A systematic review on the ecosystem services provided by green infrastructure

Urbanization and climate change are endangering the sustainability of public spaces through increased land artificialization, ecological fragmentation, reduced resource availability, and limited accessibility to natural and seminatural areas. Properly managing Green Infrastructure (GI) can contribute to mitigating these challenges by delivering multiple provisioning, regulating, supporting and cultural Ecosystem Services (ES). This would facilitate the implementation of strategically planned GI networks in cities for urban regeneration purposes. In this context, this study developed a systematic review on the ES provided by GI using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. The analysis of 199 eligible articles indicated that more efforts should be made to address more ES at once, which connects to the need for conceiving GI as a strategically planned network of areas aimed at delivering diverse benefits. Based on the methods used in the items reviewed, geoprocessing tools and multi-criteria decision analysis are proposed to develop systems of indicators capable of accounting for multiple ES. These systems should also rely on multidisciplinary and participative procedures to encompass various facets of GI and represent the priorities of all relevant stakeholders

Key processes for Cheirolophus (Asteraceae) diversification on oceanic islands inferred from AFLP data

The radiation of the genus Cheirolophus (Asteraceae) in Macaronesia constitutes a spectacular case of rapid diversification on oceanic islands. Twenty species - nine of them included in the IUCN Red List of Threatened Species - have been described to date inhabiting the Madeiran and Canarian archipelagos. A previous phylogenetic study revealed that the diversification of Cheirolophus in Macaronesia started less than 2 Ma. As a result of such an explosive speciation process, limited phylogenetic resolution was reported, mainly due to the low variability of the employed molecular markers. In the present study, we used highly polymorphic AFLP markers to i) evaluate species' boundaries, ii) infer their evolutionary relationships and iii) investigate the patterns of genetic diversity in relation to the potential processes likely involved in the radiation of Cheirolophus. One hundred and seventy-two individuals representing all Macaronesian Cheirolophus species were analysed using 249 AFLP loci. Our results suggest that geographic isolation played an important role in this radiation process. This was likely driven by the combination of poor gene flow capacity and a good ability for sporadic long-distance colonisations. In addition, we also found some traces of introgression and incipient ecological adaptation, which could have further enhanced the extraordinary diversification of Cheirolophus in Macaronesia. Last, we hypothesize that current threat categories assigned to Macaronesian Cheirolophus species do not reflect their respective evolutionary relevance, so future evaluations of their conservation status should take into account the results presented here

Methylglyoxal Produced by Amyloid- Peptide-Induced Nitrotyrosination of Triosephosphate Isomerase Triggers Neuronal Death in Alzheimer’s Disease

Amyloid-β peptide (Aβ) aggregates induce nitro-oxidative stress, contributing to the characteristic neurodegeneration found in Alzheimer's disease (AD). One of the most strongly nitrotyrosinated proteins in AD is the triosephosphate isomerase (TPI) enzyme which regulates glycolytic flow, and its efficiency decreased when it is nitrotyrosinated. The main aims of this study were to analyze the impact of TPI nitrotyrosination on cell viability and to identify the mechanism behind this effect. In human neuroblastoma cells (SH-SY5Y), we evaluated the effects of Aβ42 oligomers on TPI nitrotyrosination. We found an increased production of methylglyoxal (MG), a toxic byproduct of the inefficient nitro-TPI function. The proapoptotic effects of Aβ42 oligomers, such as decreasing the protective Bcl2 and increasing the proapoptotic caspase-3 and Bax, were prevented with a MG chelator. Moreover, we used a double mutant TPI (Y165F and Y209F) to mimic nitrosative modifications due to Aβ action. Neuroblastoma cells transfected with the double mutant TPI consistently triggered MG production and a decrease in cell viability due to apoptotic mechanisms. Our data show for the first time that MG is playing a key role in the neuronal death induced by Aβ oligomers. This occurs because of TPI nitrotyrosination, which affects both tyrosines associated with the catalytic center

A precision medicine test predicts clinical response after idarubicin and cytarabine induction therapy in AML patients

