Diffusion Staining Techniques

Cross-section staining of p-type diffusions was investigated. The method employed a stain formulation of Diffusion depths of 1.9 microns were delineated and photographed using a scanning electron microscope

Farnham rolls investigation

The object of this experiment was to investigate the possibility of obtaining pre-setting data for the 1 Farnham Rolls'. The experiment was carried out by applying certain deflections and measuring the resulting radii of curvature. Thus curves of curvature against deflection were produced for different sheet widths, and from these curves attempts to produce a conical frustum with prescribed radii were made. The results obtained can not be applied to conical parts, but this test served to indicate that it is possible to obtain pre-setting data for various applications

Aerobic capacity and telomere length in human skeletal muscle and leukocytes across the lifespan

A reduction in aerobic capacity and the shortening of telomeres are hallmarks of the ageing process. We examined whether a lower aerobic capacity is associated with shorter TL in skeletal muscle and/or leukocytes, across a wide age range of individuals. We also tested whether TL in human skeletal muscle (MTL) correlates with TL in leukocytes (LTL). Eighty-two recreationally active, healthy men from the Gene SMART cohort (31.4±8.2 years; body mass index (BMI)=25.3±3.3kg/m2), and 11 community dwelling older men (74.2±7.5years-old; BMI=28.7±2.8kg/m2) participated in the study. Leukocytes and skeletal muscle samples were collected at rest. Relative telomere length (T/S ratio) was measured by RT-PCR. Associations between TL, aerobic capacity (VO2 peak and peak power) and age were assessed with robust linear models. Older age was associated with shorter LTL (45% variance explained, P<0.001), but not MTL (P= 0.7). Aerobic capacity was not associated with MTL (P=0.5), nor LTL (P=0.3). MTL and LTL were correlated across the lifespan (rs=0.26, P=0.03). In healthy individuals, age explain most of the variability of LTL and this appears to be independent of individual aerobic capacity. Individuals with longer LTL also have a longer MTL, suggesting that there might be a shared molecular mechanism regulating telomere length

Genetic testing for exercise prescription and injury prevention: AIS-Athlome consortium-FIMS joint statement

Abstract Background There has been considerable growth in basic knowledge and understanding of how genes are influencing response to exercise training and predisposition to injuries and chronic diseases. On the basis of this knowledge, clinical genetic tests may in the future allow the personalisation and optimisation of physical activity, thus providing an avenue for increased efficiency of exercise prescription for health and disease. Results This review provides an overview of the current status of genetic testing for the purposes of exercise prescription and injury prevention. As such there are a variety of potential uses for genetic testing, including identification of risks associated with participation in sport and understanding individual response to particular types of exercise. However, there are many challenges remaining before genetic testing has evidence-based practical applications; including adoption of international standards for genomics research, as well as resistance against the agendas driven by direct-to-consumer genetic testing companies. Here we propose a way forward to develop an evidence-based approach to support genetic testing for exercise prescription and injury prevention. Conclusion Based on current knowledge, there is no current clinical application for genetic testing in the area of exercise prescription and injury prevention, however the necessary steps are outlined for the development of evidence-based clinical applications involving genetic testing

Dreaming of drams: Authenticity in Scottish whisky tourism as an expression of unresolved Habermasian rationalities

In this paper, the production of whisky tourism at both independently owned and corporately owned distilleries in Scotland is explored by focusing on four examples (Arran, Glengoyne, Glenturret and Bruichladdich). In particular, claims of authenticity and Scottishness of Scottish whiskies through commercial materials, case studies, website-forum discussions and 'independent' writing about such whisky are analysed. It is argued that the globalisation and commodification of whisky and whisky tourism, and the communicative backlash to these trends typified by the search for authenticity, is representative of a Habermasian struggle between two irreconcilable rationalities. This paper will demonstrate that the meaning and purpose of leisure can be understood through such explorations of the tension between the instrumentality of commodification and the freedom of individuals to locate their own leisure lives in the lifeworld that remains. © 2011 Taylor & Francis

Acoustic characteristics of a ported shroud turbocompressor operating at design conditions

