabled us to assess the consistency of the connectivity distribution for various GRNs against different alternative statistical distributions. Our results favor the hypothesis that highly connected nodes (hubs) are not a consequence of network incompleteness. Finally, an interaction coverage computed for the GRNs as a proxy for completeness revealed that high-throughput based reconstructions of GRNs could yield biased networks with a low average clustering coefficient, showing that classical targeted discovery of interactions is still needed.Comment: 28 pages, 5 figures, 12 pages supplementary informatio

"Nutritional or Hormonal", The Myth of Takotsubo Cardiomyopathy and Left Ventricular Thrombosis in Anorexia Nervosa Patients! A Case Report

Takotsubo Cardiomyopathy is a condition characterized by transient LV hypokinesis without evidence of obstructive coronary disease that is typically triggered by a stressful event. We describe a 34-year-old woman with anorexia nervosa presenting with hypotension, hypoglycemia, and tachycardia and found to have Takotsubo Cardiomyopathy. On admission, EKG showed ST depression with T wave inversion in the inferolateral leads and Q waves in the anteroseptal leads (V1-V3). The initial troponin was elevated at 1.2 ng/ml and increased to 1.94 ng/ml 4 hours later. Initial TTE showed global hypokinesisexcept for a hyperkinetic basal segment suggestive of takostsubo cardiomyopathy and an EF of 15-20%. During the course of management, subsequent TTEs revealed improved EF with resolution of the cardiomyopathy, but also the presence of an LV thrombus. The pathophysiology of Takotsubo Cardiomyopathy is not fully understood, and the impact of the hypoestrogenic state and the nutritional deficiencies on the development Takotsubo Cardiomyopathy in anorexia nervosa patients remains to be a myth

On Nichols algebras over SL(2,Fq) and GL(2,Fq)

We compute necessary conditions on Yetter-Drinfeld modules over the groups SL(2,Fq) and GL(2,Fq) to generate finite dimensional Nichols algebras. This is a first step towards a classification of pointed Hopf algebras with a group of group-likes isomorphic to one of these groups.Comment: Major exposition revision, including referees remarks. To appear in J. Math. Phys. 13 page

Corynebacterium glutamicum regulation beyond transcription: Organizing principles and reconstruction of an extended regulatory network incorporating regulations mediated by small RNA and protein-protein interactions

Corynebacterium glutamicum is a Gram-positive bacterium found in soil where the condition changes demand plasticity of the regulatory machinery. The study of such machinery at the global scale has been challenged by the lack of data integration. Here, we report three regulatory network models for C. glutamicum: strong (3040 interactions) constructed solely with regulations previously supported by directed experiments; all evidence (4665 interactions) containing the strong network, regulations previously supported by non-directed experiments, and protein-protein interactions with a direct effect on gene transcription; and sRNA (5222 interactions) containing the all evidence network and sRNA-mediated regulations. Compared to the previous version (2018), the strong and all evidence networks increased by 75 and 1225 interactions, respectively. We analyzed the system-level components of the three networks to identify how they differ and compared their structures against those for the networks of more than 40 species. The inclusion of the sRNAs regulations changed the proportions of the system-level components and increased the number of modules but decreased their size. The C. glutamicum regulatory structure contrasted with other bacterial regulatory networks. Finally, we used the strong networks of three model organisms to provide insights and future directions of the C. glutamicum regulatory network characterization.Comment: 32 pages, 4 figures, 1 supplementary materia

Macrociclo para el entrenamiento de la fuerza explosiva en el equipo de baloncesto de Holguín

According to the experience of the authors, the way to arrange the training in basketball, is maintained with traditional models or structures, so the objective of this work is to develop a training planning model, from a period direct to competition adapted to basketball for game positions (PDCABPJ), which corresponds to the modern criteria of training planning. To do this, different planning models that are framed between 1983 and 2009 were reviewed. In addition, the contents of the preparation were analyzed, and subsequently it was put into practice in the period from 2015 to 2017. Favorable results were reached from the qualitative and quantitative point of view of the basketball players by playing positions, which were submitted to the new structure.De acuerdo con la experiencia de los autores, la forma de estructurar el entrenamiento en el baloncesto, se mantiene con los modelos o estructuras tradicionales, por lo que objetivo de este trabajo es elaborar un modelo de planificación del entrenamiento, a partir de un periodo directo a competencia adaptado al baloncesto por posiciones de juego (PDCABPJ), que se corresponda con los criterios modernos de la planificación del entrenamiento. Para ello, se revisaron diferentes modelos de planificación que se enmarcan entre los años 1983 y 2009. Además, se analizaron los contenidos de la preparación, y posteriormente el mismo fue puesto en práctica en el período del 2015 al 2017. Se arribaron a resultados favorables desde el punto de vista cualitativo y cuantitativo de los baloncestistas por posiciones de juego, que se sometieron a la nueva estructura