Complete remission (CR) after induction therapy is the first treatment goal in acute myeloid leukemia (AML) patients and has prognostic impact. Our purpose is to determine the correlation between the observed CR/CRi rate after idarubicin (IDA) and cytarabine (CYT) 3 + 7 induction and the leukemic chemosensitivity measured by an ex vivo test of drug activity. Bone marrow samples from adult patients with newly diagnosed AML were included in this study. Whole bone marrow samples were incubated for 48 h in well plates containing IDA, CYT, or their combination. Pharmacological response parameters were estimated using population pharmacodynamic models. Patients attaining a CR/CRi with up to two induction cycles of 3 + 7 were classified as responders and the remaining as resistant. A total of 123 patients fulfilled the inclusion criteria and were evaluable for correlation analyses. The strongest clinical predictors were the area under the curve of the concentration response curves of CYT and IDA. The overall accuracy achieved using MaxSpSe criteria to define positivity was 81%, predicting better responder (93%) than non-responder patients (60%). The ex vivo test provides better yet similar information than cytogenetics, but can be provided before treatment representing a valuable in-time addition. After validation in an external cohort, this novel ex vivo test could be useful to select AML patients for 3 + 7 regimen vs. alternative schedules

A precision medicine test predicts clinical response after idarubicin and cytarabine induction therapy in AML patients

Complete remission (CR) after induction therapy is the first treatment goal in acute myeloid leukemia (AML) patients and has prognostic impact. Our purpose is to determine the correlation between the observed CR/CRi rate after idarubicin (IDA) and cytarabine (CYT) 3 + 7 induction and the leukemic chemosensitivity measured by an ex vivo test of drug activity. Bone marrow samples from adult patients with newly diagnosed AML were included in this study. Whole bone marrow samples were incubated for 48 h in well plates containing IDA, CYT, or their combination. Pharmacological response parameters were estimated using population pharmacodynamic models. Patients attaining a CR/CRi with up to two induction cycles of 3 + 7 were classified as responders and the remaining as resistant. A total of 123 patients fulfilled the inclusion criteria and were evaluable for correlation analyses. The strongest clinical predictors were the area under the curve of the concentration response curves of CYT and IDA. The overall accuracy achieved using MaxSpSe criteria to define positivity was 81%, predicting better responder (93%) than non-responder patients (60%). The ex vivo test provides better yet similar information than cytogenetics, but can be provided before treatment representing a valuable in-time addition. After validation in an external cohort, this novel ex vivo test could be useful to select AML patients for 3 + 7 regimen vs. alternative schedules

Bone Marrow-Derived Cells from Male Donors Do Not Contribute to the Endometrial Side Population of the Recipient

Accumulated evidence demonstrates the existence of bone marrow-derived cells origin in the endometria of women undergoing bone marrow transplantation (BMT). In these reports, cells of a bone marrow (BM) origin are able to differentiate into endometrial cells, although their contribution to endometrial regeneration is not yet clear. We have previously demonstrated the functional relevance of side population (SP) cells as the endogenous source of somatic stem cells (SSC) in the human endometrium. The present work aims to understand the presence and contribution of bone marrow-derived cells to the endometrium and the endometrial SP population of women who received BMT from male donors. Five female recipients with spontaneous or induced menstruations were selected and their endometrium was examined for the contribution of XY donor-derived cells using fluorescent in situ hybridization (FISH), telomapping and SP method investigation. We confirm the presence of XY donor-derived cells in the recipient endometrium ranging from 1.7% to 2.62%. We also identify 0.45–0.85% of the donor-derived cells in the epithelial compartment displaying CD9 marker, and 1.0–1.83% of the Vimentin-positive XY donor-derived cells in the stromal compartment. Although the percentage of endometrial SP cells decreased, possibly being due to chemotherapy applied to these patients, they were not formed by XY donor-derived cells, donor BM cells were not associated with the stem cell (SC) niches assessed by telomapping technique, and engraftment percentages were very low with no correlation between time from transplant and engraftment efficiency, suggesting random terminal differentiation. In conclusion, XY donor-derived cells of a BM origin may be considered a limited exogenous source of transdifferentiated endometrial cells rather than a cyclic source of BM donor-derived stem cells

On the actual nature of the anti-ferromagnetism shown by unrestricted calculations on conjugated hydrocarbon rings

