Morpho-molecular ex vivo detection and grading of non-muscle-invasive bladder cancer using forward imaging probe based multimodal optical coherence tomography and Raman spectroscopy

Non-muscle-invasive bladder cancer affects millions of people worldwide, resulting in significant discomfort to the patient and potential death. Today, cystoscopy is the gold standard for bladder cancer assessment, using white light endoscopy to detect tumor suspected lesion areas, followed by resection of these areas and subsequent histopathological evaluation. Not only does the pathological examination take days, but due to the invasive nature, the performed biopsy can result in significant harm to the patient. Nowadays, optical modalities, such as optical coherence tomography (OCT) and Raman spectroscopy (RS), have proven to detect cancer in real time and can provide more detailed clinical information of a lesion, e.g. its penetration depth (stage) and the differentiation of the cells (grade). In this paper, we present an ex vivo study performed with a combined piezoelectric tube-based OCT-probe and fiber optic RS-probe imaging system that allows large field-of-view imaging of bladder biopsies, using both modalities and co-registered visualization, detection and grading of cancerous bladder lesions. In the present study, 119 examined biopsies were characterized, showing that fiber-optic based OCT provides a sensitivity of 78% and a specificity of 69% for the detection of non-muscle-invasive bladder cancer, while RS, on the other hand, provides a sensitivity of 81% and a specificity of 61% for the grading of low- and high-grade tissues. Moreover, the study shows that a piezoelectric tube-based OCT probe can have significant endurance, suitable for future long-lasting in vivo applications. These results also indicate that combined OCT and RS fiber probe-based characterization offers an exciting possibility for label-free and morpho-chemical optical biopsies for bladder cancer diagnostics. © 2020 The Royal Society of Chemistry

Material reutilization cycles across industries and production lines

The concept of Industrial Symbiosis aims at organizing industrial activity like a living ecosystem where the by-product outputs of one process are used as valuable raw material input for another process. A significant method for the systematic planning of Industrial Symbiosis is found in input–output matching, which is aimed at collecting material input and output data from companies, and using the results to establish links across industries. The collection and classification of data is crucial to the development of synergies in Industrial Symbiosis. Public and private institutions involved in the planning and development of Industrial Symbiosis rely however on manual interpretation of information in the course of creating synergies. Yet, the evaluation and analysis of these data sources on Industrial Symbiosis topics is a tall order. Within this chapter a method is presented which describes value creation activities according to the Value Creation Module (VCM). They are assessed before they are integrated in Value Creation Networks (VCNs), where alternative uses for by-products are proposed by means of iterative input-output matching of selected value creation factors

Quince (Cydonia oblonga) in vitro plant root formation through an automated temporary inmersion system, and its acclimation

Artículo científicoQuince (Cydonia oblonga) is a non-traditional fruit tree found in Costa Rica that has therapeutic and nutritional properties; however its slow growth and root formation prevents the production of a homogeneous population when using conventional farming techniques. Hence, the aim of this research project was to generate uniform plant material in a reduced time span using a temporary immersion bioreactor system (RITAS ®). A semisolid rooting MS culture medium supplemented with 0.1 mg L-1 NAA; 0.3 mg L-1 IBA and 3% sucrose (pH 6.5), developed in the Centro de Investigación en Biotecnología (CIB), Instituto Tecnológico de Costa Rica (ITCR), in Cartago, was used as a reference medium. Four different variations in the sucrose concentration (1%, 2%, 3%, and 4%) were performed in liquid medium. Each trial was evaluated with in vitro plants which had been previously exposed to the culture medium of the corresponding treatments, in a stationary mode and for a 15 day long period, and with in vitro plants without any previous treatment (a total of eight treatments). The comparison of the root formation percentages evidenced the clear effect of sucrose concentration used, with the best results obtained when using the 2% sucrose trial with no pre-treatment (73.3%). The in vitro plants were acclimated in cylinders made out of peat, have previously been disinfected with fungicide, and placed in a humidity chamber at a 20.5°C average temperature and a 75,5% relative humidity for the establishment of weekly fertilizing cycles. The acclimation process generated an 80% survival rate, since several seedlings experienced stem strangulation caused by a fungal attack. The conidiophores identified through optical and scanning electron microscopy evidenced the presence of Cladosporium spp., which was controlled with carbendazim and iprodione fungicides

Integrating Human-Centred Design Approach into Sustainable-Oriented 3D Printing Systems

Modern 3D printing systems have become pervasive and widely used both in professional and in informal contexts, including sustainable-oriented ones. However, the risk to create very effective but non-sustainable solutions is very high since 3D printing systems could potentially increase the environmental emergencies and the unsustainable growth. In the transition process toward sustainable ways of production and consumption, the so-called human factor still plays an important role in the achievement of sustainable-oriented actions; it drives the adoption of proper lifestyles that directly and indirectly influence the ways through which such technologies are used. Therefore, future Sustainable 3D Printing Systems should integrate the humans in the systems’ development. This study presents two important results: (a) it presents a set of interdisciplinary ‘Sustainable 3D Printing Systems’, which compose a promising sustainable-oriented scenario useful to support the transition processes toward sustainable designs and productions, and (b) it proposes a new strategy for the integration of human-centred aspects into Sustainable 3D Printing Systems, by combining insights from human-centred design approach

