DETERMINACIÓN DEL SISTEMA DE COMPETENCIAS DEL PROCESO DE POSGRADO EN CENTROS DE INVESTIGACIÓN. CASO DE ESTUDIO: INSTITUTO DE CIENCIA ANIMAL

Much has been said about the importance of human capital in organizations and especially in the research centers, which exhibit growing needs to guarantee people's skill training. The objective of this study was to determine the competence system of the Postgraduate Process in the Institute of Animal Science, using techniques and tools such as: Critical Incident Interview and Critical Examination. As a result, 6 competences representative of the Postgraduate process were obtained with values ​​of agreement higher than 65%, expressing agreement among the experts in the definition of: Professional rigor, Ethic, Teamwork, and Search for excellence, Interest in keeping up to date and Innovation and creativity. The key positions for the process and the associated competencies for the superior performance of said positions were also defined. The competences identified can serve as a reference to other centers.Mucho se ha hablado de la importancia que tiene el capital humano en las organizaciones y en especial en los centros de investigación, los que exhiben necesidades crecientes de garantizar la formación por competencias de las personas. El trabajo tiene como objetivo determinar el sistema de competencias del proceso de Posgrado en el Instituto de Ciencia Animal, a partir de técnicas y herramientas tales como: Entrevista de Incidente Crítico y Examen Crítico. Como resultado se obtuvieron 6 competencias representativas del proceso de Posgrado con valores de concordancia superiores al 65%, expresando acuerdo entre los expertos en la definición de: Rigor profesional, Ética, Trabajo en equipo, Búsqueda de la excelencia, Interés por mantenerse actualizado e Innovación y creatividad. Se definieron además los cargos claves para el proceso y las competencias asociadas para el desempeño superior de dichos cargos. Las competencias identificadas pueden servir de referencia a otros centros

Zephyramina . Alcaloide novedoso aislado de los bulbos de Zephyrantes citrina Baker

Muchas especies de plantas de la familia Amaryllidaceae son usadas en la medicina popular contra diferentes enfermedades, principalmente como citotóxicas, entre ellas se destaca el uso de varias especies de Zephyrantes en diferentes regiones del mundo. Se encontró actividad citotóxica in vitro en células KB (carcinoma de labio humano) en los extractos alcohólicos obtenidos de los bulbos de la planta Zephyrantes citrina Baker, recolectados en Caibarien (Cuba), lo cual motivó realizar su estudio fitoquímico. En este artículo se reporta el aislamiento y caracterización de cinco alcaloides de esta planta. Los alcaloides licorina y haemanthamina (comunes en las plantas de la familia Amaryllidaeeae) fueron identificados por sus constantes físicas, análisis espectroscópico y comparación con muestra auténtica. La estructura de cavinina (primera vez en el género) fue establecida por sus constantes físicas y análisis espectroscópico (IR, de masas y RMN-'H) . Mientras que joubertiamina fue identificada por sus constantes físicas y datos espectroscópicos (IR, de masas), este compuesto pertenece al grupo de la mesembrina y es la cuarta ocasión en que se aísla un alcaloide de este grupo en Amaryllidaceae . La estructura del alcaloide novedoso zephyramina fue establecida a partir de sus constantes físicas y análisis espectroscópico (IR, de masas, RMN-'H y RMN-'"C), pertenece a la serie de la crinina sin sustituyente hidroxilo en el puente etilénico, anillo oxirano en los CiCe y sustituyentes metoxilos en el anillo A

HMGB Proteins from Yeast to Human. Gene Regulation, DNA Repair and Beyond

HMGB proteins are characterized for containing one or more HMG-box domains and are well conserved from yeasts to higher eukaryotes. The HMG-box domain is formed by three α-helices with an L-shaped fold. Although HMGB proteins also have cytoplasmic and extracellular functions, they bind to nuclear or mitochondrial DNA in a highly dynamic process that affects chromatin organization. In this review, we mainly focus on HMGB proteins from yeast and their human homologs as functionally involved in DNA repair and transcriptional regulation. Recent research reveals that these proteins participate in epigenetic control of gene expression, aging, disease, or stem-cell biology

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Molecular dynamics and NMR analysis of the configurational C assignment of epimeric 22,23-epoxides of stigmasterol

