Etude des paramètres sériques biochimiques : le cas des lapins (Néozelandais – cunistar) de Côte d\'ivoire.

une insuffisance des bases données. Les valeurs obtenues sur l\'ensemble des sérums des animaux ont permis d\'observer les moyennes au niveau de la glycémie (0,79 ± 0,18 g/l) et des métabolites tels que, l\'urée (0,42 ± 0,10 g/l), la créatinine (6,88 ± 1,66 mg/l), l\'acide urique (5,42 3,75 mg/l), le cholestérol total (0,55 ± 0,13 mg/l), les triglycérides (2,18 ± 1,23 g/l), les protéines totales (26,00 ± 15,16 g/l), les bilirubines totales (7,25 ± 1,07 mg/l) et les bilirubines directes 1,58 ± 0,38 mg/l. Concernant les enzymes, les déterminations des valeurs ont été faites pour la transaminase alanine - aminotransférase (45,52 ± 20,54 UI/l), la transaminase aspartate - aminotransférase (21,24 ± 9,89 UI/l), les phosphatases alcalines (432,66 ± 207,8 UI/l), la ã Glutamyl transférase (24,24 ± 15,21 UI/l), les créatine phosphokinases (954 ± 343,4 UI/l), les lactates déshydrogénases (1135 ± 335,93 UI/l) et enfin les amylases (114,72 ± 27,99 UI/l). Par ailleurs, les moyennes des ions ont été déterminées pour le calcium (94 ± 4,43 mg/l), le magnésium (15,72 ± 2,49 mg/ l), le phosphore (26,70 ± 10,51 mg/l), le fer sérique (1,33 ± 0,74 mg/l), le sodium (141,89 ± 3,96 mg/l), le potassium (3,89 ± 0,38 mg/l) et enfin le chlore (100,85 ± 3,04 mg/l). En conclusion, les valeurs obtenues en zone tropicale nécessitent une évaluation avec un échantillonnage plus grand pour des comparaisons avec des données européennesStudy of biochemical serum constituents among rabbits (Néozélandais-cunistar) in Côte d\'Ivoire is carried out as a result insufficiency of available data. Values acquired from all animals serum allowed to look at glycemia (0.79 ± 0.18 g/l) and metabolites related averages such as, urea (0.42 ± 0.10 g/l), creatinine (6.88 ± 1.66 mg/l), uric acid (5.42 ± 3.75 mg/l), total cholesterol (0.55 ± 0.13 mg/l), triglycol (2.18 ± 1.23 g/l), total proteins (26.00 ± 15.16 g/l), total bilirubins (7.25 ± 1.07 mg/l) and bilirubins direct (1.58 ± 0.38 mg/l). Concerning enzymes, averages were worked out for alanine - aminotransferase (45.52 ± 20.54 UI/l), aspartate - aminotransferase (21.24 ± 9.89 UI/l), phosphatases alkaline (432.66 ± 207.8 UI/l), ã Glutamyl transférase (24.24 ± 15.21 UI/l), créatine kinase (954 ± 343.4 UI/l), lactate deshydrogenase (1135 ± 335.93 UI/l) and the amylases (114.72 ± 27.99 UI/l). Besides as to ions, the averages were determined for calcium (94 ± 4.43 mg/l), magnesium (15.72 ± 2.49 mg/l), phosphor (26.70 ± 10.51 mg/l), iron serum (1.33 ± 0.74 mg/l), sodium (141.89 ± 3.96 mg/l), potassium (3.89 ± 0.38 mg/l) and chlorine (100.85 ± 3.04 mg/l). In short, the values obtained in tropical area require assessment with a larger sampling for comparisons with European data. Keywords: paramètr.es sériques, Néozélandais -Cunistar, lapin./serum parameters, Néozélandais - Cunistar, rabbit.Sciences & Nature Vol. 4 (1) 2007: pp. 37-4

Protein crystals in adenovirus type 5-infected cells: requirements for intranuclear crystallogenesis, structural and functional analysis