Preclinical Evaluation of Caprylic Acid-Fractionated IgG Antivenom for the Treatment of Taipan (Oxyuranus scutellatus) Envenoming in Papua New Guinea

articulo (arbitrado) -- Universidad de Costa Rica, Instituto de Investigaciones Clodomiro Picado, 2011Background: Snake bite is a common medical emergency in Papua New Guinea (PNG). The taipan, Oxyuranus scutellatus, inflicts a large number of bites that, in the absence of antivenom therapy, result in high mortality. Parenteral administration of antivenoms manufactured in Australia is the current treatment of choice for these envenomings. However, the price of these products is high and has increased over the last 25 years; consequently the country can no longer afford all the antivenom it needs. This situation prompted an international collaborative project aimed at generating a new, low-cost antivenom against O. scutellatus for PNG. Methodology/Principal Findings: A new monospecific equine whole IgG antivenom, obtained by caprylic acid fractionation of plasma, was prepared by immunising horses with the venom of O. scutellatus from PNG. This antivenom was compared with the currently used F(ab’)2 monospecific taipan antivenom manufactured by CSL Limited, Australia. The comparison included physicochemical properties and the preclinical assessment of the neutralisation of lethal neurotoxicity and the myotoxic, coagulant and phospholipase A2 activities of the venom of O. scutellatus from PNG. The F(ab’)2 antivenom had a higher protein concentration than whole IgG antivenom. Both antivenoms effectively neutralised, and had similar potency, against the lethal neurotoxic effect (both by intraperitoneal and intravenous routes of injection), myotoxicity, and phospholipase A2 activity of O. scutellatus venom. However, the whole IgG antivenom showed a higher potency than the F(ab’)2 antivenom in the neutralisation of the coagulant activity of O. scutellatus venom from PNG. Conclusions/Significance: The new whole IgG taipan antivenom described in this study compares favourably with the currently used F(ab’)2 antivenom, both in terms of physicochemical characteristics and neutralising potency. Therefore, it should be considered as a promising low-cost candidate for the treatment of envenomings by O. scutellatus in PNG, and is ready to be tested in clinical trials.This study was supported by Vicerrectoría de Investigación, Universidad de Costa Rica (project 741-A9-003); the PNG Office of Higher Education, CTP Limited (Milne Bay Estates), and the Australian Venom Research Unit (University of Melbourne), which is funded by the Australian Government Department of Health and Ageing, the Australia Pacific Science Foundation and Snowy Nominees. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP

Jaminan Perlindungan Hak Pilih dan Kewajiban Negara Melindungi Hak Pilih Warga Negara dalam Konstitusi (Kajian Kritis Pemilu Serentak 2019)

Human rights are a fundamental right that has been carried out by a human being while guarantees in the state in protection and regulation must be guaranteed in a fundamental law, namely the constitution of a country in Indonesia itself in the protection of human rights in Article 1 article (2) Article 28 Article 2 Paragraph (1) Article 6 A (1) Article 9 Paragraph (1) Article 22 (c) In essence the protection of the right to vote and be elected by the state is absolutely done while the implementation is stated in Law No. 39 of 1999 concerning Human Rights In article 23 which reads: everyone is free to choose and and has political beliefs, that means the protection of voting rights and voting has been carried out in the Indonesian state's legal politics in practice the suffrage is implemented by the General Election Commission in implementing the right to vote and be elected this is very difficult because the population system in Indonesia is still inadequate as a result of the right to vote this is not obtained by the citizens themselves

Jaminan Perlindungan Hak Pilih dan Kewajiban Negara Melindungi Hak Pilih Warga Negara dalam Konstitusi (Kajian Kritis Pemilu Serentak 2019)

Human rights are a fundamental right that has been carried out by a human being while guarantees in the state in protection and regulation must be guaranteed in a fundamental law, namely the constitution of a country in Indonesia itself in the protection of human rights in Article 1 article (2) Article 28 Article 2 Paragraph (1) Article 6 A (1) Article 9 Paragraph (1) Article 22 (c) In essence the protection of the right to vote and be elected by the state is absolutely done while the implementation is stated in Law No. 39 of 1999 concerning Human Rights In article 23 which reads: everyone is free to choose and and has political beliefs, that means the protection of voting rights and voting has been carried out in the Indonesian state's legal politics in practice the suffrage is implemented by the General Election Commission in implementing the right to vote and be elected this is very difficult because the population system in Indonesia is still inadequate as a result of the right to vote this is not obtained by the citizens themselves