[EN] In this article, the acoustic characterisation of a turbocharger compressor with ported shroud design is carried out through the numerical simulation of the system operating under design conditions of maximum isentropic efficiency. While ported shroud compressors have been proposed as a way to control the flow near unstable conditions in order to obtain a more stable operation and enhance deep surge margin, it is often assumed that the behaviour under stable design conditions is characterised by a smooth, non-detached flow that matches an equivalent standard compressor. Furthermore, research is scarce regarding the acoustic effects of the ported shroud addition, especially under the design conditions. To analyse the flow field evolution and its relation with the noise generation, spectral signatures using statistical and scale-resolving turbulence modelling methods are obtained after successfully validating the performance and acoustic predictions of the numerical model with experimental measurements. Propagation of the frequency content through the ducts has been estimated with the aid of pressure decomposition methods to enhance the content coming from the compressor. Expected acoustic phenomena such as `buzz-saw¿ tones, blade passing peaks and broadband noise are correctly identified in the modelled spectrum. Analysis of the flow behaviour in the ported shroud shows rotating structures through the slot that may impact the acoustic and vibration response. Further inspection of the pressure field through modal decomposition confirms the influence of the ported shroud cavity in noise generation and propagation, especially at lower frequencies, suggesting that further research should be carried out on the impact these flow enhancement solutions have on the noise emission of the turbocharger.The project was sponsored and supported by BorgWarner Turbo Systems and the Regional Growth Fund (RGF Grant Award 01.09.07.01/1789C). The authors would like to thank BorgWarner Turbo Systems for permission to publish the results presented in this article. The support of the HPC group at the University of Huddersfield is gratefully acknowledged.Sharma, S.; Broatch, A.; Garcia Tiscar, J.; Allport, JM.; Nickson, AK. (2020). Acoustic characteristics of a ported shroud turbocompressor operating at design conditions. International Journal of Engine Research. 21(8):1454-1468. https://doi.org/10.1177/1468087418814635S14541468218Sundström, E., Semlitsch, B., & Mihăescu, M. (2017). Generation Mechanisms of Rotating Stall and Surge in Centrifugal Compressors. Flow, Turbulence and Combustion, 100(3), 705-719. doi:10.1007/s10494-017-9877-zGonzalez, A., Ferrer, M., de Diego, M., Piñero, G., & Garcia-Bonito, J. . (2003). Sound quality of low-frequency and car engine noises after active noise control. Journal of Sound and Vibration, 265(3), 663-679. doi:10.1016/s0022-460x(02)01462-1Brizon, C. J. da S., & Bauzer Medeiros, E. (2012). Combining subjective and objective assessments to improve acoustic comfort evaluation of motor cars. Applied Acoustics, 73(9), 913-920. doi:10.1016/j.apacoust.2012.03.013Teng, C., & Homco, S. (2009). Investigation of Compressor Whoosh Noise in Automotive Turbochargers. SAE International Journal of Passenger Cars - Mechanical Systems, 2(1), 1345-1351. doi:10.4271/2009-01-2053Figurella, N., Dehner, R., Selamet, A., Tallio, K., Miazgowicz, K., & Wade, R. (2014). Noise at the mid to high flow range of a turbocharger compressor. Noise Control Engineering Journal, 62(5), 306-312. doi:10.3397/1/376229Torregrosa, A. J., Broatch, A., Margot, X., García-Tíscar, J., Narvekar, Y., & Cheung, R. (2017). Local flow measurements in a turbocharger compressor inlet. Experimental Thermal and Fluid Science, 88, 542-553. doi:10.1016/j.expthermflusci.2017.07.007Broatch, A., Galindo, J., Navarro, R., García-Tíscar, J., Daglish, A., & Sharma, R. K. (2015). Simulations and measurements of automotive turbocharger compressor whoosh noise. Engineering Applications of Computational Fluid Mechanics, 9(1), 12-20. doi:10.1080/19942060.2015.1004788Raitor, T., & Neise, W. (2008). Sound generation in centrifugal compressors. Journal of Sound and Vibration, 314(3-5), 738-756. doi:10.1016/j.jsv.2008.01.034Galindo, J., Tiseira, A., Navarro, R., & López, M. A. (2015). Influence of tip clearance on flow behavior and noise generation of centrifugal compressors in near-surge conditions. International Journal of Heat and Fluid Flow, 52, 129-139. doi:10.1016/j.ijheatfluidflow.2014.12.004Broatch, A., Galindo, J., Navarro, R., & García-Tíscar, J. (2014). Methodology for experimental validation of a CFD model for predicting noise generation in centrifugal compressors. International Journal of Heat and Fluid Flow, 50, 134-144. doi:10.1016/j.ijheatfluidflow.2014.06.006Semlitsch, B., & Mihăescu, M. (2016). Flow phenomena leading to surge in a centrifugal compressor. Energy, 103, 572-587. doi:10.1016/j.energy.2016.03.032Sundström, E., Semlitsch, B., & Mihăescu, M. (2018). Acoustic signature of flow instabilities in radial compressors. Journal of Sound and Vibration, 434, 221-236. doi:10.1016/j.jsv.2018.07.040Torregrosa, A. J., Broatch, A., Margot, X., & García-Tíscar, J. (2016). Experimental methodology for turbocompressor in-duct noise evaluation based on beamforming wave decomposition. Journal of Sound and Vibration, 376, 60-71. doi:10.1016/j.jsv.2016.04.035Nicoud, F., & Ducros, F. (1999). Flow, Turbulence and Combustion, 62(3), 183-200. doi:10.1023/a:1009995426001Chow, P., Cross, M., & Pericleous, K. (1996). A natural extension of the conventional finite volume method into polygonal unstructured meshes for CFD application. Applied Mathematical Modelling, 20(2), 170-183. doi:10.1016/0307-904x(95)00156-eKaji, S., & Okazaki, T. (1970). Generation of sound by rotor-stator interaction. Journal of Sound and Vibration, 13(3), 281-307. doi:10.1016/s0022-460x(70)80020-7Sivagnanasundaram, S., Spence, S., & Early, J. (2013). Map Width Enhancement Technique for a Turbocharger Compressor. Journal of Turbomachinery, 136(6). doi:10.1115/1.4007895Aubry, N. (1991). On the hidden beauty of the proper orthogonal decomposition. Theoretical and Computational Fluid Dynamics, 2(5-6), 339-352. doi:10.1007/bf00271473Wold, S., Esbensen, K., & Geladi, P. (1987). Principal component analysis. Chemometrics and Intelligent Laboratory Systems, 2(1-3), 37-52. doi:10.1016/0169-7439(87)80084-9LIANG, Y. C., LEE, H. P., LIM, S. P., LIN, W. Z., LEE, K. H., & WU, C. G. (2002). PROPER ORTHOGONAL DECOMPOSITION AND ITS APPLICATIONS—PART I: THEORY. Journal of Sound and Vibration, 252(3), 527-544. doi:10.1006/jsvi.2001.4041Abdi, H., & Williams, L. J. (2010). Principal component analysis. Wiley Interdisciplinary Reviews: Computational Statistics, 2(4), 433-459. doi:10.1002/wics.101Nikiforov, V. (2007). The energy of graphs and matrices. Journal of Mathematical Analysis and Applications, 326(2), 1472-1475. doi:10.1016/j.jmaa.2006.03.07

