61 research outputs found

    Spectrometric Analyses, Structure and Voltammetric Study of Nickel(II) with N[(1E) Phenylmethylene N2[2(2Hydroxyphenylmethylene]Amino ethyl) Imidazolidin-1-yl Ethylamine

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    The reaction of N[(1E) phenylmethylene N2[2(2hydroxyphenylmethylene]amino ethyl) imidazolidin -1-yl ethylamine ligand (H 3 L) with nickel(II) salt, by heating at 40°C and addition of NaOH in excess, was made by eliminating of the phenol substituted imidazolidine ring, giving the mononuclear nickel(II) complex of the deprotonated bis-salicylaldehyde-triethylenetetramine (L −2 ). UV-Vis, FTIR and structural resolution show an octahedral geometry for [NiL]·6H 2 O complex. This compound has been characterized by single crystal X-ray diffraction study. This technique reveals that Ni-H 2 L involves a high-spin nickel(II) ion within a pseudo-octahedral geometry. The Ni(II) complex has NiN 4 O 2 coordination sphere as established from a crystal structure determination. The crystals of Ni(II) complex are tetragonal, space group P4/ncc, a = b = 19.348 A; c = 13.201 A. R(F) value (0.0528) found shows a very good precision of the determined geometrical parameters. Cyclic voltammetry of nickel complex is indicative of electronic communication between the nickel center via Schiff base ligand. The results obtained confirm the imidazolidine ring-cleavage reaction and the elimination of the substituted phenol on this ring and show that the presence of H 3 L ligand around the metallic center stabilizes the oxidation of the Ni(II) to Ni(III)

    Acetonic Extract of Buxus sempervirens Induces Cell Cycle Arrest, Apoptosis and Autophagy in Breast Cancer Cells

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    Plants are an invaluable source of potential new anti-cancer drugs. Here, we investigated the cytotoxic activity of the acetonic extract of Buxus sempervirens on five breast cancer cell lines, MCF7, MCF10CA1a and T47D, three aggressive triple positive breast cancer cell lines, and BT-20 and MDA-MB-435, which are triple negative breast cancer cell lines. As a control, MCF10A, a spontaneously immortalized but non-tumoral cell line has been used. The acetonic extract of Buxus sempervirens showed cytotoxic activity towards all the five studied breast cancer cell lines with an IC50 ranging from 7.74 µg/ml to 12.5 µg/ml. Most importantly, the plant extract was less toxic towards MCF10A with an IC50 of 19.24 µg/ml. Fluorescence-activated cell sorting (FACS) analysis showed that the plant extract induced cell death and cell cycle arrest in G0/G1 phase in MCF7, T47D, MCF10CA1a and BT-20 cell lines, concomitant to cyclin D1 downregulation. Application of MCF7 and MCF10CA1a respective IC50 did not show such effects on the control cell line MCF10A. Propidium iodide/Annexin V double staining revealed a pre-apoptotic cell population with extract-treated MCF10CA1a, T47D and BT-20 cells. Transmission electron microscopy analyses indicated the occurrence of autophagy in MCF7 and MCF10CA1a cell lines. Immunofluorescence and Western blot assays confirmed the processing of microtubule-associated protein LC3 in the treated cancer cells. Moreover, we have demonstrated the upregulation of Beclin-1 in these cell lines and downregulation of Survivin and p21. Also, Caspase-3 detection in treated BT-20 and T47D confirmed the occurrence of apoptosis in these cells. Our findings indicate that Buxus sempervirens extract exhibit promising anti-cancer activity by triggering both autophagic cell death and apoptosis, suggesting that this plant may contain potential anti-cancer agents for single or combinatory cancer therapy against breast cancer

    Targeting ion channels for cancer treatment : current progress and future challenges

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    Palladium-Catalyzed Direct Heteroarylation of Chloropyridines and Chloroquinolines

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    Easy technique for calculating productivity index of horizontal wells

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    In recent years, horizontal well technology have evolved as the more favorable option in the state of Kuwait over the conventional vertical and deviated wells. Several models have been published in the literature to estimate the productivity index of horizontal wells. Generally, all of these models require two factors which are the shape and pseudo-skin factors. Also, most of these models require parameters that are not always easy to determine.This study presents easy and quick technique for calculating the productivity index of a horizontal well. The new technique has been established based on the instantaneous source solutions for the pressure response of a horizontal well. The pseudo-steady state flow is expected to develop because the horizontal well is assumed to be acting in finite reservoirs. Two parameters were derived and their influences on the productivity index were investigated. The first one is the pseudo-skin factor due to asymmetry of a horizontal well. The second one is the shape factor group.The study emphasizes that the productivity index for horizontal wells are strongly affected by the two parameters: the shape factor group and the pseudo-skin factor. Shape factor group is mainly affected by the drainage area configuration while pseudo-skin factor is mainly affected by vertical penetration. The study confirms that the productivity index is affected by the penetration ratio in the horizontal plane and reservoir geometry. In addition, square-shaped reservoir produces at maximum productivity index while channel-shaped reservoir produces at minimum productivity index. The study finds that wellbore eccentricity (wellbore location in the horizontal plane) does not affect the pseudo-skin factor and vertical penetration ratio does not affect the shape factor group. The results obtained from the new technique have been compared with the results from Babu Odeh model and Economides model. Numerical examples will be included in the paper
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