Unrestricted Hartree-Fock (UHF) calculations with spin-polarisation, plus Møller-Plesset (MP) perturbative corrections, have been applied to the study of the first four molecules of the family of n-acenes and also to the ground state of cyclobutadiene. The results are similar to those previously obtained for n-periacenes, which describe anti-ferromagnetic spin-polarised electronic structures. The projection to pure spin states gives results that recover the usual description of these molecules, i.e., non spin-polarised ground states. Extended coupled cluster calculations reinforce the projected results, also giving non spin-polarised ground states

Rendimiento del cultivo de amaranto (Amaranthus hypochondriacus L.) ante el ataque de insectos barrenadores y defoliadores, en la Zona centro de Córdoba, Argentina

El amaranto, por su amplia plasticidad agroecológica, es un cultivo que permite diversificar los sistemas de producción estivales. Entre las especies de insectos que podrían considerarse plagas potenciales por su abundancia, daño que ocasionan y frecuencia de aparición, se señalan a coleópteros barrenadores Conotrachelus histrio (Boheman), Conotrachelus cervinus (Hustache) y Aerenea quadriplagiata (Boheman) y lepidópteros defoliadores Achyra bifidalis (F.) y Achyra similalis Guenée. No se han realizado evaluaciones de pérdidas en el cultivo causadas por estos insectos. El objetivo de este trabajo fue estimar el rendimiento del cultivo de amaranto ante el ataque de las especies de fitófagos mencionadas. El ensayo se realizó en el Campo Escuela de la FCA-UNC durante la campaña 2018-2019. Las unidades experimentales fueron parcelas de 7 surcos de 5 m de largo separados entre sí por 0,35 m. Se siguió un diseño en bloques al azar con tres repeticiones. Los tratamientos fueron T: testigo sin aplicación, T1: clorantraniliprole 20% SC (30 cc/ha) para control de lepidópteros y T2: lambdacialotrina 5% EC (400cc/ha) para control total de insectos. Las aplicaciones se realizaron a los 15 días de emergencia del cultivo y posteriormente con frecuencia quincenal hasta madurez fisiológica. Una vez que el cultivo alcanzo su madurez de cosecha se procedió a elegir 5 panojas al azar de cada uno de los tratamientos; las que fueron cosechadas manualmente para luego desgranarlas y pesarlas Cabe mencionar que el cultivo fue afectado por la caída de granizo (07/02/19) en su etapa reproductiva. Los rendimientos obtenidos fueron T: 23,41 g/m², T1: 26,16 g/m² y T2: 22,72 g/m². Se realizó un ANAVA y Test de comparación de medias de Tuckey (α=0,05). Si bien se observó una tendencia a valores más altos en T1, no se encontraron diferencias significativas para la variable rendimiento en este estudio preliminar.Fil: Moscardó, María Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Grosso, Gerardo Mario. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Conles, Martha Yolanda. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Gerard, F.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Sánchez, E.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Sánchez, N.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Fichetti, P.. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaVIII Jornadas Integradas de Investigación, Extensión y Enseñanza de la Facultad de Ciencias AgropecuariasCórdobaArgentinaUniversidad Nacional de Córdoba. Facultad de Ciencias Agropecuaria

Performance of correlation functionals in ab initio chemisorption cluster-model calculations: Alkali metals on Si(111)

The performance of different correlation functionals has been tested for alkali metals, Li to Cs, interacting with cluster models simulating different active sites of the Si(111) surface. In all cases, the ab initio Hartree-Fock density has been obtained and used as a starting point. The electronic correlation energy is then introduced as an a posteriori correction to the Hartree-Fock energy using different correlation functionals. By making use of the ionic nature of the interaction and of different dissociation limits we have been able to prove that all functionals tested introduce the right correlation energy, although to a different extent. Hence, correlation functionals appear as an effective and easy way to introduce electronic correlation in the ab initio Hartree-Fock description of the chemisorption bond in complex systems where conventional configuration interaction techniques cannot be used. However, the calculated energies may differ by some tens of eV. Therefore, these methods can be employed to get a qualitative idea of how important correlation effects are, but they have some limitations if accurate binding energies are to be obtained