Transgenic expression of human signal regulatory protein alpha in Rag2−/−γc −/− mice improves engraftment of human hematopoietic cells in humanized mice

Transplantation of human hematopoietic stem cells into severely immunocompromised newborn mice allows the development of a human hematopoietic and immune system in vivo. NOD/scid/γc−/− (NSG) and BALB/c Rag2−/ −γc−/− mice are the most commonly used mouse strains for this purpose and a number of studies have demonstrated the high value of these model systems in areas spanning from basic to translational research. However, limited cross-reactivity of many murine cytokines on human cells and residual host immune function against the xenogeneic grafts results in defective development and maintenance of human cells in vivo. Whereas NSG mice have higher levels of absolute human engraftment than similar mice on a BALB/c background, they have a shorter lifespan and NOD ES cells are unsuitable for the complex genetic engineering that is required to improve human hematopoiesis and immune responses by transgenesis or knockin of human genes. We have generated mice that faithfully express a transgene of human signal regulatory protein alpha (SIRPa), a receptor that negatively regulates phagocytosis, in Rag2−/−γc−/− mice on a mixed 129/BALB/c background, which can easily be genetically engineered. These mice allow significantly increased engraftment and maintenance of human hematopoietic cells reaching levels comparable to NSG mice. Furthermore, we found improved functionality of the human immune system in these mice. In summary, hSIRPa-transgenic Rag2−/−γc−/− mice represent a unique mouse strain supporting high levels of human cell engraftment, which can easily be genetically manipulated

A mouse model for the human pathogen Salmonella typhi

Salmonella enterica serovar Typhi (S. Typhi) causes typhoid fever, a life-threatening human disease. The lack of animal models due to S. Typhi's strict human host specificity has hindered its study and vaccine development. We find that immunodeficient Rag2(-/-) γc(-/-) mice engrafted with human fetal liver hematopoietic stem and progenitor cells are able to support S. Typhi replication and persistent infection. A S. Typhi mutant in a gene required for virulence in humans was unable to replicate in these mice. Another mutant unable to produce typhoid toxin exhibited increased replication, suggesting a role for this toxin in the establishment of persistent infection. Furthermore, infected animals mounted human innate and adaptive immune responses to S. Typhi, resulting in the production of cytokines and pathogen-specific antibodies. We expect that this mouse model will be a useful resource for understanding S. Typhi pathogenesis and for evaluating potential vaccine candidates against typhoid fever

Human IL-3/GM-CSF knock-in mice support human alveolar macrophage development and human immune responses in the lung

Mice with a functional human immune system have the potential to allow in vivo studies of human infectious diseases and to enable vaccine testing. To this end, mice need to fully support the development of human immune cells, allow infection with human pathogens, and be capable of mounting effective human immune responses. A major limitation of humanized mice is the poor development and function of human myeloid cells and the absence of human immune responses at mucosal surfaces, such as the lung. To overcome this, we generated human IL-3/GM-CSF knock-in (hIL-3/GM- CSF KI) mice. These mice faithfully expressed human GM-CSF and IL-3 and developed pulmonary alveolar proteinosis because of elimination of mouse GM-CSF. We demonstrate that hIL-3/GM-CSF KI mice engrafted with human CD34+ hematopoietic cells had improved human myeloid cell reconstitution in the lung. In particular, hIL-3/GM-CSF KI mice supported the development of human alveolar macrophages that partially rescued the pulmonary alveolar proteinosis syndrome. Moreover, human alveolar macrophages mounted correlates of a human innate immune response against influenza virus. The hIL-3/GM-CSF KI mice represent a unique mouse model that permits the study of human mucosal immune responses to lung pathogens

Right Ventricular Adaptation Is Associated with the Glu298Asp Variant of the NOS3 Gene in Elite Athletes

Nitric oxide (NO), an important endogenous pulmonary vasodilator is synthetized by the endothelial NO synthase (NOS3). Reduced NO bioavailability and thus the Glu298Asp polymorphism of NOS3 may enhance right ventricular (RV) afterload and hypertrophic remodeling and influence athletic performance. To test this hypothesis world class level athletes (water polo players, kayakers, canoeists, rowers, swimmers, n = 126) with a VO2 maximum greater than 50ml/kg/min were compared with non-athletic volunteers (n = 155). Cardiopulmonary exercise tests and cardiac magnetic resonance imaging (cMRI) were performed to determine structural or functional changes. Genotype distribution of the NOS3 Glu298Asp polymorphism was not affected by gender or physical performance. Cardiac MRI showed increased stroke volume with eccentric hypertrophy in all athletes regardless of their genotype. However, the Asp allelic variant carriers had increased RV mass index (32+/-6g versus 27+/-6g, p<0.01) and larger RV stroke volume index (71+/-10ml versus 64+/-10ml, p<0.01) than athletes with a Glu/Glu genotype. Genotype was not significantly associated with athletic performance. In the non-athletic group no genotype related differences were detected. The association between the NOS3 Glu298Asp polymorphism and RV structure and dimension in elite athletes emphasizes the importance of NOS3 gene function and NO bioavailability in sport related cardiac adaptation