Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Author's accepted version (post-print)

Skin mucus proteome map of European sea bass (Dicentrarchus labrax)

Author's accepted version (post-print).This is the peer reviewed version of the following article: Cordero, H., Brinchmann, M.F., Cuesta, A., Meseguer, J. & Esteban, M.A. (2015). Skin mucus proteome map of European sea bass (Dicentrarchus labrax). Proteomics, 15(23-24), 4007-4020, which has been published in final form at http://dx.doi.org/10.1002/pmic.201500120. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-820227.html)

Dietary Inclusion of Halobacterium salinarum Modulates Growth Performances and Immune Responses in Farmed Gilthead Seabream (Sparus aurata L.)

The use of natural immunostimulants is considered the most promising alternative to promote fish health, productive performance and quality, increasing the aquaculture profitability, sustainability and social acceptance. The purpose of this study was to evaluate the effect of the integration of a potential probiotic strain, Halobacterium salinarum, belonging to the Archaea domain, in the formulated diets of farmed gilthead seabream (Sparus aurata L.) in terms of growth performances and immunity responses. The experiment was set up to test two different levels of inclusion of the bacteria in the diet: 0.05% (D1) and 0.1% (D2). The effects on fish growth performances; humoral (peroxidase, protease, antiprotease and IgM levels) and cellular immunity parameters (phagocytosis, respiratory burst and myeloperoxidase), along with bactericidal activity, were evaluated after 15 and 30 days of experimental feeding. The obtained results showed that the inclusion of H. salinarum at the highest concentration (D2 0.1%) improved growth performances, bactericidal activity against Vibrio anguillarum and some parameters related both to the humoral and cellular immune response, suggesting exploring other aspects of welfare in view of future supplementations of this probiotic strain in the diet of S. aurata

Antifungal activity of silver nanoparticles in combination with ketoconazole against Malassezia furfur

Malassezia furfur is lipophilic and lipid-dependent yeast, inhabitant of human skin microbiota associated with several dermal disorders. In recent years, along with the advances in nanotechnology and the incentive to find new antimicrobialdrugs, there has been a growing interest in the utilization of nanoparticles for the treatment of skin microbial infections. This work aimed to study the in vitro inhibitory activity of silver nanoparticles (AgNP) against 41 M. furfurclinical isolates, visualize the interaction between AgNP-Malassezia, evaluate the synergism with ketoconazole (KTZ) and to produce an antimicrobial gel of AgNP?KTZ. The synthesized AgNP were randomly distributed around the yeastsurface and showed a fungicidal action with low minimal inhibitory concentration values. AgNP showed no antagonistic effect with KTZ. The broad-spectrum antimicrobial property with fungicidal action of AgNP and its accumulationin affected areas with a sustained release profile, added to the great antifungal activity of KTZ against Malassezia infections and other superficial mycoses, allowed us to obtain a gel based on carbopol formulated with AgNP?KTZwith the potential to improve the topical therapy of superficial malasseziosis, reduce the number of applications and, also, prevent the recurrence.Fil: Mussin, Javier Esteban. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Roldan, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; ArgentinaFil: Rojas, Florencia Dinorah. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Instituto de Medicina Regional; ArgentinaFil: Sosa, Maria de Los Angeles. Universidad Nacional del Nordeste. Instituto de Medicina Regional; ArgentinaFil: Pellegri, Nora Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; ArgentinaFil: Giusiano, Gustavo Emilio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentin

Dietary administration impact of olive pulp on growth performance, metabolic profile, immune status, and antioxidant potential of gilthead seabream (Sparus aurata L.)

The use of natural immunostimulants in aquaculture is intended to foster overall health and bolster resilience against diseases in farmed fish populations. It constitutes a crucial strategy that can contribute to securing the sustainability of the aquaculture industry, and it is an area that warrants ongoing exploration and development. Therefore, the aim of the present work was to evaluate the effect of dietary administration of olive pulp on growth rate, metabolic profile, serum antioxidant potential, and humoral and cellular innate immune parameters of gilthead seabream (Sparus aurata L.). For this, fish were fed control diet or olive pulp enriched diets (50, 100, and 200 mg kg feed−1) for 4 weeks. Our results demonstrated that the highest inclusion level improved the growth rates and the biological antioxidant potential in the serum of fish. However, after 4 weeks of feeding, most of the assayed metabolic parameters (Ca2+, TP, ALB, K+, and Na+) were increased in the serum of fish fed with a diet containing the lowest level of olive pulp (50 mg kg feed−1). Regarding the innate immune parameters, the IgM levels decreased in the serum of fish fed 50′s diet after 2 and 4 weeks of trial. However, the serum of fish fed with diets containing 100 and 200 showed an increase in hemolytic complement activity after 2 weeks whilst this increase was only sustained in the 200′s group after 4 weeks. After 2 weeks of feeding, the serum of the fish showed an increase in peroxidase activity due to the highest olive inclusion. Concerning cellular innate parameters, peroxidase activity, respiratory burst, and phagocytic ability were increased in head-kidney leucocytes of fish fed 100′s diet at 2 weeks compared to values from control fish. These results suggest that the administration of olive pulp-enriched diets can benefit fish growth, antioxidant, and immune status of gilthead seabream