ASPECTOS REPRODUCTIVOS DEL PEJERREY PATAGÓNICO (Odontesthes hatcheri Eigenmann, 1909) EN EL EMBALSE EZEQUIEL RAMOS MEXIA (ARGENTINA)

Objetivo. Evaluar la efectividad anestésica de 2-fenoxietanol, benzocaina, quinaldina y metanosulfonato de tricaina (MS-222) en alevinos y juveniles de cachama blanca (Piaractus brachypomus). Materiales y métodos. Alevinos (7.3 ± 6.8 g) y juveniles (49.6 ± 28.4 g) fueron expuestos a 2-fenoxietanol (200, 400 y 600 ppm), benzocaina (50, 100 y 150 ppm), quinaldina (7.5, 15 y 30 ppm) o MS-222 (100, 150 y 200 ppm) (n=12), para evaluar la duración de la inducción y recuperación, frecuencia opercular y la sobrevivencia post-anestesia. Resultados. El desarrollo corporal y la concentración afectaron el periodo de inducción, siendo mayor en juveniles que en alevinos (p0.05), mientras que juveniles expuestos a MS-222 mostraron el menor tiempo de inducción con la menor concentración (

Functional architecture of Escherichia coli: new insights provided by a natural decomposition approach

The E. coli transcriptional regulatory network is shown to have a nonpyramidal architecture of independent modules governed by transcription factors, whose responses are integrated by intermodular genes

Partition Quantitative Assessment (PQA): A quantitative methodology to assess the embedded noise in clustered omics and systems biology data

Identifying groups that share common features among datasets through clustering analysis is a typical problem in many fields of science, particularly in post-omics and systems biology research. In respect of this, quantifying how a measure can cluster or organize intrinsic groups is important since currently there is no statistical evaluation of how ordered is, or how much noise is embedded in the resulting clustered vector. Many of the literature focuses on how well the clustering algorithm orders the data, with several measures regarding external and internal statistical measures; but none measure has been developed to statistically quantify the noise in an arranged vector posterior a clustering algorithm, i.e., how much of the clustering is due to randomness. Here, we present a quantitative methodology, based on autocorrelation, to assess this problem.Comment: 9 pages, 6 figure

Software de gestión de equipamiento biomédico para pequeñas y medianas instituciones de la salud

Se ha detectado que las instituciones de la salud encuadradas en la actual resolución 267/2003 aprobada por el Ministerio de Salud de Argentina hasta el nivel 7, carecen de un sistema de gestión de equipamiento biomédico debido a la falta de oferta en el mercado y alto costo de desarrollo que, solo pueden afrontar grandes hospitales. Generando desconocimiento sobre el equipamiento existente y falta de mantenimiento preventivo y correctivo, creando riesgos y gastos evitables. Por ello se ha desarrollado un software, denominado Maguss, para suplir esta necesidad, utilizando una plataforma web, con los objetivos de obtener bajo costo de implementación, utilizar el equipamiento existente, nula inversión, acceso remoto desde diversos dispositivos móviles y alto grado de seguridad, destinado a pequeñas y medianas instituciones. La implementación en dos nosocomios de la ciudad de Córdoba dio excelentes resultados, permitiendo confirmar la necesidad de este tipo de sistemas y determinar las mejoras futuras que tendrán la colaboración del departamento de informática médica de la Facultad de Ciencias Exactas, Físicas y Naturales de la misma ciudad.Sociedad Argentina de Informática e Investigación Operativ

Analysis of strawberry ripening by dynamic speckle measurements

This work seeks to determine the age of a fruit from observation of its dynamic speckle pattern. A mobile speckle pattern originates on the fruit's surface due to the interference of the wavefronts reflected from moving scatterers. For this work we analyzed two series of photographs of a strawberry speckle pattern, at different stages of ripening, acquired with a CMOS camera. The first day, we took ten photographs at an interval of one second. The same procedure was repeated the next day. From each series of images we extracted several statistical descriptors of pixel-to-pixel gray level variation during the observation time. By comparing these values from the first to the second day we noticed a diminution of the speckle activity. This decay demonstrated that after only one day the ripening process of the strawberry can be detected by dynamic speckle pattern analysis. For this study we employed a simple new algorithm to process the data obtained from the photographs. This algorithm allows defining a global mobility index that indicates the evolution of the fruit's ripening.Fil: Mulone, C.. Universidad Tecnológica Nacional. Facultad Regional Paraná; ArgentinaFil: Budini, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Tecnológica Nacional. Facultad Regional Paraná; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Santa Fe. Instituto de Física del Litoral; ArgentinaFil: Vincitorio, F. M.. Universidad Tecnológica Nacional. Facultad Regional Paraná; ArgentinaFil: Freyre, C.. Universidad Tecnológica Nacional. Facultad Regional Paraná; ArgentinaFil: López Díaz, A. J.. Universidad da Coruña; España;Fil: Ramil Rego, A.. Universidad da Coruña; España