The determination of the stereochemistry of brasinosteroid analogs with 22,23-epoxide groups can be easily achieved by means of 13C NMR spectroscopy. Here, we provide a rationalization of the 13C chemical shift pattern found in 22R,23R- and 22S,23S-epoxides of stigmasterol, based on the analysis of γ effects. (22S,23S)- and (22R,23R)-3β-acetoxystigmast-22,23-epoxy-5,6β-diol were used in the study as model compounds. Our methodology starts with a conformational search by means of molecular dynamics and NMR (NOE contacts) spectroscopy, which is followed by the analysis of the different γ interactions affecting the chemical shift of interest. We demonstrate that the differences between the 13C chemical shift patterns of 22R,23R and 22S,23S isomers arise from γ effects as the result of diverging local conformations around the C17−C20 and C20−C22 bonds.Depto. de Química FísicaFac. de Ciencias QuímicasTRUEpu

Las Hurdes y su cultura

Recopilación de materiales (cantares, decires, poesías, cuentos, refranes, adivinanzas, juegos, etc.) acerca de distintos aspectos culturales de la mítica región altoextremeña: Las Hurdes. Los objetivos del trabajo son: conocer y recuperar la Cultura Hurdana, conservar y difundir las tradiciones, el folklore, las narraciones orales y las poesías, así como trasladar al aula una forma de vida. Se ha seguido una metodología basada en la participación y la cooperación, en relación con el contexto social y escolar y se ha procurado la participación de los alumnos y de sus familias a través de la recuperación de cuentos, refranes, poemas y canciones.ExtremaduraES

Pseudomonas aeruginosa antibiotic susceptibility profiles, genomic epidemiology and resistance mechanisms: a nation-wide five-year time lapse analysisResearch in context

Summary: Background: Pseudomonas aeruginosa healthcare-associated infections are one of the top antimicrobial resistance threats world-wide. In order to analyze the current trends, we performed a Spanish nation-wide high-resolution analysis of the susceptibility profiles, the genomic epidemiology and the resistome of P. aeruginosa over a five-year time lapse. Methods: A total of 3.180 nonduplicated P. aeruginosa clinical isolates from two Spanish nation-wide surveys performed in October 2017 and 2022 were analyzed. MICs of 13 antipseudomonals were determined by ISO-EUCAST. Multidrug resistance (MDR)/extensively drug resistance (XDR)/difficult to treat resistance (DTR)/pandrug resistance (PDR) profiles were defined following established criteria. All XDR/DTR isolates were subjected to whole genome sequencing (WGS). Findings: A decrease in resistance to all tested antibiotics, including older and newer antimicrobials, was observed in 2022 vs 2017. Likewise, a major reduction of XDR (15.2% vs 5.9%) and DTR (4.2 vs 2.1%) profiles was evidenced, and even more patent among ICU isolates [XDR (26.0% vs 6.0%) and DTR (8.9% vs 2.6%)] (p < 0.001). The prevalence of Extended-spectrum β-lactamase/carbapenemase production was slightly lower in 2022 (2.1%. vs 3.1%, p = 0.064). However, there was a significant increase in the proportion of carbapenemase production among carbapenem-resistant strains (29.4% vs 18.1%, p = 0.0246). While ST175 was still the most frequent clone among XDR, a slight reduction in its prevalence was noted (35.9% vs 45.5%, p = 0.106) as opposed to ST235 which increased significantly (24.3% vs 12.3%, p = 0.0062). Interpretation: While the generalized decrease in P. aeruginosa resistance, linked to a major reduction in the prevalence of XDR strains, is encouraging, the negative counterpart is the increase in the proportion of XDR strains producing carbapenemases, associated to the significant advance of the concerning world-wide disseminated hypervirulent high-risk clone ST235. Continued high-resolution surveillance, integrating phenotypic and genomic data, is necessary for understanding resistance trends and analyzing the impact of national plans on antimicrobial resistance. Funding: MSD and the Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea—NextGenerationEU

Analysis of Outcomes in Ischemic vs Nonischemic Cardiomyopathy in Patients With Atrial Fibrillation A Report From the GARFIELD-AF Registry

IMPORTANCE Congestive heart failure (CHF) is commonly associated with nonvalvular atrial fibrillation (AF), and their combination may affect treatment strategies and outcomes