TELEVISION AND CONSUMER THEORY: THE CASE OF MEXICAN SCHOOLCHILDREN

Introduction: The Pan American Health Organization in 2014 published strategic for the primary health care and healthy eating, for the prevention of malnutrition, including the restriction of advertising in foods with high-calorie schedule and kids channels. Television became the best choice for the promotion of these foods by the easy access that has in this group. Objective: To evaluate the degree of Association of the consumption of caloric food, broadcast television, and malnutrition through the theory of the consumption in school children of the school primary Miguel Hidalgo of the municipality of Temamatla, Mexico State. Method: Study transversal, descriptive, quantitative; shows statistics stratified by clusters of 140 schoolchildren. Instrument: "TV and consumption", Cronbach's Alpha of 0.789; data processed by the IBM SPSS Statistics 20 statistical package. Results: The direct relationship between consumption of caloric foods and the use of broadcast through the Association of P Pearson, with a contention between: 0.35, and greater than 0.05, playing: Association with positive linearity. Conclusions: Broadcast television keeps, a positive association with regard to consumption, preference and choice of food energy, promoted through advertising campaigns, based on the use of games, dreams, fantasies and characters from fashionable to establish long-term relationships between the school and the brand, which is part of an ephemeral happiness, conditioning perpetual stages of malnutrition.Introduction: The Pan American Health Organization in 2014 published strategic for the primary health care and healthy eating, for the prevention of malnutrition, including the restriction of advertising in foods with high-calorie schedule and kids channels. Television became the best choice for the promotion of these foods by the easy access that has in this group. Objective: To evaluate the degree of Association of the consumption of caloric food, broadcast television, and malnutrition through the theory of the consumption in school children of the school primary Miguel Hidalgo of the municipality of Temamatla, Mexico State. Method: Study transversal, descriptive, quantitative; shows statistics stratified by clusters of 140 schoolchildren. Instrument: "TV and consumption", Cronbach's Alpha of 0.789; data processed by the IBM SPSS Statistics 20 statistical package. Results: The direct relationship between consumption of caloric foods and the use of broadcast through the Association of P Pearson, with a contention between: 0.35, and greater than 0.05, playing: Association with positive linearity. Conclusions: Broadcast television keeps, a positive association with regard to consumption, preference and choice of food energy, promoted through advertising campaigns, based on the use of games, dreams, fantasies and characters from fashionable to establish long-term relationships between the school and the brand, which is part of an ephemeral happiness, conditioning perpetual stages of malnutrition

Espaciamientos para plantar eucaliptos en la Mesopotamia

El cultivo de Eucalyptus grandis, a partir de semilla y clones, se extiende en toda la Mesopotamia, donde las condiciones edafoclimáticas le permiten un buen desarrollo. La presencia de foresto-industrias que demandan materia prima para diferentes productos permite aprovechar de manera integral la madera producida. En este sentido, no toda la región posee similar presencia de industrias, ni en tipo ni en cantidad. Además, existen zonas que, por sus distancias a los centros de consumo de la madera producida, ciertos productos de bajo valor (en general productos finos), no son rentables, por lo que esquemas de menores densidades y manejos menos intensivos podrían ser recomendables. Por otro lado, la existencia de industrias que aprovechen principalmente productos finos (triturable/pulpable) requieren manejos tal vez poco intensivos, pero con densidades mayores.EEA ConcordiaFil: Garcia, Maria De Los Angeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Keller, Aldo Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; ArgentinaFil: Caniza, Federico Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentin

Design and Development of Soap Herbal Tablet of Larrea divaricata (Cav.) with Antifungal Activity Against Candida albicans

In this present work soap herbal tablet of Larrea divaricata Cav. ethanolic extract (LdE) with antifungal activity against Candida albicans, was designed and developed. The chemical profile of main compounds and quantification of nordihydroguaiaretic acid (NDGA) in the LdE was performed by means of UPLC-MS/MS. Two formulations (F1 and F2) were prepared by direct compression with different concentrations of active herbal ingredient (AHI). The performance of three carriers for impregnating LdE was evaluated. TRI-CAFOS® 500 was selected as carrier after a comparative study by Scanning Electron Microscope with AEROSIL® 200 and AEROPERL® 300. StarLac® 90, a coprocessed lactose, was employed as binder. The soap herbal tablets quality parameters were evaluated in accordance with United States Pharmacopeia (USP-43) guidelines, weight variation, hardness, friability, disintegration test, foam stability test and microbiological assay against candida albicans were performed. The F1 (LdE + TRI-CAFOS®500 (LdET1) 62.8%, StarLac®90 0.1%, Microcrystalline cellulose 10%, vitamin E 0.1%, Betan F® 5%, Lactic acid 5%, Rose essence 0.1%, Low density Hyaluronic acid 1.5% and magnesium stearate 0.5%) showed optimal rheological properties, good biopharmaceutical parameters (Disintegration test (27.33s), Hardness (3.00Kp), Friability (0.92%), Foam stability (1.12)) and Minimum inhibitory concentration (MIC) 25 mg/ml for Candida albicans. Considering that there are no soap herbal tablets on the market, the present design can be used as a novel herbal cosmetic form of vaginal hygiene.Fil: Jofre, Maria de Los Angeles. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Favier, Laura Silvina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Cianchino, Valeria Andrea. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Divizia, Maria Julia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ortiz, Javier Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Ortega, Claudia Alicia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentin