Competition between benthic cyanobacteria and diatoms as influenced by different grain sizes and temperatures

An experimental laboratory set-up was used to study the influence of different grain size compositions and temperatures on the growth of benthic cyanobacteria and diatoms, and on the competition between these 2 groups. Monospecific cultures of 3 species of cyanobacteria (Merismopedia punctata, Microcoleus chthonoplastes, Oscillatoria limosa), and of 2 species of benthic diatoms (Phaeodactylum tricornutum and Nitzschia sp.) were used. The organisms were cultured in 100 ml flasks filled with medium and 3 different kinds of sediment: (1) Sand (fine sand, 63 to 200 µm), (2) Mud-I (mixed fine sand and mud <63 µm in the ratio 80:20 wt %), (3) Mud-II (mixed fine sand and mud in the ratio 50:50 wt %). Experimental temperatures were 10, 15 and 25°C. At 10°C and 15°C, both diatom species achieved the highest biomass on the sediments of the finest grain size (50 wt % < 63 µm) while cyanobacteria achieved low biomass levels. Coarsening of sediments at the same temperature levels revealed a gradually lower biomass of the diatoms. Particularly on sand, the diatoms never reached the same concentrations of chlorophyll a as on mud. The cyanobacteria, on the other hand, had the highest biomass on sand at 15°C. In the competition experiments the benthic diatom species Nitzschia sp. dominated all types of sediments at 10°C and 15°C. The experiments at 25°C were dominated by the filamentous cyanobacterium M. chthonoplastes. This indicates the importance of abiotic conditions for the distribution and abundance of benthic phototrophic micro-organisms

Geomagnetic disturbances at high latitudes during very low solar wind density event

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/94969/1/grl13480.pd

Performance of Different Diagnostic PD-L1 Clones in Head and Neck Squamous Cell Carcinoma

Background: The approval of immune checkpoint inhibitors in combination with specific diagnostic biomarkers presents new challenges to pathologists as tumor tissue needs to be tested for expression of programmed death-ligand 1 (PD-L1) for a variety of indications. As there is currently no requirement to use companion diagnostic assays for PD-L1 testing in Germany different clones are used in daily routine. While the correlation of staining results has been tested in various entities, there is no data for head and neck squamous cell carcinomas (HNSCC) so far. Methods: We tested five different PD-L1 clones (SP263, SP142, E1L3N, 22-8, 22C3) on primary HNSCC tumor tissue of 75 patients in the form of tissue microarrays. Stainings of both immune and tumor cells were then assessed and quantified by pathologists to simulate real-world routine diagnostics. The results were analyzed descriptively and the resulting staining pattern across patients was further investigated by principal component analysis and non-negative matrix factorization clustering. Results: Percentages of positive immune and tumor cells varied greatly. Both the resulting combined positive score as well as the eligibility for certain checkpoint inhibitor regimens was therefore strongly dependent on the choice of the antibody. No relevant co-clustering and low similarity of relative staining patterns across patients was found for the different antibodies. Conclusions: Performance of different diagnostic anti PD-L1 antibody clones in HNSCC is less robust and interchangeable compared to reported data from other tumor entities. Determination of PD-L1 expression is critical for therapeutic decision making and may be aided by back-to-back testing of different PD-L1 clones

Photometric AGN reverberation mapping - an efficient tool for BLR sizes, black hole masses and host-subtracted AGN luminosities

Photometric reverberation mapping employs a wide bandpass to measure the AGN continuum variations and a suitable band, usually a narrow band (NB), to trace the echo of an emission line in the broad line region (BLR). The narrow band catches both the emission line and the underlying continuum, and one needs to extract the pure emission line light curve. We performed a test on two local AGNs, PG0003+199 (=Mrk335) and Ark120, observing well-sampled broad- (B, V) and narrow-band light curves with the robotic 15cm telescope VYSOS-6 on Cerro Armazones, Chile. In PG0003+199, H_alpha dominates the flux in the NB by 85%, allowing us to measure the time lag of H_alpha against B without the need to correct for the continuum contribution. In Ark120, H_beta contributes only 50% to the flux in the NB. The cross correlation of the B and NB light curves shows two distinct peaks of similar strength, one at lag zero from the autocorrelated continuum and one from the emission line at tau_cent = 47.5 +/- 3.4 days. We constructed a synthetic H_beta light curve, by subtracting a scaled V light curve, which traces the continuum, from the NB light curve. The cross correlation of this synthetic H_beta light curve with the B light curve shows only one major peak at tau_cent = 48.0 +/- 3.3 days, while the peak from the autocorrelated continuum at lag zero is absent. We conclude that, as long as the emission line contributes at least 50% to the bandpass, the pure emission line light curve can be reconstructed from photometric monitoring data so that the time lag can be measured. For both objects the lags we find are consistent with spectroscopic reverberation results. While the dense sampling (median 2 days) enables us to determine tau_cent with small (10%) formal errors, we caution that gaps in the light curves may lead to much larger systematic uncertainties. (Abstract shortened, see the manuscript.)Comment: 12 pages, 15 figures, accepted for publication in Astronomy and Astrophysic

Impact of the 26-30 May 2003 solar events on the earth ionosphere and thermosphere.