Intranuclear crystalline inclusions have been observed in the nucleus of epithelial cells infected with Adenovirus serotype 5 (Ad5) at late steps of the virus life cycle. Using immuno-electron microscopy and confocal microscopy of cells infected with various Ad5 recombinants modified in their penton base or fiber domains, we found that these inclusions represented crystals of penton capsomers, the heteromeric capsid protein formed of penton base and fiber subunits. The occurrence of protein crystals within the nucleus of infected cells required the integrity of the fiber knob and part of the shaft domain. In the knob domain, the region overlapping residues 489–492 in the FG loop was found to be essential for crystal formation. In the shaft, a large deletion of repeats 4 to 16 had no detrimental effect on crystal inclusions, whereas deletion of repeats 8 to 21 abolished crystal formation without altering the level of fiber protein expression. This suggested a crucial role of the five penultimate repeats in the crystallisation process. Chimeric pentons made of Ad5 penton base and fiber domains from different serotypes were analyzed with respect to crystal formation. No crystal was found when fiber consisted of shaft (S) from Ad5 and knob (K) from Ad3 (heterotypic S5-K3 fiber), but occurred with homotypic S3K3 fiber. However, less regular crystals were observed with homotypic S35-K35 fiber. TB5, a monoclonal antibody directed against the Ad5 fiber knob was found by immunofluorescence microscopy to react with high efficiency with the intranuclear protein crystals in situ. Data obtained with Ad fiber mutants indicated that the absence of crystalline inclusions correlated with a lower infectivity and/or lower yields of virus progeny, suggesting that the protein crystals might be involved in virion assembly. Thus, we propose that TB5 staining of Ad-infected 293 cells can be used as a prognostic assay for the viability and productivity of fiber-modified Ad5 vectors

Genetic diversity and structure of Iberian Peninsula cowpeas compared to world-wide cowpea accessions using high density SNP markers

Cowpea (Vigna unguiculata L. Walp) is an important legume crop due to its high protein content, adaptation to heat and drought and capacity to fix nitrogen. Europe has a deficit of cowpea production. Knowledge of genetic diversity among cowpea landraces is important for the preservation of local varieties and is the basis to obtain improved varieties. The aims of this study were to explore diversity and the genetic structure of a set of Iberian Peninsula cowpea accessions in comparison to a worldwide collection and to infer possible dispersion routes of cultivated cowpea.This study was supported by EUROLEGUME project. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 613781. European Investment Funds by FEDER/COMPETE/ POCI – Operational Competitiveness and Internationalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT – Portuguese Foundation for Science and Technology, under the project UID/AGR/04033/2013. MMA was partially supported by the Feed the Future Innovation Lab for Climate Resilient Cowpea (USAID Cooperative Agreement AID-OAA-A-13-00070), which is directed by TJC. The funding entities had no role in the design of the study, collection, analysis and interpretation of data, or in writing the manuscript.info:eu-repo/semantics/publishedVersio

Paediatric schistosomiasis:What we know and what we need to know

Schistosomiasis affects over 200 million people worldwide, most of whom are children. Research and control strategies directed at preschool-aged children (PSAC), i.e., ≤5 years old, have lagged behind those in older children and adults. With the recent WHO revision of the schistosomiasis treatment guidelines to include PSAC, and the recognition of gaps in our current knowledge on the disease and its treatment in this age group, there is now a concerted effort to address these shortcomings. Global and national schistosome control strategies are yet to include PSAC in treatment schedules. Maximum impact of schistosome treatment programmes will be realised through effective treatment of PSAC. In this review, we (i) discuss the current knowledge on the dynamics and consequences of paediatric schistosomiasis and (ii) identify knowledge and policy gaps relevant to these areas and to the successful control of schistosome infection and disease in this age group. Herein, we highlight risk factors, immune mechanisms, pathology, and optimal timing for screening, diagnosis, and treatment of paediatric schistosomiasis. We also discuss the tools required for treating schistosomiasis in PSAC and strategies for accessing them for treatment