During the last week of May 2003, the solar active region AR 10365 produced a large number of flares, several of which were accompanied by Coronal Mass Ejections (CME). Specifically on 27 and 28 May three halo CMEs were observed which had a significant impact on geospace. On 29 May, upon their arrival at the L1 point, in front of the Earth's magnetosphere, two interplanetary shocks and two additional solar wind pressure pulses were recorded by the ACE spacecraft. The interplanetary magnetic field data showed the clear signature of a magnetic cloud passing ACE. In the wake of the successive increases in solar wind pressure, the magnetosphere became strongly compressed and the sub-solar magnetopause moved inside five Earth radii. At low altitudes the increased energy input to the magnetosphere was responsible for a substantial enhancement of Region-1 field-aligned currents. The ionospheric Hall currents also intensified and the entire high-latitude current system moved equatorward by about 10°. Several substorms occurred during this period, some of them - but not all - apparently triggered by the solar wind pressure pulses. The storm's most notable consequences on geospace, including space weather effects, were (1) the expansion of the auroral oval, and aurorae seen at mid latitudes, (2) the significant modification of the total electron content in the sunlight high-latitude ionosphere, (3) the perturbation of radio-wave propagation manifested by HF blackouts and increased GPS signal scintillation, and (4) the heating of the thermosphere, causing increased satellite drag. We discuss the reasons why the May 2003 storm is less intense than the October-November 2003 storms, although several indicators reach similar intensities

Improved diagnostics targeting c-MET in non-small cell lung cancer: expression, amplification and activation?

Background: Several c-MET targeting inhibitory molecules have already shown promising results in the treatment of patients with Non-small Cell Lung Cancer (NSCLC). Combination of EGFR-and c-MET-specific molecules may overcome EGFR tyrosine kinase inhibitor (TKI) resistance. The aim of this study was to allow for the identification of patients who might benefit from TKI treatments targeting MET and to narrow in on the diagnostic assessment of MET. Methods: 222 tumor tissues of patients with NSCLC were analyzed concerning c-MET expression and activation in terms of phosphorylation (Y1234/1235 and Y1349) using a microarray format employing immunohistochemistry (IHC). Furthermore, protein expression and MET activation was correlated with the amplification status by Fluorescence in Situ Hybridization (FISH). Results: Correlation was observed between phosphorylation of c-MET at Y1234/1235 and Y1349 (spearman correlation coefficient r(s) = 0.41;p 0.05). c-MET gene amplification was detected in eight of 214 patients (3.7 %). No significant association was observed between c-MET amplification, c-MET protein expression and phosphorylation. Conclusion: Our data indicate, that neither expression of c-MET nor the gene amplification status might be the best way to select patients for MET targeting therapies, since no correlation with the activation status of MET was observed. We propose to take into account analyzing the phosphorylation status of MET by IHC to select patients for MET targeting therapies. Signaling of the receptor and the activation of downstream molecules might be more crucial for the benefit of therapeutics targeting MET receptor tyrosine kinases than expression levels alone

Coordinated observation of field line resonance in the mid-tail

Standing Alfv&amp;#233;n waves of 1.1 mHz (~15 min in period) were observed by the Cluster satellites in the mid-tail during 06:00-07:00&amp;nbsp;UT on 8&amp;nbsp;August 2003. Pulsations with the same frequency were also observed at several ground stations near Cluster&apos;s footpoint. The standing wave properties were determined from the electric and magnetic field measurements of Cluster. Data from the ground magnetometers indicated a latitudinal amplitude and phase structure consistent with the driven field line resonance (FLR) at 1.1 mHz. Simultaneously, quasi-periodic oscillations at different frequencies were observed in the post-midnight/early morning sector by GOES&amp;nbsp;12 (&lt;i&gt;l&lt;/i&gt;&lt;sub&gt;0&lt;/sub&gt;&amp;asymp;8.7), Polar (&lt;i&gt;l&lt;/i&gt;&lt;sub&gt;0&lt;/sub&gt;&amp;asymp;11-14) and Geotail (&lt;i&gt;l&lt;/i&gt;&lt;sub&gt;0&lt;/sub&gt;&amp;asymp;9.8). The 8&amp;nbsp;August 2003 event yields rare and interesting datasets. It provides, for the first time, coordinated in situ and ground-based observations of a very low frequency FLR in the mid-tail on stretched field lines